(Circular) (Six-base) MAPSORT of: pFN21AA1793.seq Check: 9090 from: 1 to: 8167 pFN21AA1793 8167 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4060 4490 4060 Size: 430 7737 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2840 5462 5977 278 Size: 53 83 186 936 1304 2622 515 2468 Fragments arranged by size: 2622 2468 1304 936 515 186 83 53 AccI GT'mk_AC Cuts at: 1937 5510 1937 Size: 3573 4594 Acc65I G'GTAC_C Cuts at: 5488 5488 Size: 8167 AclI AA'CG_TT Cuts at: 1563 6294 6667 1563 Size: 4731 373 3063 Fragments arranged by size: 4731 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2841 2943 3125 3173 3431 4554 4697 6230 Size: 1209 102 182 48 258 1123 143 1533 Cuts at: 6230 7614 7882 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1533 1384 1209 1123 268 258 182 143 102 48 AfeI AGC'GCT Cuts at: 4547 4547 Size: 8167 AflII C'TTAA_G Cuts at: 820 1017 2411 820 Size: 197 1394 6576 Fragments arranged by size: 6576 1394 197 AflIII A'CryG_T Cuts at: 1203 3302 3504 7066 8043 1203 Size: 2099 202 3562 977 1327 Fragments arranged by size: 3562 2099 1327 977 202 AgeI A'CCGG_T Cuts at: 6998 6998 Size: 8167 AhdI GACnn_n'nnGTC Cuts at: 6896 6896 Size: 8167 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8167 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8167 AlwNI CAG_nnn'CTG Cuts at: 2200 2461 2689 3117 3277 3340 4066 4506 5210 Size: 261 228 428 160 63 726 440 704 Cuts at: 5210 5910 7482 2200 Size: 700 1572 2885 Fragments arranged by size: 2885 1572 726 704 700 440 428 261 228 160 63 ApaI G_GGCC'C Cuts at: 2731 2731 Size: 8167 ApaLI G'TGCA_C Cuts at: 6224 7380 6224 Size: 1156 7011 ApoI r'AATT_y Cuts at: 1493 5476 5702 5854 7963 1493 Size: 3983 226 152 2109 1697 Fragments arranged by size: 3983 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6721 160 Size: 872 5689 1606 Fragments arranged by size: 5689 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8167 AvaI C'yCGr_G Cuts at: 1870 1943 2229 4938 5492 1870 Size: 73 286 2709 554 4545 Fragments arranged by size: 4545 2709 554 286 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4284 4317 4284 Size: 33 8134 BamHI G'GATC_C Cuts at: 5497 5864 5497 Size: 367 7800 BanI G'GyrC_C Cuts at: 618 943 1160 1787 5488 6943 618 Size: 325 217 627 3701 1455 1842 Fragments arranged by size: 3701 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2194 2280 2627 2731 3039 3168 3201 3561 Size: 803 86 347 104 308 129 33 360 Cuts at: 3561 3705 4107 4896 5486 1391 Size: 144 402 789 590 4072 Fragments arranged by size: 4072 803 789 590 402 360 347 308 144 129 104 86 33 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2559 3837 4542 5286 928 Size: 971 660 1278 705 744 3809 Fragments arranged by size: 3809 1278 971 744 705 660 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4505 4539 6358 6392 1903 Size: 34 50 34 2484 34 1819 34 3678 Fragments arranged by size: 3678 2484 1819 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3382 5011 6059 7269 3382 Size: 1629 1048 1210 4280 Fragments arranged by size: 4280 1629 1210 1048 BclI T'GATC_A Cuts at: 1548 4575 1548 Size: 3027 5140 BfrBI ATG'CAT Cuts at: 2935 2935 Size: 8167 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2312 4091 4397 Size: 107 122 71 631 605 640 1779 306 Cuts at: 4397 6778 136 Size: 2381 1525 Fragments arranged by size: 2381 1779 1525 640 631 605 306 122 107 71 BglII A'GATC_T Cuts at: 2770 8162 2770 Size: 5392 2775 BlpI GC'TnA_GC Cuts at: 3441 4527 5196 5584 3441 Size: 1086 669 388 6024 Fragments arranged by size: 6024 1086 669 388 Bme1580I G_kGCm'C Cuts at: 1790 2731 6228 7384 1790 Size: 941 3497 1156 2573 Fragments arranged by size: 3497 2573 1156 941 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8167 