(Circular) (Six-base) MAPSORT of: pFN21AA1386.seq Check: 9992 from: 1 to: 7972 pFN21AA1386 7972 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5782 278 Size: 53 83 186 936 4246 2468 Fragments arranged by size: 4246 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2707 4351 5315 1937 Size: 770 1644 964 4594 Fragments arranged by size: 4594 1644 964 770 Acc65I G'GTAC_C Cuts at: 5293 5293 Size: 7972 AclI AA'CG_TT Cuts at: 1563 6099 6472 1563 Size: 4536 373 3063 Fragments arranged by size: 4536 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2310 2652 2732 2790 3933 4098 4517 6035 Size: 678 342 80 58 1143 165 419 1518 Cuts at: 6035 7419 7687 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1518 1384 1143 678 419 342 268 165 80 58 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7775 AflIII A'CryG_T Cuts at: 1203 3721 6871 7848 1203 Size: 2518 3150 977 1327 Fragments arranged by size: 3150 2518 1327 977 AgeI A'CCGG_T Cuts at: 6803 6803 Size: 7972 AhdI GACnn_n'nnGTC Cuts at: 6701 6701 Size: 7972 AleI CACnn'nnGTG Cuts at: 1166 4442 1166 Size: 3276 4696 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2475 3166 3623 1184 Size: 1291 691 457 5533 Fragments arranged by size: 5533 1291 691 457 AlwNI CAG_nnn'CTG Cuts at: 4373 4540 5715 7287 4373 Size: 167 1175 1572 5058 Fragments arranged by size: 5058 1572 1175 167 ApaLI G'TGCA_C Cuts at: 2234 6029 7185 2234 Size: 3795 1156 3021 Fragments arranged by size: 3795 3021 1156 ApoI r'AATT_y Cuts at: 1493 2024 3105 4038 4172 4298 5281 5507 5659 Size: 531 1081 933 134 126 983 226 152 Cuts at: 5659 7768 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1081 983 933 531 226 152 134 126 AseI AT'TA_AT Cuts at: 160 1032 6526 160 Size: 872 5494 1606 Fragments arranged by size: 5494 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7972 AvaI C'yCGr_G Cuts at: 1870 1943 2624 4191 4722 5297 1870 Size: 73 681 1567 531 575 4545 Fragments arranged by size: 4545 1567 681 575 531 73 BamHI G'GATC_C Cuts at: 2620 5302 5669 2620 Size: 2682 367 4923 Fragments arranged by size: 4923 2682 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 5293 6748 618 Size: 325 217 627 3506 1455 1842 Fragments arranged by size: 3506 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2133 2299 2788 3364 3901 4820 5291 1391 Size: 742 166 489 576 537 919 471 4072 Fragments arranged by size: 4072 919 742 576 537 489 471 166 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3042 4086 4563 928 Size: 971 1143 1044 477 4337 Fragments arranged by size: 4337 1143 1044 971 477 BbvCI CC'TCA_GC Cuts at: 2088 2088 Size: 7972 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6163 6197 1903 Size: 34 4226 34 3678 Fragments arranged by size: 4226 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5864 7074 5864 Size: 1210 6762 BclI T'GATC_A Cuts at: 1548 1548 Size: 7972 BfrBI ATG'CAT Cuts at: 2254 2254 Size: 7972 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6583 136 Size: 107 122 71 631 605 4911 1525 Fragments arranged by size: 4911 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7967 7967 Size: 7972 BlpI GC'TnA_GC Cuts at: 5389 5389 Size: 7972 Bme1580I G_kGCm'C Cuts at: 1790 2238 6033 7189 1790 Size: 448 3795 1156 2573 Fragments arranged by size: 3795 2573 1156 448 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7972 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2803 5703 6661 Size: 416 53 83 399 24 1386 2900 958 Cuts at: 6661 442 Size: 1753 Fragments arranged by size: 2900 1753 1386 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7972 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3915 4655 3915 Size: 740 7232 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3261 3702 4350 4956 6632 1239 Size: 2022 441 648 606 1676 2579 Fragments arranged by size: 2579 2022 1676 648 606 441 Bpu10I CC'TnA_GC Cuts at: 2088 