(Circular) (Six-base) MAPSORT of: pFN21AA1351.seq Check: 4162 from: 1 to: 8377 pFN21AA1351 8377 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6187 278 Size: 53 83 186 936 4651 2468 Fragments arranged by size: 4651 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2920 5720 1937 Size: 983 2800 4594 Fragments arranged by size: 4594 2800 983 Acc65I G'GTAC_C Cuts at: 5698 5698 Size: 8377 AclI AA'CG_TT Cuts at: 1563 6504 6877 1563 Size: 4941 373 3063 Fragments arranged by size: 4941 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2732 3064 4347 6440 7824 8092 1632 Size: 1100 332 1283 2093 1384 268 1917 Fragments arranged by size: 2093 1917 1384 1283 1100 332 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8180 AflIII A'CryG_T Cuts at: 1203 2781 2947 7276 8253 1203 Size: 1578 166 4329 977 1327 Fragments arranged by size: 4329 1578 1327 977 166 AgeI A'CCGG_T Cuts at: 7208 7208 Size: 8377 AhdI GACnn_n'nnGTC Cuts at: 7106 7106 Size: 8377 AleI CACnn'nnGTG Cuts at: 1166 3549 1166 Size: 2383 5994 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 5257 1184 Size: 4073 4304 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3181 3213 3181 Size: 32 8345 AlwNI CAG_nnn'CTG Cuts at: 2594 3463 5017 5053 6120 7692 2594 Size: 869 1554 36 1067 1572 3279 Fragments arranged by size: 3279 1572 1554 1067 869 36 ApaI G_GGCC'C Cuts at: 2059 2059 Size: 8377 ApaLI G'TGCA_C Cuts at: 3353 6434 7590 3353 Size: 3081 1156 4140 Fragments arranged by size: 4140 3081 1156 ApoI r'AATT_y Cuts at: 1493 2691 2723 3181 3240 4195 4201 4678 4721 Size: 1198 32 458 59 955 6 477 43 Cuts at: 4721 4937 5686 5912 6064 8173 1493 Size: 216 749 226 152 2109 1697 Fragments arranged by size: 2109 1697 1198 955 749 477 458 226 216 152 59 43 32 6 AseI AT'TA_AT Cuts at: 160 1032 4301 6931 160 Size: 872 3269 2630 1606 Fragments arranged by size: 3269 2630 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8377 AvaI C'yCGr_G Cuts at: 1870 1943 2799 3906 4029 4869 5702 1870 Size: 73 856 1107 123 840 833 4545 Fragments arranged by size: 4545 1107 856 840 833 123 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4158 4191 4158 Size: 33 8344 BamHI G'GATC_C Cuts at: 5707 6074 5707 Size: 367 8010 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2450 2663 4530 4740 5698 Size: 325 217 627 663 213 1867 210 958 Cuts at: 5698 7153 618 Size: 1455 1842 Fragments arranged by size: 1867 1842 1455 958 663 627 325 217 213 210 BanII G_rGCy'C Cuts at: 1391 2059 5696 1391 Size: 668 3637 4072 Fragments arranged by size: 4072 3637 668 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 7406 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6568 6602 1903 Size: 34 4631 34 3678 Fragments arranged by size: 4631 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4237 4330 6269 7479 4237 Size: 93 1939 1210 5135 Fragments arranged by size: 5135 1939 1210 93 BclI T'GATC_A Cuts at: 1548 3972 4987 1548 Size: 2424 1015 4938 Fragments arranged by size: 4938 2424 1015 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6988 136 Size: 107 122 71 631 605 5316 1525 Fragments arranged by size: 5316 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2957 8372 2957 Size: 5415 2962 BlpI GC'TnA_GC Cuts at: 5794 5794 Size: 8377 Bme1580I G_kGCm'C Cuts at: 1790 2059 3357 4535 6438 7594 1790 Size: 269 1298 1178 1903 1156 2573 Fragments arranged by size: 2573 1903 1298 1178 1156 269 BmgBI CAC'GTC Cuts at: 1318 4909 1318 Size: 3591 4786 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2098 6108 7066 Size: 416 53 83 399 24 681 4010 958 Cuts at: 7066 442 Size: 1753 Fragments arranged by size: 4010 1753 958 681 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8377 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5612 5612 Size: 8377 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3800 7037 1239 Size: 2561 3237 2579 Fragments arranged by size: 3237 2579 2561 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2688 2843 3143 3344 5471 5666 6502 7367 7665 Size: 155 300 201 2127 195 836 865 298 Cuts at: 7665 2688 Size: 3400 Fragments arranged by size: 3400 2127 865 836 300 298 201 195 155 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 7040 882 Size: 617 5541 2219 Fragments arranged by size: 5541 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2948 6168 493 Size: 711 1744 3220 2702 Fragments arranged by size: 3220 2702 1744 711 BsaBI GATnn'nnATC Cuts at: 2810 3003 8371 2810 Size: 193 5368 2816 Fragments arranged by size: 5368 2816 193 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5238 6184 6566 7201 Size: 53 83 186 936 3705 946 382 635 Cuts at: 7201 275 Size: 1451 Fragments arranged by size: 3705 1451 946 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6810 7208 7482 7629 8040 6810 Size: 398 274 147 411 7147 Fragments arranged by size: 7147 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4314 4344 4834 4864 1178 Size: 30 165 30 2911 30 490 30 4691 Fragments arranged by size: 4691 2911 490 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4652 4857 5327 5387 1186 Size: 3466 205 470 60 4176 Fragments arranged by size: 4176 3466 470 205 60 BseYI C'CCAG_C Cuts at: 2632 3314 7580 8076 2632 Size: 682 4266 496 2933 Fragments arranged by size: 4266 2933 682 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4766 4796 5066 5088 1163 Size: 573 3030 30 270 22 4452 Fragments arranged by size: 4452 3030 573 270 30 22 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5827 6588 7616 1540 Size: 275 186 3826 761 1028 2301 Fragments arranged by size: 3826 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2433 3357 4111 4914 5150 6438 6523 7594 2433 Size: 924 754 803 236 1288 85 1071 3216 Fragments arranged by size: 3216 1288 1071 924 803 754 236 85 BsmI GAATG_Cn' Cuts at: 2443 3115 5888 5981 2443 Size: 672 2773 93 4839 Fragments arranged by size: 4839 2773 672 93 BsmBI CGTCTCn'nnnn_ Cuts at: 5423 5423 Size: 8377 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2059 2433 3357 4111 4535 4914 5150 Size: 399 269 374 924 754 424 379 236 Cuts at: 5150 5696 6438 6523 7594 1391 Size: 546 742 85 1071 2174 Fragments arranged by size: 2174 1071 924 754 742 546 424 399 379 374 269 236 85 BspEI T'CCGG_A Cuts at: 8040 8040 Size: 8377 BspHI T'CATG_A Cuts at: 3144 6266 7996 3144 Size: 3122 1730 3525 Fragments arranged by size: 3525 3122 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2769 4154 4818 5259 5732 844 Size: 1925 1385 664 441 473 3489 Fragments arranged by size: 3489 1925 1385 664 473 441 BsrBI CCG'CTC Cuts at: 1394 1823 6264 1394 Size: 429 4441 3507 Fragments arranged by size: 4441 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3675 3706 4314 6872 7046 65 Size: 1183 2427 31 608 2558 174 1396 Fragments arranged by size: 2558 2427 1396 1183 608 174 31 BsrFI r'CCGG_y Cuts at: 1700 1810 7021 7208 1700 Size: 110 5211 187 2869 Fragments arranged by size: 5211 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 4311 5003 96 Size: 4215 692 3470 Fragments arranged by size: 4215 3470 692 BssHII G'CGCG_C Cuts at: 1925 2039 1925 Size: 114 8263 BssSI C'ACGA_G Cuts at: 6437 7449 6437 Size: 1012 7365 BstAPI GCAn_nnn'nTGC Cuts at: 794 5552 794 Size: 4758 3619 BstBI TT'CG_AA Cuts at: 2953 7181 2953 Size: 4228 4149 BstEII G'GTnAC_C Cuts at: 1184 3858 1184 Size: 2674 5703 BstXI CCAn_nnnn'nTGG Cuts at: 2237 5158 2237 Size: 2921 5456 BstYI r'GATC_y Cuts at: 1972 2328 2957 2998 3004 5707 6074 6459 6476 Size: 356 629 41 6 2703 367 385 17 Cuts at: 6476 7928 8014 8026 8372 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 2703 1977 1452 629 385 367 356 346 86 41 17 12 6 Bsu36I CC'TnA_GG Cuts at: 2587 5310 2587 Size: 2723 5654 BtgI C'CryG_G Cuts at: 513 1065 3915 513 Size: 552 2850 4975 Fragments arranged by size: 4975 2850 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4135 487 Size: 683 56 2909 4729 Fragments arranged by size: 4729 2909 683 56 BtsI GCAGTG_nn' Cuts at: 3461 5532 5893 6687 6707 3461 Size: 2071 361 794 20 5131 Fragments arranged by size: 5131 2071 794 361 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8377 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8342 DraI TTT'AAA Cuts at: 4200 5681 4200 Size: 1481 6896 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7384 809 Size: 507 6068 1802 Fragments arranged by size: 6068 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2649 4060 5824 Size: 54 1425 50 275 18 819 1411 1764 Cuts at: 5824 6713 8342 8 Size: 889 1629 43 Fragments arranged by size: 1764 1629 1425 1411 889 819 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5824 1537 Size: 275 4012 4090 Fragments arranged by size: 4090 4012 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2169 2462 3872 5667 6307 1353 Size: 816 293 1410 1795 640 3423 Fragments arranged by size: 3423 1795 1410 816 640 293 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6964 7338 7484 387 Size: 1064 138 5375 374 146 1280 Fragments arranged by size: 5375 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2732 3064 3800 4347 6440 7037 7824 Size: 393 1100 332 736 547 2093 597 787 Cuts at: 7824 8092 1239 Size: 268 1524 Fragments arranged by size: 2093 1524 1100 787 736 597 547 393 332 268 EcoNI CCTnn'n_nnAGG Cuts at: 2586 4284 2586 Size: 1698 6679 EcoO109I rG'GnC_Cy Cuts at: 2055 2056 2591 4554 5135 2055 Size: 1 535 1963 581 5297 Fragments arranged by size: 5297 1963 581 535 1 EcoRI G'AATT_C Cuts at: 2723 3240 4678 4721 5686 6064 2723 Size: 517 1438 43 965 378 5036 Fragments arranged by size: 5036 1438 965 517 378 43 FspI TGC'GCA Cuts at: 6883 6883 Size: 8377 HaeII r_GCGC'y Cuts at: 1678 7524 1678 Size: 5846 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5633 5665 7018 7050 7092 Size: 32 135 32 4061 32 1353 32 42 Cuts at: 7092 7124 1373 Size: 32 2626 Fragments arranged by size: 4061 2626 1353 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3432 4515 4630 4980 5344 5721 Size: 988 281 1494 1083 115 350 364 377 Cuts at: 5721 5967 669 Size: 246 3079 Fragments arranged by size: 3079 1494 1083 988 377 364 350 281 246 115 HindIII A'AGCT_T Cuts at: 748 5478 748 Size: 4730 3647 HpaI GTT'AAC Cuts at: 4515 5967 4515 Size: 1452 6925 KpnI G_GTAC'C Cuts at: 5702 5702 Size: 8377 MfeI C'AATT_G Cuts at: 3309 5976 3309 Size: 2667 5710 MluI A'CGCG_T Cuts at: 8253 8253 Size: 8377 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3686 5037 7491 7675 1305 Size: 2381 1351 2454 184 2007 Fragments arranged by size: 2454 2381 2007 1351 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2651 4062 10 Size: 54 1425 343 819 1411 4325 Fragments arranged by size: 4325 1425 1411 819 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2928 3449 3549 3719 6335 6694 6853 Size: 648 1762 521 100 170 2616 359 159 Cuts at: 6853 518 Size: 2042 Fragments arranged by size: 2616 2042 1762 648 521 359 170 159 100 MspA1I CmG'CkG Cuts at: 1606 5621 5793 6470 7618 7863 1606 Size: 4015 172 677 1148 245 2120 Fragments arranged by size: 4015 2120 1148 677 245 172 NcoI C'CATG_G Cuts at: 513 1065 3915 513 Size: 552 2850 4975 Fragments arranged by size: 4975 2850 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8377 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8377 NotI GC'GGCC_GC Cuts at: 5824 5824 Size: 8377 NspI r_CATG'y Cuts at: 1132 1229 2342 2785 5735 7280 1132 Size: 97 1113 443 2950 1545 2229 Fragments arranged by size: 2950 2229 1545 1113 443 97 PasI CC'CwG_GG Cuts at: 1348 2594 1348 Size: 1246 7131 PciI A'CATG_T Cuts at: 2781 7276 2781 Size: 4495 3882 PflMI CCAn_nnn'nTGG Cuts at: 1108 2460 4124 1108 Size: 1352 1664 5361 Fragments arranged by size: 5361 1664 1352 PfoI T'CCnGG_A Cuts at: 2352 2352 Size: 8377 PmeI GTTT'AAAC Cuts at: 5681 5681 Size: 8377 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6446 6478 7959 7991 6446 Size: 32 1481 32 6832 Fragments arranged by size: 6832 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3932 1613 Size: 2319 6058 PsiI TTA'TAA Cuts at: 2964 5947 2964 Size: 2983 5394 PspOMI G'GGCC_C Cuts at: 2055 2055 Size: 8377 PstI C_TGCA'G Cuts at: 830 3169 3987 5236 5256 5729 830 Size: 2339 818 1249 20 473 3478 Fragments arranged by size: 3478 2339 1249 818 473 20 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8377 PvuII CAG'CTG Cuts at: 5621 5621 Size: 8377 SalI G'TCGA_C Cuts at: 1936 5719 1936 Size: 3783 4594 SapI GCTCTTCn'nnn_ Cuts at: 2462 3872 2462 Size: 1410 6967 SbfI CC_TGCA'GG Cuts at: 5729 5729 Size: 8377 ScaI AGT'ACT Cuts at: 6625 8337 6625 Size: 1712 6665 SfcI C'TryA_G Cuts at: 826 1045 3165 3246 3257 3983 4961 5232 5252 Size: 219 2120 81 11 726 978 271 20 Cuts at: 5252 5725 6860 7541 7732 826 Size: 473 1135 681 191 1471 Fragments arranged by size: 2120 1471 1135 978 726 681 473 271 219 191 81 20 11 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8377 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8377 SmaI CCC'GGG Cuts at: 1872 4031 5704 1872 Size: 2159 1673 4545 Fragments arranged by size: 4545 2159 1673 SmlI C'TyrA_G Cuts at: 820 1017 1943 2703 2799 2822 3158 3359 3906 Size: 197 926 760 96 23 336 201 547 Cuts at: 3906 4869 5486 5645 6481 7382 7644 820 Size: 963 617 159 836 901 262 1553 Fragments arranged by size: 1553 963 926 901 836 760 617 547 336 262 201 197 159 96 23 SnaBI TAC'GTA Cuts at: 493 6168 493 Size: 5675 2702 SpeI A'CTAG_T Cuts at: 152 3584 152 Size: 3432 4945 SphI G_CATG'C Cuts at: 1132 2342 5735 1132 Size: 1210 3393 3774 Fragments arranged by size: 3774 3393 1210 SspI AAT'ATT Cuts at: 5 52 6301 5 Size: 47 6249 2081 Fragments arranged by size: 6249 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8377 StyI C'CwwG_G Cuts at: 513 1065 3915 4550 4592 5655 5816 513 Size: 552 2850 635 42 1063 161 3074 Fragments arranged by size: 3074 2850 1063 635 552 161 42 SwaI ATTT'AAAT Cuts at: 4200 4200 Size: 8377 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2831 4246 4440 6404 6421 6574 6759 Size: 1255 1251 1415 194 1964 17 153 185 Cuts at: 6759 7204 325 Size: 445 1498 Fragments arranged by size: 1964 1498 1415 1255 1251 445 194 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4311 5003 6623 Size: 274 80 33 51 1443 2334 692 1620 Cuts at: 6623 8335 96 Size: 1712 138 Fragments arranged by size: 2334 1712 1620 1443 692 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2348 2380 2854 2886 4314 4346 4805 4834 4837 Size: 32 474 32 1428 32 459 29 3 Cuts at: 4837 4866 8337 8369 2348 Size: 29 3471 32 2356 Fragments arranged by size: 3471 2356 1428 474 459 32 32 32 32 29 29 3 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8377 XbaI T'CTAG_A Cuts at: 5713 5713 Size: 8377 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3512 4595 5158 5188 3512 Size: 1083 563 30 6701 Fragments arranged by size: 6701 1083 563 30 XhoI C'TCGA_G Cuts at: 1943 2799 3906 4869 1943 Size: 856 1107 963 5451 Fragments arranged by size: 5451 1107 963 856 XmaI C'CCGG_G Cuts at: 1870 4029 5702 1870 Size: 2159 1673 4545 Fragments arranged by size: 4545 2159 1673 XmnI GAAnn'nnTTC Cuts at: 4574 6506 4574 Size: 1932 6445 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6185 276 Size: 53 83 186 936 4651 2468 Fragments arranged by size: 4651 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BbeI BbvCI BfrBI Bpu10I BsiWI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI