Restriction map for
pFN21AA1449
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# NO CUT ENZYMES ( 122 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI Asp718I AspEI AspHI AvaI
AviII AvrII BanI BanII BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfrI BlnI BlpI Bpu1102I
BpuAI BsaBI BsaHI BsaWI BseAI
BsiHKAI BsiWI BsmBI BsoBI BspDI
BspEI BspHI BssHII BssSI Bst1107I
BstEII BstPI BstUI BstZ17I Bsu36I
CelII ClaI CpoI DraII DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRV FauI FbaI FseI
FspI HgaI Hin1I KasI KpnI
KspI MamI MfeI MluI MroI
MunI MvnI NaeI NarI NcoI
NgoMIV NheI NotI NruI PacI
PaeR7I PinAI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SbfI
ScaI SfiI SfoI SgrAI SmaI
SphI Sse8387I SwaI XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AseI 267 ATTAAT AT^TAAT
AsnI 267 ATTAAT AT^TAAT
Asp700I 1971 GAAACACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1608 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcnI 1598 CCGGG CC^[GC]GG
BglI 674 GCCTGTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1188 AGATCT A^GATCT
BsaAI 355 TACGTA [CT]AC^GT[GA]
BsaI 1997 GAGACC GGTCTC(1/5)
BsiEI 144 CGGTCG CG[GA][CT]^CG
BsmI 306 GAATGC GAATGC(1/-1)
BspMI 45 GCAGGT ACCTGC(4/8)
BsrBI 1989 GAGCGG CCGCTC(-3/-3)
BstBI 1961 TTCGAA TT^CGAA
BstXI 1470 CCATGTAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 769 GCGC GCG^C
DraI 1231 TTTAAA TTT^AAA
EcoRI 983 GAATTC G^AATTC
EcoT22I 1715 ATGCAT ATGCA^T
HaeII 768 GGCGCT [GA]GCGC^[CT]
HhaI 769 GCGC GCG^C
HinP1I 769 GCGC G^CGC
HindIII 613 AAGCTT A^AGCTT
HpaI 1284 GTTAAC GTT^AAC
MaeII 356 ACGT A^CGT
NciI 1598 CCGGG CC^[GC]GG
NsiI 1715 ATGCAT ATGCA^T
NspV 1961 TTCGAA TT^CGAA
PflFI 1608 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1935 CCAGGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 267 ATTAAT AT^TAAT
PstI 106 CTGCAG CTGCA^G
SfuI 1961 TTCGAA TT^CGAA
SmlI 892 CTCAAG C^T[CT][GA]AG
SnaBI 355 TACGTA TAC^GTA
SpeI 390 ACTAGT A^CTAGT
StuI 362 AGGCCT AGG^CCT
Tth111I 1608 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1935 CCAGGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1971 GAAACACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 954 GTCTAC GT^[AC][GT]AC
1264 GTAGAC
BalI 521 TGGCCA TGG^CCA
717 TGGCCA
BmyI 622 GGGCAC G[AGT]GC[ACT]^C
1525 GTGCCC
BpmI 1004 CTGGAG CTGGAG(16/14)
1559 CTGGAG
BseRI 923 CTCCTC GAGGAG(10/8)
1906 GAGGAG
BsgI 43 GTGCAG GTGCAG(16/14)
1498 GTGCAG
Bsp1286I 622 GGGCAC G[AGT]GC[ACT]^C
1525 GTGCCC
BsrDI 644 CATTGC GCAATG(2/0)
669 GCAATG
BsrFI 14 ACCGGC [GA]^CCGG[CT]
142 GCCGGT
BsrI 534 ACTGG ACTGG(1/-1)
886 CCAGT
Cac8I 665 GCACGC GC[ACGT]^[ACGT]GC
719 GCCAGC
Cfr10I 14 ACCGGC [GA]^CCGG[CT]
142 GCCGGT
EaeI 521 TGGCCA [CT]^GGCC[GA]
717 TGGCCA
EcoT14I 377 CCAAGG C^C[AT][AT]GG
714 CCTTGG
HincII 330 GTCAAC GT[CT]^[GA]AC
1284 GTTAAC
HindII 330 GTCAAC GT[CT]^[GA]AC
1284 GTTAAC
MluNI 521 TGGCCA TGG^CCA
717 TGGCCA
MscI 521 TGGCCA TGG^CCA
717 TGGCCA
NdeI 371 CATATG CA^TATG
1980 CATATG
SapI 1986 GAAGAGC GCTCTTC(1/4)
1844 GCTCTTC
SexAI 1061 ACCAGGT A^CC[AT]GGT
1934 ACCAGGT
SfcI 106 CTGCAG C^T[GA][CT]AG
956 CTACAG
SspI 420 AATATT AAT^ATT
1415 AATATT
StyI 377 CCAAGG C^C[AT][AT]GG
714 CCTTGG
Tsp45I 475 GTCAC ^GT[GC]AC
1763 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AvaII BamHI BbvI
BfaI BsaJI BsiYI BslI BsmAI
BsmFI Bsp1407I BspLU11I BsrGI BssKI
BstAPI BstF5I BstNI BstYI Cfr13I
DdeI DpnII EarI EcoRII Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SspBI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I XcmI
XhoII