Restriction map for
pFN21AA1687
(Linear) (Six-base) MAPSORT of: KIAA1687.seq Check: 2777 from: 1 to: 2154
KIAA1687.orf
With 182 enzymes: SgfI *
February 28, 2007 11:22 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 205 461 485 2154
Size: 91 114 256 24 1669
Fragments arranged by size:
1669 256 114 91 24
AlwNI CAG_nnn'CTG
Cuts at: 0 10 610 1140 2154
Size: 10 600 530 1014
Fragments arranged by size:
1014 600 530 10
ApoI r'AATT_y
Cuts at: 0 267 339 699 955 980 2154
Size: 267 72 360 256 25 1174
Fragments arranged by size:
1174 360 267 256 72 25
AseI AT'TA_AT
Cuts at: 0 1010 2154
Size: 1010 1144
AvaI C'yCGr_G
Cuts at: 0 167 1976 2154
Size: 167 1809 178
Fragments arranged by size:
1809 178 167
BanI G'GyrC_C
Cuts at: 0 222 1378 1959 2154
Size: 222 1156 581 195
Fragments arranged by size:
1156 581 222 195
BbsI GAAGACnn'nnnn_
Cuts at: 0 97 449 772 2154
Size: 97 352 323 1382
Fragments arranged by size:
1382 352 323 97
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 487 521 1237 1271 2154
Size: 487 34 716 34 883
Fragments arranged by size:
883 716 487 34 34
BglII A'GATC_T
Cuts at: 0 460 1146 2154
Size: 460 686 1008
Fragments arranged by size:
1008 686 460
Bme1580I G_kGCm'C
Cuts at: 0 1290 1999 2154
Size: 1290 709 155
Fragments arranged by size:
1290 709 155
BmgBI CAC'GTC
Cuts at: 0 1732 1819 2154
Size: 1732 87 335
Fragments arranged by size:
1732 335 87
BmrI ACTGGGnnnn_n'
Cuts at: 0 1099 2154
Size: 1099 1055
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 182 1991 2029 2154
Size: 182 1809 38 125
Fragments arranged by size:
1809 182 125 38
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 75 1884 2154
Size: 75 1809 270
Fragments arranged by size:
1809 270 75
Bpu10I CC'TnA_GC
Cuts at: 0 852 1545 2154
Size: 852 693 609
Fragments arranged by size:
852 693 609
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1201 2154
Size: 1201 953
BsaI GGTCTCn'nnnn_
Cuts at: 0 1021 2154
Size: 1021 1133
BsaAI yAC'GTr
Cuts at: 0 1656 2019 2154
Size: 1656 363 135
Fragments arranged by size:
1656 363 135
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1400 1430 1782 1812 1882 1912 2154
Size: 1400 30 352 30 70 30 242
Fragments arranged by size:
1400 352 242 70 30 30 30
BseYI C'CCAG_C
Cuts at: 0 579 2154
Size: 579 1575
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 730 1017 1090 2154
Size: 730 287 73 1064
Fragments arranged by size:
1064 730 287 73
BsiHKAI G_wGCw'C
Cuts at: 0 1803 2154
Size: 1803 351
BsmI GAATG_Cn'
Cuts at: 0 456 2154
Size: 456 1698
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1445 2154
Size: 1445 709
Bsp1286I G_dGCh'C
Cuts at: 0 1290 1803 1999 2154
Size: 1290 513 196 155
Fragments arranged by size:
1290 513 196 155
BspHI T'CATG_A
Cuts at: 0 1590 2154
Size: 1590 564
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 140 2154
Size: 140 2014
BsrDI GCAATG_nn'
Cuts at: 0 632 1893 2154
Size: 632 1261 261
Fragments arranged by size:
1261 632 261
BsrFI r'CCGG_y
Cuts at: 0 237 2154
Size: 237 1917
BsrGI T'GTAC_A
Cuts at: 0 886 2154
Size: 886 1268
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1068 1294 2154
Size: 1068 226 860
Fragments arranged by size:
1068 860 226
BstYI r'GATC_y
Cuts at: 0 38 460 684 1146 1237 2154
Size: 38 422 224 462 91 917
Fragments arranged by size:
917 462 422 224 91 38
Bsu36I CC'TnA_GG
Cuts at: 0 43 1351 1634 2154
Size: 43 1308 283 520
Fragments arranged by size:
1308 520 283 43
BtsI GCAGTG_nn'
Cuts at: 0 611 1005 1057 2154
Size: 611 394 52 1097
Fragments arranged by size:
1097 611 394 52
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1800 1835 2154
Size: 1800 35 319
Fragments arranged by size:
1800 319 35
DraI TTT'AAA
Cuts at: 0 1460 2154
Size: 1460 694
EaeI y'GGCC_r
Cuts at: 0 1389 2154
Size: 1389 765
EarI CTCTTCn'nnn_
Cuts at: 0 151 461 509 656 1019 1443 2112 2154
Size: 151 310 48 147 363 424 669 42
Fragments arranged by size:
669 424 363 310 151 147 48 42
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 75 91 205 461 485 1884 2154
Size: 75 16 114 256 24 1399 270
Fragments arranged by size:
1399 270 256 114 75 24 16
EcoNI CCTnn'n_nnAGG
Cuts at: 0 77 2154
Size: 77 2077
EcoO109I rG'GnC_Cy
Cuts at: 0 134 1629 2154
Size: 134 1495 525
Fragments arranged by size:
1495 525 134
EcoRI G'AATT_C
Cuts at: 0 955 2154
Size: 955 1199
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 796 828 1222 1254 1933 1965 2154
Size: 796 32 394 32 679 32 189
Fragments arranged by size:
796 679 394 189 32 32 32
HincII GTy'rAC
Cuts at: 0 1529 1670 1952 2109 2154
Size: 1529 141 282 157 45
Fragments arranged by size:
1529 282 157 141 45
HindIII A'AGCT_T
Cuts at: 0 353 2154
Size: 353 1801
HpaI GTT'AAC
Cuts at: 0 2109 2154
Size: 2109 45
MfeI C'AATT_G
Cuts at: 0 736 2154
Size: 736 1418
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1913 2004 2154
Size: 1913 91 150
Fragments arranged by size:
1913 150 91
MslI CAynn'nnrTG
Cuts at: 0 1012 2154
Size: 1012 1142
MspA1I CmG'CkG
Cuts at: 0 789 2154
Size: 789 1365
NdeI CA'TA_TG
Cuts at: 0 2067 2154
Size: 2067 87
NspI r_CATG'y
Cuts at: 0 2132 2154
Size: 2132 22
PflMI CCAn_nnn'nTGG
Cuts at: 0 591 1364 2154
Size: 591 773 790
Fragments arranged by size:
790 773 591
PpuMI rG'GwC_Cy
Cuts at: 0 134 1629 2154
Size: 134 1495 525
Fragments arranged by size:
1495 525 134
PshAI GACnn'nnGTC
Cuts at: 0 796 2154
Size: 796 1358
PsiI TTA'TAA
Cuts at: 0 484 2154
Size: 484 1670
PstI C_TGCA'G
Cuts at: 0 774 788 2154
Size: 774 14 1366
Fragments arranged by size:
1366 774 14
PvuII CAG'CTG
Cuts at: 0 789 2154
Size: 789 1365
SanDI GG'GwC_CC
Cuts at: 0 134 1629 2154
Size: 134 1495 525
Fragments arranged by size:
1495 525 134
SapI GCTCTTCn'nnn_
Cuts at: 0 151 461 2112 2154
Size: 151 310 1651 42
Fragments arranged by size:
1651 310 151 42
SfcI C'TryA_G
Cuts at: 0 413 770 784 1298 1580 1616 2154
Size: 413 357 14 514 282 36 538
Fragments arranged by size:
538 514 413 357 282 36 14
SmlI C'TyrA_G
Cuts at: 0 1180 1976 2154
Size: 1180 796 178
Fragments arranged by size:
1180 796 178
SnaBI TAC'GTA
Cuts at: 0 1656 2154
Size: 1656 498
StyI C'CwwG_G
Cuts at: 0 704 879 2154
Size: 704 175 1275
Fragments arranged by size:
1275 704 175
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1044 2154
Size: 1044 1110
TatI w'GTAC_w
Cuts at: 0 886 2154
Size: 886 1268
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1782 1814 2154
Size: 1782 32 340
Fragments arranged by size:
1782 340 32
XhoI C'TCGA_G
Cuts at: 0 1976 2154
Size: 1976 178
XmnI GAAnn'nnTTC
Cuts at: 0 343 2154
Size: 343 1811
Enzymes that do cut:
AcuI AlwNI ApoI AseI AvaI BanI BbsI BcgI
BglII Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaXI BseYI BsgI BsiHKAI BsmI BsmBI
Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI BstAPI BstYI
Bsu36I BtsI CspCI DraI EaeI EarI Eco57MI EcoNI
EcoO109I EcoRI Hin4I HincII HindIII HpaI MfeI MmeI
MslI MspA1I NdeI NspI PflMI PpuMI PshAI PsiI
PstI PvuII SanDI SapI SfcI SmlI SnaBI StyI
TaqII TatI TstI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI
AsiSI AvrII BaeI BamHI BanII BbeI BbvCI BciVI
BclI BfrBI BglI BlpI BmtI BsaBI BsaHI BsaWI
BseRI BsiEI BsiWI BspEI BsrBI BssHII BssSI BstBI
BstEII BstXI BstZ17I BtgI BtgZI ClaI DraIII DrdI
EagI EciI EcoICRI EcoRV FalI FseI FspI FspAI
HaeII KasI KpnI MluI MscI NaeI NarI NcoI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PfoI PmeI PmlI PpiI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SalI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SpeI SphI SrfI SspI
StuI SwaI Tth111I XbaI XcmI XmaI ZraI