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5898 6856 442 Size: 416 53 83 399 24 4481 958 1753 Fragments arranged by size: 4481 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2300 4821 1056 Size: 1244 2521 4402 Fragments arranged by size: 4402 2521 1244 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2275 2611 2275 Size: 336 7831 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2198 2240 2303 2795 3269 3509 3539 3566 Size: 959 42 63 492 474 240 30 27 Cuts at: 3566 3728 4883 5075 6827 1239 Size: 162 1155 192 1752 2579 Fragments arranged by size: 2579 1752 1155 959 492 474 240 192 162 63 42 30 27 Bpu10I CC'TnA_GC Cuts at: 2193 4904 2193 Size: 2711 5456 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3023 3545 3785 4247 4349 5057 6292 7157 7455 Size: 522 240 462 102 708 1235 865 298 Cuts at: 7455 3023 Size: 3735 Fragments arranged by size: 3735 1235 865 708 522 462 298 240 102 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2219 2654 5033 6830 882 Size: 617 720 435 2379 1797 2219 Fragments arranged by size: 2379 2219 1797 720 617 435 BsaAI yAC'GTr Cuts at: 493 1204 5958 493 Size: 711 4754 2702 Fragments arranged by size: 4754 2702 711 BsaBI GATnn'nnATC Cuts at: 2588 8161 2588 Size: 5573 2594 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2837 4083 5304 5459 Size: 53 83 186 936 1304 1246 1221 155 Cuts at: 5459 5974 6356 6991 275 Size: 515 382 635 1451 Fragments arranged by size: 1451 1304 1246 1221 936 635 515 382 186 155 83 53 BsaWI w'CCGG_w Cuts at: 2636 3580 6600 6998 7272 7419 7830 2636 Size: 944 3020 398 274 147 411 2973 Fragments arranged by size: 3020 2973 944 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2262 2292 3026 3056 3092 Size: 30 165 30 859 30 734 30 36 Cuts at: 3092 3122 3245 3275 3341 3371 1178 Size: 30 123 30 66 30 5974 Fragments arranged by size: 5974 859 734 165 123 66 36 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2063 2084 2195 2237 2387 3023 3026 3113 Size: 877 21 111 42 150 636 3 87 Cuts at: 3113 3362 3733 3746 4151 4904 5126 5141 5150 Size: 249 371 13 405 753 222 15 9 Cuts at: 5150 5282 5306 5309 1186 Size: 132 24 3 4044 Fragments arranged by size: 4044 877 753 636 405 371 249 222 150 132 111 87 42 24 21 15 13 9 3 3 BseYI C'CCAG_C Cuts at: 2035 2307 4262 7370 7866 2035 Size: 272 1955 3108 496 2336 Fragments arranged by size: 3108 2336 1955 496 272 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2126 2625 3263 3743 4402 4492 4613 Size: 573 390 499 638 480 659 90 121 Cuts at: 4613 4871 1163 Size: 258 4459 Fragments arranged by size: 4459 659 638 573 499 480 390 258 121 90 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5617 6378 7406 1540 Size: 275 186 3616 761 1028 2301 Fragments arranged by size: 3616 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2627 3039 3049 3168 3201 3561 3705 4107 4896 Size: 412 10 119 33 360 144 402 789 Cuts at: 4896 6228 6313 7384 2627 Size: 1332 85 1071 3410 Fragments arranged by size: 3410 1332 1071 789 412 402 360 144 119 85 33 10 BsmI GAATG_Cn' Cuts at: 2565 4730 4954 4978 5678 5771 2565 Size: 2165 224 24 700 93 4961 Fragments arranged by size: 4961 2165 700 224 93 24 BsmBI CGTCTCn'nnnn_ Cuts at: 3425 5450 3425 Size: 2025 6142 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2194 2280 2627 2731 3039 3049 3168 Size: 399 404 86 347 104 308 10 119 Cuts at: 3168 3201 3561 3705 4107 4896 5486 6228 6313 Size: 33 360 144 402 789 590 742 85 Cuts at: 6313 7384 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 789 742 590 404 402 399 360 347 308 144 119 104 86 85 33 10 BspEI T'CCGG_A Cuts at: 3580 7830 3580 Size: 4250 3917 BspHI T'CATG_A Cuts at: 3216 6056 7786 3216 Size: 2840 1730 3597 Fragments arranged by