Restriction map for
pFN21AA1449
(Circular) (Six-base) MAPSORT of: pFN21AA1449.seq Check: 2854 from: 1 to: 6736
pFN21AA1449 6736 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4546 278
Size: 53 83 186 936 3010 2468
Fragments arranged by size:
3010 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2960 3270 4079 1937
Size: 1023 310 809 4594
Fragments arranged by size:
4594 1023 809 310
Acc65I G'GTAC_C
Cuts at: 4057 4057
Size: 6736
AclI AA'CG_TT
Cuts at: 1563 4863 5236 1563
Size: 3300 373 3063
Fragments arranged by size:
3300 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3711 4799 6183 6451 1632
Size: 2079 1088 1384 268 1917
Fragments arranged by size:
2079 1917 1384 1088 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6539
AflIII A'CryG_T
Cuts at: 1203 2600 2800 3436 3799 5635 6612 1203
Size: 1397 200 636 363 1836 977 1327
Fragments arranged by size:
1836 1397 1327 977 636 363 200
AgeI A'CCGG_T
Cuts at: 5567 5567
Size: 6736
AhdI GACnn_n'nnGTC
Cuts at: 5465 5465
Size: 6736
AleI CACnn'nnGTG
Cuts at: 1166 2801 1166
Size: 1635 5101
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6736
AlwNI CAG_nnn'CTG
Cuts at: 4479 6051 4479
Size: 1572 5164
ApaLI G'TGCA_C
Cuts at: 4793 5949 4793
Size: 1156 5580
ApoI r'AATT_y
Cuts at: 1493 2988 3385 3686 3696 4045 4271 4423 6532
Size: 1495 397 301 10 349 226 152 2109
Cuts at: 6532 1493
Size: 1697
Fragments arranged by size:
2109 1697 1495 397 349 301 226 152
10
AseI AT'TA_AT
Cuts at: 160 1032 2273 5290 160
Size: 872 1241 3017 1606
Fragments arranged by size:
3017 1606 1241 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6736
AvaI C'yCGr_G
Cuts at: 1870 1943 4061 1870
Size: 73 2118 4545
Fragments arranged by size:
4545 2118 73
BamHI G'GATC_C
Cuts at: 2119 2590 3949 4066 4433 2119
Size: 471 1359 117 367 4422
Fragments arranged by size:
4422 1359 471 367 117
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4057 5512 618
Size: 325 217 627 2270 1455 1842
Fragments arranged by size:
2270 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4055 1391
Size: 2664 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5765
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4927 4961 1903
Size: 34 2990 34 3678
Fragments arranged by size:
3678 2990 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4628 5838 4628
Size: 1210 5526
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6736
BfrBI ATG'CAT
Cuts at: 3722 3722
Size: 6736
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2685 5347
Size: 107 122 71 631 605 337 676 2662
Cuts at: 5347 136
Size: 1525
Fragments arranged by size:
2662 1525 676 631 605 337 122 107
71
BglII A'GATC_T
Cuts at: 3193 6731 3193
Size: 3538 3198
BlpI GC'TnA_GC
Cuts at: 4153 4153
Size: 6736
Bme1580I G_kGCm'C
Cuts at: 1790 2631 3534 4797 5953 1790
Size: 841 903 1263 1156 2573
Fragments arranged by size:
2573 1263 1156 903 841
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6736
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2549 4467 5425
Size: 416 53 83 399 24 1132 1918 958
Cuts at: 5425 442
Size: 1753
Fragments arranged by size:
1918 1753 1132 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6736
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3030 3585 5396 1239
Size: 1791 555 1811 2579
Fragments arranged by size:
2579 1811 1791 555
Bpu10I CC'TnA_GC
Cuts at: 2371 2371
Size: 6736
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2882 4861 5726 6024 2882
Size: 1979 865 298 3594
Fragments arranged by size:
3594 1979 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3996 5399 882
Size: 617 2497 1403 2219
Fragments arranged by size:
2497 2219 1403 617
BsaAI yAC'GTr
Cuts at: 493 1204 2362 4527 493
Size: 711 1158 2165 2702
Fragments arranged by size:
2702 2165 1158 711
BsaBI GATnn'nnATC
Cuts at: 6730 6730
Size: 6736
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4543 4925 5560 275
Size: 53 83 186 936 3010 382 635 1451
Fragments arranged by size:
3010 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5169 5567 5841 5988 6399 5169
Size: 398 274 147 411 5506
Fragments arranged by size:
5506 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3387 3417 1178
Size: 30 165 30 1984 30 4497
Fragments arranged by size:
4497 1984 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2919 3926 1186
Size: 1733 1007 3996
Fragments arranged by size:
3996 1733 1007
BseYI C'CCAG_C
Cuts at: 2408 5939 6435 2408
Size: 3531 496 2709
Fragments arranged by size:
3531 2709 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2069 3524 1163
Size: 573 333 1455 4375
Fragments arranged by size:
4375 1455 573 333
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2152 4186 4947 5975 1540
Size: 275 186 151 2034 761 1028 2301
Fragments arranged by size:
2301 2034 1028 761 275 186 151
BsiHKAI G_wGCw'C
Cuts at: 4797 4882 5953 4797
Size: 85 1071 5580
Fragments arranged by size:
5580 1071 85
BsmI GAATG_Cn'
Cuts at: 2317 4247 4340 2317
Size: 1930 93 4713
Fragments arranged by size:
4713 1930 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2631 3534 4055 4797 4882 5953 1391
Size: 399 841 903 521 742 85 1071 2174
Fragments arranged by size:
2174 1071 903 841 742 521 399 85
BspEI T'CCGG_A
Cuts at: 6399 6399
Size: 6736
BspHI T'CATG_A
Cuts at: 4625 6355 4625
Size: 1730 5006
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2041 4091 844
Size: 1197 2050 3489
Fragments arranged by size:
3489 2050 1197
BsrBI CCG'CTC
Cuts at: 1394 1823 3996 4623 1394
Size: 429 2173 627 3507
Fragments arranged by size:
3507 2173 627 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2648 2681 5231 5405 65
Size: 1183 1400 33 2550 174 1396
Fragments arranged by size:
2550 1400 1396 1183 174 33
BsrFI r'CCGG_y
Cuts at: 1700 1810 2019 2147 5380 5567 1700
Size: 110 209 128 3233 187 2869
Fragments arranged by size:
3233 2869 209 187 128 110
BsrGI T'GTAC_A
Cuts at: 96 2837 3029 3053 96
Size: 2741 192 24 3779
Fragments arranged by size:
3779 2741 192 24
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6736
BssSI C'ACGA_G
Cuts at: 4796 5808 4796
Size: 1012 5724
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2462 2676 3344 794
Size: 1668 214 668 4186
Fragments arranged by size:
4186 1668 668 214
BstBI TT'CG_AA
Cuts at: 3967 5540 3967
Size: 1573 5163
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6736
BstXI CCAn_nnnn'nTGG
Cuts at: 3482 3482
Size: 6736
BstYI r'GATC_y
Cuts at: 1972 2119 2197 2590 3193 3373 3949 4066 4433
Size: 147 78 393 603 180 576 117 367
Cuts at: 4433 4818 4835 6287 6373 6385 6731 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 603 576 393 385 367 346
180 147 117 86 78 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 4032 513
Size: 552 939 2028 3217
Fragments arranged by size:
3217 2028 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5997
Fragments arranged by size:
5997 683 56
BtsI GCAGTG_nn'
Cuts at: 4252 5046 5066 4252
Size: 794 20 5922
Fragments arranged by size:
5922 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6736
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6701
DraI TTT'AAA
Cuts at: 3238 4040 3238
Size: 802 5934
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5743 809
Size: 507 4427 1802
Fragments arranged by size:
4427 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2526 2722 4183
Size: 54 1425 50 275 18 696 196 1461
Cuts at: 4183 5072 6701 8
Size: 889 1629 43
Fragments arranged by size:
1629 1461 1425 889 696 275 196 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4183 1537
Size: 275 2371 4090
Fragments arranged by size:
4090 2371 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3450 3856 3986 4666 1353
Size: 2097 406 130 680 3423
Fragments arranged by size:
3423 2097 680 406 130
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2053 5323 5697 5843 387
Size: 1064 138 464 3270 374 146 1280
Fragments arranged by size:
3270 1280 1064 464 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3030 3585 3711 4799 5396 6183 6451
Size: 393 1398 555 126 1088 597 787 268
Cuts at: 6451 1239
Size: 1524
Fragments arranged by size:
1524 1398 1088 787 597 555 393 268
126
EcoRI G'AATT_C
Cuts at: 2988 4045 4423 2988
Size: 1057 378 5301
Fragments arranged by size:
5301 1057 378
FspI TGC'GCA
Cuts at: 5242 5242
Size: 6736
HaeII r_GCGC'y
Cuts at: 1678 2777 5883 1678
Size: 1099 3106 2531
Fragments arranged by size:
3106 2531 1099
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2145 2167 2177 2199 3387
Size: 32 135 32 573 22 10 22 1188
Cuts at: 3387 3419 3602 3634 3743 3775 3818 3850 5377
Size: 32 183 32 109 32 43 32 1527
Cuts at: 5377 5409 5451 5483 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1527 1188 573 183 135 109 43
42 32 32 32 32 32 32 32
32 22 22 10
HincII GTy'rAC
Cuts at: 669 1657 1938 2337 3291 4080 4326 669
Size: 988 281 399 954 789 246 3079
Fragments arranged by size:
3079 988 954 789 399 281 246
HindIII A'AGCT_T
Cuts at: 748 2618 748
Size: 1870 4866
HpaI GTT'AAC
Cuts at: 3291 4326 3291
Size: 1035 5701
KpnI G_GTAC'C
Cuts at: 4061 4061
Size: 6736
MfeI C'AATT_G
Cuts at: 4335 4335
Size: 6736
MluI A'CGCG_T
Cuts at: 6612 6612
Size: 6736
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2394 3736 5850 6034 1305
Size: 1089 1342 2114 184 2007
Fragments arranged by size:
2114 2007 1342 1089 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2528 2724 10
Size: 54 1425 343 696 196 4022
Fragments arranged by size:
4022 1425 696 343 196 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2801 2864 3480 3665 3804 4694 5053
Size: 648 1635 63 616 185 139 890 359
Cuts at: 5053 5212 518
Size: 159 2042
Fragments arranged by size:
2042 1635 890 648 616 359 185 159
139 63
MspA1I CmG'CkG
Cuts at: 1606 2116 2408 2921 4152 4829 5977 6222 1606
Size: 510 292 513 1231 677 1148 245 2120
Fragments arranged by size:
2120 1231 1148 677 513 510 292 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5245
Fragments arranged by size:
5245 939 552
NdeI CA'TA_TG
Cuts at: 387 2377 3986 387
Size: 1990 1609 3137
Fragments arranged by size:
3137 1990 1609
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6736
NotI GC'GGCC_GC
Cuts at: 4183 4183
Size: 6736
NsiI A_TGCA'T
Cuts at: 3724 3724
Size: 6736
NspI r_CATG'y
Cuts at: 1132 1229 2335 2604 2804 3183 3440 3775 3803
Size: 97 1106 269 200 379 257 335 28
Cuts at: 3803 4094 5639 1132
Size: 291 1545 2229
Fragments arranged by size:
2229 1545 1106 379 335 291 269 257
200 97 28
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6736
PciI A'CATG_T
Cuts at: 2600 2800 3436 3799 5635 2600
Size: 200 636 363 1836 3701
Fragments arranged by size:
3701 1836 636 363 200
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3946 1108
Size: 2838 3898
PfoI T'CCnGG_A
Cuts at: 3414 3414
Size: 6736
PmeI GTTT'AAAC
Cuts at: 4040 4040
Size: 6736
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4805 4837 6318 6350 4805
Size: 32 1481 32 5191
Fragments arranged by size:
5191 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6736
PsiI TTA'TAA
Cuts at: 4306 4306
Size: 6736
PstI C_TGCA'G
Cuts at: 830 2115 4088 830
Size: 1285 1973 3478
Fragments arranged by size:
3478 1973 1285
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6736
PvuII CAG'CTG
Cuts at: 2116 2408 2921 2116
Size: 292 513 5931
Fragments arranged by size:
5931 513 292
SalI G'TCGA_C
Cuts at: 1936 4078 1936
Size: 2142 4594
SapI GCTCTTCn'nnn_
Cuts at: 3856 3986 3856
Size: 130 6606
SbfI CC_TGCA'GG
Cuts at: 4088 4088
Size: 6736
ScaI AGT'ACT
Cuts at: 4984 6696 4984
Size: 1712 5024
SexAI A'CCwGG_T
Cuts at: 3066 3939 3066
Size: 873 5863
SfcI C'TryA_G
Cuts at: 826 1045 2111 2961 4084 5219 5900 6091 826
Size: 219 1066 850 1123 1135 681 191 1471
Fragments arranged by size:
1471 1135 1123 1066 850 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6736
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6736
SmaI CCC'GGG
Cuts at: 1872 4063 1872
Size: 2191 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2897 4840 5741 6003 820
Size: 197 926 954 1943 901 262 1553
Fragments arranged by size:
1943 1553 954 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2362 4527 493
Size: 1869 2165 2702
Fragments arranged by size:
2702 2165 1869
SpeI A'CTAG_T
Cuts at: 152 2395 152
Size: 2243 4493
SphI G_CATG'C
Cuts at: 1132 4094 1132
Size: 2962 3774
SspI AAT'ATT
Cuts at: 5 52 2427 3422 4660 5
Size: 47 2375 995 1238 2081
Fragments arranged by size:
2375 2081 1238 995 47
StuI AGG'CCT
Cuts at: 1517 2369 1517
Size: 852 5884
StyI C'CwwG_G
Cuts at: 513 1065 2004 2382 2719 4175 513
Size: 552 939 378 337 1456 3074
Fragments arranged by size:
3074 1456 939 552 378 337
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4763 4780 4933 5118 5563 325
Size: 1255 3183 17 153 185 445 1498
Fragments arranged by size:
3183 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2085 2248 2837
Size: 274 80 33 51 1443 108 163 589
Cuts at: 2837 3029 3053 4982 6694 96
Size: 192 24 1929 1712 138
Fragments arranged by size:
1929 1712 1443 589 274 192 163 138
108 80 51 33 24
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3982 4014 6696 6728 3982
Size: 32 2682 32 3990
Fragments arranged by size:
3990 2682 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3616 1316
Size: 2300 4436
XbaI T'CTAG_A
Cuts at: 4072 4072
Size: 6736
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2521 2536 3151 2521
Size: 15 615 6106
Fragments arranged by size:
6106 615 15
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6736
XmaI C'CCGG_G
Cuts at: 1870 4061 1870
Size: 2191 4545
XmnI GAAnn'nnTTC
Cuts at: 3980 4865 3980
Size: 885 5851
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4544 276
Size: 53 83 186 936 3010 2468
Fragments arranged by size:
3010 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI
SwaI