Restriction map for
pFN21AA1525
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# NO CUT ENZYMES ( 82 enzymes) #
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Acc65I AocI Aor51HI ApaI AscI
AseI AsnI Asp700I Asp718I AviII
AvrII BamHI BbrPI BbvCI BcgI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaI BsiEI BsiWI BsmI BspDI
BsrBI BssHII BssSI BstXI Bsu36I
CelII ClaI CpoI EagI EclXI
Eco47III Eco52I Eco81I EcoRI FseI
FspI HincII HindII HpaI KpnI
KspI MamI MfeI MluI MunI
NaeI NgoMIV NheI NotI NruI
PacI PaeR7I PmaCI PmeI PmlI
PshAI PshBI PvuI RsrII SacII
SalI SbfI SfiI SgrAI SmaI
SnaBI SpeI SphI Sse8387I StuI
SwaI XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 69 enzymes) #
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AatII 2988 GACGTC GACGT^C
AccIII 2826 TCCGGA T^CCGGA
AclI 2147 AACGTT AA^CGTT
AflII 1596 CTTAAG C^TTAAG
AflIII 728 ACATGT A^C[GA][CT]GT
AgeI 3145 ACCGGT A^CCGGT
AhdI 3277 GACAGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1252 GTGCAC G^TGCAC
ApaLI 1252 GTGCAC G^TGCAC
AspEI 3277 GACAGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1966 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 39 CCCGAG C^[CT]CG[GA]G
BalI 144 TGGCCA TGG^CCA
BanI 2041 GGCGCC G^G[CT][GA]CC
BbeI 2041 GGCGCC GGCGC^C
BbsI 3266 GAAGAC GAAGAC(2/6)
BciVI 884 GGATAC GTATCC(6/5)
BclI 1874 TGATCA T^GATCA
BcnI 374 CCCGG CC^[GC]GG
BfrI 1596 CTTAAG C^TTAAG
BglII 2238 AGATCT A^GATCT
BpmI 2409 CTCCAG CTGGAG(16/14)
BpuAI 3266 GAAGAC GAAGAC(2/6)
BseAI 2826 TCCGGA T^CCGGA
BsoBI 39 CCCGAG C^[CT]CG[GA]G
Bsp1407I 2930 TGTACA T^GTACA
BspEI 2826 TCCGGA T^CCGGA
BspHI 1298 TCATGA T^CATGA
BspLU11I 728 ACATGT A^CATGT
BspMI 2004 GCAGGT ACCTGC(4/8)
BsrFI 3145 ACCGGT [GA]^CCGG[CT]
BsrGI 2930 TGTACA T^GTACA
Bst1107I 996 GTATAC GTA^TAC
BstBI 863 TTCGAA TT^CGAA
BstEII 3353 GGTCACC G^GT[ACGT]ACC
BstPI 3353 GGTCACC G^GT[ACGT]ACC
BstZ17I 996 GTATAC GTA^TAC
Cfr10I 3145 ACCGGT [GA]^CCGG[CT]
DraI 499 TTTAAA TTT^AAA
DraIII 2951 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2348 GACCTCCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 144 TGGCCA [CT]^GGCC[GA]
Eam1105I 3277 GACAGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 3353 GGTCACC G^GT[ACGT]ACC
FauI 2033 GCGGG CCCGC(4/6)
FbaI 1874 TGATCA T^GATCA
HaeII 2041 GGCGCC [GA]GCGC^[CT]
KasI 2041 GGCGCC G^GCGCC
MluNI 144 TGGCCA TGG^CCA
MroI 2826 TCCGGA T^CCGGA
MscI 144 TGGCCA TGG^CCA
NarI 2041 GGCGCC GG^CGCC
NciI 374 CCCGG CC^[GC]GG
NdeI 1235 CATATG CA^TATG
NspV 863 TTCGAA TT^CGAA
PflFI 1966 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 523 CCAGAGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 3145 ACCGGT A^CCGGT
Psp1406I 2147 AACGTT AA^CGTT
PvuII 560 CAGCTG CAG^CTG
RcaI 1298 TCATGA T^CATGA
ScaI 1627 AGTACT AGT^ACT
SexAI 2979 ACCTGGT A^CC[AT]GGT
SfoI 2041 GGCGCC GGC^GCC
SfuI 863 TTCGAA TT^CGAA
SspBI 2930 TGTACA T^GTACA
SspI 438 AATATT AAT^ATT
Tth111I 1966 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 523 CCAGAGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 19 enzymes) #
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AccII 2200 CGCG CG^CG
2622 CGCG
AcyI 2041 GGCGCC G[GA]^CG[CT]C
2988 GACGTC
BsaHI 2041 GGCGCC G[GA]^CG[CT]C
2988 GACGTC
BsmBI 1948 CGTCTC CGTCTC(1/5)
2990 CGTCTC
BstAPI 1983 GCAGGAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2095 GCAGATGCTGC
BstUI 2200 CGCG CG^CG
2622 CGCG
CfoI 1794 GCGC GCG^C
2042 GCGC
EcoNI 1275 CCTCTGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2899 CCTTCGCAAGG
EcoT22I 11 ATGCAT ATGCA^T
296 ATGCAT
HgaI 2885 GACGC GACGC(5/10)
216 GCGTC
HhaI 1794 GCGC GCG^C
2042 GCGC
Hin1I 2041 GGCGCC G[GA]^CG[CT]C
2988 GACGTC
HinP1I 1794 GCGC G^CGC
2042 GCGC
HindIII 782 AAGCTT A^AGCTT
1262 AAGCTT
MspA1I 560 CAGCTG C[AC]G^C[GT]G
3103 CAGCGG
MvnI 2200 CGCG CG^CG
2622 CGCG
NsiI 11 ATGCAT ATGCA^T
296 ATGCAT
PpuMI 82 GGGACCT [GA]G^G[AT]CC[CT]
1505 GGGACCT
SapI 814 GAAGAGC GCTCTTC(1/4)
1519 GAAGAGC
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# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaII
BanII BbvI BfaI BglI BmyI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraII DsaI EarI EcoO109I EcoRII
EcoRV EcoT14I Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspI MvaI MwoI NcoI NdeII
NlaIII NlaIV NspI PleI PstI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII