(Circular) (Six-base) MAPSORT of: pFN21AA1450.seq Check: 4517 from: 1 to: 8047 pFN21AA1450 8047 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5857 278 Size: 53 83 186 936 4321 2468 Fragments arranged by size: 4321 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5390 1937 Size: 3453 4594 Acc65I G'GTAC_C Cuts at: 5368 5368 Size: 8047 AclI AA'CG_TT Cuts at: 1563 6174 6547 1563 Size: 4611 373 3063 Fragments arranged by size: 4611 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2910 3065 4630 4674 4707 4934 5001 5093 Size: 1278 155 1565 44 33 227 67 92 Cuts at: 5093 6110 7494 7762 1632 Size: 1017 1384 268 1917 Fragments arranged by size: 1917 1565 1384 1278 1017 268 227 155 92 67 44 33 AfeI AGC'GCT Cuts at: 3251 3251 Size: 8047 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7850 AflIII A'CryG_T Cuts at: 1203 2412 6946 7923 1203 Size: 1209 4534 977 1327 Fragments arranged by size: 4534 1327 1209 977 AgeI A'CCGG_T Cuts at: 4092 6878 4092 Size: 2786 5261 AhdI GACnn_n'nnGTC Cuts at: 6776 6776 Size: 8047 AleI CACnn'nnGTG Cuts at: 1166 3518 4749 1166 Size: 2352 1231 4464 Fragments arranged by size: 4464 2352 1231 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8047 AlwNI CAG_nnn'CTG Cuts at: 2541 4190 4924 5790 7362 2541 Size: 1649 734 866 1572 3226 Fragments arranged by size: 3226 1649 1572 866 734 ApaI G_GGCC'C Cuts at: 4589 4589 Size: 8047 ApaLI G'TGCA_C Cuts at: 6104 7260 6104 Size: 1156 6891 ApoI r'AATT_y Cuts at: 1493 2986 3146 5356 5582 5734 7843 1493 Size: 1493 160 2210 226 152 2109 1697 Fragments arranged by size: 2210 2109 1697 1493 226 160 152 AseI AT'TA_AT Cuts at: 160 1032 6601 160 Size: 872 5569 1606 Fragments arranged by size: 5569 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8047 AvaI C'yCGr_G Cuts at: 1870 1943 5372 1870 Size: 73 3429 4545 Fragments arranged by size: 4545 3429 73 BamHI G'GATC_C Cuts at: 5377 5744 5377 Size: 367 7680 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3900 3973 5368 6823 618 Size: 325 217 627 2113 73 1395 1455 1842 Fragments arranged by size: 2113 1842 1455 1395 627 325 217 73 BanII G_rGCy'C Cuts at: 1391 2089 3732 4304 4589 5366 1391 Size: 698 1643 572 285 777 4072 Fragments arranged by size: 4072 1643 777 698 572 285 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2509 2709 928 Size: 971 610 200 6266 Fragments arranged by size: 6266 971 610 200 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6238 6272 1903 Size: 34 4301 34 3678 Fragments arranged by size: 4301 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5939 7149 5939 Size: 1210 6837 BclI T'GATC_A Cuts at: 1548 2751 1548 Size: 1203 6844 BfrBI ATG'CAT Cuts at: 3944 5164 3944 Size: 1220 6827 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3813 4262 6658 Size: 107 122 71 631 605 2141 449 2396 Cuts at: 6658 136 Size: 1525 Fragments arranged by size: 2396 2141 1525 631 605 449 122 107 71 BglII A'GATC_T Cuts at: 4735 8042 4735 Size: 3307 4740 BlpI GC'TnA_GC Cuts at: 4442 5464 4442 Size: 1022 7025 Bme1580I G_kGCm'C Cuts at: 1790 3463 3809 3818 4466 4589 6108 7264 1790 Size: 1673 346 9 648 123 1519 1156 2573 Fragments arranged by size: 2573 1673 1519 1156 648 346 123 9 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8047 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3406 5276 5778 Size: 416 53 83 399 24 1989 1870 502 Cuts at: 5778 6736 442 Size: 958 1753 Fragments arranged by size: 1989 1870 1753 958 502 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8047 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3525 3525 Size: 8047 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2006 3480 3634 4167 4526 4574 4782 6707 Size: 767 1474 154 533 359 48 208 1925 Cuts at: 6707 1239 Size: 2579 Fragments arranged by size: 2579 1925 1474 767 533 359 208 154 48 Bpu10I CC'TnA_GC Cuts at: 2361 4278 2361 Size: 1917 6130 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3242 6172 7037 7335 3242 Size: 2930 865 298 3954 Fragments arranged by size: 3954 2930 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3822 4803 5089 6710 882 Size: 617 2323 981 286 1621 2219 Fragments arranged by size: 2323 2219 1621 981 617 286 BsaAI yAC'GTr Cuts at: 493 1204 5838 493 Size: 711 4634 2702 Fragments arranged by size: 4634 2702 711 BsaBI GATnn'nnATC Cuts at: 8041 8041 Size: 8047 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2069 5854 6236 6871 Size: 53 83 186 936 536 3785 382 635 Cuts at: 6871 275 Size: 1451 Fragments arranged by size: 3785 1451 936 635 536 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4092 6480 6878 7152 7299 7710 4092 Size: 2388 398 274 147 411 4429 Fragments arranged by size: 4429 2388 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2444 2474 3686 3716 5305 Size: 30 165 30 1041 30 1212 30 1589 Cuts at: 5305 5335 1178 Size: 30 3890 Fragments arranged by size: 3890 1589 1212 1041 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2237 2481 2767 3707 4513 1186 Size: 1051 244 286 940 806 4720 Fragments arranged by size: 4720 1051 940 806 286 244 BseYI C'CCAG_C Cuts at: 3466 4001 4433 7250 7746 3466 Size: 535 432 2817 496 3767 Fragments arranged by size: 3767 2817 535 496 432 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4500 5042 1163 Size: 573 2764 542 4168 Fragments arranged by size: 4168 2764 573 542 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5497 6258 7286 1540 Size: 275 186 3496 761 1028 2301 Fragments arranged by size: 3496 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 6108 6193 7264 6108 Size: 85 1071 6891 Fragments arranged by size: 6891 1071 85 BsmI GAATG_Cn' Cuts at: 3946 5558 5651 3946 Size: 1612 93 6342 Fragments arranged by size: 6342 1612 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2089 3463 3732 3809 3818 4304 4466 Size: 399 299 1374 269 77 9 486 162 Cuts at: 4466 4589 5366 6108 6193 7264 1391 Size: 123 777 742 85 1071 2174 Fragments arranged by size: 2174 1374 1071 777 742 486 399 299 269 162 123 85 77 9 BspEI T'CCGG_A Cuts at: 7710 7710 Size: 8047 BspHI T'CATG_A Cuts at: 5936 7666 5936 Size: 1730 6317 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4279 5014 5295 5402 844 Size: 3435 735 281 107 3489 Fragments arranged by size: 3489 3435 735 281 107 BsrBI CCG'CTC Cuts at: 1394 1823 3734 4839 5934 1394 Size: 429 1911 1105 1095 3507 Fragments arranged by size: 3507 1911 1105 1095 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2718 6542 6716 65 Size: 1183 1470 3824 174 1396 Fragments arranged by size: 3824 1470 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4092 4895 6691 6878 1700 Size: 110 2282 803 1796 187 2869 Fragments arranged by size: 2869 2282 1796 803 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8047 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8047 BssSI C'ACGA_G Cuts at: 4595 5235 6107 7119 4595 Size: 640 872 1012 5523 Fragments arranged by size: 5523 1012 872 640 BstAPI GCAn_nnn'nTGC Cuts at: 794 4430 794 Size: 3636 4411 BstBI TT'CG_AA Cuts at: 2818 6851 2818 Size: 4033 4014 BstEII G'GTnAC_C Cuts at: 1184 3635 1184 Size: 2451 5596 BstYI r'GATC_y Cuts at: 1972 2345 2590 4735 5377 5744 6129 6146 7598 Size: 373 245 2145 642 367 385 17 1452 Cuts at: 7598 7684 7696 8042 1972 Size: 86 12 346 1977 Fragments arranged by size: 2145 1977 1452 642 385 373 367 346 245 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2091 2091 Size: 8047 BtgI C'CryG_G Cuts at: 513 1065 2004 2061 4474 513 Size: 552 939 57 2413 4086 Fragments arranged by size: 4086 2413 939 552 57 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7308 Fragments arranged by size: 7308 683 56 BtsI GCAGTG_nn' Cuts at: 2299 4906 4925 5563 6357 6377 2299 Size: 2607 19 638 794 20 3969 Fragments arranged by size: 3969 2607 794 638 20 19 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8047 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8012 DraI TTT'AAA Cuts at: 3648 4309 5351 3648 Size: 661 1042 6344 Fragments arranged by size: 6344 1042 661 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4765 7054 809 Size: 507 3449 2289 1802 Fragments arranged by size: 3449 2289 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3429 3785 4834 Size: 54 1425 50 275 18 1599 356 1049 Cuts at: 4834 4929 5494 6383 8012 8 Size: 95 565 889 1629 43 Fragments arranged by size: 1629 1599 1425 1049 889 565 356 275 95 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5494 1537 Size: 275 3682 4090 Fragments arranged by size: 4090 3682 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2036 2329 3041 3994 5977 1353 Size: 683 293 712 953 1983 3423 Fragments arranged by size: 3423 1983 953 712 683 293 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6634 7008 7154 387 Size: 1064 138 5045 374 146 1280 Fragments arranged by size: 5045 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2006 2910 3065 3480 3634 4167 4526 Size: 393 374 904 155 415 154 533 359 Cuts at: 4526 4574 4630 4674 4707 4782 4934 5001 5093 Size: 48 56 44 33 75 152 67 92 Cuts at: 5093 6110 6707 7494 7762 1239 Size: 1017 597 787 268 1524 Fragments arranged by size: 1524 1017 904 787 597 533 415 393 374 359 268 155 154 152 92 75 67 56 48 44 33 EcoNI CCTnn'n_nnAGG Cuts at: 2094 2094 Size: 8047 EcoO109I rG'GnC_Cy Cuts at: 2099 2633 3349 4282 4544 4585 4586 4767 2099 Size: 534 716 933 262 41 1 181 5379 Fragments arranged by size: 5379 933 716 534 262 181 41 1 EcoRI G'AATT_C Cuts at: 3146 5356 5734 3146 Size: 2210 378 5459 Fragments arranged by size: 5459 2210 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2415 4660 2415 Size: 2245 5802 FspI TGC'GCA Cuts at: 6553 6553 Size: 8047 HaeII r_GCGC'y Cuts at: 1678 2809 3253 4692 7194 1678 Size: 1131 444 1439 2502 2531 Fragments arranged by size: 2531 2502 1439 1131 444 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3121 3144 3153 3176 3607 Size: 32 135 32 1549 23 9 23 431 Cuts at: 3607 3639 6688 6720 6762 6794 1373 Size: 32 3049 32 42 32 2626 Fragments arranged by size: 3049 2626 1549 431 135 42 32 32 32 32 32 23 23 9 HincII GTy'rAC Cuts at: 669 1657 1938 2410 2865 3330 5298 5391 5637 Size: 988 281 472 455 465 1968 93 246 Cuts at: 5637 669 Size: 3079 Fragments arranged by size: 3079 1968 988 472 465 455 281 246 93 HindIII A'AGCT_T Cuts at: 748 4171 5132 748 Size: 3423 961 3663 Fragments arranged by size: 3663 3423 961 HpaI GTT'AAC Cuts at: 3330 5637 3330 Size: 2307 5740 KpnI G_GTAC'C Cuts at: 5372 5372 Size: 8047 MfeI C'AATT_G Cuts at: 3054 5646 3054 Size: 2592 5455 MluI A'CGCG_T Cuts at: 7923 7923 Size: 8047 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5304 7161 7345 1305 Size: 3999 1857 184 2007 Fragments arranged by size: 3999 2007 1857 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3431 10 Size: 54 1425 343 1599 4626 Fragments arranged by size: 4626 1599 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3365 3518 3716 4749 4868 6005 6364 Size: 648 2199 153 198 1033 119 1137 359 Cuts at: 6364 6523 518 Size: 159 2042 Fragments arranged by size: 2199 2042 1137 1033 648 359 198 159 153 119 MspA1I CmG'CkG Cuts at: 1606 2052 2063 2811 3466 3607 4005 4582 5463 Size: 446 11 748 655 141 398 577 881 Cuts at: 5463 6140 7288 7533 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 881 748 677 655 577 446 398 245 141 11 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 6556 Fragments arranged by size: 6556 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8047 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8047 NotI GC'GGCC_GC Cuts at: 5494 5494 Size: 8047 NsiI A_TGCA'T Cuts at: 3946 5166 3946 Size: 1220 6827 NspI r_CATG'y Cuts at: 1132 1229 2416 3427 4873 5405 6950 1132 Size: 97 1187 1011 1446 532 1545 2229 Fragments arranged by size: 2229 1545 1446 1187 1011 532 97 PasI CC'CwG_GG Cuts at: 1348 2077 4693 1348 Size: 729 2616 4702 Fragments arranged by size: 4702 2616 729 PciI A'CATG_T Cuts at: 2412 6946 2412 Size: 4534 3513 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 8047 PfoI T'CCnGG_A Cuts at: 2991 3926 4128 4962 2991 Size: 935 202 834 6076 Fragments arranged by size: 6076 935 834 202 PmeI GTTT'AAAC Cuts at: 5351 5351 Size: 8047 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6116 6148 7629 7661 6116 Size: 32 1481 32 6502 Fragments arranged by size: 6502 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2099 2633 3349 4282 4544 4767 2099 Size: 534 716 933 262 223 5379 Fragments arranged by size: 5379 933 716 534 262 223 PshAI GACnn'nnGTC Cuts at: 1613 3834 1613 Size: 2221 5826 PsiI TTA'TAA Cuts at: 5617 5617 Size: 8047 PspOMI G'GGCC_C Cuts at: 4585 4585 Size: 8047 PstI C_TGCA'G Cuts at: 830 5399 830 Size: 4569 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8047 PvuII CAG'CTG Cuts at: 3466 3607 4005 3466 Size: 141 398 7508 Fragments arranged by size: 7508 398 141 SacII CC_GC'GG Cuts at: 2064 2064 Size: 8047 SalI G'TCGA_C Cuts at: 1936 5389 1936 Size: 3453 4594 SanDI GG'GwC_CC Cuts at: 3349 3349 Size: 8047 SapI GCTCTTCn'nnn_ Cuts at: 2036 3994 2036 Size: 1958 6089 SbfI CC_TGCA'GG Cuts at: 5399 5399 Size: 8047 ScaI AGT'ACT Cuts at: 5314 6295 8007 5314 Size: 981 1712 5354 Fragments arranged by size: 5354 1712 981 SfcI C'TryA_G Cuts at: 826 1045 2899 3076 5180 5395 6530 7211 7402 Size: 219 1854 177 2104 215 1135 681 191 Cuts at: 7402 826 Size: 1471 Fragments arranged by size: 2104 1854 1471 1135 681 219 215 191 177 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8047 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8047 SmaI CCC'GGG Cuts at: 1872 5374 1872 Size: 3502 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3257 6151 7052 7314 820 Size: 197 926 1314 2894 901 262 1553 Fragments arranged by size: 2894 1553 1314 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5838 493 Size: 5345 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8047 SphI G_CATG'C Cuts at: 1132 5405 1132 Size: 4273 3774 SspI AAT'ATT Cuts at: 5 52 5971 5 Size: 47 5919 2081 Fragments arranged by size: 5919 2081 47 StuI AGG'CCT Cuts at: 1517 4235 1517 Size: 2718 5329 StyI C'CwwG_G Cuts at: 513 1065 2004 3182 3672 4248 4770 4793 5486 Size: 552 939 1178 490 576 522 23 693 Cuts at: 5486 513 Size: 3074 Fragments arranged by size: 3074 1178 939 693 576 552 522 490 23 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3458 4829 6074 6091 6244 6429 6874 Size: 1255 1878 1371 1245 17 153 185 445 Cuts at: 6874 325 Size: 1498 Fragments arranged by size: 1878 1498 1371 1255 1245 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2385 2549 2577 Size: 274 80 33 51 1443 408 164 28 Cuts at: 2577 5312 6293 8005 96 Size: 2735 981 1712 138 Fragments arranged by size: 2735 1712 1443 981 408 274 164 138 80 51 33 28 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4885 4917 8007 8039 4885 Size: 32 3090 32 4893 Fragments arranged by size: 4893 3090 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3542 4937 1316 Size: 2226 1395 4426 Fragments arranged by size: 4426 2226 1395 XbaI T'CTAG_A Cuts at: 5383 5383 Size: 8047 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2336 3647 4586 4735 2336 Size: 1311 939 149 5648 Fragments arranged by size: 5648 1311 939 149 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8047 XmaI C'CCGG_G Cuts at: 1870 5372 1870 Size: 3502 4545 XmnI GAAnn'nnTTC Cuts at: 2030 2370 6176 2030 Size: 340 3806 3901 Fragments arranged by size: 3901 3806 340 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5855 276 Size: 53 83 186 936 4321 2468 Fragments arranged by size: 4321 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BbvCI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspXI PsrI RsrII SacI SexAI SfiI SfoI SrfI SwaI