Restriction map for
pFN21AA1336
(Circular) (Six-base) MAPSORT of: pFN21AA1336.seq Check: 5123 from: 1 to: 8248
pFN21AA1336 8248 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6058 278
Size: 53 83 186 936 4522 2468
Fragments arranged by size:
4522 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2473 3595 5045 5591 1937
Size: 536 1122 1450 546 4594
Fragments arranged by size:
4594 1450 1122 546 536
Acc65I G'GTAC_C
Cuts at: 2640 5569 2640
Size: 2929 5319
AclI AA'CG_TT
Cuts at: 1563 3230 6375 6748 1563
Size: 1667 3145 373 3063
Fragments arranged by size:
3145 3063 1667 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2486 4109 4134 4260 5171 6311 7695 7963
Size: 854 1623 25 126 911 1140 1384 268
Cuts at: 7963 1632
Size: 1917
Fragments arranged by size:
1917 1623 1384 1140 911 854 268 126
25
AfeI AGC'GCT
Cuts at: 4202 4202
Size: 8248
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8051
AflIII A'CryG_T
Cuts at: 1203 4900 7147 8124 1203
Size: 3697 2247 977 1327
Fragments arranged by size:
3697 2247 1327 977
AgeI A'CCGG_T
Cuts at: 7079 7079
Size: 8248
AhdI GACnn_n'nnGTC
Cuts at: 6977 6977
Size: 8248
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8248
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8248
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2069 2101 2069
Size: 32 8216
AlwNI CAG_nnn'CTG
Cuts at: 4132 4363 4634 5520 5991 7563 4132
Size: 231 271 886 471 1572 4817
Fragments arranged by size:
4817 1572 886 471 271 231
ApaLI G'TGCA_C
Cuts at: 6305 7461 6305
Size: 1156 7092
ApoI r'AATT_y
Cuts at: 1493 2028 2566 2631 3438 5557 5783 5935 8044
Size: 535 538 65 807 2119 226 152 2109
Cuts at: 8044 1493
Size: 1697
Fragments arranged by size:
2119 2109 1697 807 538 535 226 152
65
AseI AT'TA_AT
Cuts at: 160 1032 3399 6802 160
Size: 872 2367 3403 1606
Fragments arranged by size:
3403 2367 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8248
AvaI C'yCGr_G
Cuts at: 1870 1943 3726 4289 5573 1870
Size: 73 1783 563 1284 4545
Fragments arranged by size:
4545 1783 1284 563 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4665 4698 4665
Size: 33 8215
BamHI G'GATC_C
Cuts at: 3271 3517 3874 3964 5578 5945 3271
Size: 246 357 90 1614 367 5574
Fragments arranged by size:
5574 1614 367 357 246 90
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2640 3575 4101 5569 7024
Size: 325 217 627 853 935 526 1468 1455
Cuts at: 7024 618
Size: 1842
Fragments arranged by size:
1842 1468 1455 935 853 627 526 325
217
BanII G_rGCy'C
Cuts at: 1391 5567 1391
Size: 4176 4072
BbeI G_GCGC'C
Cuts at: 4105 4105
Size: 8248
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3073 3851 5159 5187 5328 928
Size: 971 1174 778 1308 28 141 3848
Fragments arranged by size:
3848 1308 1174 971 778 141 28
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6439 6473 1903
Size: 34 4502 34 3678
Fragments arranged by size:
4502 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2074 3082 6140 7350 2074
Size: 1008 3058 1210 2972
Fragments arranged by size:
3058 2972 1210 1008
BclI T'GATC_A
Cuts at: 1548 2331 1548
Size: 783 7465
BfrBI ATG'CAT
Cuts at: 3044 4061 4766 5072 3044
Size: 1017 705 306 6220
Fragments arranged by size:
6220 1017 705 306
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6859 136
Size: 107 122 71 631 605 5187 1525
Fragments arranged by size:
5187 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2391 5202 8243 2391
Size: 2811 3041 2396
Fragments arranged by size:
3041 2811 2396
BlpI GC'TnA_GC
Cuts at: 4498 5665 4498
Size: 1167 7081
Bme1580I G_kGCm'C
Cuts at: 1790 6309 7465 1790
Size: 4519 1156 2573
Fragments arranged by size:
4519 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8248
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2172 5979 6937
Size: 416 53 83 399 24 755 3807 958
Cuts at: 6937 442
Size: 1753
Fragments arranged by size:
3807 1753 958 755 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 5219 1056
Size: 4163 4085
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5224 5224
Size: 8248
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2631 4340 6908 1239
Size: 1392 1709 2568 2579
Fragments arranged by size:
2579 2568 1709 1392
Bpu10I CC'TnA_GC
Cuts at: 2018 5496 2018
Size: 3478 4770
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 6373 7238 7536 6373
Size: 865 298 7085
Fragments arranged by size:
7085 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3134 3420 4017 5282 6911 882
Size: 617 1635 286 597 1265 1629 2219
Fragments arranged by size:
2219 1635 1629 1265 617 597 286
BsaAI yAC'GTr
Cuts at: 493 1204 2662 2770 4901 6039 493
Size: 711 1458 108 2131 1138 2702
Fragments arranged by size:
2702 2131 1458 1138 711 108
BsaBI GATnn'nnATC
Cuts at: 2442 3186 8242 2442
Size: 744 5056 2448
Fragments arranged by size:
5056 2448 744
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4102 6055 6437 7072
Size: 53 83 186 936 2569 1953 382 635
Cuts at: 7072 275
Size: 1451
Fragments arranged by size:
2569 1953 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 6681 7079 7353 7500 7911 6681
Size: 398 274 147 411 7018
Fragments arranged by size:
7018 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4344 4374 1178
Size: 30 165 30 2941 30 5052
Fragments arranged by size:
5052 2941 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2339 3215 1186
Size: 1153 876 6219
Fragments arranged by size:
6219 1153 876
BseYI C'CCAG_C
Cuts at: 4968 7451 7947 4968
Size: 2483 496 5269
Fragments arranged by size:
5269 2483 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2882 2910 5130 5488 1163
Size: 573 1146 28 2220 358 3923
Fragments arranged by size:
3923 2220 1146 573 358 28
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5698 6459 7487 1540
Size: 275 186 3697 761 1028 2301
Fragments arranged by size:
3697 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4033 6309 6394 7465 4033
Size: 2276 85 1071 4816
Fragments arranged by size:
4816 2276 1071 85
BsmI GAATG_Cn'
Cuts at: 2380 4462 4969 5759 5852 2380
Size: 2082 507 790 93 4776
Fragments arranged by size:
4776 2082 790 507 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2129 2129
Size: 8248
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 4033 5567 6309 6394 7465 1391
Size: 399 2243 1534 742 85 1071 2174
Fragments arranged by size:
2243 2174 1534 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 7911 7911
Size: 8248
BspHI T'CATG_A
Cuts at: 6137 7867 6137
Size: 1730 6518
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4251 4685 5603 844
Size: 3407 434 918 3489
Fragments arranged by size:
3489 3407 918 434
BsrBI CCG'CTC
Cuts at: 1394 1823 6135 1394
Size: 429 4312 3507
Fragments arranged by size:
4312 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2622 3315 3840 6743 6917 65
Size: 1183 1374 693 525 2903 174 1396
Fragments arranged by size:
2903 1396 1374 1183 693 525 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6892 7079 1700
Size: 110 5082 187 2869
Fragments arranged by size:
5082 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 4462 4474 96
Size: 4366 12 3870
Fragments arranged by size:
4366 3870 12
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8248
BssSI C'ACGA_G
Cuts at: 6308 7320 6308
Size: 1012 7236
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4634 5475 794
Size: 3840 841 3567
Fragments arranged by size:
3840 3567 841
BstBI TT'CG_AA
Cuts at: 7052 7052
Size: 8248
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8248
BstYI r'GATC_y
Cuts at: 1972 2391 3271 3517 3874 3964 4135 4306 4373
Size: 419 880 246 357 90 171 171 67
Cuts at: 4373 4745 5202 5578 5945 6330 6347 7799 7885
Size: 372 457 376 367 385 17 1452 86
Cuts at: 7885 7897 8243 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 880 457 419 385 376 372
367 357 346 246 171 171 90 86
67 17 12
BstZ17I GTA'TAC
Cuts at: 2474 2474
Size: 8248
Bsu36I CC'TnA_GG
Cuts at: 3195 3879 3195
Size: 684 7564
BtgI C'CryG_G
Cuts at: 513 1065 2644 513
Size: 552 1579 6117
Fragments arranged by size:
6117 1579 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7509
Fragments arranged by size:
7509 683 56
BtsI GCAGTG_nn'
Cuts at: 2063 4143 4635 5142 5476 5764 6558 6578 2063
Size: 2080 492 507 334 288 794 20 3733
Fragments arranged by size:
3733 2080 794 507 492 334 288 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8248
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8213
DraI TTT'AAA
Cuts at: 5552 5552
Size: 8248
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2510 3060 7255 809
Size: 507 1194 550 4195 1802
Fragments arranged by size:
4195 1802 1194 550 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 5695 6584 8213
Size: 54 1425 50 275 18 3865 889 1629
Cuts at: 8213 8
Size: 43
Fragments arranged by size:
3865 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 5695 1537
Size: 275 3883 4090
Fragments arranged by size:
4090 3883 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3778 4128 4263 4866 5166 6178 1353
Size: 2425 350 135 603 300 1012 3423
Fragments arranged by size:
3423 2425 1012 603 350 300 135
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6835 7209 7355 387
Size: 1064 138 5246 374 146 1280
Fragments arranged by size:
5246 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2486 2631 4109 4134 4260 4340 5171
Size: 393 854 145 1478 25 126 80 831
Cuts at: 5171 6311 6908 7695 7963 1239
Size: 1140 597 787 268 1524
Fragments arranged by size:
1524 1478 1140 854 831 787 597 393
268 145 126 80 25
EcoNI CCTnn'n_nnAGG
Cuts at: 5495 5495
Size: 8248
EcoO109I rG'GnC_Cy
Cuts at: 2164 2164
Size: 8248
EcoRI G'AATT_C
Cuts at: 2631 5557 5935 2631
Size: 2926 378 4944
Fragments arranged by size:
4944 2926 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2534 4139 4911 2534
Size: 1605 772 5871
Fragments arranged by size:
5871 1605 772
FspI TGC'GCA
Cuts at: 5227 6754 5227
Size: 1527 6721
HaeII r_GCGC'y
Cuts at: 1678 4105 4204 7395 1678
Size: 2427 99 3191 2531
Fragments arranged by size:
3191 2531 2427 99
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2323 2355 2376 2408 3547
Size: 32 135 32 751 32 21 32 1139
Cuts at: 3547 3577 3579 3609 3995 3997 4027 4029 5194
Size: 30 2 30 386 2 30 2 1165
Cuts at: 5194 5226 6889 6921 6963 6995 1373
Size: 32 1663 32 42 32 2626
Fragments arranged by size:
2626 1663 1165 1139 751 386 135 42
32 32 32 32 32 32 32 30
30 30 21 2 2 2
HincII GTy'rAC
Cuts at: 669 1657 1938 2262 4655 5592 5838 669
Size: 988 281 324 2393 937 246 3079
Fragments arranged by size:
3079 2393 988 937 324 281 246
HindIII A'AGCT_T
Cuts at: 748 5262 748
Size: 4514 3734
HpaI GTT'AAC
Cuts at: 5838 5838
Size: 8248
KasI G'GCGC_C
Cuts at: 4101 4101
Size: 8248
KpnI G_GTAC'C
Cuts at: 2644 5573 2644
Size: 2929 5319
MfeI C'AATT_G
Cuts at: 5847 5847
Size: 8248
MluI A'CGCG_T
Cuts at: 8124 8124
Size: 8248
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3739 4027 4591 7362 7546 1305
Size: 2434 288 564 2771 184 2007
Fragments arranged by size:
2771 2434 2007 564 288 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6426
Fragments arranged by size:
6426 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2730 2762 3450 6206 6565 6724 518
Size: 648 1564 32 688 2756 359 159 2042
Fragments arranged by size:
2756 2042 1564 688 648 359 159 32
MspA1I CmG'CkG
Cuts at: 1606 5664 6341 7489 7734 1606
Size: 4058 677 1148 245 2120
Fragments arranged by size:
4058 2120 1148 677 245
NarI GG'CG_CC
Cuts at: 4102 4102
Size: 8248
NcoI C'CATG_G
Cuts at: 513 1065 2644 513
Size: 552 1579 6117
Fragments arranged by size:
6117 1579 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8248
NheI G'CTAG_C
Cuts at: 1052 5215 1052
Size: 4163 4085
NotI GC'GGCC_GC
Cuts at: 5695 5695
Size: 8248
NsiI A_TGCA'T
Cuts at: 3046 4063 4768 5074 3046
Size: 1017 705 306 6220
Fragments arranged by size:
6220 1017 705 306
NspI r_CATG'y
Cuts at: 1132 1229 2097 2767 5232 5424 5606 7151 1132
Size: 97 868 670 2465 192 182 1545 2229
Fragments arranged by size:
2465 2229 1545 868 670 192 182 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8248
PciI A'CATG_T
Cuts at: 7147 7147
Size: 8248
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 8248
PfoI T'CCnGG_A
Cuts at: 2066 2908 4398 2066
Size: 842 1490 5916
Fragments arranged by size:
5916 1490 842
PmeI GTTT'AAAC
Cuts at: 5552 5552
Size: 8248
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6317 6349 7830 7862 6317
Size: 32 1481 32 6703
Fragments arranged by size:
6703 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8248
PsiI TTA'TAA
Cuts at: 3009 5818 3009
Size: 2809 5439
PspXI vC'TCGA_Gb
Cuts at: 3726 3726
Size: 8248
PstI C_TGCA'G
Cuts at: 830 2058 4150 5600 830
Size: 1228 2092 1450 3478
Fragments arranged by size:
3478 2092 1450 1228
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8248
SalI G'TCGA_C
Cuts at: 1936 5590 1936
Size: 3654 4594
SapI GCTCTTCn'nnn_
Cuts at: 4128 5166 4128
Size: 1038 7210
SbfI CC_TGCA'GG
Cuts at: 5600 5600
Size: 8248
ScaI AGT'ACT
Cuts at: 4353 6496 8208 4353
Size: 2143 1712 4393
Fragments arranged by size:
4393 2143 1712
SfcI C'TryA_G
Cuts at: 826 1045 2054 3033 4146 5512 5596 6731 7412
Size: 219 1009 979 1113 1366 84 1135 681
Cuts at: 7412 7603 826
Size: 191 1471
Fragments arranged by size:
1471 1366 1135 1113 1009 979 681 219
191 84
SfoI GGC'GCC
Cuts at: 4103 4103
Size: 8248
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8248
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8248
SmaI CCC'GGG
Cuts at: 1872 5575 1872
Size: 3703 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3726 4289 6352 7253 7515 820
Size: 197 926 1783 563 2063 901 262 1553
Fragments arranged by size:
2063 1783 1553 926 901 563 262 197
SnaBI TAC'GTA
Cuts at: 493 2770 6039 493
Size: 2277 3269 2702
Fragments arranged by size:
3269 2702 2277
SpeI A'CTAG_T
Cuts at: 152 3238 152
Size: 3086 5162
SphI G_CATG'C
Cuts at: 1132 2097 5232 5606 1132
Size: 965 3135 374 3774
Fragments arranged by size:
3774 3135 965 374
SspI AAT'ATT
Cuts at: 5 52 3071 3623 4469 6172 5
Size: 47 3019 552 846 1703 2081
Fragments arranged by size:
3019 2081 1703 846 552 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8248
StyI C'CwwG_G
Cuts at: 513 1065 2644 3267 4180 5013 5687 513
Size: 552 1579 623 913 833 674 3074
Fragments arranged by size:
3074 1579 913 833 674 623 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3999 6275 6292 6445 6630 7075 325
Size: 1255 2419 2276 17 153 185 445 1498
Fragments arranged by size:
2419 2276 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3235 4351 4462
Size: 274 80 33 51 1443 1258 1116 111
Cuts at: 4462 4474 4918 6494 8206 96
Size: 12 444 1576 1712 138
Fragments arranged by size:
1712 1576 1443 1258 1116 444 274 138
111 80 51 33 12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3502 3534 3788 3820 5455 5487 8208 8240 3502
Size: 32 254 32 1635 32 2721 32 3510
Fragments arranged by size:
3510 2721 1635 254 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8248
XbaI T'CTAG_A
Cuts at: 5584 5584
Size: 8248
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5011 5011
Size: 8248
XhoI C'TCGA_G
Cuts at: 1943 3726 4289 1943
Size: 1783 563 5902
Fragments arranged by size:
5902 1783 563
XmaI C'CCGG_G
Cuts at: 1870 5573 1870
Size: 3703 4545
XmnI GAAnn'nnTTC
Cuts at: 3888 4993 5037 6377 3888
Size: 1105 44 1340 5759
Fragments arranged by size:
5759 1340 1105 44
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6056 276
Size: 53 83 186 936 4522 2468
Fragments arranged by size:
4522 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PspXI PstI PvuI SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BbvCI BsiWI BstXI DraIII
EcoICRI EcoRV FseI FspAI NaeI NgoMIV NruI PacI
PmlI PpuMI PspOMI PsrI PvuII RsrII SacI SacII
SanDI SexAI SfiI SrfI SwaI