Restriction map for
pFN21AA1525
(Linear) (Six-base) MAPSORT of: KIAA1525.seq Check: 1554 from: 1 to: 3396
KIAA1525.orf
With 182 enzymes: SgfI *
February 28, 2007 11:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1995 3396
Size: 1995 1401
AatII G_ACGT'C
Cuts at: 0 2992 3396
Size: 2992 404
AccI GT'mk_AC
Cuts at: 0 431 997 2444 2678 3396
Size: 431 566 1447 234 718
Fragments arranged by size:
1447 718 566 431 234
AclI AA'CG_TT
Cuts at: 0 2148 3396
Size: 2148 1248
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 746 1901 2726 3260 3396
Size: 746 1155 825 534 136
Fragments arranged by size:
1155 825 746 534 136
AflII C'TTAA_G
Cuts at: 0 1596 3396
Size: 1596 1800
AflIII A'CryG_T
Cuts at: 0 728 3396
Size: 728 2668
AgeI A'CCGG_T
Cuts at: 0 3145 3396
Size: 3145 251
AhdI GACnn_n'nnGTC
Cuts at: 0 3282 3396
Size: 3282 114
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1712 3396
Size: 1712 1684
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1370 1402 3396
Size: 1370 32 1994
Fragments arranged by size:
1994 1370 32
AlwNI CAG_nnn'CTG
Cuts at: 0 769 1989 2101 2569 2704 2733 3396
Size: 769 1220 112 468 135 29 663
Fragments arranged by size:
1220 769 663 468 135 112 29
ApaLI G'TGCA_C
Cuts at: 0 1252 3396
Size: 1252 2144
ApoI r'AATT_y
Cuts at: 0 54 676 690 867 1141 1285 1934 2060
Size: 54 622 14 177 274 144 649 126
Cuts at: 2060 2131 3396
Size: 71 1265
Fragments arranged by size:
1265 649 622 274 177 144 126 71
54 14
AvaI C'yCGr_G
Cuts at: 0 39 3396
Size: 39 3357
BanI G'GyrC_C
Cuts at: 0 2041 3396
Size: 2041 1355
BanII G_rGCy'C
Cuts at: 0 821 1526 3303 3396
Size: 821 705 1777 93
Fragments arranged by size:
1777 821 705 93
BbeI G_GCGC'C
Cuts at: 0 2045 3396
Size: 2045 1351
BbsI GAAGACnn'nnnn_
Cuts at: 0 3273 3396
Size: 3273 123
BciVI GTATCCnnnnn_n'
Cuts at: 0 878 3396
Size: 878 2518
BclI T'GATC_A
Cuts at: 0 1874 3396
Size: 1874 1522
BfrBI ATG'CAT
Cuts at: 0 13 298 3396
Size: 13 285 3098
Fragments arranged by size:
3098 285 13
BglI GCCn_nnn'nGGC
Cuts at: 0 358 478 832 3396
Size: 358 120 354 2564
Fragments arranged by size:
2564 358 354 120
BglII A'GATC_T
Cuts at: 0 2238 3396
Size: 2238 1158
Bme1580I G_kGCm'C
Cuts at: 0 1256 3396
Size: 1256 2140
BmgBI CAC'GTC
Cuts at: 0 1005 3396
Size: 1005 2391
BmrI ACTGGGnnnn_n'
Cuts at: 0 2348 2434 2506 3396
Size: 2348 86 72 890
Fragments arranged by size:
2348 890 86 72
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 283 1206 3396
Size: 283 923 2190
Fragments arranged by size:
2190 923 283
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2394 3396
Size: 2394 1002
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 618 1201 2632 3396
Size: 618 583 1431 764
Fragments arranged by size:
1431 764 618 583
BsaHI Gr'CG_yC
Cuts at: 0 2042 2989 3396
Size: 2042 947 407
Fragments arranged by size:
2042 947 407
BsaWI w'CCGG_w
Cuts at: 0 2826 3145 3259 3396
Size: 2826 319 114 137
Fragments arranged by size:
2826 319 137 114
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1038 1068 3396
Size: 1038 30 2328
Fragments arranged by size:
2328 1038 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 256 1622 1942 3396
Size: 256 1366 320 1454
Fragments arranged by size:
1454 1366 320 256
BseYI C'CCAG_C
Cuts at: 0 2301 2726 3101 3396
Size: 2301 425 375 295
Fragments arranged by size:
2301 425 375 295
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 830 2029 2450 3396
Size: 830 1199 421 946
Fragments arranged by size:
1199 946 830 421
BsiHKAI G_wGCw'C
Cuts at: 0 821 1256 1526 3303 3396
Size: 821 435 270 1777 93
Fragments arranged by size:
1777 821 435 270 93
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1954 2996 3396
Size: 1954 1042 400
Fragments arranged by size:
1954 1042 400
Bsp1286I G_dGCh'C
Cuts at: 0 821 1256 1526 3303 3396
Size: 821 435 270 1777 93
Fragments arranged by size:
1777 821 435 270 93
BspEI T'CCGG_A
Cuts at: 0 2826 3396
Size: 2826 570
BspHI T'CATG_A
Cuts at: 0 1298 3396
Size: 1298 2098
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1995 3396
Size: 1995 1401
BsrDI GCAATG_nn'
Cuts at: 0 379 1175 1613 3396
Size: 379 796 438 1783
Fragments arranged by size:
1783 796 438 379
BsrFI r'CCGG_y
Cuts at: 0 3145 3396
Size: 3145 251
BsrGI T'GTAC_A
Cuts at: 0 2930 3396
Size: 2930 466
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1989 2101 3396
Size: 1989 112 1295
Fragments arranged by size:
1989 1295 112
BstBI TT'CG_AA
Cuts at: 0 864 3396
Size: 864 2532
BstEII G'GTnAC_C
Cuts at: 0 3353 3396
Size: 3353 43
BstYI r'GATC_y
Cuts at: 0 1532 1785 2238 3256 3396
Size: 1532 253 453 1018 140
Fragments arranged by size:
1532 1018 453 253 140
BstZ17I GTA'TAC
Cuts at: 0 998 3396
Size: 998 2398
BtgI C'CryG_G
Cuts at: 0 141 1109 3294 3396
Size: 141 968 2185 102
Fragments arranged by size:
2185 968 141 102
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2604 3396
Size: 2604 792
BtsI GCAGTG_nn'
Cuts at: 0 505 1466 2486 3396
Size: 505 961 1020 910
Fragments arranged by size:
1020 961 910 505
DraI TTT'AAA
Cuts at: 0 501 3396
Size: 501 2895
DraIII CAC_nnn'GTG
Cuts at: 0 2956 3396
Size: 2956 440
DrdI GACnn_nn'nnGTC
Cuts at: 0 2354 3396
Size: 2354 1042
EaeI y'GGCC_r
Cuts at: 0 144 3396
Size: 144 3252
EarI CTCTTCn'nnn_
Cuts at: 0 809 1514 1729 3245 3396
Size: 809 705 215 1516 151
Fragments arranged by size:
1516 809 705 215 151
EcoICRI GAG'CTC
Cuts at: 0 819 1524 3301 3396
Size: 819 705 1777 95
Fragments arranged by size:
1777 819 705 95
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 746 1901 2394 2726 3260 3396
Size: 746 1155 493 332 534 136
Fragments arranged by size:
1155 746 534 493 332 136
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1279 2903 3396
Size: 1279 1624 493
Fragments arranged by size:
1624 1279 493
EcoO109I rG'GnC_Cy
Cuts at: 0 83 1506 2015 2719 3396
Size: 83 1423 509 704 677
Fragments arranged by size:
1423 704 677 509 83
EcoRV GAT'ATC
Cuts at: 0 654 1173 1386 3396
Size: 654 519 213 2010
Fragments arranged by size:
2010 654 519 213
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1405 1931 3387 3396
Size: 1405 526 1456 9
Fragments arranged by size:
1456 1405 526 9
HaeII r_GCGC'y
Cuts at: 0 2045 3396
Size: 2045 1351
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 307 339 1215 1247 1614 1646 2230 2262
Size: 307 32 876 32 367 32 584 32
Cuts at: 2262 3009 3041 3268 3300 3396
Size: 747 32 227 32 96
Fragments arranged by size:
876 747 584 367 307 227 96 32
32 32 32 32 32
HindIII A'AGCT_T
Cuts at: 0 782 1262 3396
Size: 782 480 2134
Fragments arranged by size:
2134 782 480
KasI G'GCGC_C
Cuts at: 0 2041 3396
Size: 2041 1355
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 199 3396
Size: 199 3197
MscI TGG'CCA
Cuts at: 0 146 3396
Size: 146 3250
MslI CAynn'nnrTG
Cuts at: 0 146 1235 2623 3080 3396
Size: 146 1089 1388 457 316
Fragments arranged by size:
1388 1089 457 316 146
MspA1I CmG'CkG
Cuts at: 0 562 3105 3396
Size: 562 2543 291
Fragments arranged by size:
2543 562 291
NarI GG'CG_CC
Cuts at: 0 2042 3396
Size: 2042 1354
NcoI C'CATG_G
Cuts at: 0 141 1109 3294 3396
Size: 141 968 2185 102
Fragments arranged by size:
2185 968 141 102
NdeI CA'TA_TG
Cuts at: 0 1236 3396
Size: 1236 2160
NsiI A_TGCA'T
Cuts at: 0 15 300 3396
Size: 15 285 3096
Fragments arranged by size:
3096 285 15
NspI r_CATG'y
Cuts at: 0 732 1152 3233 3396
Size: 732 420 2081 163
Fragments arranged by size:
2081 732 420 163
PasI CC'CwG_GG
Cuts at: 0 3288 3396
Size: 3288 108
PciI A'CATG_T
Cuts at: 0 728 3396
Size: 728 2668
PflMI CCAn_nnn'nTGG
Cuts at: 0 529 3396
Size: 529 2867
PpuMI rG'GwC_Cy
Cuts at: 0 83 1506 3396
Size: 83 1423 1890
Fragments arranged by size:
1890 1423 83
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1559 1591 3396
Size: 1559 32 1805
Fragments arranged by size:
1805 1559 32
PstI C_TGCA'G
Cuts at: 0 1985 2109 2223 2493 3177 3396
Size: 1985 124 114 270 684 219
Fragments arranged by size:
1985 684 270 219 124 114
PvuII CAG'CTG
Cuts at: 0 562 3396
Size: 562 2834
SacI G_AGCT'C
Cuts at: 0 821 1526 3303 3396
Size: 821 705 1777 93
Fragments arranged by size:
1777 821 705 93
SapI GCTCTTCn'nnn_
Cuts at: 0 809 1514 3396
Size: 809 705 1882
Fragments arranged by size:
1882 809 705
ScaI AGT'ACT
Cuts at: 0 1629 3396
Size: 1629 1767
SexAI A'CCwGG_T
Cuts at: 0 2979 3396
Size: 2979 417
SfcI C'TryA_G
Cuts at: 0 1029 1366 1981 2105 2219 2489 2675 3173
Size: 1029 337 615 124 114 270 186 498
Cuts at: 3173 3396
Size: 223
Fragments arranged by size:
1029 615 498 337 270 223 186 124
114
SfoI GGC'GCC
Cuts at: 0 2043 3396
Size: 2043 1353
SmlI C'TyrA_G
Cuts at: 0 633 1216 1596 2647 3396
Size: 633 583 380 1051 749
Fragments arranged by size:
1051 749 633 583 380
SspI AAT'ATT
Cuts at: 0 440 3396
Size: 440 2956
StyI C'CwwG_G
Cuts at: 0 141 1109 3294 3396
Size: 141 968 2185 102
Fragments arranged by size:
2185 968 141 102
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 263 926 1239 2617 3396
Size: 263 663 313 1378 779
Fragments arranged by size:
1378 779 663 313 263
TatI w'GTAC_w
Cuts at: 0 410 421 1627 2209 2930 3396
Size: 410 11 1206 582 721 466
Fragments arranged by size:
1206 721 582 466 410 11
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1038 1070 3396
Size: 1038 32 2326
Fragments arranged by size:
2326 1038 32
Tth111I GACn'n_nGTC
Cuts at: 0 1969 3396
Size: 1969 1427
ZraI GAC'GTC
Cuts at: 0 2990 3396
Size: 2990 406
Enzymes that do cut:
AarI AatII AccI AclI AcuI AflII AflIII AgeI
AhdI AlfI AloI AlwNI ApaLI ApoI AvaI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglI BglII
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrDI BsrFI BsrGI BstAPI BstBI BstEII
BstYI BstZ17I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI
HaeII Hin4I HindIII KasI MmeI MscI MslI MspA1I
NarI NcoI NdeI NsiI NspI PasI PciI PflMI
PpuMI PsrI PstI PvuII SacI SapI ScaI SexAI
SfcI SfoI SmlI SspI StyI TaqII TatI TstI
Tth111I ZraI
Enzymes that do not cut:
Acc65I AfeI AleI ApaI AscI AseI AsiSI AvrII
BaeI BamHI BbvCI BcgI BlpI BmtI Bpu10I BsaI
BsaAI BsaBI BsiEI BsiWI BsmI BsrBI BssHII BssSI
BstXI Bsu36I ClaI CspCI EagI EciI EcoRI FseI
FspI FspAI HincII HpaI KpnI MfeI MluI NaeI
NgoMIV NheI NotI NruI PacI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PvuI RsrII SacII
SalI SanDI SbfI SfiI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI StuI SwaI XbaI XcmI XhoI
XmaI XmnI