(Circular) (Six-base) MAPSORT of: pFN21AA1687.seq Check: 9166 from: 1 to: 6859 pFN21AA1687 6859 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4669 278 Size: 53 83 186 936 3133 2468 Fragments arranged by size: 3133 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4202 1937 Size: 2265 4594 Acc65I G'GTAC_C Cuts at: 4180 4180 Size: 6859 AclI AA'CG_TT Cuts at: 1563 4986 5359 1563 Size: 3423 373 3063 Fragments arranged by size: 3423 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2096 2210 2466 2490 4922 6306 6574 1632 Size: 464 114 256 24 2432 1384 268 1917 Fragments arranged by size: 2432 1917 1384 464 268 256 114 24 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6662 AflIII A'CryG_T Cuts at: 1203 5758 6735 1203 Size: 4555 977 1327 Fragments arranged by size: 4555 1327 977 AgeI A'CCGG_T Cuts at: 5690 5690 Size: 6859 AhdI GACnn_n'nnGTC Cuts at: 5588 5588 Size: 6859 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6859 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6859 AlwNI CAG_nnn'CTG Cuts at: 2015 2615 3145 4602 6174 2015 Size: 600 530 1457 1572 2700 Fragments arranged by size: 2700 1572 1457 600 530 ApaLI G'TGCA_C Cuts at: 4916 6072 4916 Size: 1156 5703 ApoI r'AATT_y Cuts at: 1493 2272 2344 2704 2960 2985 4168 4394 4546 Size: 779 72 360 256 25 1183 226 152 Cuts at: 4546 6655 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1183 779 360 256 226 152 72 25 AseI AT'TA_AT Cuts at: 160 1032 3015 5413 160 Size: 872 1983 2398 1606 Fragments arranged by size: 2398 1983 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6859 AvaI C'yCGr_G Cuts at: 1870 1943 2172 3981 4184 1870 Size: 73 229 1809 203 4545 Fragments arranged by size: 4545 1809 229 203 73 BamHI G'GATC_C Cuts at: 4189 4556 4189 Size: 367 6492 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2227 3383 3964 4180 5635 Size: 325 217 627 440 1156 581 216 1455 Cuts at: 5635 618 Size: 1842 Fragments arranged by size: 1842 1455 1156 627 581 440 325 217 216 BanII G_rGCy'C Cuts at: 1391 4178 1391 Size: 2787 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2102 2454 2777 928 Size: 971 203 352 323 5010 Fragments arranged by size: 5010 971 352 323 203 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2492 2526 3242 3276 5050 5084 1903 Size: 34 555 34 716 34 1774 34 3678 Fragments arranged by size: 3678 1774 716 555 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4751 5961 4751 Size: 1210 5649 BclI T'GATC_A Cuts at: 1548 1548 Size: 6859 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5470 136 Size: 107 122 71 631 605 3798 1525 Fragments arranged by size: 3798 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2465 3151 6854 2465 Size: 686 3703 2470 Fragments arranged by size: 3703 2470 686 BlpI GC'TnA_GC Cuts at: 4276 4276 Size: 6859 Bme1580I G_kGCm'C Cuts at: 1790 3295 4004 4920 6076 1790 Size: 1505 709 916 1156 2573 Fragments arranged by size: 2573 1505 1156 916 709 BmgBI CAC'GTC Cuts at: 1318 3737 3824 1318 Size: 2419 87 4353 Fragments arranged by size: 4353 2419 87 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3104 4590 5548 Size: 416 53 83 399 24 1687 1486 958 Cuts at: 5548 442 Size: 1753 Fragments arranged by size: 1753 1687 1486 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6859 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2187 3996 4034 2187 Size: 1809 38 5012 Fragments arranged by size: 5012 1809 38 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2080 3889 5519 1239 Size: 841 1809 1630 2579 Fragments arranged by size: 2579 1809 1630 841 Bpu10I CC'TnA_GC Cuts at: 2857 3550 2857 Size: 693 6166 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3206 4984 5849 6147 3206 Size: 1778 865 298 3918 Fragments arranged by size: 3918 1778 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3026 5522 882 Size: 617 1527 2496 2219 Fragments arranged by size: 2496 2219 1527 617 BsaAI yAC'GTr Cuts at: 493 1204 3661 4024 4650 493 Size: 711 2457 363 626 2702 Fragments arranged by size: 2702 2457 711 626 363 BsaBI GATnn'nnATC Cuts at: 6853 6853 Size: 6859 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4666 5048 5683 275 Size: 53 83 186 936 3133 382 635 1451 Fragments arranged by size: 3133 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5292 5690 5964 6111 6522 5292 Size: 398 274 147 411 5629 Fragments arranged by size: 5629 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3405 3435 3787 3817 3887 Size: 30 165 30 2002 30 352 30 70 Cuts at: 3887 3917 1178 Size: 30 4120 Fragments arranged by size: 4120 2002 352 165 70 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6859 BseYI C'CCAG_C Cuts at: 2584 6062 6558 2584 Size: 3478 496 2885 Fragments arranged by size: 3478 2885 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2735 3022 3095 1163 Size: 573 999 287 73 4927 Fragments arranged by size: 4927 999 573 287 73 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4309 5070 6098 1540 Size: 275 186 2308 761 1028 2301 Fragments arranged by size: 2308 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3808 4920 5005 6076 3808 Size: 1112 85 1071 4591 Fragments arranged by size: 4591 1112 1071 85 BsmI GAATG_Cn' Cuts at: 2461 4370 4463 2461 Size: 1909 93 4857 Fragments arranged by size: 4857 1909 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3450 3450 Size: 6859 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3295 3808 4004 4178 4920 5005 6076 Size: 399 1505 513 196 174 742 85 1071 Cuts at: 6076 1391 Size: 2174 Fragments arranged by size: 2174 1505 1071 742 513 399 196 174 85 BspEI T'CCGG_A Cuts at: 6522 6522 Size: 6859 BspHI T'CATG_A Cuts at: 3595 4748 6478 3595 Size: 1153 1730 3976 Fragments arranged by size: 3976 1730 1153 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2145 4214 844 Size: 1301 2069 3489 Fragments arranged by size: 3489 2069 1301 BsrBI CCG'CTC Cuts at: 1394 1823 4746 1394 Size: 429 2923 3507 Fragments arranged by size: 3507 2923 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2637 3898 5354 5528 65 Size: 1183 1389 1261 1456 174 1396 Fragments arranged by size: 1456 1396 1389 1261 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2242 5503 5690 1700 Size: 110 432 3261 187 2869 Fragments arranged by size: 3261 2869 432 187 110 BsrGI T'GTAC_A Cuts at: 96 2891 96 Size: 2795 4064 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6859 BssSI C'ACGA_G Cuts at: 4919 5931 4919 Size: 1012 5847 BstAPI GCAn_nnn'nTGC Cuts at: 794 3073 3299 794 Size: 2279 226 4354 Fragments arranged by size: 4354 2279 226 BstBI TT'CG_AA Cuts at: 5663 5663 Size: 6859 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6859 BstYI r'GATC_y Cuts at: 1972 2043 2465 2689 3151 3242 4189 4556 4941 Size: 71 422 224 462 91 947 367 385 Cuts at: 4941 4958 6410 6496 6508 6854 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 947 462 422 385 367 346 224 91 86 71 17 12 Bsu36I CC'TnA_GG Cuts at: 2048 3356 3639 2048 Size: 1308 283 5268 Fragments arranged by size: 5268 1308 283 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6307 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6120 Fragments arranged by size: 6120 683 56 BtsI GCAGTG_nn' Cuts at: 2616 3010 3062 4375 5169 5189 2616 Size: 394 52 1313 794 20 4286 Fragments arranged by size: 4286 1313 794 394 52 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6859 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3805 3840 529 Size: 35 3241 35 3548 Fragments arranged by size: 3548 3241 35 35 DraI TTT'AAA Cuts at: 3465 4163 3465 Size: 698 6161 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5866 809 Size: 507 4550 1802 Fragments arranged by size: 4550 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3394 4306 5195 Size: 54 1425 50 275 18 1564 912 889 Cuts at: 5195 6824 8 Size: 1629 43 Fragments arranged by size: 1629 1564 1425 912 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4306 1537 Size: 275 2494 4090 Fragments arranged by size: 4090 2494 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2156 2466 2514 2661 3024 3448 4117 4789 Size: 803 310 48 147 363 424 669 672 Cuts at: 4789 1353 Size: 3423 Fragments arranged by size: 3423 803 672 669 424 363 310 147 48 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5446 5820 5966 387 Size: 1064 138 3857 374 146 1280 Fragments arranged by size: 3857 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2080 2096 2210 2466 2490 3889 4922 Size: 393 448 16 114 256 24 1399 1033 Cuts at: 4922 5519 6306 6574 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1399 1033 787 597 448 393 268 256 114 24 16 EcoNI CCTnn'n_nnAGG Cuts at: 2082 2082 Size: 6859 EcoO109I rG'GnC_Cy Cuts at: 2139 3634 2139 Size: 1495 5364 EcoRI G'AATT_C Cuts at: 2960 4168 4546 2960 Size: 1208 378 5273 Fragments arranged by size: 5273 1208 378 FspI TGC'GCA Cuts at: 5365 5365 Size: 6859 HaeII r_GCGC'y Cuts at: 1678 6006 1678 Size: 4328 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2801 2833 3227 3259 3938 Size: 32 135 32 1229 32 394 32 679 Cuts at: 3938 3970 5500 5532 5574 5606 1373 Size: 32 1530 32 42 32 2626 Fragments arranged by size: 2626 1530 1229 679 394 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3534 3675 3957 4114 4203 4449 Size: 988 281 1596 141 282 157 89 246 Cuts at: 4449 669 Size: 3079 Fragments arranged by size: 3079 1596 988 282 281 246 157 141 89 HindIII A'AGCT_T Cuts at: 748 2358 748 Size: 1610 5249 HpaI GTT'AAC Cuts at: 4114 4449 4114 Size: 335 6524 KpnI G_GTAC'C Cuts at: 4184 4184 Size: 6859 MfeI C'AATT_G Cuts at: 2741 4458 2741 Size: 1717 5142 MluI A'CGCG_T Cuts at: 6735 6735 Size: 6859 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3918 4009 5973 6157 1305 Size: 2613 91 1964 184 2007 Fragments arranged by size: 2613 2007 1964 184 91 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5037 Fragments arranged by size: 5037 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 3017 4817 5176 5335 518 Size: 648 843 1008 1800 359 159 2042 Fragments arranged by size: 2042 1800 1008 843 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2794 4275 4952 6100 6345 1606 Size: 1188 1481 677 1148 245 2120 Fragments arranged by size: 2120 1481 1188 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6307 NdeI CA'TA_TG Cuts at: 387 4072 387 Size: 3685 3174 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6859 NotI GC'GGCC_GC Cuts at: 4306 4306 Size: 6859 NspI r_CATG'y Cuts at: 1132 1229 4137 4217 5762 1132 Size: 97 2908 80 1545 2229 Fragments arranged by size: 2908 2229 1545 97 80 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6859 PciI A'CATG_T Cuts at: 5758 5758 Size: 6859 PflMI CCAn_nnn'nTGG Cuts at: 1108 2596 3369 1108 Size: 1488 773 4598 Fragments arranged by size: 4598 1488 773 PmeI GTTT'AAAC Cuts at: 4163 4163 Size: 6859 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4928 4960 6441 6473 4928 Size: 32 1481 32 5314 Fragments arranged by size: 5314 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2139 3634 2139 Size: 1495 5364 PshAI GACnn'nnGTC Cuts at: 1613 2801 1613 Size: 1188 5671 PsiI TTA'TAA Cuts at: 2489 4429 2489 Size: 1940 4919 PstI C_TGCA'G Cuts at: 830 2779 2793 4211 830 Size: 1949 14 1418 3478 Fragments arranged by size: 3478 1949 1418 14 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6859 PvuII CAG'CTG Cuts at: 2794 2794 Size: 6859 SalI G'TCGA_C Cuts at: 1936 4201 1936 Size: 2265 4594 SanDI GG'GwC_CC Cuts at: 2139 3634 2139 Size: 1495 5364 SapI GCTCTTCn'nnn_ Cuts at: 2156 2466 4117 2156 Size: 310 1651 4898 Fragments arranged by size: 4898 1651 310 SbfI CC_TGCA'GG Cuts at: 4211 4211 Size: 6859 ScaI AGT'ACT Cuts at: 5107 6819 5107 Size: 1712 5147 SfcI C'TryA_G Cuts at: 826 1045 2418 2775 2789 3303 3585 3621 4207 Size: 219 1373 357 14 514 282 36 586 Cuts at: 4207 5342 6023 6214 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1373 1135 681 586 514 357 282 219 191 36 14 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6859 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6859 SmaI CCC'GGG Cuts at: 1872 4186 1872 Size: 2314 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3185 3981 4963 5864 6126 820 Size: 197 926 1242 796 982 901 262 1553 Fragments arranged by size: 1553 1242 982 926 901 796 262 197 SnaBI TAC'GTA Cuts at: 493 3661 4650 493 Size: 3168 989 2702 Fragments arranged by size: 3168 2702 989 SpeI A'CTAG_T Cuts at: 152 152 Size: 6859 SphI G_CATG'C Cuts at: 1132 4217 1132 Size: 3085 3774 SspI AAT'ATT Cuts at: 5 52 4783 5 Size: 47 4731 2081 Fragments arranged by size: 4731 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6859 StyI C'CwwG_G Cuts at: 513 1065 2709 2884 4298 513 Size: 552 1644 175 1414 3074 Fragments arranged by size: 3074 1644 1414 552 175 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3049 4886 4903 5056 5241 5686 325 Size: 1255 1469 1837 17 153 185 445 1498 Fragments arranged by size: 1837 1498 1469 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2891 5105 6817 Size: 274 80 33 51 1443 914 2214 1712 Cuts at: 6817 96 Size: 138 Fragments arranged by size: 2214 1712 1443 914 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3787 3819 6819 6851 3787 Size: 32 3000 32 3795 Fragments arranged by size: 3795 3000 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6859 XbaI T'CTAG_A Cuts at: 4195 4195 Size: 6859 XhoI C'TCGA_G Cuts at: 1943 3981 1943 Size: 2038 4821 XmaI C'CCGG_G Cuts at: 1870 4184 1870 Size: 2314 4545 XmnI GAAnn'nnTTC Cuts at: 2348 4988 2348 Size: 2640 4219 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4667 276 Size: 53 83 186 936 3133 2468 Fragments arranged by size: 3133 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BstXI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SexAI SfiI SfoI SrfI SwaI XcmI