Restriction map for
pFN21AA1386
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# NO CUT ENZYMES ( 112 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Aor51HI
ApaI AscI AseI AsnI Asp718I
AspEI AviII AvrII BanI BbeI
BcgI BciVI BclI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaHI
BsaI BsaWI BseAI BsiEI BsiWI
BsmBI BspDI BspEI BspLU11I BspMI
BsrBI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I CelII Cfr10I ClaI
CpoI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I FbaI FseI FspI
HaeII HgaI Hin1I HincII HindII
HpaI KasI KpnI KspI MluI
MroI MvnI NaeI NarI NcoI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PinAI PmeI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SwaI XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AflIII 1716 ACACGT A^C[GA][CT]GT
Alw44I 229 GTGCAC G^TGCAC
ApaLI 229 GTGCAC G^TGCAC
BalI 2877 TGGCCA TGG^CCA
BamHI 615 GGATCC G^GATCC
BbrPI 1717 CACGTG CAC^GTG
BbvCI 82 CCTCAGC CCTCAGC(-5/-2)
BcnI 828 CCCGG CC^[GC]GG
BsaAI 1717 CACGTG [CT]AC^GT[GA]
BsmI 1973 GCATTC GAATGC(1/-1)
Bsp1407I 2148 TGTACA T^GTACA
BsrDI 1346 CATTGC GCAATG(2/0)
BsrGI 2148 TGTACA T^GTACA
DraI 1813 TTTAAA TTT^AAA
DraIII 875 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2318 GACAACGTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 2438 CCGTGG C^C[GA][CT]GG
EaeI 2877 TGGCCA [CT]^GGCC[GA]
EcoNI 1267 CCTACTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 2167 GAATTC G^AATTC
EcoT22I 247 ATGCAT ATGCA^T
HapII 829 CCGG C^CGG
HpaII 829 CCGG C^CGG
MluNI 2877 TGGCCA TGG^CCA
MscI 2877 TGGCCA TGG^CCA
MspA1I 2873 CAGCTG C[AC]G^C[GT]G
MspI 829 CCGG C^CGG
NciI 828 CCCGG CC^[GC]GG
NsiI 247 ATGCAT ATGCA^T
PmaCI 1717 CACGTG CAC^GTG
PmlI 1717 CACGTG CAC^GTG
PvuII 2873 CAGCTG CAG^CTG
SphI 2140 GCATGC GCATG^C
SspBI 2148 TGTACA T^GTACA
XcmI 1865 CCAAAGAAAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 25 enzymes) #
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AccI 701 GTAGAC GT^[AC][GT]AC
2345 GTCTAC
AlwNI 2363 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
2530 CAGGCTCTG
Asp700I 1705 GAAGTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3130 GAAAATATTC
AspI 2318 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
2689 GACTCTGTC
AvaII 265 GGACC G^G[AT]CC
2883 GGACC
BsaBI 205 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2267 GATTTGCATC
CfoI 1971 GCGC GCG^C
2226 GCGC
DraII 264 GGGACCT [GA]G^G[ACGT]CC[CT]
2882 AGGACCC
EcoO109I 264 GGGACCT [GA]G^G[ACGT]CC[CT]
2882 AGGACCC
EcoRV 279 GATATC GAT^ATC
1168 GATATC
FauI 2117 CCCGC CCCGC(4/6)
3142 GCGGG
HhaI 1971 GCGC GCG^C
2226 GCGC
HinP1I 1971 GCGC G^CGC
2226 GCGC
HindIII 767 AAGCTT A^AGCTT
812 AAGCTT
MaeII 1718 ACGT A^CGT
2322 ACGT
MamI 205 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2267 GATTTGCATC
MfeI 1521 CAATTG C^AATTG
1882 CAATTG
MslI 1002 CATTTGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2433 CACATCCGTG
MunI 1521 CAATTG C^AATTG
1882 CAATTG
PflFI 2318 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
2689 GACTCTGTC
PflMI 874 CCACCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1253 CCATTAAATGG
PpuMI 264 GGGACCT [GA]G^G[AT]CC[CT]
2882 AGGACCC
Tth111I 2318 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
2689 GACTCTGTC
Van91I 874 CCACCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1253 CCATTAAATGG
XmnI 1705 GAAGTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3130 GAAAATATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AocI ApoI AspHI AvaI BanII
BbsI BbvI BfaI BmyI BpmI
BpuAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspHI BsrI BssKI BstF5I
BstNI BstYI Bsu36I Cac8I Cfr13I
DdeI DpnII EarI Eco81I EcoRII
EcoT14I Fnu4HI FokI HaeIII HinfI
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MvaI MwoI NdeI NdeII NlaIII
NlaIV NspI PleI PstI RcaI
RsaI SacI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII