(Circular) (Six-base) MAPSORT of: pFN21AA1319.seq Check: 102 from: 1 to: 8314 pFN21AA1319 8314 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6124 278 Size: 53 83 186 936 4588 2468 Fragments arranged by size: 4588 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2046 5222 5553 5657 1937 Size: 109 3176 331 104 4594 Fragments arranged by size: 4594 3176 331 109 104 Acc65I G'GTAC_C Cuts at: 5635 5635 Size: 8314 AclI AA'CG_TT Cuts at: 1563 6441 6814 1563 Size: 4878 373 3063 Fragments arranged by size: 4878 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2517 2810 3422 3524 3681 4262 4721 4916 Size: 885 293 612 102 157 581 459 195 Cuts at: 4916 5114 5214 6377 7761 8029 1632 Size: 198 100 1163 1384 268 1917 Fragments arranged by size: 1917 1384 1163 885 612 581 459 293 268 198 195 157 102 100 AflII C'TTAA_G Cuts at: 820 1017 3926 820 Size: 197 2909 5208 Fragments arranged by size: 5208 2909 197 AflIII A'CryG_T Cuts at: 1203 3364 3553 7213 8190 1203 Size: 2161 189 3660 977 1327 Fragments arranged by size: 3660 2161 1327 977 189 AgeI A'CCGG_T Cuts at: 7145 7145 Size: 8314 AhdI GACnn_n'nnGTC Cuts at: 4254 7043 4254 Size: 2789 5525 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8314 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2486 3236 3617 1184 Size: 1302 750 381 5881 Fragments arranged by size: 5881 1302 750 381 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4038 4070 5247 5279 4038 Size: 32 1177 32 7073 Fragments arranged by size: 7073 1177 32 32 AlwNI CAG_nnn'CTG Cuts at: 2435 2773 3229 4081 4240 4312 4333 4459 4756 Size: 338 456 852 159 72 21 126 297 Cuts at: 4756 4768 4831 4840 5050 5498 5610 6057 7629 Size: 12 63 9 210 448 112 447 1572 Cuts at: 7629 2435 Size: 3120 Fragments arranged by size: 3120 1572 852 456 448 447 338 297 210 159 126 112 72 63 21 12 9 ApaI G_GGCC'C Cuts at: 2042 3514 4471 4888 2042 Size: 1472 957 417 5468 Fragments arranged by size: 5468 1472 957 417 ApaLI G'TGCA_C Cuts at: 2853 6371 7527 2853 Size: 3518 1156 3640 Fragments arranged by size: 3640 3518 1156 ApoI r'AATT_y Cuts at: 1493 4205 5540 5623 5849 6001 8110 1493 Size: 2712 1335 83 226 152 2109 1697 Fragments arranged by size: 2712 2109 1697 1335 226 152 83 AseI AT'TA_AT Cuts at: 160 1032 6868 160 Size: 872 5836 1606 Fragments arranged by size: 5836 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8314 AvaI C'yCGr_G Cuts at: 1870 1943 2034 2583 3420 3855 4251 4904 5639 Size: 73 91 549 837 435 396 653 735 Cuts at: 5639 1870 Size: 4545 Fragments arranged by size: 4545 837 735 653 549 435 396 91 73 BamHI G'GATC_C Cuts at: 2446 5644 6011 2446 Size: 3198 367 4749 Fragments arranged by size: 4749 3198 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2014 5635 7090 618 Size: 325 217 627 227 3621 1455 1842 Fragments arranged by size: 3621 1842 1455 627 325 227 217 BanII G_rGCy'C Cuts at: 1391 2034 2042 2284 2291 3276 3514 4471 4888 Size: 643 8 242 7 985 238 957 417 Cuts at: 4888 5022 5531 5633 1391 Size: 134 509 102 4072 Fragments arranged by size: 4072 985 957 643 509 417 242 238 134 102 8 7 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2523 4718 5102 5213 5280 928 Size: 971 624 2195 384 111 67 3962 Fragments arranged by size: 3962 2195 971 624 384 111 67 BbvCI CC'TCA_GC Cuts at: 3473 5009 3473 Size: 1536 6778 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6505 6539 1903 Size: 34 4568 34 3678 Fragments arranged by size: 4568 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6206 7416 6206 Size: 1210 7104 BclI T'GATC_A Cuts at: 1548 1548 Size: 8314 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2426 4391 4703 Size: 107 122 71 631 605 754 1965 312 Cuts at: 4703 4772 6925 136 Size: 69 2153 1525 Fragments arranged by size: 2153 1965 1525 754 631 605 312 122 107 71 69 BglII A'GATC_T Cuts at: 8309 8309 Size: 8314 BlpI GC'TnA_GC Cuts at: 2028 4419 4518 4785 5312 5491 5525 5731 2028 Size: 2391 99 267 527 179 34 206 4611 Fragments arranged by size: 4611 2391 527 267 206 179 99 34 Bme1580I G_kGCm'C Cuts at: 1790 2042 2105 2744 2857 3514 4396 4471 4888 Size: 252 63 639 113 657 882 75 417 Cuts at: 4888 6375 7531 1790 Size: 1487 1156 2573 Fragments arranged by size: 2573 1487 1156 882 657 639 417 252 113 75 63 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8314 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2435 2827 6045 Size: 416 53 83 399 24 1018 392 3218 Cuts at: 6045 7003 442 Size: 958 1753 Fragments arranged by size: 3218 1753 1018 958 416 399 392 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2483 1056 Size: 1427 6887 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2998 3547 4732 5485 2998 Size: 549 1185 753 5827 Fragments arranged by size: 5827 1185 753 549 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2495 2874 3047 3200 3212 3254 3260 3347 Size: 1256 379 173 153 12 42 6 87 Cuts at: 3347 3653 3707 3888 4040 4058 4334 4355 4418 Size: 306 54 181 152 18 276 21 63 Cuts at: 4418 4493 4544 4604 4652 4740 4862 5072 5258 Size: 75 51 60 48 88 122 210 186 Cuts at: 5258 5423 5480 6974 1239 Size: 165 57 1494 2579 Fragments arranged by size: 2579 1494 1256 379 306 276 210 186 181 173 165 153 152 122 88 87 75 63 60 57 54 51 48 42 21 18 12 6 Bpu10I CC'TnA_GC Cuts at: 2133 3378 3387 3473 5009 2133 Size: 1245 9 86 1536 5438 Fragments arranged by size: 5438 1536 1245 86 9 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3878 5174 6439 7304 7602 3878 Size: 1296 1265 865 298 4590 Fragments arranged by size: 4590 1296 1265 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2898 3323 3410 4178 4907 6977 882 Size: 617 1399 425 87 768 729 2070 2219 Fragments arranged by size: 2219 2070 1399 768 729 617 425 87 BsaAI yAC'GTr Cuts at: 493 1204 6105 493 Size: 711 4901 2702 Fragments arranged by size: 4901 2702 711 BsaBI GATnn'nnATC Cuts at: 8308 8308 Size: 8314 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2125 6121 6503 7138 Size: 53 83 186 936 592 3996 382 635 Cuts at: 7138 275 Size: 1451 Fragments arranged by size: 3996 1451 936 635 592 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3909 6747 7145 7419 7566 7977 3909 Size: 2838 398 274 147 411 4246 Fragments arranged by size: 4246 2838 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2678 2708 3476 3506 4038 Size: 30 165 30 1275 30 768 30 532 Cuts at: 4038 4068 5051 5081 1178 Size: 30 983 30 4411 Fragments arranged by size: 4411 1275 983 768 532 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2384 2852 2863 3019 3125 3209 3253 3479 Size: 1198 468 11 156 106 84 44 226 Cuts at: 3479 3536 