4814 2088 Size: 2726 5246 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3968 5148 5224 6097 6962 7260 3968 Size: 1180 76 873 865 298 4680 Fragments arranged by size: 4680 1180 873 865 298 76 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6635 882 Size: 617 5136 2219 Fragments arranged by size: 5136 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3724 5763 493 Size: 711 2520 2039 2702 Fragments arranged by size: 2702 2520 2039 711 BsaBI GATnn'nnATC Cuts at: 2214 4276 7966 2214 Size: 2062 3690 2220 Fragments arranged by size: 3690 2220 2062 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5779 6161 6796 275 Size: 53 83 186 936 4246 382 635 1451 Fragments arranged by size: 4246 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6405 6803 7077 7224 7635 6405 Size: 398 274 147 411 6742 Fragments arranged by size: 6742 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7747 Fragments arranged by size: 7747 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2523 2745 3743 4327 4350 5214 1186 Size: 1337 222 998 584 23 864 3944 Fragments arranged by size: 3944 1337 998 864 584 222 23 BseYI C'CCAG_C Cuts at: 3399 5001 7175 7671 3399 Size: 1602 2174 496 3700 Fragments arranged by size: 3700 2174 1602 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3037 4457 5013 1163 Size: 573 1301 1420 556 4122 Fragments arranged by size: 4122 1420 1301 573 556 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5422 6183 7211 1540 Size: 275 186 3421 761 1028 2301 Fragments arranged by size: 3421 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2238 2788 3364 3901 3963 4820 6033 6118 7189 Size: 550 576 537 62 857 1213 85 1071 Cuts at: 7189 2238 Size: 3021 Fragments arranged by size: 3021 1213 1071 857 576 550 537 85 62 BsmI GAATG_Cn' Cuts at: 3978 5483 5576 3978 Size: 1505 93 6374 Fragments arranged by size: 6374 1505 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2133 2238 2299 2788 3364 3901 3963 Size: 399 343 105 61 489 576 537 62 Cuts at: 3963 4820 5291 6033 6118 7189 1391 Size: 857 471 742 85 1071 2174 Fragments arranged by size: 2174 1071 857 742 576 537 489 471 399 343 105 85 62 61 BspEI T'CCGG_A Cuts at: 7635 7635 Size: 7972 BspHI T'CATG_A Cuts at: 2788 4770 4936 5861 7591 2788 Size: 1982 166 925 1730 3169 Fragments arranged by size: 3169 1982 1730 925 166 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5327 844 Size: 4483 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5859 1394 Size: 429 4036 3507 Fragments arranged by size: 4036 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3350 6467 6641 65 Size: 1183 2102 3117 174 1396 Fragments arranged by size: 3117 2102 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6616 6803 1700 Size: 110 4806 187 2869 Fragments arranged by size: 4806 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 4153 96 Size: 4057 3915 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7972 BssSI C'ACGA_G Cuts at: 6032 7044 6032 Size: 1012 6960 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7972 BstBI TT'CG_AA Cuts at: 6776 6776 Size: 7972 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7972 BstYI r'GATC_y Cuts at: 1972 2620 2698 3329 5302 5669 6054 6071 7523 Size: 648 78 631 1973 367 385 17 1452 Cuts at: 7523 7609 7621 7967 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1973 1452 648 631 385 367 346 86 78 17 12 Bsu36I CC'TnA_GG Cuts at: 2467 2694 4657 2467 Size: 227 1963 5782 Fragments arranged by size: 5782 1963 227 BtgI C'CryG_G Cuts at: 513 1065 2004 4443 513 Size: 552 939 2439 4042 Fragments arranged by size: 4042 2439 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7233 Fragments arranged by size: 7233 683 56 BtsI GCAGTG_nn' Cuts at: 3617 4592 5488 6282 6302 3617 Size: 975 896 794 20 5287 Fragments arranged by size: 5287 975 896 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7972 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3922 3957 529 Size: 35 3358 35 4544 Fragments arranged by size: 4544 3358 35 35 DraI TTT'AAA Cuts at: 3820 5276 3820 Size: 1456 6516 DraIII CAC_nnn'GTG Cuts at: 2885 2885 Size: 7972 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4329 6979 809 Size: 507 3013 2650 1802 Fragments arranged by size: 3013 2650 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4882 5419 6308 Size: 54 1425 50 275 18 3052 537 889 Cuts at: 6308 7937 8 Size: 1629 43 Fragments arranged by size: 3052 1629 1425 889 537 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5419 1537 Size: 275 3607 4090 Fragments arranged by size: 4090 3607 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2335 2947 3398 3648 4157 4486 4536 5902 Size: 982 612 451 250 509 329 50 1366 Cuts at: 5902 1353 Size: 3423 Fragments arranged by size: 3423 1366 982 612 509 451 329 250 50 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6559 6933 7079 387 Size: 1064 138 4970 374 146 1280 Fragments arranged by size: 4970 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2786 3362 3899 4818 2786 Size: 576 537 919 5940 Fragments arranged by size: 5940 919 576 537 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2310 2652 2732 2790 3261 3702 3933 Size: 393 678 342 80 58 471 441 231 Cuts at: 3933 4098 4350 4517 4956 6035 6632 7419 7687 Size: 165 252 167 439 1079 597 787 268 Cuts at: 7687 1239 Size: 1524 Fragments arranged by size: 1524 1079 787 678 597 471 441 439 393 342 268 252 231 167 165 80 58 EcoNI CCTnn'n_nnAGG Cuts at: 3276 3276 Size: 7972 EcoO109I rG'GnC_Cy Cuts at: 2270 4888 2270 Size: 2618 5354 EcoRI G'AATT_C Cuts at: 4172 5281 5659 4172 Size: 1109 378 6485 Fragments arranged by size: 6485 1109 378 EcoRV GAT'ATC Cuts at: 2286 3175 2286 Size: 889 7083 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2120 2315 2738 2795 3649 2120 Size: 195 423 57 854 6443 Fragments arranged by size: 6443 854 423 195 57 FspI TGC'GCA Cuts at: 6478 6478 Size: 7972 HaeII r_GCGC'y Cuts at: 1678 7119 1678 Size: 5441 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3719 3751 4859 4891 6613 Size: 32 135 32 2147 32 1108 32 1722 Cuts at: 6613 6645 6687 6719 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2147 1722 1108 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5316 5562 669 Size: 988 281 3378 246 3079 Fragments arranged by size: 3378 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 2772 2817 748 Size: 2024 45 5903 Fragments arranged by size: 5903 2024 45 HpaI GTT'AAC Cuts at: 5562 5562 Size: 7972 KpnI G_GTAC'C Cuts at: 5297 5297 Size: 7972 MfeI C'AATT_G Cuts at: 3526 3887 5571 3526 Size: 361 1684 5927 Fragments arranged by size: 5927 1684 361 MluI A'CGCG_T Cuts at: 7848 7848 Size: 7972 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2853 3181 7086 7270 1305 Size: 1548 328 3905 184 2007 Fragments arranged by size: 3905 2007 1548 328 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4884 10 Size: 54 1425 343 3052 3098 Fragments arranged by size: 3098 3052 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3011 4442 5930 6289 6448 518 Size: 648 1845 1431 1488 359 159 2042 Fragments arranged by size: 2042 1845 1488 1431 648 359 159 MspA1I CmG'CkG Cuts at: 1606 4880 5388 6065 7213 7458 1606 Size: 3274 508 677 1148 245 2120 Fragments arranged by size: 3274 2120 1148 677 508 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 6481 Fragments arranged by size: 6481 939 552 NdeI CA'TA_TG Cuts at: 387 2677 3145 4000 387 Size: 2290 468 855 4359 Fragments arranged by size: 4359 2290 855 468 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7972 NotI GC'GGCC_GC Cuts at: 5419 5419 Size: 7972 NsiI A_TGCA'T Cuts at: 2256 2256 Size: 7972 NspI r_CATG'y Cuts at: 1132 1229 2746 3610 4149 5330 6875 1132 Size: 97 1517 864 539 1181 1545 2229 