(Circular) (Six-base) MAPSORT of: pFN21AA1675.seq Check: 5444 from: 1 to: 8533 pFN21AA1675 8533 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3825 6343 278 Size: 53 83 186 936 2289 2518 2468 Fragments arranged by size: 2518 2468 2289 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2220 2646 5876 1937 Size: 283 426 3230 4594 Fragments arranged by size: 4594 3230 426 283 Acc65I G'GTAC_C Cuts at: 5854 5854 Size: 8533 AclI AA'CG_TT Cuts at: 1563 6660 7033 1563 Size: 5097 373 3063 Fragments arranged by size: 5097 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2250 3044 3797 4670 4951 6596 7980 8248 Size: 618 794 753 873 281 1645 1384 268 Cuts at: 8248 1632 Size: 1917 Fragments arranged by size: 1917 1645 1384 873 794 753 618 281 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8336 AflIII A'CryG_T Cuts at: 1203 2757 2759 3795 4857 7432 8409 1203 Size: 1554 2 1036 1062 2575 977 1327 Fragments arranged by size: 2575 1554 1327 1062 1036 977 2 AgeI A'CCGG_T Cuts at: 7364 7364 Size: 8533 AhdI GACnn_n'nnGTC Cuts at: 5431 7262 5431 Size: 1831 6702 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8533 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4784 1184 Size: 3600 4933 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2675 2707 2675 Size: 32 8501 AlwNI CAG_nnn'CTG Cuts at: 2285 4051 4192 4411 4869 6276 7848 2285 Size: 1766 141 219 458 1407 1572 2970 Fragments arranged by size: 2970 1766 1572 1407 458 219 141 ApaI G_GGCC'C Cuts at: 2339 3100 3729 3979 4379 4438 2339 Size: 761 629 250 400 59 6434 Fragments arranged by size: 6434 761 629 400 250 59 ApaLI G'TGCA_C Cuts at: 2627 6590 7746 2627 Size: 3963 1156 3414 Fragments arranged by size: 3963 3414 1156 ApoI r'AATT_y Cuts at: 1493 5842 6068 6220 8329 1493 Size: 4349 226 152 2109 1697 Fragments arranged by size: 4349 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 7087 160 Size: 872 6055 1606 Fragments arranged by size: 6055 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8533 AvaI C'yCGr_G Cuts at: 1870 1943 2355 2813 3093 3379 3487 3547 3728 Size: 73 412 458 280 286 108 60 181 Cuts at: 3728 3970 4278 4756 5126 5444 5858 1870 Size: 242 308 478 370 318 414 4545 Fragments arranged by size: 4545 478 458 414 412 370 318 308 286 280 242 181 108 73 60 BamHI G'GATC_C Cuts at: 5863 6230 5863 Size: 367 8166 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2350 2742 3511 3964 3997 Size: 325 217 627 563 392 769 453 33 Cuts at: 3997 4064 4505 5079 5854 7309 618 Size: 67 441 574 775 1455 1842 Fragments arranged by size: 1842 1455 775 769 627 574 563 453 441 392 325 217 67 33 BanII G_rGCy'C Cuts at: 1391 2076 2201 2262 2339 2688 2808 3100 3617 Size: 685 125 61 77 349 120 292 517 Cuts at: 3617 3729 3872 3979 4157 4237 4379 4438 4470 Size: 112 143 107 178 80 142 59 32 Cuts at: 4470 4648 5451 5852 1391 Size: 178 803 401 4072 Fragments arranged by size: 4072 803 685 517 401 349 292 178 178 143 142 125 120 112 107 80 77 61 59 32 BbeI G_GCGC'C Cuts at: 2746 3515 3968 4068 4509 2746 Size: 769 453 100 441 6770 Fragments arranged by size: 6770 769 453 441 100 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2238 2457 3128 3785 3997 5217 5736 Size: 971 339 219 671 657 212 1220 519 Cuts at: 5736 928 Size: 3725 Fragments arranged by size: 3725 1220 971 671 657 519 339 219 212 BbvCI CC'TCA_GC Cuts at: 2799 5532 5610 2799 Size: 2733 78 5722 Fragments arranged by size: 5722 2733 78 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2108 2142 2995 3029 4842 Size: 34 50 34 87 34 853 34 1813 Cuts at: 4842 4876 6724 6758 1903 Size: 34 1848 34 3678 Fragments arranged by size: 3678 1848 1813 853 87 50 34 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6425 7635 6425 Size: 1210 7323 BclI T'GATC_A Cuts at: 1548 1548 Size: 8533 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4452 4748 5498 Size: 107 122 71 631 605 2780 296 750 Cuts at: 5498 7144 136 Size: 1646 1525 Fragments arranged by size: 2780 1646 1525 750 631 605 296 122 107 71 BglII A'GATC_T Cuts at: 2442 4878 8528 2442 Size: 2436 3650 2447 Fragments arranged by size: 3650 2447 2436 BlpI GC'TnA_GC Cuts at: 5950 5950 Size: 8533 Bme1580I G_kGCm'C Cuts at: 1790 2339 2355 2631 2660 3100 3710 3729 3979 Size: 549 16 276 29 440 610 19 250 Cuts at: 3979 4319 4379 4438 4535 5320 6594 7750 1790 Size: 340 60 59 97 785 1274 1156 2573 Fragments arranged by size: 2573 1274 1156 785 610 549 440 340 276 250 97 60 59 29 19 16 BmgBI CAC'GTC Cuts at: 1318 2407 3135 3889 4348 4483 4996 5356 5796 Size: 1089 728 754 459 135 513 360 440 Cuts at: 5796 1318 Size: 4055 Fragments arranged by size: 4055 1089 754 728 513 459 440 360 135 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3642 4299 4935 Size: 416 53 83 399 24 2225 657 636 Cuts at: 4935 5314 6264 7222 442 Size: 379 950 958 1753 Fragments arranged by size: 2225 1753 958 950 657 636 416 399 379 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2036 1056 Size: 980 7553 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2912 3460 3773 4748 4970 5402 5808 5826 Size: 1673 548 313 975 222 432 406 18 Cuts at: 5826 7193 1239 Size: 1367 2579 Fragments arranged by size: 2579 1673 1367 975 548 432 406 313 222 18 Bpu10I CC'TnA_GC Cuts at: 2799 4418 5532 5610 2799 Size: 1619 1114 78 5722 Fragments arranged by size: 5722 1619 1114 78 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2495 2792 3131 3593 4274 5069 6658 7523 7821 Size: 297 339 462 681 795 1589 865 298 Cuts at: 7821 2495 Size: 3207 Fragments arranged by size: 3207 1589 865 795 681 462 339 298 297 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3685 4703 7196 882 Size: 617 2186 1018 2493 2219 Fragments arranged by size: 2493 2219 2186 1018 617 BsaAI yAC'GTr Cuts at: 493 1204 2632 2760 3085 4860 6324 493 Size: 711 1428 128 325 1775 1464 2702 Fragments arranged by size: 2702 1775 1464 1428 711 325 128 BsaBI GATnn'nnATC Cuts at: 2484 8527 2484 Size: 6043 2490 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2643 2743 3512 3636 Size: 53 83 186 936 1110 100 769 124 Cuts at: 3636 3822 3965 4065 4284 4506 6340 6722 7357 Size: 186 143 100 219 222 1834 382 635 Cuts at: 7357 275 Size: 1451 Fragments arranged by size: 1834 1451 1110 936 769 635 382 222 219 186 186 143 124 100 100 83 53 BsaWI w'CCGG_w Cuts at: 4800 4998 6966 7364 7638 7785 8196 4800 Size: 198 1968 398 274 147 411 5137 Fragments arranged by size: 5137 1968 411 398 274 198 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2675 2705 2912 2942 3458 Size: 30 165 30 1272 30 207 30 516 Cuts at: 3458 3488 3499 3529 4922 4952 5735 5765 1178 Size: 30 11 30 1393 30 783 30 3946 Fragments arranged by size: 3946 1393 1272 783 516 207 165 30 30 30 30 30 30 30 30 11 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2630 2696 2816 2933 3301 3746 4415 5375 Size: 1444 66 120 117 368 445 669 960 Cuts at: 5375 5378 5399 5486 5495 5727 1186 Size: 3 21 87 9 232 3992 Fragments arranged by size: 3992 1444 960 669 445 368 232 120 117 87 66 21 9 3 BseYI C'CCAG_C Cuts at: 2245 2332 3050 3398 3431 3664 4014 4150 4501 Size: 87 718 348 33 233 350 136 351 Cuts at: 4501 4548 7736 8232 2245 Size: 47 3188 496 2546 Fragments arranged by size: 3188 2546 718 496 351 350 348 233 136 87 47 33 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4882 5701 1163 Size: 573 3146 819 3995 Fragments arranged by size: 3995 3146 819 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3581 4670 4960 5983 6744 7772 Size: 275 186 1580 1089 290 1023 761 1028 Cuts at: 7772 1540 Size: 2301 Fragments arranged by size: 2301 1580 1089 1028 1023 761 290 275 186 BsiHKAI G_wGCw'C Cuts at: 2262 2534 2631 2808 5100 6594 6679 7750 2262 Size: 272 97 177 2292 1494 85 1071 3045 Fragments arranged by size: 3045 2292 1494 1071 272 177 97 85 BsiWI C'GTAC_G Cuts at: 2205 2205 Size: 8533 BsmI GAATG_Cn' Cuts at: 6044 6137 6044 Size: 93 8440 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2076 2201 2262 2339 2355 2534 2631 Size: 399 286 125 61 77 16 179 97 Cuts at: 2631 2660 2688 2808 3100 3617 3710 3729 3872 Size: 29 28 120 292 517 93 19 143 Cuts at: 3872 3979 4157 4237 4319 4379 4438 4470 4535 Size: 107 178 80 82 60 59 32 65 Cuts at: 4535 4648 5100 5320 5451 5852 6594 6679 7750 Size: 113 452 220 131 401 742 85 1071 Cuts at: 7750 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 517 452 401 399 292 286 220 179 178 143 131 125 120 113 107 97 93 85 82 80 77 65 61 60 59 32 29 28 19 16 BspEI T'CCGG_A Cuts at: 4998 8196 4998 Size: 3198 5335 BspHI T'CATG_A Cuts at: 6422 8152 6422 Size: 1730 6803 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2094 2745 4671 4854 5888 844 Size: 1250 651 1926 183 1034 3489 Fragments arranged by size: 3489 1926 1250 1034 651 183 BsrBI CCG'CTC Cuts at: 1394 1823 2866 3075 4561 4962 6420 1394 Size: 429 1043 209 1486 401 1458 3507 Fragments arranged by size: 3507 1486 1458 1043 429 401 209 BsrDI GCAATG_nn' Cuts at: 65 1248 4098 5715 7028 7202 65 Size: 1183 2850 1617 1313 174 1396 Fragments arranged by size: 2850 1617 1396 1313 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3234 3327 4508 5076 7177 7364 1700 Size: 110 1424 93 1181 568 2101 187 2869 Fragments arranged by size: 2869 2101 1424 1181 568 187 110 93 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8533 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8533 BssSI C'ACGA_G Cuts at: 2846 5093 5099 6593 7605 2846 Size: 2247 6 1494 1012 3774 Fragments arranged by size: 3774 2247 1494 1012 6 BstAPI GCAn_nnn'nTGC Cuts at: 794 2859 4100 5049 794 Size: 2065 1241 949 4278 Fragments arranged by size: 4278 2065 1241 949 BstBI TT'CG_AA Cuts at: 3015 4979 7337 3015 Size: 1964 2358 4211 Fragments arranged by size: 4211 2358 1964 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8533 BstXI CCAn_nnnn'nTGG Cuts at: 2146 3655 4487 2146 Size: 1509 832 6192 Fragments arranged by size: 6192 1509 832 BstYI r'GATC_y Cuts at: 1972 2442 4878 5863 6230 6615 6632 8084 8170 Size: 470 2436 985 367 385 17 1452 86 Cuts at: 8170 8182 8528 1972 Size: 12 346 1977 Fragments arranged by size: 2436 1977 1452 985 470 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4835 4913 4835 Size: 78 8455 BtgI C'CryG_G Cuts at: 513 1065 2145 3507 3834 3858 4638 5502 513 Size: 552 1080 1362 327 24 780 864 3544 Fragments arranged by size: 3544 1362 1080 864 780 552 327 24 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2553 4075 487 Size: 683 56 1327 1522 4945 Fragments arranged by size: 4945 1522 1327 683 56 BtsI GCAGTG_nn' Cuts at: 2467 3814 5320 6049 6843 6863 2467 Size: 1347 1506 729 794 20 4137 Fragments arranged by size: 4137 1506 1347 794 729 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8533 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3268 3303 529 Size: 35 2704 35 5759 Fragments arranged by size: 5759 2704 35 35 DraI TTT'AAA Cuts at: 5667 5837 5667 Size: 170 8363 DraIII CAC_nnn'GTG Cuts at: 2559 2559 Size: 8533 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5158 7540 809 Size: 507 3842 2382 1802 Fragments arranged by size: 3842 2382 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2091 2836 3289 Size: 54 1425 50 275 18 261 745 453 Cuts at: 3289 3578 3990 4667 5406 5499 5980 6869 8498 Size: 289 412 677 739 93 481 889 1629 Cuts at: 8498 8 Size: 43 Fragments arranged by size: 1629 1425 889 745 739 677 481 453 412 289 275 261 93 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3578 4667 5980 1537 Size: 275 1766 1089 1313 4090 Fragments arranged by size: 4090 1766 1313 1089 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2723 4688 4981 6463 1353 Size: 1370 1965 293 1482 3423 Fragments arranged by size: 3423 1965 1482 1370 293 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2969 3673 3919 4766 5306 7120 Size: 1064 138 1380 704 246 847 540 1814 Cuts at: 7120 7494 7640 387 Size: 374 146 1280 Fragments arranged by size: 1814 1380 1280 1064 847 704 540 374 246 146 138 EcoICRI GAG'CTC Cuts at: 2260 2806 2260 Size: 546 7987 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2250 2912 3044 3460 3773 3797 4670 Size: 393 618 662 132 416 313 24 873 Cuts at: 4670 4748 4951 4970 5402 5808 5826 6596 7193 Size: 78 203 19 432 406 18 770 597 Cuts at: 7193 7980 8248 1239 Size: 787 268 1524 Fragments arranged by size: 1524 873 787 770 662 618 597 432 416 406 393 313 268 203 132 78 24 19 18 EcoNI CCTnn'n_nnAGG Cuts at: 2678 2798 4838 5384 2678 Size: 120 2040 546 5827 Fragments arranged by size: 5827 2040 546 120 EcoO109I rG'GnC_Cy Cuts at: 2083 2212 2336 2791 2818 3725 3975 3976 4375 Size: 129 124 455 27 907 250 1 399 Cuts at: 4375 4376 5131 2083 Size: 1 755 5485 Fragments arranged by size: 5485 907 755 455 399 250 129 124 27 1 1 EcoRI G'AATT_C Cuts at: 5842 6220 5842 Size: 378 8155 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 5654 5654 Size: 8533 FspI TGC'GCA Cuts at: 7039 7039 Size: 8533 HaeII r_GCGC'y Cuts at: 1678 2746 3093 3334 3515 3968 4068 4509 7680 Size: 1068 347 241 181 453 100 441 3171 Cuts at: 7680 1678 Size: 2531 Fragments arranged by size: 3171 2531 1068 453 441 347 241 181 100 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4256 4288 5417 5449 7174 Size: 32 135 32 2684 32 1129 32 1725 Cuts at: 7174 7206 7248 7280 1373 Size: 32 42 32 2626 Fragments arranged by size: 2684 2626 1725 1129 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3826 5877 6123 669 Size: 988 281 1888 2051 246 3079 Fragments arranged by size: 3079 2051 1888 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8533 HpaI GTT'AAC Cuts at: 6123 6123 Size: 8533 KasI G'GCGC_C Cuts at: 2742 3511 3964 4064 4505 2742 Size: 769 453 100 441 6770 Fragments arranged by size: 6770 769 453 441 100 KpnI G_GTAC'C Cuts at: 5858 5858 Size: 8533 MfeI C'AATT_G Cuts at: 6132 6132 Size: 8533 MluI A'CGCG_T Cuts at: 8409 8409 Size: 8533 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2994 3249 3780 4848 5160 5778 7647 7831 Size: 1689 255 531 1068 312 618 1869 184 Cuts at: 7831 1305 Size: 2007 Fragments arranged by size: 2007 1869 1689 1068 618 531 312 255 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2093 3291 10 Size: 54 1425 343 261 1198 5252 Fragments arranged by size: 5252 1425 1198 343 261 54 MslI CAynn'nnrTG Cuts at: 518 1166 2144 2522 4133 4485 6491 6850 7009 Size: 648 978 378 1611 352 2006 359 159 Cuts at: 7009 518 Size: 2042 Fragments arranged by size: 2042 2006 1611 978 648 378 359 352 159 MspA1I CmG'CkG Cuts at: 1606 2505 2697 2835 3003 3064 3604 3719 4048 Size: 899 192 138 168 61 540 115 329 Cuts at: 4048 4411 4678 4990 5049 5609 5949 6626 7774 Size: 363 267 312 59 560 340 677 1148 Cuts at: 7774 8019 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 899 677 560 540 363 340 329 312 267 245 192 168 138 115 61 59 NaeI GCC'GGC Cuts at: 3236 4510 3236 Size: 1274 7259 NarI GG'CG_CC Cuts at: 2743 3512 3965 4065 4506 2743 Size: 769 453 100 441 6770 Fragments arranged by size: 6770 769 453 441 100 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 7981 NdeI CA'TA_TG Cuts at: 387 3188 5704 387 Size: 2801 2516 3216 Fragments arranged by size: 3216 2801 2516 NgoMIV G'CCGG_C Cuts at: 3234 4508 3234 Size: 1274 7259 NheI G'CTAG_C Cuts at: 1052 2032 1052 Size: 980 7553 NotI GC'GGCC_GC Cuts at: 5980 5980 Size: 8533 NspI r_CATG'y Cuts at: 1132 1229 3799 5299 5891 7436 1132 Size: 97 2570 1500 592 1545 2229 Fragments arranged by size: 2570 2229 1545 1500 592 97 PasI CC'CwG_GG Cuts at: 1348 2279 2653 3655 5755 1348 Size: 931 374 1002 2100 4126 Fragments arranged by size: 4126 2100 1002 931 374 PciI A'CATG_T Cuts at: 3795 7432 3795 Size: 3637 4896 PflMI CCAn_nnn'nTGG Cuts at: 1108 2285 1108 Size: 1177 7356 PmeI GTTT'AAAC Cuts at: 5837 5837 Size: 8533 PmlI CAC'GTG Cuts at: 2632 2760 3085 4860 2632 Size: 128 325 1775 6305 Fragments arranged by size: 6305 1775 325 128 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2675 2707 2855 2887 3722 3754 6602 6634 8115 Size: 32 148 32 835 32 2848 32 1481 Cuts at: 8115 8147 2675 Size: 32 3061 Fragments arranged by size: 3061 2848 1481 835 148 32 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2083 5131 2083 Size: 3048 5485 PshAI GACnn'nnGTC Cuts at: 1613 2943 1613 Size: 1330 7203 PsiI TTA'TAA Cuts at: 6103 6103 Size: 8533 PspOMI G'GGCC_C Cuts at: 2335 3096 3725 3975 4375 4434 2335 Size: 761 629 250 400 59 6434 Fragments arranged by size: 6434 761 629 400 250 59 PstI C_TGCA'G Cuts at: 830 4773 5885 830 Size: 3943 1112 3478 Fragments arranged by size: 3943 3478 1112 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8533 PvuII CAG'CTG Cuts at: 2505 3719 4048 5609 2505 Size: 1214 329 1561 5429 Fragments arranged by size: 5429 1561 1214 329 RsrII CG'GwC_CG Cuts at: 4228 4228 Size: 8533 SacI G_AGCT'C Cuts at: 2262 2808 2262 Size: 546 7987 SalI G'TCGA_C Cuts at: 1936 5875 1936 Size: 3939 4594 SapI GCTCTTCn'nnn_ Cuts at: 4688 4688 Size: 8533 SbfI CC_TGCA'GG Cuts at: 5885 5885 Size: 8533 ScaI AGT'ACT Cuts at: 3467 4112 4952 6781 8493 3467 Size: 645 840 1829 1712 3507 Fragments arranged by size: 3507 1829 1712 840 645 SfcI C'TryA_G Cuts at: 826 1045 4603 4769 5881 7016 7697 7888 826 Size: 219 3558 166 1112 1135 681 191 1471 Fragments arranged by size: 3558 1471 1135 1112 681 219 191 166 SfoI GGC'GCC Cuts at: 2744 3513 3966 4066 4507 2744 Size: 769 453 100 441 6770 Fragments arranged by size: 6770 769 453 441 100 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8533 SgrAI Cr'CCGG_yG Cuts at: 1700 3327 4508 1700 Size: 1627 1181 5725 Fragments arranged by size: 5725 1627 1181 SmaI CCC'GGG Cuts at: 1872 2357 3381 3489 3549 3972 4280 5128 5860 Size: 485 1024 108 60 423 308 848 732 Cuts at: 5860 1872 Size: 4545 Fragments arranged by size: 4545 1024 848 732 485 423 308 108 60 SmlI C'TyrA_G Cuts at: 820 1017 1943 2510 2807 3146 3608 4289 5084 Size: 197 926 567 297 339 462 681 795 Cuts at: 5084 6637 7538 7800 820 Size: 1553 901 262 1553 Fragments arranged by size: 1553 1553 926 901 795 681 567 462 339 297 262 197 SnaBI TAC'GTA Cuts at: 493 6324 493 Size: 5831 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8533 SphI G_CATG'C Cuts at: 1132 5891 1132 Size: 4759 3774 SspI AAT'ATT Cuts at: 5 52 6457 5 Size: 47 6405 2081 Fragments arranged by size: 6405 2081 47 StuI AGG'CCT Cuts at: 1517 2954 5331 1517 Size: 1437 2377 4719 Fragments arranged by size: 4719 2377 1437 StyI C'CwwG_G Cuts at: 513 1065 2013 2190 2821 2949 4371 5972 513 Size: 552 948 177 631 128 1422 1601 3074 Fragments arranged by size: 3074 1601 1422 948 631 552 177 128 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3644 3857 4245 6560 6577 6730 6915 Size: 1255 2064 213 388 2315 17 153 185 Cuts at: 6915 7360 325 Size: 445 1498 Fragments arranged by size: 2315 2064 1498 1255 445 388 213 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2514 2754 2883 Size: 274 80 33 51 1443 537 240 129 Cuts at: 2883 2928 3465 4110 4950 6779 8491 96 Size: 45 537 645 840 1829 1712 138 Fragments arranged by size: 1829 1712 1443 840 645 537 537 274 240 138 129 80 51 45 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2975 3007 3499 3531 5735 5767 8493 8525 2975 Size: 32 492 32 2204 32 2726 32 2983 Fragments arranged by size: 2983 2726 2204 492 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8533 XbaI T'CTAG_A Cuts at: 5869 5869 Size: 8533 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2311 3406 3947 2311 Size: 1095 541 6897 Fragments arranged by size: 6897 1095 541 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8533 XmaI C'CCGG_G Cuts at: 1870 2355 3379 3487 3547 3970 4278 5126 5858 Size: 485 1024 108 60 423 308 848 732 Cuts at: 5858 1870 Size: 4545 Fragments arranged by size: 4545 1024 848 732 485 423 308 108 60 XmnI GAAnn'nnTTC Cuts at: 2235 3038 3125 3182 4880 5024 6662 2235 Size: 803 87 57 1698 144 1638 4106 Fragments arranged by size: 4106 1698 1638 803 144 87 57 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3823 6341 276 Size: 53 83 186 936 2289 2518 2468 Fragments arranged by size: 2518 2468 2289 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BfrBI BplI BsmBI BstZ17I EcoRV FseI FspAI NruI NsiI PacI PfoI PspXI PsrI SacII SanDI SexAI SfiI SrfI SwaI