size: 3597 2840 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4060 4490 4531 5522 844 Size: 3216 430 41 991 3489 Fragments arranged by size: 3489 3216 991 430 41 BsrBI CCG'CTC Cuts at: 1394 1823 3493 4141 6054 1394 Size: 429 1670 648 1913 3507 Fragments arranged by size: 3507 1913 1670 648 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3421 4273 6662 6836 65 Size: 1183 2173 852 2389 174 1396 Fragments arranged by size: 2389 2173 1396 1183 852 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3264 4085 6811 6998 1700 Size: 110 1454 821 2726 187 2869 Fragments arranged by size: 2869 2726 1454 821 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8167 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8167 BssSI C'ACGA_G Cuts at: 6227 7239 6227 Size: 1012 7155 BstAPI GCAn_nnn'nTGC Cuts at: 794 2689 3277 3340 4066 4591 4725 794 Size: 1895 588 63 726 525 134 4236 Fragments arranged by size: 4236 1895 726 588 525 134 63 BstBI TT'CG_AA Cuts at: 6971 6971 Size: 8167 BstEII G'GTnAC_C Cuts at: 1184 4999 1184 Size: 3815 4352 BstYI r'GATC_y Cuts at: 1972 2334 2451 2524 2770 3927 5206 5497 5864 Size: 362 117 73 246 1157 1279 291 367 Cuts at: 5864 6249 6266 7718 7804 7816 8162 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1279 1157 385 367 362 346 291 246 117 86 73 17 12 Bsu36I CC'TnA_GG Cuts at: 3383 5211 3383 Size: 1828 6339 BtgI C'CryG_G Cuts at: 513 1065 4740 513 Size: 552 3675 3940 Fragments arranged by size: 3940 3675 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2978 487 Size: 683 56 1752 5676 Fragments arranged by size: 5676 1752 683 56 BtsI GCAGTG_nn' Cuts at: 2125 3362 3617 3701 3770 3776 4883 5180 5683 Size: 1237 255 84 69 6 1107 297 503 Cuts at: 5683 6477 6497 2125 Size: 794 20 3795 Fragments arranged by size: 3795 1237 1107 794 503 297 255 84 69 20 6 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8167 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8132 DraI TTT'AAA Cuts at: 5471 5471 Size: 8167 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7174 809 Size: 507 5858 1802 Fragments arranged by size: 5858 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4659 5614 6503 Size: 54 1425 50 275 18 2829 955 889 Cuts at: 6503 8132 8 Size: 1629 43 Fragments arranged by size: 2829 1629 1425 955 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5614 1537 Size: 275 3802 4090 Fragments arranged by size: 4090 3802 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2049 2055 2061 2073 2615 2919 2973 3747 Size: 696 6 6 12 542 304 54 774 Cuts at: 3747 3930 4139 4235 4403 4445 4484 4677 4907 Size: 183 209 96 168 42 39 193 230 Cuts at: 4907 5244 5259 5283 5317 6097 1353 Size: 337 15 24 34 780 3423 Fragments arranged by size: 3423 780 774 696 542 337 304 230 209 193 183 168 96 54 42 39 34 24 15 12 6 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4886 6754 7128 7274 387 Size: 1064 138 3297 1868 374 146 1280 Fragments arranged by size: 3297 1868 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2625 3037 3166 3199 3559 3703 4105 4894 2625 Size: 412 129 33 360 144 402 789 5898 Fragments arranged by size: 5898 789 412 402 360 144 129 33 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2198 2240 2303 2795 2841 2943 3125 Size: 393 566 42 63 492 46 102 182 Cuts at: 3125 3173 3269 3431 3509 3539 3566 3728 4554 Size: 48 96 162 78 30 27 162 826 Cuts at: 4554 4697 4883 5075 6230 6827 7614 7882 1239 Size: 143 186 192 1155 597 787 268 1524 Fragments arranged by size: 1524 1155 826 787 597 566 492 393 268 192 186 182 162 162 143 102 96 78 63 48 46 42 30 27 EcoNI CCTnn'n_nnAGG Cuts at: 2519 2714 4699 5409 2519 Size: 195 1985 710 5277 Fragments arranged by size: 5277 1985 710 195 EcoO109I rG'GnC_Cy Cuts at: 2728 3526 4063 2728 Size: 798 537 6832 Fragments arranged by size: 6832 798 537 EcoRI G'AATT_C Cuts at: 5476 5854 5476 Size: 378 7789 FspI TGC'GCA Cuts at: 6673 6673 Size: 8167 HaeII r_GCGC'y Cuts at: 1678 2307 4549 7314 1678 Size: 629 2242 2765 2531 Fragments arranged by size: 2765 2531 2242 629 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2145 2177 2443 2475 2495 Size: 32 135 32 573 32 266 32 20 Cuts at: 2495 2527 3092 3124 3424 3456 5294 5326 5447 Size: 32 565 32 300 32 1838 32 121 Cuts at: 5447 5479 6808 6840 6882 6914 1373 Size: 32 1329 32 42 32 2626 Fragments arranged by size: 2626 1838 1329 573 565 300 266 135 121 42 32 32 32 32 32 32 32 32 32 32 32 20 HincII GTy'rAC Cuts at: 669 1657 1938 2140 5511 5757 669 Size: 988 281 202 3371 246 3079 Fragments arranged by size: 3371 3079 988 281 246 202 HindIII A'AGCT_T Cuts at: 748 748 Size: 8167 HpaI GTT'AAC Cuts at: 5757 5757 Size: 8167 KpnI G_GTAC'C Cuts at: 5492 5492 Size: 8167 MfeI C'AATT_G Cuts at: 5766 5766 Size: 8167 MluI A'CGCG_T Cuts at: 8043 8043 Size: 8167 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2955 3957 7281 7465 1305 Size: 1650 1002 3324 184 2007 Fragments arranged by size: 3324 2007 1650 1002 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4661 10 Size: 54 1425 343 2829 3516 Fragments arranged by size: 3516 2829 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2253 2499 3507 6125 6484 6643 518 Size: 648 1087 246 1008 2618 359 159 2042 Fragments arranged by size: 2618 2042 1087 1008 648 359 246 159 MspA1I CmG'CkG Cuts at: 1606 2206 3181 3340 3463 3517 3544 3682 3745 Size: 600 975 159 123 54 27 138 63 Cuts at: 3745 3850 4189 4506 4633 4804 4861 4873 5583 Size: 105 339 317 127 171 57 12 710 Cuts at: 5583 6260 7408 7653 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 975 710 677 600 339 317 245 171 159 138 127 123 105 63 57 54 27 12 NaeI GCC'GGC Cuts at: 3266 3266 Size: 8167 NcoI C'CATG_G Cuts at: 513 1065 4740 513 Size: 552 3675 3940 Fragments arranged by size: 3940 3675 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8167 NgoMIV G'CCGG_C Cuts at: 3264 3264 Size: 8167 NheI G'CTAG_C Cuts at: 1052 2296 4817 1052 Size: 1244 2521 4402 Fragments arranged by size: 4402 2521 1244 NotI GC'GGCC_GC Cuts at: 5614 5614 Size: 8167 NsiI A_TGCA'T Cuts at: 2937 2937 Size: 8167 NspI r_CATG'y Cuts at: 1132 1229 3306 3508 3868 5525 7070 1132 Size: 97 2077 202 360 1657 1545 2229 Fragments arranged by size: 2229 2077 1657 1545 360 202 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8167 PciI A'CATG_T Cuts at: 3302 3504 7066 3302 Size: 202 3562 4403 Fragments arranged by size: 4403 3562 202 PflMI CCAn_nnn'nTGG Cuts at: 1108 3788 1108 Size: 2680 5487 PfoI T'CCnGG_A Cuts at: 2280 3705 3966 2280 Size: 1425 261 6481 Fragments arranged by size: 6481 1425 261 PmeI GTTT'AAAC Cuts at: 5471 5471 Size: 8167 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6236 6268 7749 7781 6236 Size: 32 1481 32 6622 Fragments arranged by size: 6622 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3526 3526 Size: 8167 PshAI GACnn'nnGTC Cuts at: 1613 4086 1613 Size: 2473 5694 PsiI TTA'TAA Cuts at: 5737 5737 Size: 8167 PspOMI G'GGCC_C Cuts at: 2727 2727 Size: 8167 PstI C_TGCA'G Cuts at: 830 2205 2520 2685 2736 2817 3030 3129 3273 Size: 1375 315 165 51 81 213 99 144 Cuts at: 3273 3345 3357 3378 3687 3750 3849 4071 4155 Size: 72 12 21 309 63 99 222 84 Cuts at: 4155 4587 4782 4803 4878 5519 830 Size: 432 195 21 75 641 3478 Fragments arranged by size: 3478 1375 641 432 315 309 222 213 195 165 144 99 99 84 81 75 72 63 51 21 21 12 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8167 PvuII CAG'CTG Cuts at: 2206 3181 3340 3463 3517 3544 3682 3745 3850 Size: 975 159 123 54 27 138 63 105 Cuts at: 3850 4189 4506 4633 4804 4861 4873 2206 Size: 339 317 127 171 57 12 5500 Fragments arranged by size: 5500 975 339 317 171 159 138 127 123 105 63 57 54 27 12 SacI G_AGCT'C Cuts at: 2627 3039 3168 3201 3561 3705 4107 4896 2627 Size: 412 129 33 360 144 402 789 5898 Fragments arranged by size: 5898 789 412 402 360 144 129 33 SalI G'TCGA_C Cuts at: 1936 5509 1936 Size: 3573 4594 SapI GCTCTTCn'nnn_ Cuts at: 2615 2919 2973 3747 3930 4235 4677 2615 Size: 304 54 774 183 305 442 6105 Fragments arranged by size: 6105 774 442 305 304 183 54 SbfI CC_TGCA'GG Cuts at: 2520 2736 4071 5519 2520 Size: 216 1335 1448 5168 Fragments arranged by size: 5168 1448 1335 216 ScaI AGT'ACT Cuts at: 6415 8127 6415 Size: 1712 6455 SfcI C'TryA_G Cuts at: 826 1045 2201 2270 2516 2681 2732 2813 2984 Size: 219 1156 69 246 165 51 81 171 Cuts at: 2984 3026 3125 3269 3341 3353 3374 3683 3746 Size: 42 99 144 72 12 21 309 63 Cuts at: 3746 3845 4067 4151 4583 4778 4799 4874 5515 Size: 99 222 84 432 195 21 75 641 Cuts at: 5515 6650 7331 7522 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1156 1135 681 641 432 309 246 222 219 195 191 171 165 144 99 99 84 81 75 72 69 63 51 42 21 21 12 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8167 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8167 SmaI CCC'GGG Cuts at: 1872 2231 4940 5494 1872 Size: 359 2709 554 4545 Fragments arranged by size: 4545 2709 554 359 SmlI C'TyrA_G Cuts at: 820 1017 1943 2411 3038 3560 3800 4226 4364 Size: 197 926 468 627 522 240 426 138 Cuts at: 4364 5036 6271 7172 7434 820 Size: 672 1235 901 262 1553 Fragments arranged by size: 1553 1235 926 901 672 627 522 468 426 262 240 197 138 SnaBI TAC'GTA Cuts at: 493 5958 493 Size: 5465 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8167 SphI G_CATG'C Cuts at: 1132 3868 5525 1132 Size: 2736 1657 3774 Fragments arranged by size: 3774 2736 1657 SspI AAT'ATT Cuts at: 5 52 6091 5 Size: 47 6039 2081 Fragments arranged by size: 6039 2081 47 StuI AGG'CCT Cuts at: 1517 3566 4400 4918 5415 1517 Size: 2049 834 518 497 4269 Fragments arranged by size: 4269 2049 834 518 497 StyI C'CwwG_G Cuts at: 513 1065 2262 2347 3859 4740 5606 513 Size: 552 1197 85 1512 881 866 3074 Fragments arranged by size: 3074 1512 1197 881 866 552 85 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3104 4355 6194 6211 6364 6549 6994 Size: 1255 1524 1251 1839 17 153 185 445 Cuts at: 6994 325 Size: 1498 Fragments arranged by size: 1839 1524 1498 1255 1251 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3651 5067 6413 Size: 274 80 33 51 1443 1674 1416 1346 Cuts at: 6413 8125 96 Size: 1712 138 Fragments arranged by size: 1712 1674 1443 1416 1346 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3026 3058 3245 3277 3341 3373 8127 8159 3026 Size: 32 187 32 64 32 4754 32 3034 Fragments arranged by size: 4754 3034 187 64 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 5461 1316 Size: 4145 4022 XbaI T'CTAG_A Cuts at: 5503 5503 Size: 8167 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 4045 5050 2011 Size: 2034 1005 5128 Fragments arranged by size: 5128 2034 1005 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8167 XmaI C'CCGG_G Cuts at: 1870 2229 4938 5492 1870 Size: 359 2709 554 4545 Fragments arranged by size: 4545 2709 554 359 XmnI GAAnn'nnTTC Cuts at: 2565 6296 2565 Size: 3731 4436 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2838 5460 5975 276 Size: 53 83 186 936 1304 2622 515 2468 Fragments arranged by size: 2622 2468 1304 936 515 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BbeI BbvCI BsiWI BstXI BstZ17I DraIII EcoRV FalI FseI FspAI KasI NarI NruI PacI PmlI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI