(Circular) (Six-base) MAPSORT of: pFN21AA1430.seq Check: 1795 from: 1 to: 6301 pFN21AA1430 6301 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4111 278 Size: 53 83 186 936 2575 2468 Fragments arranged by size: 2575 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3644 1937 Size: 1707 4594 Acc65I G'GTAC_C Cuts at: 3622 3622 Size: 6301 AclI AA'CG_TT Cuts at: 1563 2475 3373 4428 4801 1563 Size: 912 898 1055 373 3063 Fragments arranged by size: 3063 1055 912 898 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2532 2769 2786 2961 3096 4364 5748 6016 Size: 900 237 17 175 135 1268 1384 268 Cuts at: 6016 1632 Size: 1917 Fragments arranged by size: 1917 1384 1268 900 268 237 175 135 17 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6104 AflIII A'CryG_T Cuts at: 1203 2341 2343 2632 5200 6177 1203 Size: 1138 2 289 2568 977 1327 Fragments arranged by size: 2568 1327 1138 977 289 2 AgeI A'CCGG_T Cuts at: 5132 5132 Size: 6301 AhdI GACnn_n'nnGTC Cuts at: 5030 5030 Size: 6301 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6301 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6301 AlwNI CAG_nnn'CTG Cuts at: 2582 4044 5616 2582 Size: 1462 1572 3267 Fragments arranged by size: 3267 1572 1462 ApaLI G'TGCA_C Cuts at: 4358 5514 4358 Size: 1156 5145 ApoI r'AATT_y Cuts at: 1493 2158 2225 2361 3610 3836 3988 6097 1493 Size: 665 67 136 1249 226 152 2109 1697 Fragments arranged by size: 2109 1697 1249 665 226 152 136 67 AseI AT'TA_AT Cuts at: 160 1032 4855 160 Size: 872 3823 1606 Fragments arranged by size: 3823 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6301 AvaI C'yCGr_G Cuts at: 1870 1943 3122 3626 1870 Size: 73 1179 504 4545 Fragments arranged by size: 4545 1179 504 73 BamHI G'GATC_C Cuts at: 3631 3998 3631 Size: 367 5934 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3622 5077 618 Size: 325 217 627 1835 1455 1842 Fragments arranged by size: 1842 1835 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3620 1391 Size: 2229 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2967 3571 928 Size: 971 1068 604 3658 Fragments arranged by size: 3658 1068 971 604 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4492 4526 1903 Size: 34 2555 34 3678 Fragments arranged by size: 3678 2555 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4193 5403 4193 Size: 1210 5091 BclI T'GATC_A Cuts at: 1548 2830 3103 3250 1548 Size: 1282 273 147 4599 Fragments arranged by size: 4599 1282 273 147 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4912 136 Size: 107 122 71 631 605 3240 1525 Fragments arranged by size: 3240 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6296 6296 Size: 6301 BlpI GC'TnA_GC Cuts at: 3718 3718 Size: 6301 Bme1580I G_kGCm'C Cuts at: 1790 4362 5518 1790 Size: 2572 1156 2573 Fragments arranged by size: 2573 2572 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6301 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2825 4032 4990 Size: 416 53 83 399 24 1408 1207 958 Cuts at: 4990 442 Size: 1753 Fragments arranged by size: 1753 1408 1207 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6301 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2769 2974 3529 4961 1239 Size: 1530 205 555 1432 2579 Fragments arranged by size: 2579 1530 1432 555 205 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3362 4426 5291 5589 3362 Size: 1064 865 298 4074 Fragments arranged by size: 4074 1064 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3512 4964 882 Size: 617 2013 1452 2219 Fragments arranged by size: 2219 2013 1452 617 BsaAI yAC'GTr Cuts at: 493 1204 2344 4092 493 Size: 711 1140 1748 2702 Fragments arranged by size: 2702 1748 1140 711 BsaBI GATnn'nnATC Cuts at: 2589 3042 6295 2589 Size: 453 3253 2595 Fragments arranged by size: 3253 2595 453 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4108 4490 5125 275 Size: 53 83 186 936 2575 382 635 1451 Fragments arranged by size: 2575 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4734 5132 5406 5553 5964 4734 Size: 398 274 147 411 5071 Fragments arranged by size: 5071 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6076 Fragments arranged by size: 6076 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2506 2515 2518 2756 2953 1186 Size: 1320 9 3 238 197 4534 Fragments arranged by size: 4534 1320 238 197 9 3 BseYI C'CCAG_C Cuts at: 5504 6000 5504 Size: 496 5805 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5728 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3169 3751 4512 5540 1540 Size: 275 186 1168 582 761 1028 2301 Fragments arranged by size: 2301 1168 1028 761 582 275 186 BsiHKAI G_wGCw'C Cuts at: 3280 4362 4447 5518 3280 Size: 1082 85 1071 4063 Fragments arranged by size: 4063 1082 1071 85 BsmI GAATG_Cn' Cuts at: 2178 3812 3905 2178 Size: 1634 93 4574 Fragments arranged by size: 4574 1634 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2610 2610 Size: 6301 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3280 3620 4362 4447 5518 1391 Size: 399 1490 340 742 85 1071 2174 Fragments arranged by size: 2174 1490 1071 742 399 340 85 BspEI T'CCGG_A Cuts at: 5964 5964 Size: 6301 BspHI T'CATG_A Cuts at: 4190 5920 4190 Size: 1730 4571 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3656 844 Size: 2812 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4188 1394 Size: 429 2365 3507 Fragments arranged by size: 3507 2365 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2282 4796 4970 65 Size: 1183 1034 2514 174 1396 Fragments arranged by size: 2514 1396 1183 1034 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4945 5132 1700 Size: 110 3135 187 2869 Fragments arranged by size: 3135 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6301 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6301 BssSI C'ACGA_G Cuts at: 4361 5373 4361 Size: 1012 5289 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6301 BstBI TT'CG_AA Cuts at: 2162 5105 2162 Size: 2943 3358 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6301 BstYI r'GATC_y Cuts at: 1972 3037 3446 3631 3998 4383 4400 5852 5938 Size: 1065 409 185 367 385 17 1452 86 Cuts at: 5938 5950 6296 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1065 409 385 367 346 185 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2739 2739 Size: 6301 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4810 Fragments arranged by size: 4810 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3383 487 Size: 683 56 2157 3405 Fragments arranged by size: 3405 2157 683 56 BtsI GCAGTG_nn' Cuts at: 2425 3817 4611 4631 2425 Size: 1392 794 20 4095 Fragments arranged by size: 4095 1392 794 20 ClaI AT'CG_AT Cuts at: 1554 3165 1554 Size: 1611 4690 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6266 DraI TTT'AAA Cuts at: 3605 3605 Size: 6301 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5308 809 Size: 507 3992 1802 Fragments arranged by size: 3992 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2728 3457 3550 Size: 54 1425 50 275 18 898 729 93 Cuts at: 3550 3748 4637 6266 8 Size: 198 889 1629 43 Fragments arranged by size: 1629 1425 898 889 729 275 198 93 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3748 1537 Size: 275 1936 4090 Fragments arranged by size: 4090 1936 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2299 2524 2805 3144 4231 1353 Size: 946 225 281 339 1087 3423 Fragments arranged by size: 3423 1087 946 339 281 225 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3224 4888 5262 5408 387 Size: 1064 138 1635 1664 374 146 1280 Fragments arranged by size: 1664 1635 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2532 2769 2786 2961 2974 3096 3529 Size: 393 900 237 17 175 13 122 433 Cuts at: 3529 4364 4961 5748 6016 1239 Size: 835 597 787 268 1524 Fragments arranged by size: 1524 900 835 787 597 433 393 268 237 175 122 17 13 EcoRI G'AATT_C Cuts at: 2361 3610 3988 2361 Size: 1249 378 4674 Fragments arranged by size: 4674 1249 378 FspI TGC'GCA Cuts at: 4807 4807 Size: 6301 HaeII r_GCGC'y Cuts at: 1678 5448 1678 Size: 3770 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2564 2596 2948 2980 3379 Size: 32 135 32 992 32 352 32 399 Cuts at: 3379 3411 4942 4974 5016 5048 1373 Size: 32 1531 32 42 32 2626 Fragments arranged by size: 2626 1531 992 399 352 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3538 3645 3891 669 Size: 988 281 1600 107 246 3079 Fragments arranged by size: 3079 1600 988 281 246 107 HindIII A'AGCT_T Cuts at: 748 3057 748 Size: 2309 3992 HpaI GTT'AAC Cuts at: 3891 3891 Size: 6301 KpnI G_GTAC'C Cuts at: 3626 3626 Size: 6301 MfeI C'AATT_G Cuts at: 3900 3900 Size: 6301 MluI A'CGCG_T Cuts at: 6177 6177 Size: 6301 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5415 5599 1305 Size: 4110 184 2007 Fragments arranged by size: 4110 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2730 3459 3552 10 Size: 54 1425 343 898 729 93 2759 Fragments arranged by size: 2759 1425 898 729 343 93 54 MslI CAynn'nnrTG Cuts at: 518 1166 2171 2722 4259 4618 4777 518 Size: 648 1005 551 1537 359 159 2042 Fragments arranged by size: 2042 1537 1005 648 551 359 159 MspA1I CmG'CkG Cuts at: 1606 3533 3717 4394 5542 5787 1606 Size: 1927 184 677 1148 245 2120 Fragments arranged by size: 2120 1927 1148 677 245 184 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4810 Fragments arranged by size: 4810 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6301 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6301 NotI GC'GGCC_GC Cuts at: 3748 3748 Size: 6301 NspI r_CATG'y Cuts at: 1132 1229 2636 3659 5204 1132 Size: 97 1407 1023 1545 2229 Fragments arranged by size: 2229 1545 1407 1023 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6301 PciI A'CATG_T Cuts at: 2632 5200 2632 Size: 2568 3733 PflMI CCAn_nnn'nTGG Cuts at: 1108 2726 3362 1108 Size: 1618 636 4047 Fragments arranged by size: 4047 1618 636 PfoI T'CCnGG_A Cuts at: 3482 3482 Size: 6301 PmeI GTTT'AAAC Cuts at: 3605 3605 Size: 6301 PmlI CAC'GTG Cuts at: 2344 2344 Size: 6301 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4370 4402 5883 5915 4370 Size: 32 1481 32 4756 Fragments arranged by size: 4756 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2769 1613 Size: 1156 5145 PsiI TTA'TAA Cuts at: 3871 3871 Size: 6301 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3358 3390 3358 Size: 32 6269 PstI C_TGCA'G Cuts at: 830 3653 830 Size: 2823 3478 PvuI CG_AT'CG Cuts at: 2001 3169 2001 Size: 1168 5133 SalI G'TCGA_C Cuts at: 1936 3643 1936 Size: 1707 4594 SbfI CC_TGCA'GG Cuts at: 3653 3653 Size: 6301 ScaI AGT'ACT Cuts at: 2713 4549 6261 2713 Size: 1836 1712 2753 Fragments arranged by size: 2753 1836 1712 SfcI C'TryA_G Cuts at: 826 1045 3649 4784 5465 5656 826 Size: 219 2604 1135 681 191 1471 Fragments arranged by size: 2604 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6301 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6301 SmaI CCC'GGG Cuts at: 1872 3124 3628 1872 Size: 1252 504 4545 Fragments arranged by size: 4545 1252 504 SmlI C'TyrA_G Cuts at: 820 1017 1943 3341 4405 5306 5568 820 Size: 197 926 1398 1064 901 262 1553 Fragments arranged by size: 1553 1398 1064 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4092 493 Size: 3599 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6301 SphI G_CATG'C Cuts at: 1132 3659 1132 Size: 2527 3774 SspI AAT'ATT Cuts at: 5 52 2915 4225 5 Size: 47 2863 1310 2081 Fragments arranged by size: 2863 2081 1310 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6301 StyI C'CwwG_G Cuts at: 513 1065 2004 3740 513 Size: 552 939 1736 3074 Fragments arranged by size: 3074 1736 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4328 4345 4498 4683 5128 325 Size: 1255 2748 17 153 185 445 1498 Fragments arranged by size: 2748 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2711 3230 4547 Size: 274 80 33 51 1443 734 519 1317 Cuts at: 4547 6259 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1317 734 519 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3478 3510 6261 6293 3478 Size: 32 2751 32 3486 Fragments arranged by size: 3486 2751 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6301 XbaI T'CTAG_A Cuts at: 3637 3637 Size: 6301 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6301 XmaI C'CCGG_G Cuts at: 1870 3122 3626 1870 Size: 1252 504 4545 Fragments arranged by size: 4545 1252 504 XmnI GAAnn'nnTTC Cuts at: 4430 4430 Size: 6301 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4109 276 Size: 53 83 186 936 2575 2468 Fragments arranged by size: 2575 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PsrI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI