Restriction map for
pFN21AA1788
(Circular) (Six-base) MAPSORT of: pFN21AA1788.seq Check: 4556 from: 1 to: 5938
pFN21AA1788 5938 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3748 278
Size: 53 83 186 936 2212 2468
Fragments arranged by size:
2468 2212 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2288 3281 1937
Size: 351 993 4594
Fragments arranged by size:
4594 993 351
Acc65I G'GTAC_C
Cuts at: 3259 3259
Size: 5938
AclI AA'CG_TT
Cuts at: 1563 4065 4438 1563
Size: 2502 373 3063
Fragments arranged by size:
3063 2502 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4001 5385 5653 1632
Size: 2369 1384 268 1917
Fragments arranged by size:
2369 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5741
AflIII A'CryG_T
Cuts at: 1203 2724 4837 5814 1203
Size: 1521 2113 977 1327
Fragments arranged by size:
2113 1521 1327 977
AgeI A'CCGG_T
Cuts at: 4769 4769
Size: 5938
AhdI GACnn_n'nnGTC
Cuts at: 4667 4667
Size: 5938
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5938
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5938
AlwNI CAG_nnn'CTG
Cuts at: 2221 3681 5253 2221
Size: 1460 1572 2906
Fragments arranged by size:
2906 1572 1460
ApaI G_GGCC'C
Cuts at: 2197 2574 2197
Size: 377 5561
ApaLI G'TGCA_C
Cuts at: 3995 5151 3995
Size: 1156 4782
ApoI r'AATT_y
Cuts at: 1493 3247 3473 3625 5734 1493
Size: 1754 226 152 2109 1697
Fragments arranged by size:
2109 1754 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4492 160
Size: 872 3460 1606
Fragments arranged by size:
3460 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5938
AvaI C'yCGr_G
Cuts at: 1870 1943 2889 3175 3263 1870
Size: 73 946 286 88 4545
Fragments arranged by size:
4545 946 286 88 73
BamHI G'GATC_C
Cuts at: 3268 3635 3268
Size: 367 5571
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2384 2986 3259 4714 618
Size: 325 217 627 597 602 273 1455 1842
Fragments arranged by size:
1842 1455 627 602 597 325 273 217
BanII G_rGCy'C
Cuts at: 1391 2096 2197 2261 2483 2574 3150 3257 1391
Size: 705 101 64 222 91 576 107 4072
Fragments arranged by size:
4072 705 576 222 107 101 91 64
BbeI G_GCGC'C
Cuts at: 2388 2388
Size: 5938
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2692 2714 928
Size: 971 793 22 4152
Fragments arranged by size:
4152 971 793 22
BbvCI CC'TCA_GC
Cuts at: 3122 3122
Size: 5938
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2370 2404 2828 2862 4129 4163 1903
Size: 34 433 34 424 34 1267 34 3678
Fragments arranged by size:
3678 1267 433 424 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3830 5040 3830
Size: 1210 4728
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5938
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3138 4549 136
Size: 107 122 71 631 605 1466 1411 1525
Fragments arranged by size:
1525 1466 1411 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5933 5933
Size: 5938
BlpI GC'TnA_GC
Cuts at: 3355 3355
Size: 5938
Bme1580I G_kGCm'C
Cuts at: 1790 2197 2574 3999 5155 1790
Size: 407 377 1425 1156 2573
Fragments arranged by size:
2573 1425 1156 407 377
BmgBI CAC'GTC
Cuts at: 1318 2725 1318
Size: 1407 4531
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3669 4627 442
Size: 416 53 83 399 24 2252 958 1753
Fragments arranged by size:
2252 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5938
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2255 2405 2702 2711 4598 1239
Size: 1016 150 297 9 1887 2579
Fragments arranged by size:
2579 1887 1016 297 150 9
Bpu10I CC'TnA_GC
Cuts at: 3122 3122
Size: 5938
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4063 4928 5226 4063
Size: 865 298 4775
Fragments arranged by size:
4775 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4601 882
Size: 617 3102 2219
Fragments arranged by size:
3102 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3079 3729 493
Size: 711 1875 650 2702
Fragments arranged by size:
2702 1875 711 650
BsaBI GATnn'nnATC
Cuts at: 5932 5932
Size: 5938
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2058 2181 2385 2396
Size: 53 83 186 936 525 123 204 11
Cuts at: 2396 3745 4127 4762 275
Size: 1349 382 635 1451
Fragments arranged by size:
1451 1349 936 635 525 382 204 186
123 83 53 11
BsaWI w'CCGG_w
Cuts at: 2655 4371 4769 5043 5190 5601 2655
Size: 1716 398 274 147 411 2992
Fragments arranged by size:
2992 1716 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2081 2111 1178
Size: 30 165 30 678 30 5005
Fragments arranged by size:
5005 678 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2699 1186
Size: 1513 4425
BseYI C'CCAG_C
Cuts at: 2207 2246 2280 2301 2346 2558 2600 5141 5637
Size: 39 34 21 45 212 42 2541 496
Cuts at: 5637 2207
Size: 2508
Fragments arranged by size:
2541 2508 496 212 45 42 39 34
21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2798 1163
Size: 573 1062 4303
Fragments arranged by size:
4303 1062 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2046 2154 3388 4149 5177 1540
Size: 275 186 45 108 1234 761 1028 2301
Fragments arranged by size:
2301 1234 1028 761 275 186 108 45
BsiHKAI G_wGCw'C
Cuts at: 2483 3150 3213 3999 4084 5155 2483
Size: 667 63 786 85 1071 3266
Fragments arranged by size:
3266 1071 786 667 85 63
BsmI GAATG_Cn'
Cuts at: 2014 3449 3542 2014
Size: 1435 93 4410
Fragments arranged by size:
4410 1435 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2029 2029
Size: 5938
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2096 2197 2261 2483 2574 3150 3213
Size: 399 306 101 64 222 91 576 63
Cuts at: 3213 3257 3999 4084 5155 1391
Size: 44 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 576 399 306 222 101
91 85 64 63 44
BspEI T'CCGG_A
Cuts at: 5601 5601
Size: 5938
BspHI T'CATG_A
Cuts at: 3827 5557 3827
Size: 1730 4208
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2446 2770 2833 3293 844
Size: 1602 324 63 460 3489
Fragments arranged by size:
3489 1602 460 324 63
BsrBI CCG'CTC
Cuts at: 1394 1823 3825 1394
Size: 429 2002 3507
Fragments arranged by size:
3507 2002 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4433 4607 65
Size: 1183 3185 174 1396
Fragments arranged by size:
3185 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2041 4582 4769 1700
Size: 110 231 2541 187 2869
Fragments arranged by size:
2869 2541 231 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5938
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5938
BssSI C'ACGA_G
Cuts at: 3998 5010 3998
Size: 1012 4926
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5938
BstBI TT'CG_AA
Cuts at: 2848 4742 2848
Size: 1894 4044
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5938
BstXI CCAn_nnnn'nTGG
Cuts at: 2608 3020 2608
Size: 412 5526
BstYI r'GATC_y
Cuts at: 1972 3268 3635 4020 4037 5489 5575 5587 5933
Size: 1296 367 385 17 1452 86 12 346
Cuts at: 5933 1972
Size: 1977
Fragments arranged by size:
1977 1452 1296 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2052 513
Size: 552 987 4399
Fragments arranged by size:
4399 987 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3166 487
Size: 683 56 1940 3259
Fragments arranged by size:
3259 1940 683 56
BtsI GCAGTG_nn'
Cuts at: 2862 3454 4248 4268 2862
Size: 592 794 20 4532
Fragments arranged by size:
4532 794 592 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5938
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5903
DraI TTT'AAA
Cuts at: 3242 3242
Size: 5938
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4945 809
Size: 507 3629 1802
Fragments arranged by size:
3629 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2043 2151 2437
Size: 54 1425 50 275 18 213 108 286
Cuts at: 2437 2745 3219 3385 4274 5903 8
Size: 308 474 166 889 1629 43
Fragments arranged by size:
1629 1425 889 474 308 286 275 213
166 108 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2043 2151 3385 1537
Size: 275 231 108 1234 4090
Fragments arranged by size:
4090 1234 275 231 108
EarI CTCTTCn'nnn_
Cuts at: 1353 3868 1353
Size: 2515 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2665 4525 4899 5045 387
Size: 1064 138 1076 1860 374 146 1280
Fragments arranged by size:
1860 1280 1076 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2481 3148 2481
Size: 667 5271
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2255 2405 2702 2711 4001 4598 5385
Size: 393 623 150 297 9 1290 597 787
Cuts at: 5385 5653 1239
Size: 268 1524
Fragments arranged by size:
1524 1290 787 623 597 393 297 268
150 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2765 2765
Size: 5938
EcoO109I rG'GnC_Cy
Cuts at: 2218 2570 2571 2218
Size: 352 1 5585
Fragments arranged by size:
5585 352 1
EcoRI G'AATT_C
Cuts at: 3247 3625 3247
Size: 378 5560
FspI TGC'GCA
Cuts at: 4444 4444
Size: 5938
HaeII r_GCGC'y
Cuts at: 1678 2208 2388 5085 1678
Size: 530 180 2697 2531
Fragments arranged by size:
2697 2531 530 180
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2081 2113 4579 4611 4653
Size: 32 135 32 509 32 2466 32 42
Cuts at: 4653 4685 1373
Size: 32 2626
Fragments arranged by size:
2626 2466 509 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2289 3282 3528 669
Size: 988 281 351 993 246 3079
Fragments arranged by size:
3079 993 988 351 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5938
HpaI GTT'AAC
Cuts at: 3528 3528
Size: 5938
KasI G'GCGC_C
Cuts at: 2384 2384
Size: 5938
KpnI G_GTAC'C
Cuts at: 3263 3263
Size: 5938
MfeI C'AATT_G
Cuts at: 3537 3537
Size: 5938
MluI A'CGCG_T
Cuts at: 5814 5814
Size: 5938
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5052 5236 1305
Size: 3747 184 2007
Fragments arranged by size:
3747 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2747 10
Size: 54 1425 343 915 3201
Fragments arranged by size:
3201 1425 915 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3896 4255 4414 518
Size: 648 2730 359 159 2042
Fragments arranged by size:
2730 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2048 2436 2680 2743 3354 4031 5179 5424
Size: 442 388 244 63 611 677 1148 245
Cuts at: 5424 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 611 442 388 245 244
63
NaeI GCC'GGC
Cuts at: 2043 2043
Size: 5938
NarI GG'CG_CC
Cuts at: 2385 2385
Size: 5938
NcoI C'CATG_G
Cuts at: 513 1065 2052 513
Size: 552 987 4399
Fragments arranged by size:
4399 987 552
NdeI CA'TA_TG
Cuts at: 387 2869 387
Size: 2482 3456
NgoMIV G'CCGG_C
Cuts at: 2041 2041
Size: 5938
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5938
NotI GC'GGCC_GC
Cuts at: 3385 3385
Size: 5938
NspI r_CATG'y
Cuts at: 1132 1229 3001 3296 4841 1132
Size: 97 1772 295 1545 2229
Fragments arranged by size:
2229 1772 1545 295 97
PasI CC'CwG_GG
Cuts at: 1348 2485 1348
Size: 1137 4801
PciI A'CATG_T
Cuts at: 4837 4837
Size: 5938
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3228 1108
Size: 2120 3818
PfoI T'CCnGG_A
Cuts at: 2116 2421 2116
Size: 305 5633
PmeI GTTT'AAAC
Cuts at: 3242 3242
Size: 5938
PmlI CAC'GTG
Cuts at: 3079 3079
Size: 5938
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4007 4039 5520 5552 4007
Size: 32 1481 32 4393
Fragments arranged by size:
4393 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2218 2218
Size: 5938
PshAI GACnn'nnGTC
Cuts at: 1613 2021 1613
Size: 408 5530
PsiI TTA'TAA
Cuts at: 3508 3508
Size: 5938
PspOMI G'GGCC_C
Cuts at: 2193 2570 2193
Size: 377 5561
PspXI vC'TCGA_Gb
Cuts at: 3175 3175
Size: 5938
PstI C_TGCA'G
Cuts at: 830 3290 830
Size: 2460 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5938
PvuII CAG'CTG
Cuts at: 2680 2743 2680
Size: 63 5875
RsrII CG'GwC_CG
Cuts at: 3163 3163
Size: 5938
SacI G_AGCT'C
Cuts at: 2483 3150 2483
Size: 667 5271
SalI G'TCGA_C
Cuts at: 1936 2287 3280 1936
Size: 351 993 4594
Fragments arranged by size:
4594 993 351
SbfI CC_TGCA'GG
Cuts at: 3290 3290
Size: 5938
ScaI AGT'ACT
Cuts at: 4186 5898 4186
Size: 1712 4226
SfcI C'TryA_G
Cuts at: 826 1045 2065 3286 4421 5102 5293 826
Size: 219 1020 1221 1135 681 191 1471
Fragments arranged by size:
1471 1221 1135 1020 681 219 191
SfoI GGC'GCC
Cuts at: 2386 2386
Size: 5938
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5938
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5938
SmaI CCC'GGG
Cuts at: 1872 3265 1872
Size: 1393 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3175 4042 4943 5205 820
Size: 197 926 1232 867 901 262 1553
Fragments arranged by size:
1553 1232 926 901 867 262 197
SnaBI TAC'GTA
Cuts at: 493 3729 493
Size: 3236 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5938
SphI G_CATG'C
Cuts at: 1132 3296 1132
Size: 2164 3774
SspI AAT'ATT
Cuts at: 5 52 3862 5
Size: 47 3810 2081
Fragments arranged by size:
3810 2081 47
StuI AGG'CCT
Cuts at: 1517 3132 1517
Size: 1615 4323
StyI C'CwwG_G
Cuts at: 513 1065 2052 3204 3377 513
Size: 552 987 1152 173 3074
Fragments arranged by size:
3074 1152 987 552 173
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2477 3965 3982 4135 4320 4765 325
Size: 1255 897 1488 17 153 185 445 1498
Fragments arranged by size:
1498 1488 1255 897 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4184 5896 96
Size: 274 80 33 51 1443 2207 1712 138
Fragments arranged by size:
2207 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5898 5930 5898
Size: 32 5906
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5938
XbaI T'CTAG_A
Cuts at: 3274 3274
Size: 5938
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2677 2677
Size: 5938
XhoI C'TCGA_G
Cuts at: 1943 3175 1943
Size: 1232 4706
XmaI C'CCGG_G
Cuts at: 1870 3263 1870
Size: 1393 4545
XmnI GAAnn'nnTTC
Cuts at: 2699 4067 2699
Size: 1368 4570
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3746 276
Size: 53 83 186 936 2212 2468
Fragments arranged by size:
2468 2212 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII RsrII SacI
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsiWI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI
NruI NsiI PacI PsrI SacII SanDI SapI SexAI
SfiI SrfI SwaI