3704 3716 3809 3821 3935 4352 4379 Size: 57 168 12 93 12 114 417 27 Cuts at: 4379 4427 4490 4541 4958 5426 1186 Size: 48 63 51 417 468 4074 Fragments arranged by size: 4074 1198 468 468 417 417 226 168 156 114 106 93 84 63 57 51 48 44 27 12 12 11 BseYI C'CCAG_C Cuts at: 2128 2186 3255 3965 4144 4626 4674 4824 5397 Size: 58 1069 710 179 482 48 150 573 Cuts at: 5397 7517 8013 2128 Size: 2120 496 2429 Fragments arranged by size: 2429 2120 1069 710 573 496 482 179 150 58 48 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2112 2195 3232 1163 Size: 573 376 83 1037 6245 Fragments arranged by size: 6245 1037 573 376 83 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5764 6525 7553 1540 Size: 275 186 3763 761 1028 2301 Fragments arranged by size: 3763 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2208 2284 2291 2857 3276 3894 4503 5022 5531 Size: 76 7 566 419 618 609 519 509 Cuts at: 5531 6375 6460 7531 2208 Size: 844 85 1071 2991 Fragments arranged by size: 2991 1071 844 618 609 566 519 509 419 85 76 7 BsmI GAATG_Cn' Cuts at: 4354 5825 5918 4354 Size: 1471 93 6750 Fragments arranged by size: 6750 1471 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4163 4163 Size: 8314 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2034 2042 2105 2208 2284 2291 2744 Size: 399 244 8 63 103 76 7 453 Cuts at: 2744 2857 3276 3514 3894 4396 4471 4503 4888 Size: 113 419 238 380 502 75 32 385 Cuts at: 4888 5022 5531 5633 6375 6460 7531 1391 Size: 134 509 102 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 509 502 453 419 399 385 380 244 238 134 113 103 102 85 76 75 63 32 8 7 BspEI T'CCGG_A Cuts at: 3909 7977 3909 Size: 4068 4246 BspHI T'CATG_A Cuts at: 6203 7933 6203 Size: 1730 6584 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5669 844 Size: 4825 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3184 3487 4789 5182 5242 5281 5490 Size: 429 1361 303 1302 393 60 39 209 Cuts at: 5490 6201 1394 Size: 711 3507 Fragments arranged by size: 3507 1361 1302 711 429 393 303 209 60 39 BsrDI GCAATG_nn' Cuts at: 65 1248 5568 6809 6983 65 Size: 1183 4320 1241 174 1396 Fragments arranged by size: 4320 1396 1241 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4212 6958 7145 1700 Size: 110 2402 2746 187 2869 Fragments arranged by size: 2869 2746 2402 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8314 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8314 BssSI C'ACGA_G Cuts at: 6374 7386 6374 Size: 1012 7302 BstAPI GCAn_nnn'nTGC Cuts at: 794 3229 4609 4768 794 Size: 2435 1380 159 4340 Fragments arranged by size: 4340 2435 1380 159 BstBI TT'CG_AA Cuts at: 2747 7118 2747 Size: 4371 3943 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8314 BstYI r'GATC_y Cuts at: 1972 2446 3745 4746 5644 6011 6396 6413 7865 Size: 474 1299 1001 898 367 385 17 1452 Cuts at: 7865 7951 7963 8309 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1299 1001 898 474 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2275 2969 4010 5318 5384 2275 Size: 694 1041 1308 66 5205 Fragments arranged by size: 5205 1308 1041 694 66 BtgI C'CryG_G Cuts at: 513 1065 2004 2050 2663 2843 3075 4956 513 Size: 552 939 46 613 180 232 1881 3871 Fragments arranged by size: 3871 1881 939 613 552 232 180 46 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7575 Fragments arranged by size: 7575 683 56 BtsI GCAGTG_nn' Cuts at: 2517 2938 3633 4170 5576 5830 6624 6644 2517 Size: 421 695 537 1406 254 794 20 4187 Fragments arranged by size: 4187 1406 794 695 537 421 254 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8314 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8279 DraI TTT'AAA Cuts at: 5618 5618 Size: 8314 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2054 7321 809 Size: 507 738 5267 1802 Fragments arranged by size: 5267 1802 738 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2596 4122 4987 Size: 54 1425 50 275 18 766 1526 865 Cuts at: 4987 5106 5761 6650 8279 8 Size: 119 655 889 1629 43 Fragments arranged by size: 1629 1526 1425 889 865 766 655 275 119 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5761 1537 Size: 275 3949 4090 Fragments arranged by size: 4090 3949 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3129 3201 3429 3798 4092 4356 5190 5352 Size: 1776 72 228 369 294 264 834 162 Cuts at: 5352 6244 1353 Size: 892 3423 Fragments arranged by size: 3423 1776 892 834 369 294 264 228 162 72 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4199 6901 7275 7421 387 Size: 1064 138 2610 2702 374 146 1280 Fragments arranged by size: 2702 2610 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2282 2289 3274 5020 5529 2282 Size: 7 985 1746 509 5067 Fragments arranged by size: 5067 1746 985 509 7 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2495 2517 2810 2874 3047 3200 3212 Size: 393 863 22 293 64 173 153 12 Cuts at: 3212 3254 3260 3347 3422 3524 3653 3681 3707 Size: 42 6 87 75 102 129 28 26 Cuts at: 3707 3888 4040 4058 4262 4334 4355 4418 4493 Size: 181 152 18 204 72 21 63 75 Cuts at: 4493 4544 4604 4652 4721 4740 4862 4916 5072 Size: 51 60 48 69 19 122 54 156 Cuts at: 5072 5114 5214 5258 5423 5480 6377 6974 7761 Size: 42 100 44 165 57 897 597 787 Cuts at: 7761 8029 1239 Size: 268 1524 Fragments arranged by size: 1524 897 863 787 597 393 293 268 204 181 173 165 156 153 152 129 122 102 100 87 75 75 72 69 64 63 60 57 54 51 48 44 42 42 28 26 22 21 19 18 12 6 EcoNI CCTnn'n_nnAGG Cuts at: 2972 3001 3518 4412 4442 4475 2972 Size: 29 517 894 30 33 6811 Fragments arranged by size: 6811 894 517 33 30 29 EcoO109I rG'GnC_Cy Cuts at: 2038 2039 2271 2379 2432 2821 2860 3374 3406 Size: 1 232 108 53 389 39 514 32 Cuts at: 3406 3510 3511 4342 4468 4765 4884 5047 5089 Size: 104 1 831 126 297 119 163 42 Cuts at: 5089 2038 Size: 5263 Fragments arranged by size: 5263 831 514 389 297 232 163 126 119 108 104 53 42 39 32 1 1 EcoRI G'AATT_C Cuts at: 4205 5540 5623 6001 4205 Size: 1335 83 378 6518 Fragments arranged by size: 6518 1335 378 83 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3310 3827 3310 Size: 517 7797 FspI TGC'GCA Cuts at: 6820 6820 Size: 8314 HaeII r_GCGC'y Cuts at: 1678 2692 3292 4681 7461 1678 Size: 1014 600 1389 2780 2531 Fragments arranged by size: 2780 2531 1389 1014 600 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2089 2121 2678 2710 3025 Size: 32 135 32 517 32 557 32 315 Cuts at: 3025 3057 4240 4272 5051 5083 5440 5472 5549 Size: 32 1183 32 779 32 357 32 77 Cuts at: 5549 5581 6955 6987 7029 7061 1373 Size: 32 1374 32 42 32 2626 Fragments arranged by size: 2626 1374 1183 779 557 517 357 315 135 77 42 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2567 3495 5658 5904 669 Size: 988 281 629 928 2163 246 3079 Fragments arranged by size: 3079 2163 988 928 629 281 246 HindIII A'AGCT_T Cuts at: 748 5481 748 Size: 4733 3581 HpaI GTT'AAC Cuts at: 5904 5904 Size: 8314 KpnI G_GTAC'C Cuts at: 5639 5639 Size: 8314 MfeI C'AATT_G Cuts at: 5913 5913 Size: 8314 MluI A'CGCG_T Cuts at: 8190 8190 Size: 8314 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2570 3969 4557 7428 7612 1305 Size: 1265 1399 588 2871 184 2007 Fragments arranged by size: 2871 2007 1399 1265 588 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 5108 10 Size: 54 1425 343 3276 3216 Fragments arranged by size: 3276 3216 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 5292 6272 6631 6790 518 Size: 648 4126 980 359 159 2042 Fragments arranged by size: 4126 2042 980 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2845 3190 3472 3778 4000 4153 4240 4309 Size: 1239 345 282 306 222 153 87 69 Cuts at: 4309 4333 4423 4492 4630 4894 5008 5495 5730 Size: 24 90 69 138 264 114 487 235 Cuts at: 5730 6407 7555 7800 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1239 1148 677 487 345 306 282 264 245 235 222 153 138 114 90 87 69 69 24 NaeI GCC'GGC Cuts at: 4214 4214 Size: 8314 NcoI C'CATG_G Cuts at: 513 1065 2004 2050 513 Size: 552 939 46 6777 Fragments arranged by size: 6777 939 552 46 NdeI CA'TA_TG Cuts at: 387 2943 387 Size: 2556 5758 NgoMIV G'CCGG_C Cuts at: 4212 4212 Size: 8314 NheI G'CTAG_C Cuts at: 1052 2479 1052 Size: 1427 6887 NotI GC'GGCC_GC Cuts at: 5761 5761 Size: 8314 NspI r_CATG'y Cuts at: 1132 1229 2473 3368 3557 3604 5291 5672 7217 Size: 97 1244 895 189 47 1687 381 1545 Cuts at: 7217 1132 Size: 2229 Fragments arranged by size: 2229 1687 1545 1244 895 381 189 97 47 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8314 PciI A'CATG_T Cuts at: 3364 3553 7213 3364 Size: 189 3660 4465 Fragments arranged by size: 4465 3660 189 PflMI CCAn_nnn'nTGG Cuts at: 1108 2087 2706 3340 3547 3961 4692 1108 Size: 979 619 634 207 414 731 4730 Fragments arranged by size: 4730 979 731 634 619 414 207 PmeI GTTT'AAAC Cuts at: 5618 5618 Size: 8314 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2303 2335 4038 4070 6383 6415 7896 7928 2303 Size: 32 1703 32 2313 32 1481 32 2689 Fragments arranged by size: 2689 2313 1703 1481 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2821 2860 3374 3406 5047 5089 2821 Size: 39 514 32 1641 42 6046 Fragments arranged by size: 6046 1641 514 42 39 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8314 PsiI TTA'TAA Cuts at: 4641 5884 4641 Size: 1243 7071 PspOMI G'GGCC_C Cuts at: 2038 3510 4467 4884 2038 Size: 1472 957 417 5468 Fragments arranged by size: 5468 1472 957 417 PspXI vC'TCGA_Gb Cuts at: 4251 4251 Size: 8314 PstI C_TGCA'G Cuts at: 830 2877 3225 3351 3678 3777 3939 4308 4614 Size: 2047 348 126 327 99 162 369 306 Cuts at: 4614 4773 4836 5220 5666 830 Size: 159 63 384 446 3478 Fragments arranged by size: 3478 2047 446 384 369 348 327 306 162 159 126 99 63 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8314 PvuII CAG'CTG Cuts at: 3190 3472 3778 4000 4240 4333 4630 4894 5008 Size: 282 306 222 240 93 297 264 114 Cuts at: 5008 5495 3190 Size: 487 6009 Fragments arranged by size: 6009 487 306 297 282 264 240 222 114 93 SacI G_AGCT'C Cuts at: 2284 2291 3276 5022 5531 2284 Size: 7 985 1746 509 5067 Fragments arranged by size: 5067 1746 985 509 7 SacII CC_GC'GG Cuts at: 2846 2846 Size: 8314 SalI G'TCGA_C Cuts at: 1936 5656 1936 Size: 3720 4594 SbfI CC_TGCA'GG Cuts at: 4773 5666 4773 Size: 893 7421 ScaI AGT'ACT Cuts at: 6562 8274 6562 Size: 1712 6602 SexAI A'CCwGG_T Cuts at: 4995 4995 Size: 8314 SfcI C'TryA_G Cuts at: 826 1045 2873 3116 3221 3347 3674 3773 3935 Size: 219 1828 243 105 126 327 99 162 Cuts at: 3935 4304 4610 4769 4832 5186 5216 5597 5662 Size: 369 306 159 63 354 30 381 65 Cuts at: 5662 6797 7478 7669 826 Size: 1135 681 191 1471 Fragments arranged by size: 1828 1471 1135 681 381 369 354 327 306 243 219 191 162 159 126 105 99 65 63 30 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8314 SgrAI Cr'CCGG_yG Cuts at: 1700 4212 1700 Size: 2512 5802 SmaI CCC'GGG Cuts at: 1872 2036 2585 3422 5641 1872 Size: 164 549 837 2219 4545 Fragments arranged by size: 4545 2219 837 549 164 SmlI C'TyrA_G Cuts at: 820 1017 1943 3893 3926 4251 4904 5153 6418 Size: 197 926 1950 33 325 653 249 1265 Cuts at: 6418 7319 7581 820 Size: 901 262 1553 Fragments arranged by size: 1950 1553 1265 926 901 653 325 262 249 197 33 SnaBI TAC'GTA Cuts at: 493 6105 493 Size: 5612 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8314 SphI G_CATG'C Cuts at: 1132 2473 3604 5672 1132 Size: 1341 1131 2068 3774 Fragments arranged by size: 3774 2068 1341 1131 SspI AAT'ATT Cuts at: 5 52 6238 5 Size: 47 6186 2081 Fragments arranged by size: 6186 2081 47 StuI AGG'CCT Cuts at: 1517 2420 1517 Size: 903 7411 StyI C'CwwG_G Cuts at: 513 1065 2004 2050 2266 4107 4686 5070 5753 Size: 552 939 46 216 1841 579 384 683 Cuts at: 5753 513 Size: 3074 Fragments arranged by size: 3074 1841 939 683 579 552 384 216 46 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2032 4575 6341 6358 6511 6696 7141 Size: 1255 452 2543 1766 17 153 185 445 Cuts at: 7141 325 Size: 1498 Fragments arranged by size: 2543 1766 1498 1255 452 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 6560 8272 96 Size: 274 80 33 51 1443 4583 1712 138 Fragments arranged by size: 4583 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5039 5071 8274 8306 5039 Size: 32 3203 32 5047 Fragments arranged by size: 5047 3203 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2819 5549 1316 Size: 1503 2730 4081 Fragments arranged by size: 4081 2730 1503 XbaI T'CTAG_A Cuts at: 5650 5650 Size: 8314 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3962 4114 4393 5233 3962 Size: 152 279 840 7043 Fragments arranged by size: 7043 840 279 152 XhoI C'TCGA_G Cuts at: 1943 4251 4904 1943 Size: 2308 653 5353 Fragments arranged by size: 5353 2308 653 XmaI C'CCGG_G Cuts at: 1870 2034 2583 3420 5639 1870 Size: 164 549 837 2219 4545 Fragments arranged by size: 4545 2219 837 549 164 XmnI GAAnn'nnTTC Cuts at: 6443 6443 Size: 8314 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6122 276 Size: 53 83 186 936 4588 2468 Fragments arranged by size: 4588 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BfrBI BsiWI BstXI BstZ17I DraIII EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PsrI RsrII SanDI SapI SfiI SfoI SrfI SwaI