(Circular) (Six-base) MAPSORT of: pFN21AA1714.seq Check: 9568 from: 1 to: 8086 pFN21AA1714 8086 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3326 4287 3326 Size: 961 7125 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5896 278 Size: 53 83 186 936 4360 2468 Fragments arranged by size: 4360 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5429 1937 Size: 3492 4594 Acc65I G'GTAC_C Cuts at: 5407 5407 Size: 8086 AclI AA'CG_TT Cuts at: 1563 5385 6213 6586 1563 Size: 3822 828 373 3063 Fragments arranged by size: 3822 3063 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 1995 2119 6149 7533 7801 1632 Size: 363 124 4030 1384 268 1917 Fragments arranged by size: 4030 1917 1384 363 268 124 AfeI AGC'GCT Cuts at: 4028 4028 Size: 8086 AflII C'TTAA_G Cuts at: 820 1017 3272 820 Size: 197 2255 5634 Fragments arranged by size: 5634 2255 197 AflIII A'CryG_T Cuts at: 1203 4452 6985 7962 1203 Size: 3249 2533 977 1327 Fragments arranged by size: 3249 2533 1327 977 AgeI A'CCGG_T Cuts at: 6917 6917 Size: 8086 AhdI GACnn_n'nnGTC Cuts at: 3178 6815 3178 Size: 3637 4449 AleI CACnn'nnGTG Cuts at: 1166 4550 1166 Size: 3384 4702 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4903 1184 Size: 3719 4367 AlwNI CAG_nnn'CTG Cuts at: 3341 3436 3669 4042 5829 7401 3341 Size: 95 233 373 1787 1572 4026 Fragments arranged by size: 4026 1787 1572 373 233 95 ApaLI G'TGCA_C Cuts at: 2429 6143 7299 2429 Size: 3714 1156 3216 Fragments arranged by size: 3714 3216 1156 ApoI r'AATT_y Cuts at: 1493 2636 2661 2946 4381 5395 5621 5773 7882 Size: 1143 25 285 1435 1014 226 152 2109 Cuts at: 7882 1493 Size: 1697 Fragments arranged by size: 2109 1697 1435 1143 1014 285 226 152 25 AseI AT'TA_AT Cuts at: 160 1032 2711 6640 160 Size: 872 1679 3929 1606 Fragments arranged by size: 3929 1679 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8086 AvaI C'yCGr_G Cuts at: 1870 1943 2170 5411 1870 Size: 73 227 3241 4545 Fragments arranged by size: 4545 3241 227 73 AvrII C'CTAG_G Cuts at: 2123 2495 2123 Size: 372 7714 BamHI G'GATC_C Cuts at: 3577 5416 5783 3577 Size: 1839 367 5880 Fragments arranged by size: 5880 1839 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3938 3951 4068 4632 5407 Size: 325 217 627 2151 13 117 564 775 Cuts at: 5407 6862 618 Size: 1455 1842 Fragments arranged by size: 2151 1842 1455 775 627 564 325 217 117 13 BanII G_rGCy'C Cuts at: 1391 2069 2312 2841 3134 5183 5405 1391 Size: 678 243 529 293 2049 222 4072 Fragments arranged by size: 4072 2049 678 529 293 243 222 BbeI G_GCGC'C Cuts at: 4072 4072 Size: 8086 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 7115 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5149 5183 6277 6311 1903 Size: 34 3212 34 1094 34 3678 Fragments arranged by size: 3678 3212 1094 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5978 7188 5978 Size: 1210 6876 BclI T'GATC_A Cuts at: 1548 4681 5127 1548 Size: 3133 446 4507 Fragments arranged by size: 4507 3133 446 BfrBI ATG'CAT Cuts at: 4797 5273 4797 Size: 476 7610 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6697 136 Size: 107 122 71 631 605 5025 1525 Fragments arranged by size: 5025 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 8081 8081 Size: 8086 BlpI GC'TnA_GC Cuts at: 2754 5503 2754 Size: 2749 5337 Bme1580I G_kGCm'C Cuts at: 1790 2433 3943 3954 4725 6147 7303 1790 Size: 643 1510 11 771 1422 1156 2573 Fragments arranged by size: 2573 1510 1422 1156 771 643 11 BmgBI CAC'GTC Cuts at: 1318 3245 4453 1318 Size: 1927 1208 4951 Fragments arranged by size: 4951 1927 1208 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2326 3688 4147 Size: 416 53 83 399 24 909 1362 459 Cuts at: 4147 4352 5817 6775 442 Size: 205 1465 958 1753 Fragments arranged by size: 1753 1465 1362 958 909 459 416 399 205 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8086 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2426 3118 3608 5059 6746 1239 Size: 1187 692 490 1451 1687 2579 Fragments arranged by size: 2579 1687 1451 1187 692 490 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2015 6211 7076 7374 2015 Size: 4196 865 298 2727 Fragments arranged by size: 4196 2727 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3740 4809 6749 882 Size: 617 2241 1069 1940 2219 Fragments arranged by size: 2241 2219 1940 1069 617 BsaAI yAC'GTr Cuts at: 493 1204 4639 5877 493 Size: 711 3435 1238 2702 Fragments arranged by size: 3435 2702 1238 711 BsaBI GATnn'nnATC Cuts at: 2516 5256 8080 2516 Size: 2740 2824 2522 Fragments arranged by size: 2824 2740 2522 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3885 4069 4856 5893 Size: 53 83 186 936 2352 184 787 1037 Cuts at: 5893 6275 6910 275 Size: 382 635 1451 Fragments arranged by size: 2352 1451 1037 936 787 635 382 186 184 83 53 BsaWI w'CCGG_w Cuts at: 6519 6917 7191 7338 7749 6519 Size: 398 274 147 411 6856 Fragments arranged by size: 6856 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2758 2788 3015 3045 3695 Size: 30 165 30 1355 30 227 30 650 Cuts at: 3695 3725 4859 4889 5140 5170 1178 Size: 30 1134 30 251 30 4094 Fragments arranged by size: 4094 1355 1134 650 251 227 165 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2134 2831 5017 1186 Size: 948 697 2186 4255 Fragments arranged by size: 4255 2186 948 697 BseYI C'CCAG_C Cuts at: 3604 3662 3944 7289 7785 3604 Size: 58 282 3345 496 3905 Fragments arranged by size: 3905 3345 496 282 58 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3923 4619 4899 1163 Size: 573 2187 696 280 4350 Fragments arranged by size: 4350 2187 696 573 280 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5536 6297 7325 1540 Size: 275 186 3535 761 1028 2301 Fragments arranged by size: 3535 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2312 2433 2578 2841 3134 3450 4278 5183 6147 Size: 121 145 263 293 316 828 905 964 Cuts at: 6147 6232 7303 2312 Size: 85 1071 3095 Fragments arranged by size: 3095 1071 964 905 828 316 293 263 145 121 85 BsiWI C'GTAC_G Cuts at: 3978 3978 Size: 8086 BsmI GAATG_Cn' Cuts at: 2821 2972 4797 5597 5690 2821 Size: 151 1825 800 93 5217 Fragments arranged by size: 5217 1825 800 151 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2613 4674 2613 Size: 2061 6025 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2069 2312 2433 2578 2841 3134 3450 Size: 399 279 243 121 145 263 293 316 Cuts at: 3450 3943 3954 4278 4725 5183 5405 6147 6232 Size: 493 11 324 447 458 222 742 85 Cuts at: 6232 7303 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 493 458 447 399 324 316 293 279 263 243 222 145 121 85 11 BspEI T'CCGG_A Cuts at: 7749 7749 Size: 8086 BspHI T'CATG_A Cuts at: 3069 5975 7705 3069 Size: 2906 1730 3450 Fragments arranged by size: 3450 2906 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2842 3326 3758 4287 5441 844 Size: 1998 484 432 529 1154 3489 Fragments arranged by size: 3489 1998 1154 529 484 432 BsrBI CCG'CTC Cuts at: 1394 1823 5973 1394 Size: 429 4150 3507 Fragments arranged by size: 4150 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2662 2755 4921 6581 6755 65 Size: 1183 1414 93 2166 1660 174 1396 Fragments arranged by size: 2166 1660 1414 1396 1183 174 93 BsrFI r'CCGG_y Cuts at: 1700 1810 6730 6917 1700 Size: 110 4920 187 2869 Fragments arranged by size: 4920 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8086 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8086 BssSI C'ACGA_G Cuts at: 4080 6146 7158 4080 Size: 2066 1012 5008 Fragments arranged by size: 5008 2066 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 5015 794 Size: 4221 3865 BstBI TT'CG_AA Cuts at: 3199 6890 3199 Size: 3691 4395 BstEII G'GTnAC_C Cuts at: 1184 2320 3925 4948 1184 Size: 1136 1605 1023 4322 Fragments arranged by size: 4322 1605 1136 1023 BstXI CCAn_nnnn'nTGG Cuts at: 2914 4101 2914 Size: 1187 6899 BstYI r'GATC_y Cuts at: 1972 2917 3063 3577 4831 5416 5783 6168 6185 Size: 945 146 514 1254 585 367 385 17 Cuts at: 6185 7637 7723 7735 8081 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1254 945 585 514 385 367 346 146 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3545 3545 Size: 8086 BtgI C'CryG_G Cuts at: 513 1065 2004 2164 2865 4008 4360 4432 513 Size: 552 939 160 701 1143 352 72 4167 Fragments arranged by size: 4167 1143 939 701 552 352 160 72 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7347 Fragments arranged by size: 7347 683 56 BtsI GCAGTG_nn' Cuts at: 2277 3365 4349 4443 4885 5602 6396 6416 2277 Size: 1088 984 94 442 717 794 20 3947 Fragments arranged by size: 3947 1088 984 794 717 442 94 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8086 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8051 DraI TTT'AAA Cuts at: 2607 2641 5290 5390 2607 Size: 34 2649 100 5303 Fragments arranged by size: 5303 2649 100 34 DraIII CAC_nnn'GTG Cuts at: 3370 4639 5320 3370 Size: 1269 681 6136 Fragments arranged by size: 6136 1269 681 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2852 4256 4908 7093 809 Size: 507 1536 1404 652 2185 1802 Fragments arranged by size: 2185 1802 1536 1404 652 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4228 4767 5533 Size: 54 1425 50 275 18 2398 539 766 Cuts at: 5533 6422 8051 8 Size: 889 1629 43 Fragments arranged by size: 2398 1629 1425 889 766 539 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5533 1537 Size: 275 3721 4090 Fragments arranged by size: 4090 3721 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2188 3305 4677 4757 6016 1353 Size: 835 1117 1372 80 1259 3423 Fragments arranged by size: 3423 1372 1259 1117 835 80 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4037 6673 7047 7193 387 Size: 1064 138 2448 2636 374 146 1280 Fragments arranged by size: 2636 2448 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2310 2839 3132 5181 2310 Size: 529 293 2049 5215 Fragments arranged by size: 5215 2049 529 293 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 1995 2119 2426 3118 3608 5059 6149 Size: 393 363 124 307 692 490 1451 1090 Cuts at: 6149 6746 7533 7801 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1451 1090 787 692 597 490 393 363 307 268 124 EcoNI CCTnn'n_nnAGG Cuts at: 2122 2122 Size: 8086 EcoO109I rG'GnC_Cy Cuts at: 3619 3619 Size: 8086 EcoRI G'AATT_C Cuts at: 2661 2946 4381 5395 5773 2661 Size: 285 1435 1014 378 4974 Fragments arranged by size: 4974 1435 1014 378 285 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2741 2746 2741 Size: 5 8081 FspI TGC'GCA Cuts at: 6592 6592 Size: 8086 HaeII r_GCGC'y Cuts at: 1678 3605 4030 4072 7233 1678 Size: 1927 425 42 3161 2531 Fragments arranged by size: 3161 2531 1927 425 42 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3164 3196 3482 3514 3695 Size: 32 135 32 1592 32 286 32 181 Cuts at: 3695 3727 5112 5144 6727 6759 6801 6833 1373 Size: 32 1385 32 1583 32 42 32 2626 Fragments arranged by size: 2626 1592 1583 1385 286 181 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2857 5430 5676 669 Size: 988 281 919 2573 246 3079 Fragments arranged by size: 3079 2573 988 919 281 246 HindIII A'AGCT_T Cuts at: 748 2723 2986 748 Size: 1975 263 5848 Fragments arranged by size: 5848 1975 263 HpaI GTT'AAC Cuts at: 5676 5676 Size: 8086 KasI G'GCGC_C Cuts at: 4068 4068 Size: 8086 KpnI G_GTAC'C Cuts at: 5411 5411 Size: 8086 MfeI C'AATT_G Cuts at: 5685 5685 Size: 8086 MluI A'CGCG_T Cuts at: 7962 7962 Size: 8086 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2975 4090 7200 7384 1305 Size: 1670 1115 3110 184 2007 Fragments arranged by size: 3110 2007 1670 1115 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 6264 Fragments arranged by size: 6264 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3399 4550 5103 6044 6403 6562 518 Size: 648 2233 1151 553 941 359 159 2042 Fragments arranged by size: 2233 2042 1151 941 648 553 359 159 MspA1I CmG'CkG Cuts at: 1606 3425 3948 3986 4000 4010 4039 4362 5502 Size: 1819 523 38 14 10 29 323 1140 Cuts at: 5502 6179 7327 7572 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1819 1148 1140 677 523 323 245 38 29 14 10 NarI GG'CG_CC Cuts at: 4069 4069 Size: 8086 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 6595 Fragments arranged by size: 6595 939 552 NdeI CA'TA_TG Cuts at: 387 3396 387 Size: 3009 5077 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8086 NotI GC'GGCC_GC Cuts at: 5533 5533 Size: 8086 NsiI A_TGCA'T Cuts at: 4799 5275 4799 Size: 476 7610 NspI r_CATG'y Cuts at: 1132 1229 4541 5444 6989 1132 Size: 97 3312 903 1545 2229 Fragments arranged by size: 3312 2229 1545 903 97 PacI TTA_AT'TAA Cuts at: 2715 2715 Size: 8086 PasI CC'CwG_GG Cuts at: 1348 2791 3523 3847 1348 Size: 1443 732 324 5587 Fragments arranged by size: 5587 1443 732 324 PciI A'CATG_T Cuts at: 6985 6985 Size: 8086 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 8086 PfoI T'CCnGG_A Cuts at: 2401 3134 2401 Size: 733 7353 PmeI GTTT'AAAC Cuts at: 5390 5390 Size: 8086 PmlI CAC'GTG Cuts at: 4639 4639 Size: 8086 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6155 6187 7668 7700 6155 Size: 32 1481 32 6541 Fragments arranged by size: 6541 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3619 3619 Size: 8086 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8086 PsiI TTA'TAA Cuts at: 3018 5656 3018 Size: 2638 5448 PstI C_TGCA'G Cuts at: 830 4812 4892 5438 830 Size: 3982 80 546 3478 Fragments arranged by size: 3982 3478 546 80 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8086 PvuII CAG'CTG Cuts at: 3425 4000 3425 Size: 575 7511 RsrII CG'GwC_CG Cuts at: 4005 4005 Size: 8086 SacI G_AGCT'C Cuts at: 2312 2841 3134 5183 2312 Size: 529 293 2049 5215 Fragments arranged by size: 5215 2049 529 293 SacII CC_GC'GG Cuts at: 4011 4363 4011 Size: 352 7734 SalI G'TCGA_C Cuts at: 1936 5428 1936 Size: 3492 4594 SapI GCTCTTCn'nnn_ Cuts at: 4757 4757 Size: 8086 SbfI CC_TGCA'GG Cuts at: 5438 5438 Size: 8086 ScaI AGT'ACT Cuts at: 6334 8046 6334 Size: 1712 6374 SfcI C'TryA_G Cuts at: 826 1045 2434 4808 4888 5434 6569 7250 7441 Size: 219 1389 2374 80 546 1135 681 191 Cuts at: 7441 826 Size: 1471 Fragments arranged by size: 2374 1471 1389 1135 681 546 219 191 80 SfoI GGC'GCC Cuts at: 4070 4070 Size: 8086 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8086 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8086 SmaI CCC'GGG Cuts at: 1872 2172 5413 1872 Size: 300 3241 4545 Fragments arranged by size: 4545 3241 300 SmlI C'TyrA_G Cuts at: 820 1017 1943 2030 3272 6190 7091 7353 820 Size: 197 926 87 1242 2918 901 262 1553 Fragments arranged by size: 2918 1553 1242 926 901 262 197 87 SnaBI TAC'GTA Cuts at: 493 5877 493 Size: 5384 2702 SpeI A'CTAG_T Cuts at: 152 4244 152 Size: 4092 3994 SphI G_CATG'C Cuts at: 1132 4541 5444 1132 Size: 3409 903 3774 Fragments arranged by size: 3774 3409 903 SspI AAT'ATT Cuts at: 5 52 6010 5 Size: 47 5958 2081 Fragments arranged by size: 5958 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8086 StyI C'CwwG_G Cuts at: 513 1065 2004 2123 2257 2293 2495 3320 5525 Size: 552 939 119 134 36 202 825 2205 Cuts at: 5525 513 Size: 3074 Fragments arranged by size: 3074 2205 939 825 552 202 134 119 36 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2306 2308 2985 4621 4791 6113 6130 Size: 1255 726 2 677 1636 170 1322 17 Cuts at: 6130 6283 6468 6913 325 Size: 153 185 445 1498 Fragments arranged by size: 1636 1498 1322 1255 726 677 445 185 170 153 17 2 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3589 3864 4169 Size: 274 80 33 51 1443 1612 275 305 Cuts at: 4169 6332 8044 96 Size: 2163 1712 138 Fragments arranged by size: 2163 1712 1612 1443 305 275 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2758 2790 2807 2839 3096 3128 3356 3388 4859 Size: 32 17 32 257 32 228 32 1471 Cuts at: 4859 4891 5124 5140 5156 5172 8046 8078 2758 Size: 32 233 16 16 16 2874 32 2766 Fragments arranged by size: 2874 2766 1471 257 233 228 32 32 32 32 32 32 17 16 16 16 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8086 XbaI T'CTAG_A Cuts at: 5422 5422 Size: 8086 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2300 3142 4101 4754 2011 Size: 289 842 959 653 5343 Fragments arranged by size: 5343 959 842 653 289 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8086 XmaI C'CCGG_G Cuts at: 1870 2170 5411 1870 Size: 300 3241 4545 Fragments arranged by size: 4545 3241 300 XmnI GAAnn'nnTTC Cuts at: 6215 6215 Size: 8086 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5894 276 Size: 53 83 186 936 4360 2468 Fragments arranged by size: 4360 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI BaeI BbvCI BplI Bpu10I BstZ17I EcoRV FseI FspAI NaeI NgoMIV NruI PspOMI PspXI PsrI SanDI SexAI SfiI SrfI SwaI