(Circular) (Six-base) MAPSORT of: pFN21AA1221.seq Check: 1170 from: 1 to: 8686 pFN21AA1221 8686 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3208 3208 Size: 8686 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6496 278 Size: 53 83 186 936 4960 2468 Fragments arranged by size: 4960 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2495 2973 3009 6029 1937 Size: 558 478 36 3020 4594 Fragments arranged by size: 4594 3020 558 478 36 Acc65I G'GTAC_C Cuts at: 3515 6007 3515 Size: 2492 6194 AclI AA'CG_TT Cuts at: 1563 6813 7186 1563 Size: 5250 373 3063 Fragments arranged by size: 5250 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2250 2471 2583 3449 3774 5427 5967 6749 Size: 618 221 112 866 325 1653 540 782 Cuts at: 6749 8133 8401 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1653 1384 866 782 618 540 325 268 221 112 AflII C'TTAA_G Cuts at: 820 1017 2263 820 Size: 197 1246 7243 Fragments arranged by size: 7243 1246 197 AflIII A'CryG_T Cuts at: 1203 4095 4417 7585 8562 1203 Size: 2892 322 3168 977 1327 Fragments arranged by size: 3168 2892 1327 977 322 AgeI A'CCGG_T Cuts at: 7517 7517 Size: 8686 AhdI GACnn_n'nnGTC Cuts at: 7415 7415 Size: 8686 AleI CACnn'nnGTG Cuts at: 1166 4416 1166 Size: 3250 5436 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8686 AlwNI CAG_nnn'CTG Cuts at: 5423 6429 8001 5423 Size: 1006 1572 6108 Fragments arranged by size: 6108 1572 1006 ApaLI G'TGCA_C Cuts at: 3483 6743 7899 3483 Size: 3260 1156 4270 Fragments arranged by size: 4270 3260 1156 ApoI r'AATT_y Cuts at: 1493 2620 2733 3256 3444 4032 4312 4666 5446 Size: 1127 113 523 188 588 280 354 780 Cuts at: 5446 5557 5995 6221 6373 8482 1493 Size: 111 438 226 152 2109 1697 Fragments arranged by size: 2109 1697 1127 780 588 523 438 354 280 226 188 152 113 111 AseI AT'TA_AT Cuts at: 160 1032 4650 7240 160 Size: 872 3618 2590 1606 Fragments arranged by size: 3618 2590 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8686 AvaI C'yCGr_G Cuts at: 1870 1943 6011 1870 Size: 73 4068 4545 Fragments arranged by size: 4545 4068 73 BamHI G'GATC_C Cuts at: 2782 6016 6383 2782 Size: 3234 367 5085 Fragments arranged by size: 5085 3234 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3515 6007 7462 618 Size: 325 217 627 1728 2492 1455 1842 Fragments arranged by size: 2492 1842 1728 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 6005 1391 Size: 4614 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3066 3430 4482 5844 928 Size: 971 1167 364 1052 1362 3770 Fragments arranged by size: 3770 1362 1167 1052 971 364 BbvCI CC'TCA_GC Cuts at: 4812 4812 Size: 8686 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6877 6911 1903 Size: 34 4940 34 3678 Fragments arranged by size: 4940 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6578 7788 6578 Size: 1210 7476 BclI T'GATC_A Cuts at: 1548 4251 1548 Size: 2703 5983 BfrBI ATG'CAT Cuts at: 3026 3026 Size: 8686 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3636 7297 136 Size: 107 122 71 631 605 1964 3661 1525 Fragments arranged by size: 3661 1964 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5726 8681 5726 Size: 2955 5731 BlpI GC'TnA_GC Cuts at: 6103 6103 Size: 8686 Bme1580I G_kGCm'C Cuts at: 1790 3487 6747 7903 1790 Size: 1697 3260 1156 2573 Fragments arranged by size: 3260 2573 1697 1156 BmgBI CAC'GTC Cuts at: 1318 4418 5916 1318 Size: 3100 1498 4088 Fragments arranged by size: 4088 3100 1498 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2788 5481 6417 Size: 416 53 83 399 24 1371 2693 936 Cuts at: 6417 7375 442 Size: 958 1753 Fragments arranged by size: 2693 1753 1371 958 936 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8686 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4731 5913 7346 1239 Size: 3492 1182 1433 2579 Fragments arranged by size: 3492 2579 1433 1182 Bpu10I CC'TnA_GC Cuts at: 4812 4812 Size: 8686 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4904 6811 7676 7974 4904 Size: 1907 865 298 5616 Fragments arranged by size: 5616 1907 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 7349 882 Size: 617 5850 2219 Fragments arranged by size: 5850 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 6477 493 Size: 711 5273 2702 Fragments arranged by size: 5273 2702 711 BsaBI GATnn'nnATC Cuts at: 4488 4650 8680 4488 Size: 162 4030 4494 Fragments arranged by size: 4494 4030 162 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4383 6493 6875 7510 Size: 53 83 186 936 2850 2110 382 635 Cuts at: 7510 275 Size: 1451 Fragments arranged by size: 2850 2110 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 7119 7517 7791 7938 8349 7119 Size: 398 274 147 411 7456 Fragments arranged by size: 7456 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2334 2364 1178 Size: 30 165 30 931 30 7500 Fragments arranged by size: 7500 931 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2362 4107 5562 1186 Size: 1176 1745 1455 4310 Fragments arranged by size: 4310 1745 1455 1176 BseYI C'CCAG_C Cuts at: 4909 7889 8385 4909 Size: 2980 496 5210 Fragments arranged by size: 5210 2980 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 8113 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6136 6897 7925 1540 Size: 275 186 4135 761 1028 2301 Fragments arranged by size: 4135 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2305 3487 6747 6832 7903 2305 Size: 1182 3260 85 1071 3088 Fragments arranged by size: 3260 3088 1182 1071 85 BsmI GAATG_Cn' Cuts at: 3632 3829 6197 6290 3632 Size: 197 2368 93 6028 Fragments arranged by size: 6028 2368 197 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2305 3487 6005 6747 6832 7903 1391 Size: 399 515 1182 2518 742 85 1071 2174 Fragments arranged by size: 2518 2174 1182 1071 742 515 399 85 BspEI T'CCGG_A Cuts at: 8349 8349 Size: 8686 BspHI T'CATG_A Cuts at: 3717 6575 8305 3717 Size: 2858 1730 4098 Fragments arranged by size: 4098 2858 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3208 5784 6041 844 Size: 2364 2576 257 3489 Fragments arranged by size: 3489 2576 2364 257 BsrBI CCG'CTC Cuts at: 1394 1823 2166 6573 1394 Size: 429 343 4407 3507 Fragments arranged by size: 4407 3507 429 343 BsrDI GCAATG_nn' Cuts at: 65 1248 3256 3639 4074 7181 7355 65 Size: 1183 2008 383 435 3107 174 1396 Fragments arranged by size: 3107 2008 1396 1183 435 383 174 BsrFI r'CCGG_y Cuts at: 1700 1810 7330 7517 1700 Size: 110 5520 187 2869 Fragments arranged by size: 5520 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3239 96 Size: 3143 5543 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8686 BssSI C'ACGA_G Cuts at: 6746 7758 6746 Size: 1012 7674 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8686 BstBI TT'CG_AA Cuts at: 7490 7490 Size: 8686 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8686 BstXI CCAn_nnnn'nTGG Cuts at: 2080 4745 2080 Size: 2665 6021 BstYI r'GATC_y Cuts at: 1972 2782 5580 5726 6016 6383 6768 6785 8237 Size: 810 2798 146 290 367 385 17 1452 Cuts at: 8237 8323 8335 8681 1972 Size: 86 12 346 1977 Fragments arranged by size: 2798 1977 1452 810 385 367 346 290 146 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2740 2740 Size: 8686 BtgI C'CryG_G Cuts at: 513 1065 5885 513 Size: 552 4820 3314 Fragments arranged by size: 4820 3314 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7947 Fragments arranged by size: 7947 683 56 BtsI GCAGTG_nn' Cuts at: 2413 3612 3660 6202 6996 7016 2413 Size: 1199 48 2542 794 20 4083 Fragments arranged by size: 4083 2542 1199 794 48 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8686 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8651 DraI TTT'AAA Cuts at: 3807 3975 5990 3807 Size: 168 2015 6503 Fragments arranged by size: 6503 2015 168 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4876 7693 809 Size: 507 3560 2817 1802 Fragments arranged by size: 3560 2817 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4043 5970 6133 Size: 54 1425 50 275 18 2213 1927 163 Cuts at: 6133 7022 8651 8 Size: 889 1629 43 Fragments arranged by size: 2213 1927 1629 1425 889 275 163 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6133 1537 Size: 275 4321 4090 Fragments arranged by size: 4321 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2988 3114 3174 3213 3582 6616 1353 Size: 1635 126 60 39 369 3034 3423 Fragments arranged by size: 3423 3034 1635 369 126 60 39 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 7273 7647 7793 387 Size: 1064 138 5684 374 146 1280 Fragments arranged by size: 5684 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2250 2471 2583 3449 3774 4731 5427 Size: 393 618 221 112 866 325 957 696 Cuts at: 5427 5913 5967 6749 7346 8133 8401 1239 Size: 486 54 782 597 787 268 1524 Fragments arranged by size: 1524 957 866 787 782 696 618 597 486 393 325 268 221 112 54 EcoO109I rG'GnC_Cy Cuts at: 5819 5819 Size: 8686 EcoRI G'AATT_C Cuts at: 2620 5557 5995 6373 2620 Size: 2937 438 378 4933 Fragments arranged by size: 4933 2937 438 378 FspI TGC'GCA Cuts at: 7192 7192 Size: 8686 HaeII r_GCGC'y Cuts at: 1678 7833 1678 Size: 6155 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3053 3085 3179 3211 4792 Size: 32 135 32 1481 32 94 32 1581 Cuts at: 4792 4824 4877 4909 5342 5374 5395 5427 5572 Size: 32 53 32 433 32 21 32 145 Cuts at: 5572 5604 7327 7359 7401 7433 1373 Size: 32 1723 32 42 32 2626 Fragments arranged by size: 2626 1723 1581 1481 433 145 135 94 53 42 32 32 32 32 32 32 32 32 32 32 32 21 HincII GTy'rAC Cuts at: 669 1657 1938 2220 2516 3948 4150 6030 6276 Size: 988 281 282 296 1432 202 1880 246 Cuts at: 6276 669 Size: 3079 Fragments arranged by size: 3079 1880 1432 988 296 282 281 246 202 HindIII A'AGCT_T Cuts at: 748 4399 748 Size: 3651 5035 HpaI GTT'AAC Cuts at: 6276 6276 Size: 8686 KpnI G_GTAC'C Cuts at: 3519 6011 3519 Size: 2492 6194 MfeI C'AATT_G Cuts at: 3520 6285 3520 Size: 2765 5921 MluI A'CGCG_T Cuts at: 8562 8562 Size: 8686 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3109 4447 7800 7984 1305 Size: 1804 1338 3353 184 2007 Fragments arranged by size: 3353 2007 1804 1338 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 6864 Fragments arranged by size: 6864 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2298 3303 3330 3605 3838 4125 4233 Size: 648 1132 1005 27 275 233 287 108 Cuts at: 4233 4416 6644 7003 7162 518 Size: 183 2228 359 159 2042 Fragments arranged by size: 2228 2042 1132 1005 648 359 287 275 233 183 159 108 27 MspA1I CmG'CkG Cuts at: 1606 2480 2491 6102 6779 7927 8172 1606 Size: 874 11 3611 677 1148 245 2120 Fragments arranged by size: 3611 2120 1148 874 677 245 11 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 8134 NdeI CA'TA_TG Cuts at: 387 3288 3346 3402 3764 387 Size: 2901 58 56 362 5309 Fragments arranged by size: 5309 2901 362 58 56 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8686 NotI GC'GGCC_GC Cuts at: 6133 6133 Size: 8686 NsiI A_TGCA'T Cuts at: 3028 3028 Size: 8686 NspI r_CATG'y Cuts at: 1132 1229 2297 2555 3548 4099 4664 6044 7589 Size: 97 1068 258 993 551 565 1380 1545 Cuts at: 7589 1132 Size: 2229 Fragments arranged by size: 2229 1545 1380 1068 993 565 551 258 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8686 PciI A'CATG_T Cuts at: 4095 7585 4095 Size: 3490 5196 PflMI CCAn_nnn'nTGG Cuts at: 1108 5891 1108 Size: 4783 3903 PmeI GTTT'AAAC Cuts at: 5990 5990 Size: 8686 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6755 6787 8268 8300 6755 Size: 32 1481 32 7141 Fragments arranged by size: 7141 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 5819 5819 Size: 8686 PshAI GACnn'nnGTC Cuts at: 1613 4517 5824 1613 Size: 2904 1307 4475 Fragments arranged by size: 4475 2904 1307 PsiI TTA'TAA Cuts at: 3099 6256 3099 Size: 3157 5529 PstI C_TGCA'G Cuts at: 830 4831 6038 830 Size: 4001 1207 3478 Fragments arranged by size: 4001 3478 1207 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8686 PvuII CAG'CTG Cuts at: 2480 2491 2480 Size: 11 8675 SalI G'TCGA_C Cuts at: 1936 6028 1936 Size: 4092 4594 SapI GCTCTTCn'nnn_ Cuts at: 2988 3213 2988 Size: 225 8461 SbfI CC_TGCA'GG Cuts at: 6038 6038 Size: 8686 ScaI AGT'ACT Cuts at: 3824 6934 8646 3824 Size: 3110 1712 3864 Fragments arranged by size: 3864 3110 1712 SfcI C'TryA_G Cuts at: 826 1045 3729 3906 4243 4639 4827 5088 6034 Size: 219 2684 177 337 396 188 261 946 Cuts at: 6034 7169 7850 8041 826 Size: 1135 681 191 1471 Fragments arranged by size: 2684 1471 1135 946 681 396 337 261 219 191 188 177 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8686 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8686 SmaI CCC'GGG Cuts at: 1872 6013 1872 Size: 4141 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2263 4883 6790 7691 7953 820 Size: 197 926 320 2620 1907 901 262 1553 Fragments arranged by size: 2620 1907 1553 926 901 320 262 197 SnaBI TAC'GTA Cuts at: 493 6477 493 Size: 5984 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8686 SphI G_CATG'C Cuts at: 1132 6044 1132 Size: 4912 3774 SspI AAT'ATT Cuts at: 5 52 6610 5 Size: 47 6558 2081 Fragments arranged by size: 6558 2081 47 StuI AGG'CCT Cuts at: 1517 4393 1517 Size: 2876 5810 StyI C'CwwG_G Cuts at: 513 1065 3338 4200 6125 513 Size: 552 2273 862 1925 3074 Fragments arranged by size: 3074 2273 1925 862 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2582 2666 6713 6730 6883 7068 7513 Size: 1255 1002 84 4047 17 153 185 445 Cuts at: 7513 325 Size: 1498 Fragments arranged by size: 4047 1498 1255 1002 445 185 153 84 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3239 3822 6932 Size: 274 80 33 51 1443 1262 583 3110 Cuts at: 6932 8644 96 Size: 1712 138 Fragments arranged by size: 3110 1712 1443 1262 583 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2418 2450 8646 8678 2418 Size: 32 6196 32 2426 Fragments arranged by size: 6196 2426 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8686 XbaI T'CTAG_A Cuts at: 2045 2863 2980 6022 2045 Size: 818 117 3042 4709 Fragments arranged by size: 4709 3042 818 117 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8686 XmaI C'CCGG_G Cuts at: 1870 6011 1870 Size: 4141 4545 XmnI GAAnn'nnTTC Cuts at: 6815 6815 Size: 8686 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6494 276 Size: 53 83 186 936 4960 2468 Fragments arranged by size: 4960 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI