(Circular) (Six-base) MAPSORT of: pFN21AA1457.seq Check: 7295 from: 1 to: 8734 pFN21AA1457 8734 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 5598 5871 5598 Size: 273 8461 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6544 278 Size: 53 83 186 936 5008 2468 Fragments arranged by size: 5008 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3942 4269 5226 6077 1937 Size: 2005 327 957 851 4594 Fragments arranged by size: 4594 2005 957 851 327 Acc65I G'GTAC_C Cuts at: 6055 6055 Size: 8734 AclI AA'CG_TT Cuts at: 1563 6861 7234 1563 Size: 5298 373 3063 Fragments arranged by size: 5298 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 5299 5585 5771 6797 8181 8449 1632 Size: 3667 286 186 1026 1384 268 1917 Fragments arranged by size: 3667 1917 1384 1026 286 268 186 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8537 AflIII A'CryG_T Cuts at: 1203 2376 3366 5259 7633 8610 1203 Size: 1173 990 1893 2374 977 1327 Fragments arranged by size: 2374 1893 1327 1173 990 977 AgeI A'CCGG_T Cuts at: 7565 7565 Size: 8734 AhdI GACnn_n'nnGTC Cuts at: 3799 7463 3799 Size: 3664 5070 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8734 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3018 3847 3850 5819 1184 Size: 1834 829 3 1969 4099 Fragments arranged by size: 4099 1969 1834 829 3 AlwNI CAG_nnn'CTG Cuts at: 3247 4827 5044 5413 6477 8049 3247 Size: 1580 217 369 1064 1572 3932 Fragments arranged by size: 3932 1580 1572 1064 369 217 ApaI G_GGCC'C Cuts at: 2517 5070 5898 6029 2517 Size: 2553 828 131 5222 Fragments arranged by size: 5222 2553 828 131 ApaLI G'TGCA_C Cuts at: 3389 5108 5597 6791 7947 3389 Size: 1719 489 1194 1156 4176 Fragments arranged by size: 4176 1719 1194 1156 489 ApoI r'AATT_y Cuts at: 1493 2321 6043 6269 6421 8530 1493 Size: 828 3722 226 152 2109 1697 Fragments arranged by size: 3722 2109 1697 828 226 152 AseI AT'TA_AT Cuts at: 160 1032 7288 160 Size: 872 6256 1606 Fragments arranged by size: 6256 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8734 AvaI C'yCGr_G Cuts at: 1870 1943 3805 4537 6059 1870 Size: 73 1862 732 1522 4545 Fragments arranged by size: 4545 1862 1522 732 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 5145 5178 5145 Size: 33 8701 BamHI G'GATC_C Cuts at: 6064 6431 6064 Size: 367 8367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3176 3299 3317 3430 3921 Size: 325 217 627 1389 123 18 113 491 Cuts at: 3921 4133 4182 4595 6055 7510 618 Size: 212 49 413 1460 1455 1842 Fragments arranged by size: 1842 1460 1455 1389 627 491 413 325 217 212 123 113 49 18 BanII G_rGCy'C Cuts at: 1391 2517 2674 2814 2856 2865 2886 3026 3107 Size: 1126 157 140 42 9 21 140 81 Cuts at: 3107 3137 4089 5070 5898 6029 6053 1391 Size: 30 952 981 828 131 24 4072 Fragments arranged by size: 4072 1126 981 952 828 157 140 140 131 81 42 30 24 21 9 BbeI G_GCGC'C Cuts at: 3321 4599 3321 Size: 1278 7456 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2548 3153 4220 5305 928 Size: 971 649 605 1067 1085 4357 Fragments arranged by size: 4357 1085 1067 971 649 605 BbvCI CC'TCA_GC Cuts at: 3479 4076 3479 Size: 597 8137 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5287 5321 6925 6959 1903 Size: 34 3350 34 1604 34 3678 Fragments arranged by size: 3678 3350 1604 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4018 5146 6626 7836 4018 Size: 1128 1480 1210 4916 Fragments arranged by size: 4916 1480 1210 1128 BclI T'GATC_A Cuts at: 1548 1548 Size: 8734 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2163 3765 4519 Size: 107 122 71 631 605 491 1602 754 Cuts at: 4519 4781 5741 5829 5893 7345 136 Size: 262 960 88 64 1452 1525 Fragments arranged by size: 1602 1525 1452 960 754 631 605 491 262 122 107 88 71 64 BglII A'GATC_T Cuts at: 5664 8729 5664 Size: 3065 5669 BlpI GC'TnA_GC Cuts at: 2714 6151 2714 Size: 3437 5297 Bme1580I G_kGCm'C Cuts at: 1790 2451 2517 3181 3393 3435 4138 4187 4616 Size: 661 66 664 212 42 703 49 429 Cuts at: 4616 5070 5112 5300 5601 5898 6029 6795 7951 Size: 454 42 188 301 297 131 766 1156 Cuts at: 7951 1790 Size: 2573 Fragments arranged by size: 2573 1156 766 703 664 661 454 429 301 297 212 188 131 66 49 42 42 BmgBI CAC'GTC Cuts at: 1318 2377 4843 5392 5626 1318 Size: 1059 2466 549 234 4426 Fragments arranged by size: 4426 2466 1059 549 234 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2614 2773 3565 Size: 416 53 83 399 24 1197 159 792 Cuts at: 3565 4837 5100 6465 7423 442 Size: 1272 263 1365 958 1753 Fragments arranged by size: 1753 1365 1272 1197 958 792 416 399 263 159 83 53 24 BmtI G_CTAG'C Cuts at: 1056 5343 1056 Size: 4287 4447 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2341 4108 2341 Size: 1767 6967 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2153 2382 2759 3184 3886 3929 4331 4463 Size: 914 229 377 425 702 43 402 132 Cuts at: 4463 4547 4870 6026 7394 1239 Size: 84 323 1156 1368 2579 Fragments arranged by size: 2579 1368 1156 914 702 425 402 377 323 229 132 84 43 Bpu10I CC'TnA_GC Cuts at: 3479 3926 4076 4431 3479 Size: 447 150 355 7782 Fragments arranged by size: 7782 447 355 150 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2888 4228 6859 7724 8022 2888 Size: 1340 2631 865 298 3600 Fragments arranged by size: 3600 2631 1340 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3475 4406 4852 5247 7397 882 Size: 617 1976 931 446 395 2150 2219 Fragments arranged by size: 2219 2150 1976 931 617 446 395 BsaAI yAC'GTr Cuts at: 493 1204 3274 4972 5434 6525 493 Size: 711 2070 1698 462 1091 2702 Fragments arranged by size: 2702 2070 1698 1091 711 462 BsaBI GATnn'nnATC Cuts at: 4658 5669 8728 4658 Size: 1011 3059 4664 Fragments arranged by size: 4664 3059 1011 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3318 3456 4596 4773 Size: 53 83 186 936 1785 138 1140 177 Cuts at: 4773 6541 6923 7558 275 Size: 1768 382 635 1451 Fragments arranged by size: 1785 1768 1451 1140 936 635 382 186 177 138 83 53 BsaWI w'CCGG_w Cuts at: 2645 5001 7167 7565 7839 7986 8397 2645 Size: 2356 2166 398 274 147 411 2982 Fragments arranged by size: 2982 2356 2166 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3317 3347 4832 4862 1178 Size: 30 165 30 1914 30 1485 30 5050 Fragments arranged by size: 5050 1914 1485 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2063 2276 2552 2813 3553 4012 4594 1186 Size: 877 213 276 261 740 459 582 5326 Fragments arranged by size: 5326 877 740 582 459 276 261 213 BseYI C'CCAG_C Cuts at: 2064 2213 2505 2864 3329 3398 4189 4225 4381 Size: 149 292 359 465 69 791 36 156 Cuts at: 4381 5121 5484 5883 5888 5897 5979 7937 8433 Size: 740 363 399 5 9 82 1958 496 Cuts at: 8433 2064 Size: 2365 Fragments arranged by size: 2365 1958 791 740 496 465 399 363 359 292 156 149 82 69 36 9 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3237 3275 4439 5570 1163 Size: 573 1501 38 1164 1131 4327 Fragments arranged by size: 4327 1501 1164 1131 573 38 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2144 2942 4253 5973 6184 6945 Size: 275 186 143 798 1311 1720 211 761 Cuts at: 6945 7973 1540 Size: 1028 2301 Fragments arranged by size: 2301 1720 1311 1028 798 761 275 211 186 143 BsiHKAI G_wGCw'C Cuts at: 2814 3026 3107 3393 4563 5112 5601 6795 6880 Size: 212 81 286 1170 549 489 1194 85 Cuts at: 6880 7951 2814 Size: 1071 3597 Fragments arranged by size: 3597 1194 1170 1071 549 489 286 212 85 81 BsmI GAATG_Cn' Cuts at: 2278 3826 6245 6338 2278 Size: 1548 2419 93 4674 Fragments arranged by size: 4674 2419 1548 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2836 2940 2836 Size: 104 8630 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2451 2517 2674 2814 2856 2865 2886 Size: 399 661 66 157 140 42 9 21 Cuts at: 2886 3026 3107 3137 3181 3393 3435 4089 4138 Size: 140 81 30 44 212 42 654 49 Cuts at: 4138 4187 4563 4616 5070 5112 5300 5601 5898 Size: 49 376 53 454 42 188 301 297 Cuts at: 5898 6029 6053 6795 6880 7951 1391 Size: 131 24 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 661 654 454 399 376 301 297 212 188 157 140 140 131 85 81 66 53 49 49 44 42 42 42 30 24 21 9 BspEI T'CCGG_A Cuts at: 8397 8397 Size: 8734 BspHI T'CATG_A Cuts at: 4869 5115 6623 8353 4869 Size: 246 1508 1730 5250 Fragments arranged by size: 5250 1730 1508 246 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2542 3655 3804 4134 5043 5598 5871 6089 Size: 1698 1113 149 330 909 555 273 218 Cuts at: 6089 844 Size: 3489 Fragments arranged by size: 3489 1698 1113 909 555 330 273 218 149 BsrBI CCG'CTC Cuts at: 1394 1823 2226 5777 5914 6621 1394 Size: 429 403 3551 137 707 3507 Fragments arranged by size: 3551 3507 707 429 403 137 BsrDI GCAATG_nn' Cuts at: 65 1248 2881 7229 7403 65 Size: 1183 1633 4348 174 1396 Fragments arranged by size: 4348 1633 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2139 2676 3733 4252 5442 5679 5904 Size: 110 329 537 1057 519 1190 237 225 Cuts at: 5904 5972 7378 7565 1700 Size: 68 1406 187 2869 Fragments arranged by size: 2869 1406 1190 1057 537 519 329 237 225 187 110 68 BsrGI T'GTAC_A Cuts at: 96 2070 5634 96 Size: 1974 3564 3196 Fragments arranged by size: 3564 3196 1974 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8734 BssSI C'ACGA_G Cuts at: 3050 5909 6794 7806 3050 Size: 2859 885 1012 3978 Fragments arranged by size: 3978 2859 1012 885 BstAPI GCAn_nnn'nTGC Cuts at: 794 3710 794 Size: 2916 5818 BstBI TT'CG_AA Cuts at: 7538 7538 Size: 8734 BstEII G'GTnAC_C Cuts at: 1184 5080 5161 1184 Size: 3896 81 4757 Fragments arranged by size: 4757 3896 81 BstXI CCAn_nnnn'nTGG Cuts at: 2846 3406 3427 4120 4405 4592 4894 5626 5797 Size: 560 21 693 285 187 302 732 171 Cuts at: 5797 5933 2846 Size: 136 5647 Fragments arranged by size: 5647 732 693 560 302 285 187 171 136 21 BstYI r'GATC_y Cuts at: 1972 2127 3267 5664 6064 6431 6816 6833 8285 Size: 155 1140 2397 400 367 385 17 1452 Cuts at: 8285 8371 8383 8729 1972 Size: 86 12 346 1977 Fragments arranged by size: 2397 1977 1452 1140 400 385 367 346 155 86 17 12 Bsu36I CC'TnA_GG Cuts at: 5131 5131 Size: 8734 BtgI C'CryG_G Cuts at: 513 1065 2043 4287 4471 4791 5385 5500 6021 Size: 552 978 2244 184 320 594 115 521 Cuts at: 6021 513 Size: 3226 Fragments arranged by size: 3226 2244 978 594 552 521 320 184 115 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2044 3343 3360 4645 4693 487 Size: 683 56 818 1299 17 1285 48 4528 Fragments arranged by size: 4528 1299 1285 818 683 56 48 17 BtsI GCAGTG_nn' Cuts at: 3829 3880 4614 5711 6250 7044 7064 3829 Size: 51 734 1097 539 794 20 5499 Fragments arranged by size: 5499 1097 794 734 539 51 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8734 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2025 2060 4824 4859 5155 5190 529 Size: 35 1461 35 2764 35 296 35 4073 Fragments arranged by size: 4073 2764 1461 296 35 35 35 35 DraI TTT'AAA Cuts at: 6038 6038 Size: 8734 DraIII CAC_nnn'GTG Cuts at: 2188 3099 2188 Size: 911 7823 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7741 809 Size: 507 6425 1802 Fragments arranged by size: 6425 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2141 3555 4250 Size: 54 1425 50 275 18 311 1414 695 Cuts at: 4250 4992 5787 5830 5970 5998 6181 7070 8699 Size: 742 795 43 140 28 183 889 1629 Cuts at: 8699 8 Size: 43 Fragments arranged by size: 1629 1425 1414 889 795 742 695 311 275 183 140 54 50 43 43 28 18 EagI C'GGCC_G Cuts at: 1537 1812 2141 4250 5970 6181 1537 Size: 275 329 2109 1720 211 4090 Fragments arranged by size: 4090 2109 1720 329 275 211 EarI CTCTTCn'nnn_ Cuts at: 1353 2081 2466 4175 4181 4283 4817 6664 1353 Size: 728 385 1709 6 102 534 1847 3423 Fragments arranged by size: 3423 1847 1709 728 534 385 102 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3413 4628 5945 7321 7695 7841 Size: 1064 138 1824 1215 1317 1376 374 146 Cuts at: 7841 387 Size: 1280 Fragments arranged by size: 1824 1376 1317 1280 1215 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2812 3024 3105 2812 Size: 212 81 8441 Fragments arranged by size: 8441 212 81 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2153 2382 2759 3184 3886 3929 4331 Size: 393 521 229 377 425 702 43 402 Cuts at: 4331 4463 4547 4870 5299 5585 5771 6026 6797 Size: 132 84 323 429 286 186 255 771 Cuts at: 6797 7394 8181 8449 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 787 771 702 597 521 429 425 402 393 377 323 286 268 255 229 186 132 84 43 EcoNI CCTnn'n_nnAGG Cuts at: 2237 2237 Size: 8734 EcoO109I rG'GnC_Cy Cuts at: 2476 2513 2514 3055 3325 3970 5067 5410 6026 Size: 37 1 541 270 645 1097 343 616 Cuts at: 6026 2476 Size: 5184 Fragments arranged by size: 5184 1097 645 616 541 343 270 37 1 EcoRI G'AATT_C Cuts at: 6043 6421 6043 Size: 378 8356 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2572 5575 5833 2572 Size: 3003 258 5473 Fragments arranged by size: 5473 3003 258 FseI GG_CCGG'CC Cuts at: 4256 5446 5683 4256 Size: 1190 237 7307 Fragments arranged by size: 7307 1190 237 FspI TGC'GCA Cuts at: 2572 3048 5807 7240 2572 Size: 476 2759 1433 4066 Fragments arranged by size: 4066 2759 1433 476 FspAI rTGC'GCAy Cuts at: 2572 3048 2572 Size: 476 8258 HaeII r_GCGC'y Cuts at: 1678 3321 3405 4333 4599 5643 5804 7881 1678 Size: 1643 84 928 266 1044 161 2077 2531 Fragments arranged by size: 2531 2077 1643 1044 928 266 161 84 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2828 2860 3008 3040 3044 Size: 32 135 32 1256 32 148 32 4 Cuts at: 3044 3076 3288 3317 3320 3349 3785 3817 4379 Size: 32 212 29 3 29 436 32 562 Cuts at: 4379 4411 4832 4864 7375 7407 7449 7481 1373 Size: 32 421 32 2511 32 42 32 2626 Fragments arranged by size: 2626 2511 1256 562 436 421 212 148 135 42 32 32 32 32 32 32 32 32 32 32 29 29 4 3 HincII GTy'rAC Cuts at: 669 1657 1938 2496 3943 4836 6078 6324 669 Size: 988 281 558 1447 893 1242 246 3079 Fragments arranged by size: 3079 1447 1242 988 893 558 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8734 HpaI GTT'AAC Cuts at: 6324 6324 Size: 8734 KasI G'GCGC_C Cuts at: 3317 4595 3317 Size: 1278 7456 KpnI G_GTAC'C Cuts at: 6059 6059 Size: 8734 MfeI C'AATT_G Cuts at: 6333 6333 Size: 8734 MluI A'CGCG_T Cuts at: 8610 8610 Size: 8734 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3087 3498 4533 7848 8032 1305 Size: 1782 411 1035 3315 184 2007 Fragments arranged by size: 3315 2007 1782 1035 411 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3557 10 Size: 54 1425 343 1725 5187 Fragments arranged by size: 5187 1725 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3380 3386 3815 5282 5432 5795 5963 Size: 648 2214 6 429 1467 150 363 168 Cuts at: 5963 6692 7051 7210 518 Size: 729 359 159 2042 Fragments arranged by size: 2214 2042 1467 729 648 429 363 359 168 159 150 6 MspA1I CmG'CkG Cuts at: 1606 2068 2118 2217 2410 2509 3214 3247 4189 Size: 462 50 99 193 99 705 33 942 Cuts at: 4189 4246 4289 4318 4381 5464 5542 5749 5892 Size: 57 43 29 63 1083 78 207 143 Cuts at: 5892 5944 6023 6150 6827 7975 8220 1606 Size: 52 79 127 677 1148 245 2120 Fragments arranged by size: 2120 1148 1083 942 705 677 462 245 207 193 143 127 99 99 79 78 63 57 52 50 43 33 29 NaeI GCC'GGC Cuts at: 4254 5444 5681 5974 4254 Size: 1190 237 293 7014 Fragments arranged by size: 7014 1190 293 237 NarI GG'CG_CC Cuts at: 3318 4596 3318 Size: 1278 7456 NcoI C'CATG_G Cuts at: 513 1065 5500 513 Size: 552 4435 3747 Fragments arranged by size: 4435 3747 552 NdeI CA'TA_TG Cuts at: 387 2104 387 Size: 1717 7017 NgoMIV G'CCGG_C Cuts at: 4252 5442 5679 5972 4252 Size: 1190 237 293 7014 Fragments arranged by size: 7014 1190 293 237 NheI G'CTAG_C Cuts at: 1052 5339 1052 Size: 4287 4447 NotI GC'GGCC_GC Cuts at: 6181 6181 Size: 8734 NspI r_CATG'y Cuts at: 1132 1229 2206 2614 3784 5455 6092 7637 1132 Size: 97 977 408 1170 1671 637 1545 2229 Fragments arranged by size: 2229 1671 1545 1170 977 637 408 97 PasI CC'CwG_GG Cuts at: 1348 3406 4138 1348 Size: 2058 732 5944 Fragments arranged by size: 5944 2058 732 PciI A'CATG_T Cuts at: 7633 7633 Size: 8734 PflMI CCAn_nnn'nTGG Cuts at: 1108 3247 4827 5506 1108 Size: 2139 1580 679 4336 Fragments arranged by size: 4336 2139 1580 679 PfoI T'CCnGG_A Cuts at: 2130 2730 3306 4440 4485 5852 2130 Size: 600 576 1134 45 1367 5012 Fragments arranged by size: 5012 1367 1134 600 576 45 PmeI GTTT'AAAC Cuts at: 6038 6038 Size: 8734 PmlI CAC'GTG Cuts at: 3274 4972 3274 Size: 1698 7036 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3062 3094 6803 6835 8316 8348 3062 Size: 32 3709 32 1481 32 3448 Fragments arranged by size: 3709 3448 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2476 3055 3325 3970 5410 2476 Size: 579 270 645 1440 5800 Fragments arranged by size: 5800 1440 645 579 270 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8734 PsiI TTA'TAA Cuts at: 2012 2349 6304 2012 Size: 337 3955 4442 Fragments arranged by size: 4442 3955 337 PspOMI G'GGCC_C Cuts at: 2513 5066 5894 6025 2513 Size: 2553 828 131 5222 Fragments arranged by size: 5222 2553 828 131 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2129 2161 2129 Size: 32 8702 PstI C_TGCA'G Cuts at: 830 5700 6086 830 Size: 4870 386 3478 Fragments arranged by size: 4870 3478 386 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8734 PvuII CAG'CTG Cuts at: 2068 2217 2410 3214 4246 5749 2068 Size: 149 193 804 1032 1503 5053 Fragments arranged by size: 5053 1503 1032 804 193 149 SacI G_AGCT'C Cuts at: 2814 3026 3107 2814 Size: 212 81 8441 Fragments arranged by size: 8441 212 81 SacII CC_GC'GG Cuts at: 4290 6024 4290 Size: 1734 7000 SalI G'TCGA_C Cuts at: 1936 3941 6076 1936 Size: 2005 2135 4594 Fragments arranged by size: 4594 2135 2005 SanDI GG'GwC_CC Cuts at: 3325 3325 Size: 8734 SapI GCTCTTCn'nnn_ Cuts at: 2081 2081 Size: 8734 SbfI CC_TGCA'GG Cuts at: 6086 6086 Size: 8734 ScaI AGT'ACT Cuts at: 6982 8694 6982 Size: 1712 7022 SexAI A'CCwGG_T Cuts at: 2294 2294 Size: 8734 SfcI C'TryA_G Cuts at: 826 1045 3487 3721 5696 6082 7217 7898 8089 Size: 219 2442 234 1975 386 1135 681 191 Cuts at: 8089 826 Size: 1471 Fragments arranged by size: 2442 1975 1471 1135 681 386 234 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 5829 5829 Size: 8734 SfoI GGC'GCC Cuts at: 3319 4597 3319 Size: 1278 7456 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8734 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8734 SmaI CCC'GGG Cuts at: 1872 3807 4539 6061 1872 Size: 1935 732 1522 4545 Fragments arranged by size: 4545 1935 1522 732 SmlI C'TyrA_G Cuts at: 820 1017 1943 2903 4207 6838 7739 8001 820 Size: 197 926 960 1304 2631 901 262 1553 Fragments arranged by size: 2631 1553 1304 960 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 6525 493 Size: 6032 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8734 SphI G_CATG'C Cuts at: 1132 2206 2614 3784 6092 1132 Size: 1074 408 1170 2308 3774 Fragments arranged by size: 3774 2308 1170 1074 408 SrfI GCCC'GGGC Cuts at: 4539 4539 Size: 8734 SspI AAT'ATT Cuts at: 5 52 6658 5 Size: 47 6606 2081 Fragments arranged by size: 6606 2081 47 StuI AGG'CCT Cuts at: 1517 2684 4046 1517 Size: 1167 1362 6205 Fragments arranged by size: 6205 1362 1167 StyI C'CwwG_G Cuts at: 513 1065 2238 3084 4940 5292 5367 5500 5619 Size: 552 1173 846 1856 352 75 133 119 Cuts at: 5619 6030 6173 513 Size: 411 143 3074 Fragments arranged by size: 3074 1856 1173 846 552 411 352 143 133 119 75 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2928 5210 6761 6778 6931 7116 7561 Size: 1255 1348 2282 1551 17 153 185 445 Cuts at: 7561 325 Size: 1498 Fragments arranged by size: 2282 1551 1498 1348 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2070 2175 2541 Size: 274 80 33 51 1443 93 105 366 Cuts at: 2541 4761 5634 5754 6980 8692 96 Size: 2220 873 120 1226 1712 138 Fragments arranged by size: 2220 1712 1443 1226 873 366 274 138 120 105 93 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 8694 8726 8694 Size: 32 8702 Tth111I GACn'n_nGTC Cuts at: 1316 2432 3377 3686 4844 5078 1316 Size: 1116 945 309 1158 234 4972 Fragments arranged by size: 4972 1158 1116 945 309 234 XbaI T'CTAG_A Cuts at: 6070 6070 Size: 8734 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3115 4018 4947 5623 3115 Size: 903 929 676 6226 Fragments arranged by size: 6226 929 903 676 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8734 XmaI C'CCGG_G Cuts at: 1870 3805 4537 6059 1870 Size: 1935 732 1522 4545 Fragments arranged by size: 4545 1935 1522 732 XmnI GAAnn'nnTTC Cuts at: 6863 6863 Size: 8734 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6542 276 Size: 53 83 186 936 5008 2468 Fragments arranged by size: 5008 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BfrBI BsiWI BstZ17I EcoRV NruI NsiI PacI PspXI RsrII SwaI