Restriction map for
pFN21AA1558
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# NO CUT ENZYMES ( 103 enzymes) #
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Acc65I AccIII AclI AflII AgeI
AhdI Aor51HI AscI Asp718I AspEI
AviII AvrII BbrPI BcgI BclI
BfrI BlnI BsaAI BsaBI BsaWI
BseAI BsiEI BsiWI Bsp1407I BspDI
BspEI BsrBI BsrFI BsrGI BssHII
BssSI BstBI BstEII BstPI Cfr10I
ClaI CpoI DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRI FauI FbaI FseI
FspI HincII HindII HindIII HpaI
KpnI Ksp632I KspI MamI MfeI
MluI MroI MspA1I MunI NaeI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI Psp1406I PvuI
PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SwaI Van91I XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AatII 1662 GACGTC GACGT^C
AccII 2550 CGCG CG^CG
Alw44I 1405 GTGCAC G^TGCAC
AocI 1508 CCTTAGG CC^T[ACGT]AGG
ApaI 1422 GGGCCC GGGCC^C
ApaLI 1405 GTGCAC G^TGCAC
AseI 1296 ATTAAT AT^TAAT
AsnI 1296 ATTAAT AT^TAAT
AspI 1468 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1008 GGATCC G^GATCC
BanI 2553 GGCGCC G^G[CT][GA]CC
BbeI 2553 GGCGCC GGCGC^C
BbvCI 1345 GCTGAGG CCTCAGC(-5/-2)
BciVI 228 GTATCC GTATCC(6/5)
BcnI 1028 CCCGG CC^[GC]GG
BglI 869 GCCCACCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 2403 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2403 GCTCAGC GC^T[ACGT]AGC
BsaI 668 GAGACC GGTCTC(1/5)
BsmI 1885 GCATTC GAATGC(1/-1)
BspHI 948 TCATGA T^CATGA
BspLU11I 1130 ACATGT A^CATGT
BspMI 1552 ACCTGC ACCTGC(4/8)
BsrDI 1727 GCAATG GCAATG(2/0)
Bst1107I 1143 GTATAC GTA^TAC
BstAPI 537 GCAAGATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 2550 CGCG CG^CG
BstXI 2038 CCACCCAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1143 GTATAC GTA^TAC
Bsu36I 1508 CCTTAGG CC^T[ACGT]AGG
CelII 2403 GCTCAGC GC^T[ACGT]AGC
DraI 1591 TTTAAA TTT^AAA
Eco81I 1508 CCTTAGG CC^T[ACGT]AGG
EcoRV 1050 GATATC GAT^ATC
EcoT22I 629 ATGCAT ATGCA^T
HaeII 2553 GGCGCC [GA]GCGC^[CT]
HapII 1029 CCGG C^CGG
HgaI 2512 GACGC GACGC(5/10)
HpaII 1029 CCGG C^CGG
KasI 2553 GGCGCC G^GCGCC
MspI 1029 CCGG C^CGG
MvnI 2550 CGCG CG^CG
NarI 2553 GGCGCC GG^CGCC
NciI 1028 CCCGG CC^[GC]GG
NsiI 629 ATGCAT ATGCA^T
PflFI 1468 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1091 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 2433 GACTGCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1296 ATTAAT AT^TAAT
PstI 2534 CTGCAG CTGCA^G
RcaI 948 TCATGA T^CATGA
SexAI 2607 ACCAGGT A^CC[AT]GGT
SfoI 2553 GGCGCC GGC^GCC
StuI 2560 AGGCCT AGG^CCT
Tsp45I 2612 GTGAC ^GT[GC]AC
Tth111I 1468 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 20 enzymes) #
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AcyI 1662 GACGTC G[GA]^CG[CT]C
2553 GGCGCC
AflIII 511 ACGTGT A^C[GA][CT]GT
1130 ACATGT
AlwNI 1946 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
2429 CAGAGACTG
BalI 2312 TGGCCA TGG^CCA
2592 TGGCCA
BanII 700 GAGCCC G[GA]GC[CT]^C
1422 GGGCCC
BsaHI 1662 GACGTC G[GA]^CG[CT]C
2553 GGCGCC
BsmBI 1489 GAGACG CGTCTC(1/5)
2149 GAGACG
DraIII 641 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
2606 CACCAGGTG
EaeI 2312 TGGCCA [CT]^GGCC[GA]
2592 TGGCCA
EcoT14I 2249 CCATGG C^C[AT][AT]GG
2315 CCATGG
Hin1I 1662 GACGTC G[GA]^CG[CT]C
2553 GGCGCC
HphI 1255 GGTGA GGTGA(8/7)
2611 GGTGA
MluNI 2312 TGGCCA TGG^CCA
2592 TGGCCA
MscI 2312 TGGCCA TGG^CCA
2592 TGGCCA
MslI 622 CATTCAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2616 CACTTCAGTG
NcoI 2249 CCATGG C^CATGG
2315 CCATGG
NspI 1130 ACATGT [GA]CATG^[CT]
1807 GCATGT
SfcI 2414 CTGTAG C^T[GA][CT]AG
2534 CTGCAG
SspI 760 AATATT AAT^ATT
1789 AATATT
StyI 2249 CCATGG C^C[AT][AT]GG
2315 CCATGG
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# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI Asp700I AspHI AvaI
AvaII BbsI BbvI BfaI BglII
BmyI BpmI BpuAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EcoO109I EcoRII Fnu4HI FokI HaeIII
HhaI HinP1I HinfI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MvaI MwoI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SmlI TaqI
TfiI Tru9I TseI Tsp509I TspRI
TthHB8I XbaI XhoII XmnI