(Circular) (Six-base) MAPSORT of: pFN21AA1462.seq Check: 8254 from: 1 to: 8782 pFN21AA1462 8782 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3665 3665 Size: 8782 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2183 6592 278 Size: 53 83 186 936 647 4409 2468 Fragments arranged by size: 4409 2468 936 647 186 83 53 AccI GT'mk_AC Cuts at: 1937 6125 1937 Size: 4188 4594 Acc65I G'GTAC_C Cuts at: 6103 6103 Size: 8782 AclI AA'CG_TT Cuts at: 1563 6909 7282 1563 Size: 5346 373 3063 Fragments arranged by size: 5346 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3150 5623 5815 6845 8229 8497 1632 Size: 1518 2473 192 1030 1384 268 1917 Fragments arranged by size: 2473 1917 1518 1384 1030 268 192 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8585 AflIII A'CryG_T Cuts at: 1203 2197 2659 7681 8658 1203 Size: 994 462 5022 977 1327 Fragments arranged by size: 5022 1327 994 977 462 AgeI A'CCGG_T Cuts at: 4056 4217 4471 7613 4056 Size: 161 254 3142 5225 Fragments arranged by size: 5225 3142 254 161 AhdI GACnn_n'nnGTC Cuts at: 5149 7511 5149 Size: 2362 6420 AleI CACnn'nnGTG Cuts at: 1166 4779 1166 Size: 3613 5169 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4855 5374 1184 Size: 3671 519 4592 Fragments arranged by size: 4592 3671 519 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5585 5617 5585 Size: 32 8750 AlwNI CAG_nnn'CTG Cuts at: 2131 2537 3002 3874 4178 5564 5690 5771 6525 Size: 406 465 872 304 1386 126 81 754 Cuts at: 6525 8097 2131 Size: 1572 2816 Fragments arranged by size: 2816 1572 1386 872 754 465 406 304 126 81 ApaI G_GGCC'C Cuts at: 4430 4430 Size: 8782 ApaLI G'TGCA_C Cuts at: 4830 6839 7995 4830 Size: 2009 1156 5617 Fragments arranged by size: 5617 2009 1156 ApoI r'AATT_y Cuts at: 1493 2668 2739 2806 2824 6091 6317 6469 8578 Size: 1175 71 67 18 3267 226 152 2109 Cuts at: 8578 1493 Size: 1697 Fragments arranged by size: 3267 2109 1697 1175 226 152 71 67 18 AseI AT'TA_AT Cuts at: 160 1032 7336 160 Size: 872 6304 1606 Fragments arranged by size: 6304 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8782 AvaI C'yCGr_G Cuts at: 1870 1943 2717 3518 4798 5899 6107 1870 Size: 73 774 801 1280 1101 208 4545 Fragments arranged by size: 4545 1280 1101 801 774 208 73 AvrII C'CTAG_G Cuts at: 4991 5756 4991 Size: 765 8017 BamHI G'GATC_C Cuts at: 5268 6112 6479 5268 Size: 844 367 7571 Fragments arranged by size: 7571 844 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2091 3088 3419 4053 4324 Size: 325 217 627 304 997 331 634 271 Cuts at: 4324 4696 5183 6042 6103 7558 618 Size: 372 487 859 61 1455 1842 Fragments arranged by size: 1842 1455 997 859 634 627 487 372 331 325 304 271 217 61 BanII G_rGCy'C Cuts at: 1391 2250 2988 3206 3225 3854 4101 4384 4422 Size: 859 738 218 19 629 247 283 38 Cuts at: 4422 4430 4530 4709 5001 5100 5133 5163 5346 Size: 8 100 179 292 99 33 30 183 Cuts at: 5346 5474 5512 5583 5804 5898 6101 1391 Size: 128 38 71 221 94 203 4072 Fragments arranged by size: 4072 859 738 629 292 283 247 221 218 203 183 179 128 100 99 94 71 38 38 33 30 19 8 BbeI G_GCGC'C Cuts at: 2095 2095 Size: 8782 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2025 3087 3342 3625 3879 3981 4932 Size: 971 126 1062 255 283 254 102 951 Cuts at: 4932 928 Size: 4778 Fragments arranged by size: 4778 1062 971 951 283 255 254 126 102 BbvCI CC'TCA_GC Cuts at: 2305 4271 2305 Size: 1966 6816 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5336 5370 6973 7007 1903 Size: 34 3399 34 1603 34 3678 Fragments arranged by size: 3678 3399 1603 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2033 2796 6674 7884 2033 Size: 763 3878 1210 2931 Fragments arranged by size: 3878 2931 1210 763 BclI T'GATC_A Cuts at: 1548 3826 4222 4497 1548 Size: 2278 396 275 5833 Fragments arranged by size: 5833 2278 396 275 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3220 5507 7393 Size: 107 122 71 631 605 1548 2287 1886 Cuts at: 7393 136 Size: 1525 Fragments arranged by size: 2287 1886 1548 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3955 8777 3955 Size: 4822 3960 BlpI GC'TnA_GC Cuts at: 2845 3315 3878 4843 5340 6199 2845 Size: 470 563 965 497 859 5428 Fragments arranged by size: 5428 965 859 563 497 470 Bme1580I G_kGCm'C Cuts at: 1790 3093 3612 4056 4246 4430 4834 5938 6047 Size: 1303 519 444 190 184 404 1104 109 Cuts at: 6047 6843 7999 1790 Size: 796 1156 2573 Fragments arranged by size: 2573 1303 1156 1104 796 519 444 404 190 184 109 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8782 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2118 3192 3702 Size: 416 53 83 399 24 701 1074 510 Cuts at: 3702 3755 3813 6513 7471 442 Size: 53 58 2700 958 1753 Fragments arranged by size: 2700 1753 1074 958 701 510 416 399 83 58 53 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8782 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3873 5818 3873 Size: 1945 6837 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2426 3000 3352 3920 4514 4771 4901 5124 Size: 1187 574 352 568 594 257 130 223 Cuts at: 5124 5965 7442 1239 Size: 841 1477 2579 Fragments arranged by size: 2579 1477 1187 841 594 574 568 352 257 223 130 Bpu10I CC'TnA_GC Cuts at: 2305 4271 4395 5571 5577 5825 2305 Size: 1966 124 1176 6 248 5262 Fragments arranged by size: 5262 1966 1176 248 124 6 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2867 3369 4013 4282 6907 7772 8070 2867 Size: 502 644 269 2625 865 298 3579 Fragments arranged by size: 3579 2625 865 644 502 298 269 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2049 3549 7445 882 Size: 617 550 1500 3896 2219 Fragments arranged by size: 3896 2219 1500 617 550 BsaAI yAC'GTr Cuts at: 493 1204 2449 3025 4781 6573 493 Size: 711 1245 576 1756 1792 2702 Fragments arranged by size: 2702 1792 1756 1245 711 576 BsaBI GATnn'nnATC Cuts at: 8776 8776 Size: 8782 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2092 2180 2964 4331 Size: 53 83 186 936 559 88 784 1367 Cuts at: 4331 5856 6589 6971 7606 275 Size: 1525 733 382 635 1451 Fragments arranged by size: 1525 1451 1367 936 784 733 635 559 382 186 88 83 53 BsaWI w'CCGG_w Cuts at: 2349 4056 4217 4471 5313 7215 7613 7887 8034 Size: 1707 161 254 842 1902 398 274 147 Cuts at: 8034 8445 2349 Size: 411 2686 Fragments arranged by size: 2686 1902 1707 842 411 398 274 254 161 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2270 2300 2609 2639 2646 Size: 30 165 30 867 30 309 30 7 Cuts at: 2646 2676 5585 5615 5895 5925 5963 5993 1178 Size: 30 2909 30 280 30 38 30 3967 Fragments arranged by size: 3967 2909 867 309 280 165 38 30 30 30 30 30 30 30 30 7 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2937 4109 4598 4772 5502 1186 Size: 1751 1172 489 174 730 4466 Fragments arranged by size: 4466 1751 1172 730 489 174 BseYI C'CCAG_C Cuts at: 2062 3143 4132 5398 5582 7985 8481 2062 Size: 1081 989 1266 184 2403 496 2363 Fragments arranged by size: 2403 2363 1266 1081 989 496 184 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4170 4884 5344 1163 Size: 573 2434 714 460 4601 Fragments arranged by size: 4601 2434 714 573 460 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3135 6232 6993 8021 1540 Size: 275 186 1134 3097 761 1028 2301 Fragments arranged by size: 3097 2301 1134 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2258 2397 2988 3656 4530 4621 4834 5529 5804 Size: 139 591 668 874 91 213 695 275 Cuts at: 5804 6843 6928 7999 2258 Size: 1039 85 1071 3041 Fragments arranged by size: 3041 1071 1039 874 695 668 591 275 213 139 91 85 BsmI GAATG_Cn' Cuts at: 3411 4033 5842 6293 6386 3411 Size: 622 1809 451 93 5807 Fragments arranged by size: 5807 1809 622 451 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2250 2258 2397 2988 3093 3206 3225 Size: 399 460 8 139 591 105 113 19 Cuts at: 3225 3612 3656 3854 4056 4101 4246 4384 4422 Size: 387 44 198 202 45 145 138 38 Cuts at: 4422 4430 4530 4621 4709 4834 5001 5100 5133 Size: 8 100 91 88 125 167 99 33 Cuts at: 5133 5163 5346 5474 5512 5529 5583 5804 5898 Size: 30 183 128 38 17 54 221 94 Cuts at: 5898 5938 6047 6101 6843 6928 7999 1391 Size: 40 109 54 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 591 460 399 387 221 202 198 183 167 145 139 138 128 125 113 109 105 100 99 94 91 88 85 54 54 45 44 40 38 38 33 30 19 17 8 8 BspEI T'CCGG_A Cuts at: 5313 8445 5313 Size: 3132 5650 BspHI T'CATG_A Cuts at: 6671 8401 6671 Size: 1730 7052 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3665 4901 6137 844 Size: 2821 1236 1236 3489 Fragments arranged by size: 3489 2821 1236 1236 BsrBI CCG'CTC Cuts at: 1394 1823 2927 5321 6669 1394 Size: 429 1104 2394 1348 3507 Fragments arranged by size: 3507 2394 1348 1104 429 BsrDI GCAATG_nn' Cuts at: 65 1248 7277 7451 65 Size: 1183 6029 174 1396 Fragments arranged by size: 6029 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3391 4056 4217 4471 5404 7426 7613 Size: 110 1581 665 161 254 933 2022 187 Cuts at: 7613 1700 Size: 2869 Fragments arranged by size: 2869 2022 1581 933 665 254 187 161 110 BsrGI T'GTAC_A Cuts at: 96 2007 96 Size: 1911 6871 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8782 BssSI C'ACGA_G Cuts at: 2115 2818 4858 6842 7854 2115 Size: 703 2040 1984 1012 3043 Fragments arranged by size: 3043 2040 1984 1012 703 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8782 BstBI TT'CG_AA Cuts at: 5607 7586 5607 Size: 1979 6803 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8782 BstXI CCAn_nnnn'nTGG Cuts at: 2419 3898 4376 4785 5590 2419 Size: 1479 478 409 805 5611 Fragments arranged by size: 5611 1479 805 478 409 BstYI r'GATC_y Cuts at: 1972 3955 5268 6112 6479 6864 6881 8333 8419 Size: 1983 1313 844 367 385 17 1452 86 Cuts at: 8419 8431 8777 1972 Size: 12 346 1977 Fragments arranged by size: 1983 1977 1452 1313 844 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3216 5514 3216 Size: 2298 6484 BtgI C'CryG_G Cuts at: 513 1065 2184 2239 2462 3290 4314 4369 4422 Size: 552 1119 55 223 828 1024 55 53 Cuts at: 4422 4648 513 Size: 226 4647 Fragments arranged by size: 4647 1119 1024 828 552 226 223 55 55 53 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 8043 Fragments arranged by size: 8043 683 56 BtsI GCAGTG_nn' Cuts at: 2696 2941 3246 3378 4585 4621 4964 5929 6298 Size: 245 305 132 1207 36 343 965 369 Cuts at: 6298 7092 7112 2696 Size: 794 20 4366 Fragments arranged by size: 4366 1207 965 794 369 343 305 245 132 36 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8782 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8747 DraI TTT'AAA Cuts at: 3307 6086 3307 Size: 2779 6003 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2969 4309 4936 5546 7789 809 Size: 507 1653 1340 627 610 2243 1802 Fragments arranged by size: 2243 1802 1653 1340 627 610 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3480 4046 4771 Size: 54 1425 50 275 18 1650 566 725 Cuts at: 4771 6229 7118 8747 8 Size: 1458 889 1629 43 Fragments arranged by size: 1650 1629 1458 1425 889 725 566 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6229 1537 Size: 275 4417 4090 Fragments arranged by size: 4417 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2350 2828 4530 4720 5376 5982 6014 6712 Size: 997 478 1702 190 656 606 32 698 Cuts at: 6712 1353 Size: 3423 Fragments arranged by size: 3423 1702 997 698 656 606 478 190 32 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3021 4610 7369 7743 7889 387 Size: 1064 138 1432 1589 2759 374 146 1280 Fragments arranged by size: 2759 1589 1432 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2986 4528 5802 2986 Size: 1542 1274 5966 Fragments arranged by size: 5966 1542 1274 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2426 3000 3150 3352 3920 4514 4771 Size: 393 794 574 150 202 568 594 257 Cuts at: 4771 4901 5124 5623 5815 5965 6845 7442 8229 Size: 130 223 499 192 150 880 597 787 Cuts at: 8229 8497 1239 Size: 268 1524 Fragments arranged by size: 1524 880 794 787 597 594 574 568 499 393 268 257 223 202 192 150 150 130 EcoNI CCTnn'n_nnAGG Cuts at: 3165 3165 Size: 8782 EcoO109I rG'GnC_Cy Cuts at: 2128 2534 2877 3170 3910 4266 4427 2128 Size: 406 343 293 740 356 161 6483 Fragments arranged by size: 6483 740 406 356 343 293 161 EcoRI G'AATT_C Cuts at: 2668 2824 6091 6469 2668 Size: 156 3267 378 4981 Fragments arranged by size: 4981 3267 378 156 FspI TGC'GCA Cuts at: 2800 7288 2800 Size: 4488 4294 HaeII r_GCGC'y Cuts at: 1678 2095 7929 1678 Size: 417 5834 2531 Fragments arranged by size: 5834 2531 417 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2443 2475 3341 3373 3459 Size: 32 135 32 871 32 866 32 86 Cuts at: 3459 3491 4724 4756 5135 5167 5375 5407 5963 Size: 32 1233 32 379 32 208 32 556 Cuts at: 5963 5995 6013 6045 7423 7455 7497 7529 1373 Size: 32 18 32 1378 32 42 32 2626 Fragments arranged by size: 2626 1378 1233 871 866 556 379 208 135 86 42 32 32 32 32 32 32 32 32 32 32 32 32 18 HincII GTy'rAC Cuts at: 669 1657 1938 3119 6126 6372 669 Size: 988 281 1181 3007 246 3079 Fragments arranged by size: 3079 3007 1181 988 281 246 HindIII A'AGCT_T Cuts at: 748 3344 748 Size: 2596 6186 HpaI GTT'AAC Cuts at: 6372 6372 Size: 8782 KasI G'GCGC_C Cuts at: 2091 2091 Size: 8782 KpnI G_GTAC'C Cuts at: 6107 6107 Size: 8782 MfeI C'AATT_G Cuts at: 6381 6381 Size: 8782 MluI A'CGCG_T Cuts at: 8658 8658 Size: 8782 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2452 3155 7896 8080 1305 Size: 1147 703 4741 184 2007 Fragments arranged by size: 4741 2007 1147 703 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4048 4773 10 Size: 54 1425 343 2216 725 4019 Fragments arranged by size: 4019 2216 1425 725 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2148 2202 3098 4779 5282 5532 6740 Size: 648 982 54 896 1681 503 250 1208 Cuts at: 6740 7099 7258 518 Size: 359 159 2042 Fragments arranged by size: 2042 1681 1208 982 896 648 503 359 250 159 54 MspA1I CmG'CkG Cuts at: 1606 2186 3153 3561 4114 4650 4985 6198 6875 Size: 580 967 408 553 536 335 1213 677 Cuts at: 6875 8023 8268 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1213 1148 967 677 580 553 536 408 335 245 NaeI GCC'GGC Cuts at: 3393 3393 Size: 8782 NarI GG'CG_CC Cuts at: 2092 2092 Size: 8782 NcoI C'CATG_G Cuts at: 513 1065 2462 513 Size: 552 1397 6833 Fragments arranged by size: 6833 1397 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8782 NgoMIV G'CCGG_C Cuts at: 3391 3391 Size: 8782 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8782 NotI GC'GGCC_GC Cuts at: 6229 6229 Size: 8782 NspI r_CATG'y Cuts at: 1132 1229 2201 2492 2663 3886 6140 7685 1132 Size: 97 972 291 171 1223 2254 1545 2229 Fragments arranged by size: 2254 2229 1545 1223 972 291 171 97 PasI CC'CwG_GG Cuts at: 1348 3322 4388 4488 5168 5418 1348 Size: 1974 1066 100 680 250 4712 Fragments arranged by size: 4712 1974 1066 680 250 100 PciI A'CATG_T Cuts at: 2197 2659 7681 2197 Size: 462 5022 3298 Fragments arranged by size: 5022 3298 462 PflMI CCAn_nnn'nTGG Cuts at: 1108 2959 5944 1108 Size: 1851 2985 3946 Fragments arranged by size: 3946 2985 1851 PfoI T'CCnGG_A Cuts at: 4530 4530 Size: 8782 PmeI GTTT'AAAC Cuts at: 6086 6086 Size: 8782 PmlI CAC'GTG Cuts at: 2449 4781 2449 Size: 2332 6450 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5585 5617 6851 6883 8364 8396 5585 Size: 32 1234 32 1481 32 5971 Fragments arranged by size: 5971 1481 1234 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2534 4266 2534 Size: 1732 7050 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8782 PsiI TTA'TAA Cuts at: 6352 6352 Size: 8782 PspOMI G'GGCC_C Cuts at: 4426 4426 Size: 8782 PstI C_TGCA'G Cuts at: 830 2136 3909 4628 4767 6134 830 Size: 1306 1773 719 139 1367 3478 Fragments arranged by size: 3478 1773 1367 1306 719 139 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8782 PvuII CAG'CTG Cuts at: 4114 4985 4114 Size: 871 7911 SacI G_AGCT'C Cuts at: 2988 4530 5804 2988 Size: 1542 1274 5966 Fragments arranged by size: 5966 1542 1274 SacII CC_GC'GG Cuts at: 2187 4651 2187 Size: 2464 6318 SalI G'TCGA_C Cuts at: 1936 6124 1936 Size: 4188 4594 SapI GCTCTTCn'nnn_ Cuts at: 6014 6014 Size: 8782 SbfI CC_TGCA'GG Cuts at: 6134 6134 Size: 8782 ScaI AGT'ACT Cuts at: 7030 8742 7030 Size: 1712 7070 SfcI C'TryA_G Cuts at: 826 1045 2132 3905 4624 4763 6130 7265 7946 Size: 219 1087 1773 719 139 1367 1135 681 Cuts at: 7946 8137 826 Size: 191 1471 Fragments arranged by size: 1773 1471 1367 1135 1087 719 681 219 191 139 SfoI GGC'GCC Cuts at: 2093 2093 Size: 8782 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8782 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8782 SmaI CCC'GGG Cuts at: 1872 3520 4800 5901 6109 1872 Size: 1648 1280 1101 208 4545 Fragments arranged by size: 4545 1648 1280 1101 208 SmlI C'TyrA_G Cuts at: 820 1017 1943 2882 3384 4028 4297 6886 7787 Size: 197 926 939 502 644 269 2589 901 Cuts at: 7787 8049 820 Size: 262 1553 Fragments arranged by size: 2589 1553 939 926 901 644 502 269 262 197 SnaBI TAC'GTA Cuts at: 493 6573 493 Size: 6080 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8782 SphI G_CATG'C Cuts at: 1132 6140 1132 Size: 5008 3774 SspI AAT'ATT Cuts at: 5 52 6706 5 Size: 47 6654 2081 Fragments arranged by size: 6654 2081 47 StuI AGG'CCT Cuts at: 1517 3471 5907 1517 Size: 1954 2436 4392 Fragments arranged by size: 4392 2436 1954 StyI C'CwwG_G Cuts at: 513 1065 2085 2462 2579 4991 5589 5756 5967 Size: 552 1020 377 117 2412 598 167 211 Cuts at: 5967 6221 513 Size: 254 3074 Fragments arranged by size: 3074 2412 1020 598 552 377 254 211 167 117 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3149 3917 4684 4791 5198 6809 6826 Size: 1255 1569 768 767 107 407 1611 17 Cuts at: 6826 6979 7164 7609 325 Size: 153 185 445 1498 Fragments arranged by size: 1611 1569 1498 1255 768 767 445 407 185 153 107 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2007 3270 7028 Size: 274 80 33 51 1443 30 1263 3758 Cuts at: 7028 8740 96 Size: 1712 138 Fragments arranged by size: 3758 1712 1443 1263 274 138 80 51 33 30 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2646 2678 2725 2757 5895 5927 8742 8774 2646 Size: 32 47 32 3138 32 2815 32 2654 Fragments arranged by size: 3138 2815 2654 47 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 5564 1316 Size: 4248 4534 XbaI T'CTAG_A Cuts at: 6118 6118 Size: 8782 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8782 XmaI C'CCGG_G Cuts at: 1870 3518 4798 5899 6107 1870 Size: 1648 1280 1101 208 4545 Fragments arranged by size: 4545 1648 1280 1101 208 XmnI GAAnn'nnTTC Cuts at: 4939 5710 6911 4939 Size: 771 1201 6810 Fragments arranged by size: 6810 1201 771 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2181 6590 276 Size: 53 83 186 936 647 4409 2468 Fragments arranged by size: 4409 2468 936 647 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI BaeI BfrBI BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PspXI PsrI RsrII SanDI SexAI SfiI SrfI SwaI XcmI