Restriction map for
pFN21AA1221
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# NO CUT ENZYMES ( 118 enzymes) #
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AatII AccII AccIII AclI AgeI
AhdI Aor51HI ApaI AscI Asp700I
AspEI AspI AvaI AviII AvrII
BalI BanII BbeI BbrPI BcgI
BciVI BcnI BlnI BlpI Bpu1102I
BsaAI BsaI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BsoBI BspDI
BspEI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I CelII CfoI Cfr10I ClaI
CpoI DraIII EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRV
FauI FseI FspI HaeII HapII
HhaI HinP1I HpaI HpaII KasI
KspI MluI MluNI MroI MscI
MspI MvnI NaeI NarI NciI
NcoI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI Psp1406I PvuI
RsrII SacI SacII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI SwaI Tth111I XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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Acc65I 1510 GGTACC G^GTACC
AcyI 2377 GACGCC G[GA]^CG[CT]C
AflII 258 CTTAAG C^TTAAG
Alw44I 1478 GTGCAC G^TGCAC
AlwNI 3413 CAGTGTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 734 CCTCAGG CC^T[ACGT]AGG
ApaLI 1478 GTGCAC G^TGCAC
AseI 2644 ATTAAT AT^TAAT
AsnI 2644 ATTAAT AT^TAAT
Asp718I 1510 GGTACC G^GTACC
BamHI 777 GGATCC G^GATCC
BanI 1510 GGTACC G^G[CT][GA]CC
BbvCI 2806 CCTCAGC CCTCAGC(-5/-2)
BclI 2246 TGATCA T^GATCA
BfrI 258 CTTAAG C^TTAAG
BglI 1625 GCCATCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 3721 AGATCT A^GATCT
BsaHI 2377 GACGCC G[GA]^CG[CT]C
BsiYI 3880 CCACGGACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 3880 CCACGGACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 1330 GTCCC GGGAC(10/14)
Bsp1407I 1234 TGTACA T^GTACA
BspHI 1712 TCATGA T^CATGA
BspLU11I 2090 ACATGT A^CATGT
BsrBI 159 GAGCGG CCGCTC(-3/-3)
BsrGI 1234 TGTACA T^GTACA
Bsu36I 734 CCTCAGG CC^T[ACGT]AGG
DraII 3813 AGGACCC [GA]G^G[ACGT]CC[CT]
DrdI 2865 GACTGATTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 3880 CCACGG C^C[GA][CT]GG
Eco81I 734 CCTCAGG CC^T[ACGT]AGG
EcoO109I 3813 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoT22I 1019 ATGCAT ATGCA^T
FbaI 2246 TGATCA T^GATCA
HgaI 2377 GACGC GACGC(5/10)
Hin1I 2377 GACGCC G[GA]^CG[CT]C
HindIII 2394 AAGCTT A^AGCTT
KpnI 1510 GGTACC GGTAC^C
MfeI 1515 CAATTG C^AATTG
MunI 1515 CAATTG C^AATTG
NsiI 1019 ATGCAT ATGCA^T
PflMI 3880 CCACGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 3813 AGGACCC [GA]G^G[AT]CC[CT]
PshBI 2644 ATTAAT AT^TAAT
PstI 2822 CTGCAG CTGCA^G
RcaI 1712 TCATGA T^CATGA
ScaI 1817 AGTACT AGT^ACT
SspBI 1234 TGTACA T^GTACA
StuI 2386 AGGCCT AGG^CCT
Van91I 3880 CCACGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 25 enzymes) #
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AciI 161 GCGG CCGC(-3/-1)
2037 GCGG
AflIII 2090 ACATGT A^C[GA][CT]GT
2412 ACGTGT
AspHI 296 GTGCTC G[AT]GC[AT]^C
1478 GTGCAC
AvaII 352 GGACC G^G[AT]CC
3814 GGACC
BmyI 296 GTGCTC G[AGT]GC[ACT]^C
1478 GTGCAC
BpmI 2705 CTGGAG CTGGAG(16/14)
3887 CTGGAG
BsaBI 2479 GATAGCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2641 GATATTAATC
BsiHKAI 296 GTGCTC G[AT]GC[AT]^C
1478 GTGCAC
BsmI 1621 GAATGC GAATGC(1/-1)
1824 GCATTC
Bsp1286I 296 GTGCTC G[AGT]GC[ACT]^C
1478 GTGCAC
BspMI 1194 ACCTGC ACCTGC(4/8)
3788 GCAGGT
BstXI 68 CCAACAATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2733 CCAAAACGATGG
Cfr13I 352 GGACC G^G[ACGT]CC
3814 GGACC
DraI 1800 TTTAAA TTT^AAA
1968 TTTAAA
EaeI 2038 CGGCCA [CT]^GGCC[GA]
3965 TGGCCG
EcoRI 615 GAATTC G^AATTC
3552 GAATTC
EcoT14I 1333 CCAAGG C^C[AT][AT]GG
2195 CCAAGG
MamI 2479 GATAGCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2641 GATATTAATC
MspA1I 473 CAGCTG C[AC]G^C[GT]G
484 CAGCTG
PshAI 2508 GACTGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3815 GACCCTTGTC
PvuII 473 CAGCTG CAG^CTG
484 CAGCTG
SapI 988 GAAGAGC GCTCTTC(1/4)
1213 GAAGAGC
Sau96I 352 GGACC G^G[ACGT]CC
3814 GGACC
SmlI 258 CTTAAG C^T[CT][GA]AG
2878 CTTGAG
StyI 1333 CCAAGG C^C[AT][AT]GG
2195 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AccI AcsI AfaI AluI AlwI
ApoI BbsI BbvI BfaI BpuAI
BsaJI BseRI BsmAI BsrDI BsrI
BssKI BstF5I BstNI BstYI Cac8I
DdeI DpnII EarI EcoRII Fnu4HI
FokI HaeIII HincII HindII HinfI
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MvaI MwoI NdeI
NdeII NlaIII NlaIV NspI PleI
RsaI Sau3AI ScrFI SfaNI SfcI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XbaI XhoII