(Circular) (Six-base) MAPSORT of: pFN21AA1558.seq Check: 3575 from: 1 to: 7342 pFN21AA1558 7342 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3671 5152 278 Size: 53 83 186 936 2135 1481 2468 Fragments arranged by size: 2468 2135 1481 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2454 3149 4490 4685 1937 Size: 517 695 1341 195 4594 Fragments arranged by size: 4594 1341 695 517 195 Acc65I G'GTAC_C Cuts at: 4663 4663 Size: 7342 AclI AA'CG_TT Cuts at: 1563 5469 5842 1563 Size: 3906 373 3063 Fragments arranged by size: 3906 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2096 2359 3978 4608 5405 6789 7057 1632 Size: 464 263 1619 630 797 1384 268 1917 Fragments arranged by size: 1917 1619 1384 797 630 464 268 263 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7145 AflIII A'CryG_T Cuts at: 1203 2516 3135 6241 7218 1203 Size: 1313 619 3106 977 1327 Fragments arranged by size: 3106 1327 1313 977 619 AgeI A'CCGG_T Cuts at: 6173 6173 Size: 7342 AhdI GACnn_n'nnGTC Cuts at: 6071 6071 Size: 7342 AleI CACnn'nnGTG Cuts at: 1166 4625 1166 Size: 3459 3883 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7342 AlwNI CAG_nnn'CTG Cuts at: 3956 4439 5085 6657 3956 Size: 483 646 1572 4641 Fragments arranged by size: 4641 1572 646 483 ApaI G_GGCC'C Cuts at: 3431 3431 Size: 7342 ApaLI G'TGCA_C Cuts at: 3410 5399 6555 3410 Size: 1989 1156 4197 Fragments arranged by size: 4197 1989 1156 ApoI r'AATT_y Cuts at: 1493 2015 2688 3183 4651 4877 5029 7138 1493 Size: 522 673 495 1468 226 152 2109 1697 Fragments arranged by size: 2109 1697 1468 673 522 495 226 152 AseI AT'TA_AT Cuts at: 160 1032 3302 5896 160 Size: 872 2270 2594 1606 Fragments arranged by size: 2594 2270 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7342 AvaI C'yCGr_G Cuts at: 1870 1943 3922 4532 4583 4667 1870 Size: 73 1979 610 51 84 4545 Fragments arranged by size: 4545 1979 610 84 73 51 BamHI G'GATC_C Cuts at: 3013 4672 5039 3013 Size: 1659 367 5316 Fragments arranged by size: 5316 1659 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4558 4663 6118 618 Size: 325 217 627 2771 105 1455 1842 Fragments arranged by size: 2771 1842 1455 627 325 217 105 BanII G_rGCy'C Cuts at: 1391 2709 3431 4661 1391 Size: 1318 722 1230 4072 Fragments arranged by size: 4072 1318 1230 722 BbeI G_GCGC'C Cuts at: 4562 4562 Size: 7342 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2944 2999 3367 4179 4438 928 Size: 971 1045 55 368 812 259 3832 Fragments arranged by size: 3832 1045 971 812 368 259 55 BbvCI CC'TCA_GC Cuts at: 3351 3351 Size: 7342 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5533 5567 1903 Size: 34 3596 34 3678 Fragments arranged by size: 3678 3596 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2244 5234 6444 2244 Size: 2990 1210 3142 Fragments arranged by size: 3142 2990 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7342 BfrBI ATG'CAT Cuts at: 2636 2636 Size: 7342 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2880 5953 136 Size: 107 122 71 631 605 1208 3073 1525 Fragments arranged by size: 3073 1525 1208 631 605 122 107 71 BglII A'GATC_T Cuts at: 2868 3463 3544 7337 2868 Size: 595 81 3793 2873 Fragments arranged by size: 3793 2873 595 81 BlpI GC'TnA_GC Cuts at: 4409 4759 4409 Size: 350 6992 Bme1580I G_kGCm'C Cuts at: 1790 3414 3431 5403 6559 1790 Size: 1624 17 1972 1156 2573 Fragments arranged by size: 2573 1972 1624 1156 17 BmgBI CAC'GTC Cuts at: 1318 2517 4185 1318 Size: 1199 1668 4475 Fragments arranged by size: 4475 1668 1199 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2300 4266 5073 Size: 416 53 83 399 24 883 1966 807 Cuts at: 5073 6031 442 Size: 958 1753 Fragments arranged by size: 1966 1753 958 883 807 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7342 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4239 4239 Size: 7342 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2987 4144 4209 6002 1239 Size: 1748 1157 65 1793 2579 Fragments arranged by size: 2579 1793 1748 1157 65 Bpu10I CC'TnA_GC Cuts at: 2504 2666 3351 4193 2504 Size: 162 685 842 5653 Fragments arranged by size: 5653 842 685 162 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2187 2852 4482 5467 6332 6630 2187 Size: 665 1630 985 865 298 2899 Fragments arranged by size: 2899 1630 985 865 665 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2667 6005 882 Size: 617 1168 3338 2219 Fragments arranged by size: 3338 2219 1168 617 BsaAI yAC'GTr Cuts at: 493 1204 5133 493 Size: 711 3929 2702 Fragments arranged by size: 3929 2702 711 BsaBI GATnn'nnATC Cuts at: 7336 7336 Size: 7342 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3668 4559 5149 5531 Size: 53 83 186 936 2135 891 590 382 Cuts at: 5531 6166 275 Size: 635 1451 Fragments arranged by size: 2135 1451 936 891 635 590 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5775 6173 6447 6594 7005 5775 Size: 398 274 147 411 6112 Fragments arranged by size: 6112 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7117 Fragments arranged by size: 7117 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2582 4148 4358 4538 1186 Size: 1396 1566 210 180 3990 Fragments arranged by size: 3990 1566 1396 210 180 BseYI C'CCAG_C Cuts at: 3995 4161 4571 6545 7041 3995 Size: 166 410 1974 496 4296 Fragments arranged by size: 4296 1974 496 410 166 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3467 3487 4313 1163 Size: 573 1731 20 826 4192 Fragments arranged by size: 4192 1731 826 573 20 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4792 5553 6581 1540 Size: 275 186 2791 761 1028 2301 Fragments arranged by size: 2791 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3414 4096 4130 4199 5403 5488 6559 3414 Size: 682 34 69 1204 85 1071 4197 Fragments arranged by size: 4197 1204 1071 682 85 69 34 BsmI GAATG_Cn' Cuts at: 3890 4853 4946 3890 Size: 963 93 6286 Fragments arranged by size: 6286 963 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3488 4148 3488 Size: 660 6682 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2709 3414 3431 4096 4130 4199 4661 Size: 399 919 705 17 665 34 69 462 Cuts at: 4661 5403 5488 6559 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 919 742 705 665 462 399 85 69 34 17 BspEI T'CCGG_A Cuts at: 7005 7005 Size: 7342 BspHI T'CATG_A Cuts at: 2953 5231 6961 2953 Size: 2278 1730 3334 Fragments arranged by size: 3334 2278 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3566 4697 844 Size: 2722 1131 3489 Fragments arranged by size: 3489 2722 1131 BsrBI CCG'CTC Cuts at: 1394 1823 5229 1394 Size: 429 3406 3507 Fragments arranged by size: 3507 3406 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3739 5837 6011 65 Size: 1183 2491 2098 174 1396 Fragments arranged by size: 2491 2098 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5986 6173 1700 Size: 110 4176 187 2869 Fragments arranged by size: 4176 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7342 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7342 BssSI C'ACGA_G Cuts at: 5402 6414 5402 Size: 1012 6330 BstAPI GCAn_nnn'nTGC Cuts at: 794 2548 794 Size: 1754 5588 BstBI TT'CG_AA Cuts at: 6146 6146 Size: 7342 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7342 BstXI CCAn_nnnn'nTGG Cuts at: 4050 4050 Size: 7342 BstYI r'GATC_y Cuts at: 1972 2868 2945 3013 3463 3544 4112 4672 5039 Size: 896 77 68 450 81 568 560 367 Cuts at: 5039 5424 5441 6893 6979 6991 7337 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 896 568 560 450 385 367 346 86 81 77 68 17 12 BstZ17I GTA'TAC Cuts at: 3150 3150 Size: 7342 Bsu36I CC'TnA_GG Cuts at: 3514 3514 Size: 7342 BtgI C'CryG_G Cuts at: 513 1065 4087 4254 4320 513 Size: 552 3022 167 66 3535 Fragments arranged by size: 3535 3022 552 167 66 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2592 3871 487 Size: 683 56 1366 1279 3958 Fragments arranged by size: 3958 1366 1279 683 56 BtsI GCAGTG_nn' Cuts at: 4006 4296 4318 4858 5652 5672 4006 Size: 290 22 540 794 20 5676 Fragments arranged by size: 5676 794 540 290 22 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7342 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7307 DraI TTT'AAA Cuts at: 3598 4646 3598 Size: 1048 6294 DraIII CAC_nnn'GTG Cuts at: 2651 4616 2651 Size: 1965 5377 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6349 809 Size: 507 5033 1802 Fragments arranged by size: 5033 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4317 4597 4789 Size: 54 1425 50 275 18 2487 280 192 Cuts at: 4789 5678 7307 8 Size: 889 1629 43 Fragments arranged by size: 2487 1629 1425 889 280 275 192 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4789 1537 Size: 275 2977 4090 Fragments arranged by size: 4090 2977 275 EarI CTCTTCn'nnn_ Cuts at: 1353 5272 1353 Size: 3919 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5929 6303 6449 387 Size: 1064 138 4340 374 146 1280 Fragments arranged by size: 4340 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2096 2359 2987 3978 4144 4209 4608 Size: 393 464 263 628 991 166 65 399 Cuts at: 4608 5405 6002 6789 7057 1239 Size: 797 597 787 268 1524 Fragments arranged by size: 1524 991 797 787 628 597 464 399 393 268 263 166 65 EcoO109I rG'GnC_Cy Cuts at: 3097 3427 3428 3097 Size: 330 1 7011 Fragments arranged by size: 7011 330 1 EcoRI G'AATT_C Cuts at: 4651 5029 4651 Size: 378 6964 EcoRV GAT'ATC Cuts at: 3057 3057 Size: 7342 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4000 4000 Size: 7342 FspI TGC'GCA Cuts at: 5848 5848 Size: 7342 HaeII r_GCGC'y Cuts at: 1678 4562 6489 1678 Size: 2884 1927 2531 Fragments arranged by size: 2884 2531 1927 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2012 2044 2162 2194 3236 Size: 32 135 32 440 32 118 32 1042 Cuts at: 3236 3268 3737 3769 3836 3868 4104 4136 4169 Size: 32 469 32 67 32 236 32 33 Cuts at: 4169 4201 5983 6015 6057 6089 1373 Size: 32 1782 32 42 32 2626 Fragments arranged by size: 2626 1782 1042 469 440 236 135 118 67 42 33 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4686 4932 669 Size: 988 281 2748 246 3079 Fragments arranged by size: 3079 2748 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7342 HpaI GTT'AAC Cuts at: 4932 4932 Size: 7342 KasI G'GCGC_C Cuts at: 4558 4558 Size: 7342 KpnI G_GTAC'C Cuts at: 4667 4667 Size: 7342 MfeI C'AATT_G Cuts at: 4941 4941 Size: 7342 MluI A'CGCG_T Cuts at: 7218 7218 Size: 7342 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6456 6640 1305 Size: 5151 184 2007 Fragments arranged by size: 5151 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4319 4599 10 Size: 54 1425 343 2487 280 2753 Fragments arranged by size: 2753 2487 1425 343 280 54 MslI CAynn'nnrTG Cuts at: 518 1166 2631 4625 5300 5659 5818 518 Size: 648 1465 1994 675 359 159 2042 Fragments arranged by size: 2042 1994 1465 675 648 359 159 MspA1I CmG'CkG Cuts at: 1606 4758 5435 6583 6828 1606 Size: 3152 677 1148 245 2120 Fragments arranged by size: 3152 2120 1148 677 245 NarI GG'CG_CC Cuts at: 4559 4559 Size: 7342 NcoI C'CATG_G Cuts at: 513 1065 4254 4320 513 Size: 552 3189 66 3535 Fragments arranged by size: 3535 3189 552 66 NdeI CA'TA_TG Cuts at: 387 387 Size: 7342 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7342 NotI GC'GGCC_GC Cuts at: 4789 4789 Size: 7342 NsiI A_TGCA'T Cuts at: 2638 2638 Size: 7342 NspI r_CATG'y Cuts at: 1132 1229 3139 3816 4700 6245 1132 Size: 97 1910 677 884 1545 2229 Fragments arranged by size: 2229 1910 1545 884 677 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7342 PciI A'CATG_T Cuts at: 3135 6241 3135 Size: 3106 4236 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 1108 Size: 902 6440 PmeI GTTT'AAAC Cuts at: 4646 4646 Size: 7342 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5411 5443 6924 6956 5411 Size: 32 1481 32 5797 Fragments arranged by size: 5797 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3097 3097 Size: 7342 PshAI GACnn'nnGTC Cuts at: 1613 4442 1613 Size: 2829 4513 PsiI TTA'TAA Cuts at: 2465 4912 2465 Size: 2447 4895 PspOMI G'GGCC_C Cuts at: 3427 3427 Size: 7342 PstI C_TGCA'G Cuts at: 830 4543 4694 830 Size: 3713 151 3478 Fragments arranged by size: 3713 3478 151 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7342 SalI G'TCGA_C Cuts at: 1936 4684 1936 Size: 2748 4594 SbfI CC_TGCA'GG Cuts at: 4694 4694 Size: 7342 ScaI AGT'ACT Cuts at: 5590 7302 5590 Size: 1712 5630 SexAI A'CCwGG_T Cuts at: 4612 4612 Size: 7342 SfcI C'TryA_G Cuts at: 826 1045 4419 4539 4638 4690 5825 6506 6697 Size: 219 3374 120 99 52 1135 681 191 Cuts at: 6697 826 Size: 1471 Fragments arranged by size: 3374 1471 1135 681 219 191 120 99 52 SfoI GGC'GCC Cuts at: 4560 4560 Size: 7342 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7342 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7342 SmaI CCC'GGG Cuts at: 1872 4669 1872 Size: 2797 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2202 2831 4497 5446 6347 6609 Size: 197 926 259 629 1666 949 901 262 Cuts at: 6609 820 Size: 1553 Fragments arranged by size: 1666 1553 949 926 901 629 262 259 197 SnaBI TAC'GTA Cuts at: 493 5133 493 Size: 4640 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7342 SphI G_CATG'C Cuts at: 1132 4700 1132 Size: 3568 3774 SspI AAT'ATT Cuts at: 5 52 2767 3796 5266 5 Size: 47 2715 1029 1470 2081 Fragments arranged by size: 2715 2081 1470 1029 47 StuI AGG'CCT Cuts at: 1517 4567 1517 Size: 3050 4292 StyI C'CwwG_G Cuts at: 513 1065 4254 4320 4781 513 Size: 552 3189 66 461 3074 Fragments arranged by size: 3189 3074 552 461 66 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2989 4060 5369 5386 5539 5724 6169 Size: 1255 1409 1071 1309 17 153 185 445 Cuts at: 6169 325 Size: 1498 Fragments arranged by size: 1498 1409 1309 1255 1071 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2699 3929 3947 Size: 274 80 33 51 1443 722 1230 18 Cuts at: 3947 4358 5588 7300 96 Size: 411 1230 1712 138 Fragments arranged by size: 1712 1443 1230 1230 722 411 274 138 80 51 33 18 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2018 2050 7302 7334 2018 Size: 32 5252 32 2026 Fragments arranged by size: 5252 2026 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3476 1316 Size: 2160 5182 XbaI T'CTAG_A Cuts at: 2053 2728 2920 4678 2053 Size: 675 192 1758 4717 Fragments arranged by size: 4717 1758 675 192 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7342 XmaI C'CCGG_G Cuts at: 1870 4667 1870 Size: 2797 4545 XmnI GAAnn'nnTTC Cuts at: 2598 2658 3137 5471 2598 Size: 60 479 2334 4469 Fragments arranged by size: 4469 2334 479 60 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3669 5150 276 Size: 53 83 186 936 2135 1481 2468 Fragments arranged by size: 2468 2135 1481 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BsiWI EcoICRI EcoNI FseI FspAI NaeI NgoMIV NruI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SrfI SwaI XcmI