Restriction map for
pFN21AA1221
(Linear) (Six-base) MAPSORT of: KIAA1221.seq Check: 8717 from: 1 to: 3981
KIAA1221.orf
With 182 enzymes: SgfI *
February 28, 2007 11:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1203 3981
Size: 1203 2778
AccI GT'mk_AC
Cuts at: 0 490 968 1004 3981
Size: 490 478 36 2977
Fragments arranged by size:
2977 490 478 36
Acc65I G'GTAC_C
Cuts at: 0 1510 3981
Size: 1510 2471
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 245 466 578 1444 1769 3422 3981
Size: 245 221 112 866 325 1653 559
Fragments arranged by size:
1653 866 559 325 245 221 112
AflII C'TTAA_G
Cuts at: 0 258 3981
Size: 258 3723
AflIII A'CryG_T
Cuts at: 0 2090 2412 3981
Size: 2090 322 1569
Fragments arranged by size:
2090 1569 322
AleI CACnn'nnGTG
Cuts at: 0 2411 3981
Size: 2411 1570
AlwNI CAG_nnn'CTG
Cuts at: 0 3418 3981
Size: 3418 563
ApaLI G'TGCA_C
Cuts at: 0 1478 3981
Size: 1478 2503
ApoI r'AATT_y
Cuts at: 0 615 728 1251 1439 2027 2307 2661 3441
Size: 615 113 523 188 588 280 354 780
Cuts at: 3441 3552 3981
Size: 111 429
Fragments arranged by size:
780 615 588 523 429 354 280 188
113 111
AseI AT'TA_AT
Cuts at: 0 2645 3981
Size: 2645 1336
BamHI G'GATC_C
Cuts at: 0 777 3981
Size: 777 3204
BanI G'GyrC_C
Cuts at: 0 1510 3981
Size: 1510 2471
BbsI GAAGACnn'nnnn_
Cuts at: 0 1061 1425 2477 3839 3981
Size: 1061 364 1052 1362 142
Fragments arranged by size:
1362 1061 1052 364 142
BbvCI CC'TCA_GC
Cuts at: 0 2807 3981
Size: 2807 1174
BclI T'GATC_A
Cuts at: 0 2246 3981
Size: 2246 1735
BfrBI ATG'CAT
Cuts at: 0 1021 3981
Size: 1021 2960
BglI GCCn_nnn'nGGC
Cuts at: 0 1631 3981
Size: 1631 2350
BglII A'GATC_T
Cuts at: 0 3721 3981
Size: 3721 260
Bme1580I G_kGCm'C
Cuts at: 0 1482 3981
Size: 1482 2499
BmgBI CAC'GTC
Cuts at: 0 2413 3911 3981
Size: 2413 1498 70
Fragments arranged by size:
2413 1498 70
BmrI ACTGGGnnnn_n'
Cuts at: 0 783 3476 3981
Size: 783 2693 505
Fragments arranged by size:
2693 783 505
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2726 3908 3981
Size: 2726 1182 73
Fragments arranged by size:
2726 1182 73
Bpu10I CC'TnA_GC
Cuts at: 0 2807 3981
Size: 2807 1174
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2899 3981
Size: 2899 1082
BsaBI GATnn'nnATC
Cuts at: 0 2483 2645 3981
Size: 2483 162 1336
Fragments arranged by size:
2483 1336 162
BsaHI Gr'CG_yC
Cuts at: 0 2378 3981
Size: 2378 1603
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 329 359 3981
Size: 329 30 3622
Fragments arranged by size:
3622 329 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 357 2102 3557 3981
Size: 357 1745 1455 424
Fragments arranged by size:
1745 1455 424 357
BseYI C'CCAG_C
Cuts at: 0 2904 3981
Size: 2904 1077
BsiHKAI G_wGCw'C
Cuts at: 0 300 1482 3981
Size: 300 1182 2499
Fragments arranged by size:
2499 1182 300
BsmI GAATG_Cn'
Cuts at: 0 1627 1824 3981
Size: 1627 197 2157
Fragments arranged by size:
2157 1627 197
Bsp1286I G_dGCh'C
Cuts at: 0 300 1482 3981
Size: 300 1182 2499
Fragments arranged by size:
2499 1182 300
BspHI T'CATG_A
Cuts at: 0 1712 3981
Size: 1712 2269
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1203 3779 3981
Size: 1203 2576 202
Fragments arranged by size:
2576 1203 202
BsrBI CCG'CTC
Cuts at: 0 161 3981
Size: 161 3820
BsrDI GCAATG_nn'
Cuts at: 0 1251 1634 2069 3981
Size: 1251 383 435 1912
Fragments arranged by size:
1912 1251 435 383
BsrGI T'GTAC_A
Cuts at: 0 1234 3981
Size: 1234 2747
BstXI CCAn_nnnn'nTGG
Cuts at: 0 75 2740 3981
Size: 75 2665 1241
Fragments arranged by size:
2665 1241 75
BstYI r'GATC_y
Cuts at: 0 777 3575 3721 3981
Size: 777 2798 146 260
Fragments arranged by size:
2798 777 260 146
Bsu36I CC'TnA_GG
Cuts at: 0 735 3981
Size: 735 3246
BtgI C'CryG_G
Cuts at: 0 3880 3981
Size: 3880 101
BtsI GCAGTG_nn'
Cuts at: 0 408 1607 1655 3981
Size: 408 1199 48 2326
Fragments arranged by size:
2326 1199 408 48
DraI TTT'AAA
Cuts at: 0 1802 1970 3981
Size: 1802 168 2011
Fragments arranged by size:
2011 1802 168
DrdI GACnn_nn'nnGTC
Cuts at: 0 2871 3981
Size: 2871 1110
EaeI y'GGCC_r
Cuts at: 0 2038 3965 3981
Size: 2038 1927 16
Fragments arranged by size:
2038 1927 16
EarI CTCTTCn'nnn_
Cuts at: 0 983 1109 1169 1208 1577 3981
Size: 983 126 60 39 369 2404
Fragments arranged by size:
2404 983 369 126 60 39
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 245 466 578 1444 1769 2726 3422 3908
Size: 245 221 112 866 325 957 696 486
Cuts at: 3908 3981
Size: 73
Fragments arranged by size:
957 866 696 486 325 245 221 112
73
EcoO109I rG'GnC_Cy
Cuts at: 0 3814 3981
Size: 3814 167
EcoRI G'AATT_C
Cuts at: 0 615 3552 3981
Size: 615 2937 429
Fragments arranged by size:
2937 615 429
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1048 1080 1174 1206 2787 2819 2872 2904
Size: 1048 32 94 32 1581 32 53 32
Cuts at: 2904 3337 3369 3390 3422 3567 3599 3981
Size: 433 32 21 32 145 32 382
Fragments arranged by size:
1581 1048 433 382 145 94 53 32
32 32 32 32 32 32 21
HincII GTy'rAC
Cuts at: 0 215 511 1943 2145 3981
Size: 215 296 1432 202 1836
Fragments arranged by size:
1836 1432 296 215 202
HindIII A'AGCT_T
Cuts at: 0 2394 3981
Size: 2394 1587
KpnI G_GTAC'C
Cuts at: 0 1514 3981
Size: 1514 2467
MfeI C'AATT_G
Cuts at: 0 1515 3981
Size: 1515 2466
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1104 2442 3981
Size: 1104 1338 1539
Fragments arranged by size:
1539 1338 1104
MslI CAynn'nnrTG
Cuts at: 0 293 1298 1325 1600 1833 2120 2228 2411
Size: 293 1005 27 275 233 287 108 183
Cuts at: 2411 3981
Size: 1570
Fragments arranged by size:
1570 1005 293 287 275 233 183 108
27
MspA1I CmG'CkG
Cuts at: 0 475 486 3981
Size: 475 11 3495
Fragments arranged by size:
3495 475 11
NdeI CA'TA_TG
Cuts at: 0 1283 1341 1397 1759 3981
Size: 1283 58 56 362 2222
Fragments arranged by size:
2222 1283 362 58 56
NsiI A_TGCA'T
Cuts at: 0 1023 3981
Size: 1023 2958
NspI r_CATG'y
Cuts at: 0 292 550 1543 2094 2659 3981
Size: 292 258 993 551 565 1322
Fragments arranged by size:
1322 993 565 551 292 258
PciI A'CATG_T
Cuts at: 0 2090 3981
Size: 2090 1891
PflMI CCAn_nnn'nTGG
Cuts at: 0 3886 3981
Size: 3886 95
PpuMI rG'GwC_Cy
Cuts at: 0 3814 3981
Size: 3814 167
PshAI GACnn'nnGTC
Cuts at: 0 2512 3819 3981
Size: 2512 1307 162
Fragments arranged by size:
2512 1307 162
PsiI TTA'TAA
Cuts at: 0 1094 3981
Size: 1094 2887
PstI C_TGCA'G
Cuts at: 0 2826 3981
Size: 2826 1155
PvuII CAG'CTG
Cuts at: 0 475 486 3981
Size: 475 11 3495
Fragments arranged by size:
3495 475 11
SapI GCTCTTCn'nnn_
Cuts at: 0 983 1208 3981
Size: 983 225 2773
Fragments arranged by size:
2773 983 225
ScaI AGT'ACT
Cuts at: 0 1819 3981
Size: 1819 2162
SfcI C'TryA_G
Cuts at: 0 1724 1901 2238 2634 2822 3083 3981
Size: 1724 177 337 396 188 261 898
Fragments arranged by size:
1724 898 396 337 261 188 177
SmlI C'TyrA_G
Cuts at: 0 258 2878 3981
Size: 258 2620 1103
Fragments arranged by size:
2620 1103 258
StuI AGG'CCT
Cuts at: 0 2388 3981
Size: 2388 1593
StyI C'CwwG_G
Cuts at: 0 1333 2195 3981
Size: 1333 862 1786
Fragments arranged by size:
1786 1333 862
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 577 661 3981
Size: 577 84 3320
Fragments arranged by size:
3320 577 84
TatI w'GTAC_w
Cuts at: 0 1234 1817 3981
Size: 1234 583 2164
Fragments arranged by size:
2164 1234 583
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 413 445 3981
Size: 413 32 3536
Fragments arranged by size:
3536 413 32
XbaI T'CTAG_A
Cuts at: 0 40 858 975 3981
Size: 40 818 117 3006
Fragments arranged by size:
3006 818 117 40
Enzymes that do cut:
AarI AccI Acc65I AcuI AflII AflIII AleI AlwNI
ApaLI ApoI AseI BamHI BanI BbsI BbvCI BclI
BfrBI BglI BglII Bme1580I BmgBI BmrI BpmI Bpu10I
BpuEI BsaBI BsaHI BsaXI BseRI BseYI BsiHKAI BsmI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrGI BstXI BstYI
Bsu36I BtgI BtsI DraI DrdI EaeI EarI Eco57MI
EcoO109I EcoRI Hin4I HincII HindIII KpnI MfeI MmeI
MslI MspA1I NdeI NsiI NspI PciI PflMI PpuMI
PshAI PsiI PstI PvuII SapI ScaI SfcI SmlI
StuI StyI TaqII TatI TstI XbaI
Enzymes that do not cut:
AatII AclI AfeI AgeI AhdI AlfI AloI ApaI
AscI AsiSI AvaI AvrII BaeI BanII BbeI BcgI
BciVI BlpI BmtI BplI BsaI BsaAI BsaWI BsgI
BsiEI BsiWI BsmBI BspEI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstZ17I BtgZI ClaI CspCI DraIII EagI
EciI EcoICRI EcoNI EcoRV FalI FseI FspI FspAI
HaeII HpaI KasI MluI MscI NaeI NarI NcoI
NgoMIV NheI NotI NruI PacI PasI PfoI PmeI
PmlI PpiI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SbfI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI
Tth111I XcmI XhoI XmaI XmnI ZraI