Fragments arranged by size: 2229 1545 1517 1181 864 539 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7972 PciI A'CATG_T Cuts at: 6871 6871 Size: 7972 PflMI CCAn_nnn'nTGG Cuts at: 1108 2885 3264 1108 Size: 1777 379 5816 Fragments arranged by size: 5816 1777 379 PmeI GTTT'AAAC Cuts at: 5276 5276 Size: 7972 PmlI CAC'GTG Cuts at: 3724 3724 Size: 7972 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4326 4358 6041 6073 7554 7586 4326 Size: 32 1683 32 1481 32 4712 Fragments arranged by size: 4712 1683 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2270 4888 2270 Size: 2618 5354 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7972 PsiI TTA'TAA Cuts at: 5542 5542 Size: 7972 PstI C_TGCA'G Cuts at: 830 2358 2740 4210 5324 830 Size: 1528 382 1470 1114 3478 Fragments arranged by size: 3478 1528 1470 1114 382 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7972 PvuII CAG'CTG Cuts at: 4880 4880 Size: 7972 SacI G_AGCT'C Cuts at: 2788 3364 3901 4820 2788 Size: 576 537 919 5940 Fragments arranged by size: 5940 919 576 537 SalI G'TCGA_C Cuts at: 1936 5314 1936 Size: 3378 4594 SapI GCTCTTCn'nnn_ Cuts at: 2335 2947 4486 2335 Size: 612 1539 5821 Fragments arranged by size: 5821 1539 612 SbfI CC_TGCA'GG Cuts at: 5324 5324 Size: 7972 ScaI AGT'ACT Cuts at: 6220 7932 6220 Size: 1712 6260 SfcI C'TryA_G Cuts at: 826 1045 2354 2736 3218 4206 4980 5320 6455 Size: 219 1309 382 482 988 774 340 1135 Cuts at: 6455 7136 7327 826 Size: 681 191 1471 Fragments arranged by size: 1471 1309 1135 988 774 681 482 382 340 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7972 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7972 SmaI CCC'GGG Cuts at: 1872 5299 1872 Size: 3427 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3983 5163 5239 6076 6977 7239 Size: 197 926 2040 1180 76 837 901 262 Cuts at: 7239 820 Size: 1553 Fragments arranged by size: 2040 1553 1180 926 901 837 262 197 76 SnaBI TAC'GTA Cuts at: 493 5763 493 Size: 5270 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7972 SphI G_CATG'C Cuts at: 1132 4149 5330 1132 Size: 3017 1181 3774 Fragments arranged by size: 3774 3017 1181 SspI AAT'ATT Cuts at: 5 52 2187 4265 5140 5896 5 Size: 47 2135 2078 875 756 2081 Fragments arranged by size: 2135 2081 2078 875 756 47 StuI AGG'CCT Cuts at: 1517 2692 3236 4105 4432 1517 Size: 1175 544 869 327 5057 Fragments arranged by size: 5057 1175 869 544 327 StyI C'CwwG_G Cuts at: 513 1065 2004 2927 3409 3738 4226 4548 5411 Size: 552 939 923 482 329 488 322 863 Cuts at: 5411 513 Size: 3074 Fragments arranged by size: 3074 939 923 863 552 488 482 329 322 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5999 6016 6169 6354 6799 325 Size: 1255 4419 17 153 185 445 1498 Fragments arranged by size: 4419 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3698 4153 5260 Size: 274 80 33 51 1443 1721 455 1107 Cuts at: 5260 6218 7930 96 Size: 958 1712 138 Fragments arranged by size: 1721 1712 1443 1107 958 455 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3330 3362 4466 4498 7932 7964 3330 Size: 32 1104 32 3434 32 3338 Fragments arranged by size: 3434 3338 1104 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4326 4697 1316 Size: 3010 371 4591 Fragments arranged by size: 4591 3010 371 XbaI T'CTAG_A Cuts at: 5308 5308 Size: 7972 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3877 3877 Size: 7972 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7972 XmaI C'CCGG_G Cuts at: 1870 5297 1870 Size: 3427 4545 XmnI GAAnn'nnTTC Cuts at: 3714 5139 6101 3714 Size: 1425 962 5585 Fragments arranged by size: 5585 1425 962 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5780 276 Size: 53 83 186 936 4246 2468 Fragments arranged by size: 4246 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BsiWI BsmBI BstXI BstZ17I FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI