(Circular) (Six-base) MAPSORT of: pFN21AA1426.seq Check: 3974 from: 1 to: 7900 pFN21AA1426 7900 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3307 4047 3307 Size: 740 7160 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4608 5710 278 Size: 53 83 186 936 3072 1102 2468 Fragments arranged by size: 3072 2468 1102 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3465 5243 1937 Size: 1528 1778 4594 Fragments arranged by size: 4594 1778 1528 Acc65I G'GTAC_C Cuts at: 4848 5221 4848 Size: 373 7527 AclI AA'CG_TT Cuts at: 1563 4104 6027 6400 1563 Size: 2541 1923 373 3063 Fragments arranged by size: 3063 2541 1923 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2123 2303 3614 4332 5963 7347 7615 1632 Size: 491 180 1311 718 1631 1384 268 1917 Fragments arranged by size: 1917 1631 1384 1311 718 491 268 180 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7703 AflIII A'CryG_T Cuts at: 1203 6799 7776 1203 Size: 5596 977 1327 Fragments arranged by size: 5596 1327 977 AgeI A'CCGG_T Cuts at: 6731 6731 Size: 7900 AhdI GACnn_n'nnGTC Cuts at: 6629 6629 Size: 7900 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7900 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2424 4979 1184 Size: 1240 2555 4105 Fragments arranged by size: 4105 2555 1240 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3737 3769 3737 Size: 32 7868 AlwNI CAG_nnn'CTG Cuts at: 2122 2734 3973 5643 7215 2122 Size: 612 1239 1670 1572 2807 Fragments arranged by size: 2807 1670 1572 1239 612 ApaI G_GGCC'C Cuts at: 2281 2395 3522 2281 Size: 114 1127 6659 Fragments arranged by size: 6659 1127 114 ApaLI G'TGCA_C Cuts at: 2639 2663 5957 7113 2639 Size: 24 3294 1156 3426 Fragments arranged by size: 3426 3294 1156 24 ApoI r'AATT_y Cuts at: 1493 2471 5209 5435 5587 7696 1493 Size: 978 2738 226 152 2109 1697 Fragments arranged by size: 2738 2109 1697 978 226 152 AseI AT'TA_AT Cuts at: 160 1032 6454 160 Size: 872 5422 1606 Fragments arranged by size: 5422 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7900 AvaI C'yCGr_G Cuts at: 1870 1943 2094 2522 2763 2876 2899 2997 3113 Size: 73 151 428 241 113 23 98 116 Cuts at: 3113 3241 3254 4372 5225 1870 Size: 128 13 1118 853 4545 Fragments arranged by size: 4545 1118 853 428 241 151 128 116 113 98 73 23 13 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4840 4873 4840 Size: 33 7867 BamHI G'GATC_C Cuts at: 5230 5597 5230 Size: 367 7533 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2937 3835 4035 4364 4848 Size: 325 217 627 1150 898 200 329 484 Cuts at: 4848 5221 6676 618 Size: 373 1455 1842 Fragments arranged by size: 1842 1455 1150 898 627 484 373 329 325 217 200 BanII G_rGCy'C Cuts at: 1391 2281 2395 2849 3057 3261 3312 3329 3522 Size: 890 114 454 208 204 51 17 193 Cuts at: 3522 4433 4499 4580 5219 1391 Size: 911 66 81 639 4072 Fragments arranged by size: 4072 911 890 639 454 208 204 193 114 81 66 51 17 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2522 2972 4164 4338 928 Size: 971 623 450 1192 174 4490 Fragments arranged by size: 4490 1192 971 623 450 174 BbvCI CC'TCA_GC Cuts at: 2796 3581 2796 Size: 785 7115 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3883 3917 6091 6125 1903 Size: 34 1946 34 2174 34 3678 Fragments arranged by size: 3678 2174 1946 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5792 7002 5792 Size: 1210 6690 BclI T'GATC_A Cuts at: 1548 2080 1548 Size: 532 7368 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6511 136 Size: 107 122 71 631 605 4839 1525 Fragments arranged by size: 4839 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7895 7895 Size: 7900 BlpI GC'TnA_GC Cuts at: 3402 4877 5130 5317 3402 Size: 1475 253 187 5985 Fragments arranged by size: 5985 1475 253 187 Bme1580I G_kGCm'C Cuts at: 1790 2281 2395 2643 2667 2759 3522 4212 5961 Size: 491 114 248 24 92 763 690 1749 Cuts at: 5961 7117 1790 Size: 1156 2573 Fragments arranged by size: 2573 1749 1156 763 690 491 248 114 92 24 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7900 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2958 3933 4152 Size: 416 53 83 399 24 1541 975 219 Cuts at: 4152 4837 4848 5631 6589 442 Size: 685 11 783 958 1753 Fragments arranged by size: 1753 1541 975 958 783 685 416 399 219 83 53 24 11 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7900 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3834 4430 3834 Size: 596 7304 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2443 3065 3296 3521 3832 4523 4564 4731 Size: 1204 622 231 225 311 691 41 167 Cuts at: 4731 6560 1239 Size: 1829 2579 Fragments arranged by size: 2579 1829 1204 691 622 311 231 225 167 41 Bpu10I CC'TnA_GC Cuts at: 2796 2894 3581 4464 2796 Size: 98 687 883 6232 Fragments arranged by size: 6232 883 687 98 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2329 2981 6025 6890 7188 2329 Size: 652 3044 865 298 3041 Fragments arranged by size: 3044 3041 865 652 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2461 6563 882 Size: 617 962 4102 2219 Fragments arranged by size: 4102 2219 962 617 BsaAI yAC'GTr Cuts at: 493 1204 2713 5691 493 Size: 711 1509 2978 2702 Fragments arranged by size: 2978 2702 1509 711 BsaBI GATnn'nnATC Cuts at: 4706 7894 4706 Size: 3188 4712 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2706 2835 3692 4605 Size: 53 83 186 936 1173 129 857 913 Cuts at: 4605 5707 6089 6724 275 Size: 1102 382 635 1451 Fragments arranged by size: 1451 1173 1102 936 913 857 635 382 186 129 83 53 BsaWI w'CCGG_w Cuts at: 2849 4715 6333 6731 7005 7152 7563 2849 Size: 1866 1618 398 274 147 411 3186 Fragments arranged by size: 3186 1866 1618 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2534 2564 3668 3698 3737 Size: 30 165 30 1131 30 1104 30 39 Cuts at: 3737 3767 1178 Size: 30 5311 Fragments arranged by size: 5311 1131 1104 165 39 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2540 2919 3320 3431 3569 3692 3790 4861 Size: 1354 379 401 111 138 123 98 1071 Cuts at: 4861 1186 Size: 4225 Fragments arranged by size: 4225 1354 1071 401 379 138 123 111 98 BseYI C'CCAG_C Cuts at: 2722 3485 3514 3782 4806 4973 5049 7103 7599 Size: 763 29 268 1024 167 76 2054 496 Cuts at: 7599 2722 Size: 3023 Fragments arranged by size: 3023 2054 1024 763 496 268 167 76 29 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2420 2564 2641 3774 1163 Size: 573 684 144 77 1133 5289 Fragments arranged by size: 5289 1133 684 573 144 77 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2683 3526 4202 4206 5350 6111 Size: 275 186 682 843 676 4 1144 761 Cuts at: 6111 7139 1540 Size: 1028 2301 Fragments arranged by size: 2301 1144 1028 843 761 682 676 275 186 4 BsiHKAI G_wGCw'C Cuts at: 2643 2667 2849 3057 3312 4580 5961 6046 7117 Size: 24 182 208 255 1268 1381 85 1071 Cuts at: 7117 2643 Size: 3426 Fragments arranged by size: 3426 1381 1268 1071 255 208 182 85 24 BsmI GAATG_Cn' Cuts at: 2191 5411 5504 2191 Size: 3220 93 4587 Fragments arranged by size: 4587 3220 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3351 4612 3351 Size: 1261 6639 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2281 2395 2643 2667 2759 2849 3057 Size: 399 491 114 248 24 92 90 208 Cuts at: 3057 3261 3312 3329 3522 4212 4433 4499 4580 Size: 204 51 17 193 690 221 66 81 Cuts at: 4580 5219 5961 6046 7117 1391 Size: 639 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 690 639 491 399 248 221 208 204 193 114 92 90 85 81 66 51 24 17 BspEI T'CCGG_A Cuts at: 2849 7563 2849 Size: 4714 3186 BspHI T'CATG_A Cuts at: 3471 5789 7519 3471 Size: 2318 1730 3852 Fragments arranged by size: 3852 2318 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2014 2259 3307 3438 4047 5255 844 Size: 1170 245 1048 131 609 1208 3489 Fragments arranged by size: 3489 1208 1170 1048 609 245 131 BsrBI CCG'CTC Cuts at: 1394 1823 2530 2790 5787 1394 Size: 429 707 260 2997 3507 Fragments arranged by size: 3507 2997 707 429 260 BsrDI GCAATG_nn' Cuts at: 65 1248 3868 6395 6569 65 Size: 1183 2620 2527 174 1396 Fragments arranged by size: 2620 2527 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2682 3393 4032 4277 6544 6731 1700 Size: 110 872 711 639 245 2267 187 2869 Fragments arranged by size: 2869 2267 872 711 639 245 187 110 BsrGI T'GTAC_A Cuts at: 96 2603 96 Size: 2507 5393 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7900 BssSI C'ACGA_G Cuts at: 4234 5960 6972 4234 Size: 1726 1012 5162 Fragments arranged by size: 5162 1726 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7900 BstBI TT'CG_AA Cuts at: 6704 6704 Size: 7900 BstEII G'GTnAC_C Cuts at: 1184 3709 1184 Size: 2525 5375 BstXI CCAn_nnnn'nTGG Cuts at: 2948 3397 4981 5020 2948 Size: 449 1584 39 5828 Fragments arranged by size: 5828 1584 449 39 BstYI r'GATC_y Cuts at: 1972 2730 5230 5597 5982 5999 7451 7537 7549 Size: 758 2500 367 385 17 1452 86 12 Cuts at: 7549 7895 1972 Size: 346 1977 Fragments arranged by size: 2500 1977 1452 758 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2426 3802 4644 2426 Size: 1376 842 5682 Fragments arranged by size: 5682 1376 842 BtgI C'CryG_G Cuts at: 513 1065 2004 2372 2783 3330 4422 513 Size: 552 939 368 411 547 1092 3991 Fragments arranged by size: 3991 1092 939 552 547 411 368 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 5110 487 Size: 683 56 3884 3277 Fragments arranged by size: 3884 3277 683 56 BtsI GCAGTG_nn' Cuts at: 2576 3125 4067 4771 5416 6210 6230 2576 Size: 549 942 704 645 794 20 4246 Fragments arranged by size: 4246 942 794 704 645 549 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7900 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4088 4123 529 Size: 35 3524 35 4306 Fragments arranged by size: 4306 3524 35 35 DraI TTT'AAA Cuts at: 5204 5204 Size: 7900 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3107 4442 6907 809 Size: 507 1791 1335 2465 1802 Fragments arranged by size: 2465 1802 1791 1335 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2680 3028 3456 Size: 54 1425 50 275 18 850 348 428 Cuts at: 3456 3523 4199 4245 5137 5347 6236 7865 8 Size: 67 676 46 892 210 889 1629 43 Fragments arranged by size: 1629 1425 892 889 850 676 428 348 275 210 67 54 50 46 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2680 3523 4199 5347 1537 Size: 275 868 843 676 1148 4090 Fragments arranged by size: 4090 1148 868 843 676 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2313 3935 4138 4907 5830 1353 Size: 960 1622 203 769 923 3423 Fragments arranged by size: 3423 1622 960 923 769 203 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2018 2260 3330 3953 6487 6861 Size: 1064 138 429 242 1070 623 2534 374 Cuts at: 6861 7007 387 Size: 146 1280 Fragments arranged by size: 2534 1280 1070 1064 623 429 374 242 146 138 EcoICRI GAG'CTC Cuts at: 2847 3055 3310 4578 2847 Size: 208 255 1268 6169 Fragments arranged by size: 6169 1268 255 208 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2123 2303 2443 3065 3296 3521 3614 Size: 393 491 180 140 622 231 225 93 Cuts at: 3614 3832 4332 4523 4564 4731 5963 6560 7347 Size: 218 500 191 41 167 1232 597 787 Cuts at: 7347 7615 1239 Size: 268 1524 Fragments arranged by size: 1524 1232 787 622 597 500 491 393 268 231 225 218 191 180 167 140 93 41 EcoNI CCTnn'n_nnAGG Cuts at: 2976 3661 5195 2976 Size: 685 1534 5681 Fragments arranged by size: 5681 1534 685 EcoO109I rG'GnC_Cy Cuts at: 2278 3009 3100 3335 3369 3382 3518 3532 4216 Size: 731 91 235 34 13 136 14 684 Cuts at: 4216 4531 5016 2278 Size: 315 485 5162 Fragments arranged by size: 5162 731 684 485 315 235 136 91 34 14 13 EcoRI G'AATT_C Cuts at: 2471 5209 5587 2471 Size: 2738 378 4784 Fragments arranged by size: 4784 2738 378 FspI TGC'GCA Cuts at: 2153 6406 2153 Size: 4253 3647 HaeII r_GCGC'y Cuts at: 1678 2267 2495 2874 3577 7047 1678 Size: 589 228 379 703 3470 2531 Fragments arranged by size: 3470 2531 703 589 379 228 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2534 2566 3041 3073 3668 Size: 32 135 32 962 32 475 32 595 Cuts at: 3668 3700 4310 4342 4640 4672 4810 4842 4976 Size: 32 610 32 298 32 138 32 134 Cuts at: 4976 5008 6541 6573 6615 6647 1373 Size: 32 1533 32 42 32 2626 Fragments arranged by size: 2626 1533 962 610 595 475 298 138 135 134 42 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3616 5244 5490 669 Size: 988 281 1678 1628 246 3079 Fragments arranged by size: 3079 1678 1628 988 281 246 HindIII A'AGCT_T Cuts at: 748 4894 748 Size: 4146 3754 HpaI GTT'AAC Cuts at: 5490 5490 Size: 7900 KpnI G_GTAC'C Cuts at: 4852 5225 4852 Size: 373 7527 MfeI C'AATT_G Cuts at: 5499 5499 Size: 7900 MluI A'CGCG_T Cuts at: 7776 7776 Size: 7900 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3975 4458 4962 7014 7198 1305 Size: 2670 483 504 2052 184 2007 Fragments arranged by size: 2670 2052 2007 504 483 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3030 4247 10 Size: 54 1425 343 1198 1217 3663 Fragments arranged by size: 3663 1425 1217 1198 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3986 4401 5858 6217 6376 518 Size: 648 2820 415 1457 359 159 2042 Fragments arranged by size: 2820 2042 1457 648 415 359 159 MspA1I CmG'CkG Cuts at: 1606 2374 2536 2617 2785 3126 3332 3499 3585 Size: 768 162 81 168 341 206 167 86 Cuts at: 3585 3735 4561 5053 5180 5316 5993 7141 7386 Size: 150 826 492 127 136 677 1148 245 Cuts at: 7386 1606 Size: 2120 Fragments arranged by size: 2120 1148 826 768 677 492 341 245 206 168 167 162 150 136 127 86 81 NaeI GCC'GGC Cuts at: 3395 4279 3395 Size: 884 7016 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 6409 Fragments arranged by size: 6409 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7900 NgoMIV G'CCGG_C Cuts at: 3393 4277 3393 Size: 884 7016 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7900 NotI GC'GGCC_GC Cuts at: 5347 5347 Size: 7900 NspI r_CATG'y Cuts at: 1132 1229 5258 6803 1132 Size: 97 4029 1545 2229 Fragments arranged by size: 4029 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3698 1348 Size: 2350 5550 PciI A'CATG_T Cuts at: 6799 6799 Size: 7900 PflMI CCAn_nnn'nTGG Cuts at: 1108 2734 1108 Size: 1626 6274 PfoI T'CCnGG_A Cuts at: 3312 4289 3312 Size: 977 6923 PmeI GTTT'AAAC Cuts at: 5204 5204 Size: 7900 PmlI CAC'GTG Cuts at: 2713 2713 Size: 7900 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3737 3769 5969 6001 7482 7514 3737 Size: 32 2200 32 1481 32 4123 Fragments arranged by size: 4123 2200 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3100 3382 3532 4216 5016 3100 Size: 282 150 684 800 5984 Fragments arranged by size: 5984 800 684 282 150 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7900 PsiI TTA'TAA Cuts at: 5470 5470 Size: 7900 PspOMI G'GGCC_C Cuts at: 2277 2391 3518 2277 Size: 114 1127 6659 Fragments arranged by size: 6659 1127 114 PspXI vC'TCGA_Gb Cuts at: 2522 2899 2522 Size: 377 7523 PstI C_TGCA'G Cuts at: 830 2541 2977 3435 3498 5252 830 Size: 1711 436 458 63 1754 3478 Fragments arranged by size: 3478 1754 1711 458 436 63 PvuI CG_AT'CG Cuts at: 2001 4206 2001 Size: 2205 5695 PvuII CAG'CTG Cuts at: 2536 3499 3585 5053 2536 Size: 963 86 1468 5383 Fragments arranged by size: 5383 1468 963 86 SacI G_AGCT'C Cuts at: 2849 3057 3312 4580 2849 Size: 208 255 1268 6169 Fragments arranged by size: 6169 1268 255 208 SacII CC_GC'GG Cuts at: 2375 2786 3333 2375 Size: 411 547 6942 Fragments arranged by size: 6942 547 411 SalI G'TCGA_C Cuts at: 1936 5242 1936 Size: 3306 4594 SapI GCTCTTCn'nnn_ Cuts at: 3935 4907 3935 Size: 972 6928 SbfI CC_TGCA'GG Cuts at: 5252 5252 Size: 7900 ScaI AGT'ACT Cuts at: 6148 7860 6148 Size: 1712 6188 SfcI C'TryA_G Cuts at: 826 1045 2358 2366 2537 2973 3431 3494 3651 Size: 219 1313 8 171 436 458 63 157 Cuts at: 3651 5248 6383 7064 7255 826 Size: 1597 1135 681 191 1471 Fragments arranged by size: 1597 1471 1313 1135 681 458 436 219 191 171 157 63 8 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7900 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7900 SmaI CCC'GGG Cuts at: 1872 2765 2878 2999 3256 5227 1872 Size: 893 113 121 257 1971 4545 Fragments arranged by size: 4545 1971 893 257 121 113 SmlI C'TyrA_G Cuts at: 820 1017 1943 2094 2308 2522 2899 2960 6004 Size: 197 926 151 214 214 377 61 3044 Cuts at: 6004 6905 7167 820 Size: 901 262 1553 Fragments arranged by size: 3044 1553 926 901 377 262 214 214 197 151 61 SnaBI TAC'GTA Cuts at: 493 5691 493 Size: 5198 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7900 SphI G_CATG'C Cuts at: 1132 5258 1132 Size: 4126 3774 SspI AAT'ATT Cuts at: 5 52 5824 5 Size: 47 5772 2081 Fragments arranged by size: 5772 2081 47 StuI AGG'CCT Cuts at: 1517 2100 2651 2926 3752 3879 4462 1517 Size: 583 551 275 826 127 583 4955 Fragments arranged by size: 4955 826 583 583 551 275 127 StyI C'CwwG_G Cuts at: 513 1065 2004 2646 2700 4212 4308 4527 5339 Size: 552 939 642 54 1512 96 219 812 Cuts at: 5339 513 Size: 3074 Fragments arranged by size: 3074 1512 939 812 642 552 219 96 54 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3922 4847 5927 5944 6097 6282 6727 Size: 1255 2342 925 1080 17 153 185 445 Cuts at: 6727 325 Size: 1498 Fragments arranged by size: 2342 1498 1255 1080 925 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2067 2603 6146 Size: 274 80 33 51 1443 90 536 3543 Cuts at: 6146 7858 96 Size: 1712 138 Fragments arranged by size: 3543 1712 1443 536 274 138 90 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4041 4073 4936 4968 7860 7892 4041 Size: 32 863 32 2892 32 4049 Fragments arranged by size: 4049 2892 863 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7900 XbaI T'CTAG_A Cuts at: 3064 5236 3064 Size: 2172 5728 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3628 4240 3628 Size: 612 7288 XhoI C'TCGA_G Cuts at: 1943 2094 2522 2899 1943 Size: 151 428 377 6944 Fragments arranged by size: 6944 428 377 151 XmaI C'CCGG_G Cuts at: 1870 2763 2876 2997 3254 5225 1870 Size: 893 113 121 257 1971 4545 Fragments arranged by size: 4545 1971 893 257 121 113 XmnI GAAnn'nnTTC Cuts at: 2208 3929 6029 2208 Size: 1721 2100 4079 Fragments arranged by size: 4079 2100 1721 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4606 5708 276 Size: 53 83 186 936 3072 1102 2468 Fragments arranged by size: 3072 2468 1102 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BbeI BfrBI BsiWI BstZ17I DraIII EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PsrI RsrII SanDI SexAI SfiI SfoI SrfI SwaI