(Circular) (Six-base) MAPSORT of: pFN21AA1180.seq Check: 8847 from: 1 to: 5761 pFN21AA1180 5761 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3571 278 Size: 53 83 186 936 2035 2468 Fragments arranged by size: 2468 2035 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3104 1937 Size: 1167 4594 Acc65I G'GTAC_C Cuts at: 2253 3082 2253 Size: 829 4932 AclI AA'CG_TT Cuts at: 1563 3888 4261 1563 Size: 2325 373 3063 Fragments arranged by size: 3063 2325 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2497 2798 2853 3824 5208 5476 1632 Size: 865 301 55 971 1384 268 1917 Fragments arranged by size: 1917 1384 971 865 301 268 55 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5564 AflIII A'CryG_T Cuts at: 1203 2613 4660 5637 1203 Size: 1410 2047 977 1327 Fragments arranged by size: 2047 1410 1327 977 AgeI A'CCGG_T Cuts at: 4592 4592 Size: 5761 AhdI GACnn_n'nnGTC Cuts at: 4490 4490 Size: 5761 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5761 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2298 1184 Size: 1114 4647 AlwNI CAG_nnn'CTG Cuts at: 2792 3504 5076 2792 Size: 712 1572 3477 Fragments arranged by size: 3477 1572 712 ApaLI G'TGCA_C Cuts at: 3818 4974 3818 Size: 1156 4605 ApoI r'AATT_y Cuts at: 1493 3070 3296 3448 5557 1493 Size: 1577 226 152 2109 1697 Fragments arranged by size: 2109 1697 1577 226 152 AseI AT'TA_AT Cuts at: 160 1032 4315 160 Size: 872 3283 1606 Fragments arranged by size: 3283 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5761 AvaI C'yCGr_G Cuts at: 1870 1943 3086 1870 Size: 73 1143 4545 Fragments arranged by size: 4545 1143 73 BamHI G'GATC_C Cuts at: 3091 3458 3091 Size: 367 5394 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2253 2345 2659 3082 4537 Size: 325 217 627 466 92 314 423 1455 Cuts at: 4537 618 Size: 1842 Fragments arranged by size: 1842 1455 627 466 423 325 314 217 92 BanII G_rGCy'C Cuts at: 1391 2078 3080 1391 Size: 687 1002 4072 Fragments arranged by size: 4072 1002 687 BbeI G_GCGC'C Cuts at: 2349 2349 Size: 5761 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4790 BbvCI CC'TCA_GC Cuts at: 2721 2914 2721 Size: 193 5568 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3952 3986 1903 Size: 34 50 34 1931 34 3678 Fragments arranged by size: 3678 1931 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3653 4863 3653 Size: 1210 4551 BclI T'GATC_A Cuts at: 1548 1548 Size: 5761 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4372 136 Size: 107 122 71 631 605 2700 1525 Fragments arranged by size: 2700 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5756 5756 Size: 5761 BlpI GC'TnA_GC Cuts at: 3178 3178 Size: 5761 Bme1580I G_kGCm'C Cuts at: 1790 2664 2913 3822 4978 1790 Size: 874 249 909 1156 2573 Fragments arranged by size: 2573 1156 909 874 249 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5761 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2458 3492 4450 Size: 416 53 83 399 24 1041 1034 958 Cuts at: 4450 442 Size: 1753 Fragments arranged by size: 1753 1041 1034 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5761 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2761 2761 Size: 5761 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2814 4421 1239 Size: 1575 1607 2579 Fragments arranged by size: 2579 1607 1575 Bpu10I CC'TnA_GC Cuts at: 2721 2914 2721 Size: 193 5568 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3886 4751 5049 3886 Size: 865 298 4598 Fragments arranged by size: 4598 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2625 4424 882 Size: 617 1126 1799 2219 Fragments arranged by size: 2219 1799 1126 617 BsaAI yAC'GTr Cuts at: 493 1204 2206 3552 493 Size: 711 1002 1346 2702 Fragments arranged by size: 2702 1346 1002 711 BsaBI GATnn'nnATC Cuts at: 2333 5755 2333 Size: 3422 2339 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2234 2346 3568 3950 Size: 53 83 186 936 701 112 1222 382 Cuts at: 3950 4585 275 Size: 635 1451 Fragments arranged by size: 1451 1222 936 701 635 382 186 112 83 53 BsaWI w'CCGG_w Cuts at: 4194 4592 4866 5013 5424 4194 Size: 398 274 147 411 4531 Fragments arranged by size: 4531 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2343 2373 2892 2922 1178 Size: 30 165 30 940 30 519 30 4017 Fragments arranged by size: 4017 940 519 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2537 2916 1186 Size: 1351 379 4031 Fragments arranged by size: 4031 1351 379 BseYI C'CCAG_C Cuts at: 2016 2095 2294 3025 4964 5460 2016 Size: 79 199 731 1939 496 2317 Fragments arranged by size: 2317 1939 731 496 199 79 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2324 2570 2983 1163 Size: 573 588 246 413 3941 Fragments arranged by size: 3941 588 573 413 246 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3211 3972 5000 1540 Size: 275 186 1210 761 1028 2301 Fragments arranged by size: 2301 1210 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2502 3822 3907 4978 2502 Size: 1320 85 1071 3285 Fragments arranged by size: 3285 1320 1071 85 BsmI GAATG_Cn' Cuts at: 3272 3365 3272 Size: 93 5668 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2078 2502 2664 2913 3080 3822 3907 Size: 399 288 424 162 249 167 742 85 Cuts at: 3907 4978 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 424 399 288 249 167 162 85 BspEI T'CCGG_A Cuts at: 5424 5424 Size: 5761 BspHI T'CATG_A Cuts at: 3650 5380 3650 Size: 1730 4031 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2604 3116 844 Size: 1760 512 3489 Fragments arranged by size: 3489 1760 512 BsrBI CCG'CTC Cuts at: 1394 1823 3648 1394 Size: 429 1825 3507 Fragments arranged by size: 3507 1825 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2868 4256 4430 65 Size: 1183 1620 1388 174 1396 Fragments arranged by size: 1620 1396 1388 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2559 2646 4405 4592 1700 Size: 110 749 87 1759 187 2869 Fragments arranged by size: 2869 1759 749 187 110 87 BsrGI T'GTAC_A Cuts at: 96 2616 96 Size: 2520 3241 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5761 BssSI C'ACGA_G Cuts at: 3821 4833 3821 Size: 1012 4749 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5761 BstBI TT'CG_AA Cuts at: 4565 4565 Size: 5761 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5761 BstXI CCAn_nnnn'nTGG Cuts at: 2974 2974 Size: 5761 BstYI r'GATC_y Cuts at: 1972 3091 3458 3843 3860 5312 5398 5410 5756 Size: 1119 367 385 17 1452 86 12 346 Cuts at: 5756 1972 Size: 1977 Fragments arranged by size: 1977 1452 1119 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2247 2247 Size: 5761 BtgI C'CryG_G Cuts at: 513 1065 2268 3045 513 Size: 552 1203 777 3229 Fragments arranged by size: 3229 1203 777 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5022 Fragments arranged by size: 5022 683 56 BtsI GCAGTG_nn' Cuts at: 2913 2946 3277 4071 4091 2913 Size: 33 331 794 20 4583 Fragments arranged by size: 4583 794 331 33 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5761 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5726 DraI TTT'AAA Cuts at: 3065 3065 Size: 5761 DraIII CAC_nnn'GTG Cuts at: 2194 2194 Size: 5761 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4768 809 Size: 507 3452 1802 Fragments arranged by size: 3452 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2364 3208 4097 Size: 54 1425 50 275 18 534 844 889 Cuts at: 4097 5726 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 844 534 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3208 1537 Size: 275 1396 4090 Fragments arranged by size: 4090 1396 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2516 2609 3691 1353 Size: 1163 93 1082 3423 Fragments arranged by size: 3423 1163 1082 93 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4348 4722 4868 387 Size: 1064 138 2759 374 146 1280 Fragments arranged by size: 2759 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2497 2798 2814 2853 3824 4421 5208 Size: 393 865 301 16 39 971 597 787 Cuts at: 5208 5476 1239 Size: 268 1524 Fragments arranged by size: 1524 971 865 787 597 393 301 268 39 16 EcoNI CCTnn'n_nnAGG Cuts at: 2396 2396 Size: 5761 EcoRI G'AATT_C Cuts at: 3070 3448 3070 Size: 378 5383 FspI TGC'GCA Cuts at: 4267 4267 Size: 5761 HaeII r_GCGC'y Cuts at: 1678 2349 4908 1678 Size: 671 2559 2531 Fragments arranged by size: 2559 2531 671 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4402 4434 4476 4508 1373 Size: 32 135 32 2830 32 42 32 2626 Fragments arranged by size: 2830 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3037 3105 3351 669 Size: 988 281 1099 68 246 3079 Fragments arranged by size: 3079 1099 988 281 246 68 HindIII A'AGCT_T Cuts at: 748 748 Size: 5761 HpaI GTT'AAC Cuts at: 3351 3351 Size: 5761 KasI G'GCGC_C Cuts at: 2345 2345 Size: 5761 KpnI G_GTAC'C Cuts at: 2257 3086 2257 Size: 829 4932 MfeI C'AATT_G Cuts at: 3360 3360 Size: 5761 MluI A'CGCG_T Cuts at: 5637 5637 Size: 5761 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4875 5059 1305 Size: 3570 184 2007 Fragments arranged by size: 3570 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2366 10 Size: 54 1425 343 534 3405 Fragments arranged by size: 3405 1425 534 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2208 2339 3050 3719 4078 4237 518 Size: 648 1042 131 711 669 359 159 2042 Fragments arranged by size: 2042 1042 711 669 648 359 159 131 MspA1I CmG'CkG Cuts at: 1606 2278 2682 2720 3177 3854 5002 5247 1606 Size: 672 404 38 457 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 672 457 404 245 38 NarI GG'CG_CC Cuts at: 2346 2346 Size: 5761 NcoI C'CATG_G Cuts at: 513 1065 2268 3045 513 Size: 552 1203 777 3229 Fragments arranged by size: 3229 1203 777 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5761 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5761 NotI GC'GGCC_GC Cuts at: 3208 3208 Size: 5761 NspI r_CATG'y Cuts at: 1132 1229 2063 2173 2617 3119 4664 1132 Size: 97 834 110 444 502 1545 2229 Fragments arranged by size: 2229 1545 834 502 444 110 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5761 PciI A'CATG_T Cuts at: 2613 4660 2613 Size: 2047 3714 PflMI CCAn_nnn'nTGG Cuts at: 1108 2119 3046 1108 Size: 1011 927 3823 Fragments arranged by size: 3823 1011 927 PmeI GTTT'AAAC Cuts at: 3065 3065 Size: 5761 PmlI CAC'GTG Cuts at: 2206 2206 Size: 5761 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3830 3862 5343 5375 3830 Size: 32 1481 32 4216 Fragments arranged by size: 4216 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5761 PsiI TTA'TAA Cuts at: 3331 3331 Size: 5761 PstI C_TGCA'G Cuts at: 830 2601 3113 830 Size: 1771 512 3478 Fragments arranged by size: 3478 1771 512 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5761 PvuII CAG'CTG Cuts at: 2278 2278 Size: 5761 SalI G'TCGA_C Cuts at: 1936 3103 1936 Size: 1167 4594 SapI GCTCTTCn'nnn_ Cuts at: 2609 2609 Size: 5761 SbfI CC_TGCA'GG Cuts at: 3113 3113 Size: 5761 ScaI AGT'ACT Cuts at: 4009 5721 4009 Size: 1712 4049 SexAI A'CCwGG_T Cuts at: 2391 2391 Size: 5761 SfcI C'TryA_G Cuts at: 826 1045 2597 3109 4244 4925 5116 826 Size: 219 1552 512 1135 681 191 1471 Fragments arranged by size: 1552 1471 1135 681 512 219 191 SfoI GGC'GCC Cuts at: 2347 2347 Size: 5761 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5761 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5761 SmaI CCC'GGG Cuts at: 1872 3088 1872 Size: 1216 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3865 4766 5028 820 Size: 197 926 1922 901 262 1553 Fragments arranged by size: 1922 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3552 493 Size: 3059 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5761 SphI G_CATG'C Cuts at: 1132 3119 1132 Size: 1987 3774 SspI AAT'ATT Cuts at: 5 52 3685 5 Size: 47 3633 2081 Fragments arranged by size: 3633 2081 47 StuI AGG'CCT Cuts at: 1517 2996 1517 Size: 1479 4282 StyI C'CwwG_G Cuts at: 513 1065 2082 2268 3045 3200 513 Size: 552 1017 186 777 155 3074 Fragments arranged by size: 3074 1017 777 552 186 155 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2901 3017 3788 3805 3958 4143 4588 Size: 1255 1321 116 771 17 153 185 445 Cuts at: 4588 325 Size: 1498 Fragments arranged by size: 1498 1321 1255 771 445 185 153 116 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2616 4007 5719 Size: 274 80 33 51 1443 639 1391 1712 Cuts at: 5719 96 Size: 138 Fragments arranged by size: 1712 1443 1391 639 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2211 2243 2343 2367 2375 2399 2892 2924 5721 Size: 32 100 24 8 24 493 32 2797 Cuts at: 5721 5753 2211 Size: 32 2219 Fragments arranged by size: 2797 2219 493 100 32 32 32 24 24 8 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5761 XbaI T'CTAG_A Cuts at: 3097 3097 Size: 5761 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2191 2275 2191 Size: 84 5677 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5761 XmaI C'CCGG_G Cuts at: 1870 3086 1870 Size: 1216 4545 XmnI GAAnn'nnTTC Cuts at: 3890 3890 Size: 5761 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3569 276 Size: 53 83 186 936 2035 2468 Fragments arranged by size: 2468 2035 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I EcoICRI EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SfiI SrfI SwaI