Restriction map for
pFN21AA1180
(Linear) (Six-base) MAPSORT of: KIAA1180.seq Check: 2511 from: 1 to: 1056
KIAA1180.orf
With 182 enzymes: SgfI *
February 28, 2007 11:22 ..
Acc65I G'GTAC_C
Cuts at: 0 248 1056
Size: 248 808
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 492 793 848 1056
Size: 492 301 55 208
Fragments arranged by size:
492 301 208 55
AflIII A'CryG_T
Cuts at: 0 608 1056
Size: 608 448
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 293 1056
Size: 293 763
AlwNI CAG_nnn'CTG
Cuts at: 0 787 1056
Size: 787 269
BanI G'GyrC_C
Cuts at: 0 248 340 654 1056
Size: 248 92 314 402
Fragments arranged by size:
402 314 248 92
BanII G_rGCy'C
Cuts at: 0 73 1056
Size: 73 983
BbeI G_GCGC'C
Cuts at: 0 344 1056
Size: 344 712
BbvCI CC'TCA_GC
Cuts at: 0 716 909 1056
Size: 716 193 147
Fragments arranged by size:
716 193 147
Bme1580I G_kGCm'C
Cuts at: 0 659 908 1056
Size: 659 249 148
Fragments arranged by size:
659 249 148
BmrI ACTGGGnnnn_n'
Cuts at: 0 453 1056
Size: 453 603
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 756 1056
Size: 756 300
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 809 1056
Size: 809 247
Bpu10I CC'TnA_GC
Cuts at: 0 716 909 1056
Size: 716 193 147
Fragments arranged by size:
716 193 147
BsaI GGTCTCn'nnnn_
Cuts at: 0 620 1056
Size: 620 436
BsaAI yAC'GTr
Cuts at: 0 201 1056
Size: 201 855
BsaBI GATnn'nnATC
Cuts at: 0 328 1056
Size: 328 728
BsaHI Gr'CG_yC
Cuts at: 0 229 341 1056
Size: 229 112 715
Fragments arranged by size:
715 229 112
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 338 368 887 917 1056
Size: 338 30 519 30 139
Fragments arranged by size:
519 338 139 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 532 911 1056
Size: 532 379 145
Fragments arranged by size:
532 379 145
BseYI C'CCAG_C
Cuts at: 0 11 90 289 1020 1056
Size: 11 79 199 731 36
Fragments arranged by size:
731 199 79 36 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 319 565 978 1056
Size: 319 246 413 78
Fragments arranged by size:
413 319 246 78
BsiHKAI G_wGCw'C
Cuts at: 0 497 1056
Size: 497 559
Bsp1286I G_dGCh'C
Cuts at: 0 73 497 659 908 1056
Size: 73 424 162 249 148
Fragments arranged by size:
424 249 162 148 73
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 599 1056
Size: 599 457
BsrDI GCAATG_nn'
Cuts at: 0 863 1056
Size: 863 193
BsrFI r'CCGG_y
Cuts at: 0 554 641 1056
Size: 554 87 415
Fragments arranged by size:
554 415 87
BsrGI T'GTAC_A
Cuts at: 0 611 1056
Size: 611 445
BstXI CCAn_nnnn'nTGG
Cuts at: 0 969 1056
Size: 969 87
Bsu36I CC'TnA_GG
Cuts at: 0 242 1056
Size: 242 814
BtgI C'CryG_G
Cuts at: 0 263 1040 1056
Size: 263 777 16
Fragments arranged by size:
777 263 16
BtsI GCAGTG_nn'
Cuts at: 0 908 941 1056
Size: 908 33 115
Fragments arranged by size:
908 115 33
DraIII CAC_nnn'GTG
Cuts at: 0 189 1056
Size: 189 867
EaeI y'GGCC_r
Cuts at: 0 359 1056
Size: 359 697
EarI CTCTTCn'nnn_
Cuts at: 0 511 604 1056
Size: 511 93 452
Fragments arranged by size:
511 452 93
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 492 793 809 848 1056
Size: 492 301 16 39 208
Fragments arranged by size:
492 301 208 39 16
EcoNI CCTnn'n_nnAGG
Cuts at: 0 391 1056
Size: 391 665
HaeII r_GCGC'y
Cuts at: 0 344 1056
Size: 344 712
HincII GTy'rAC
Cuts at: 0 1032 1056
Size: 1032 24
KasI G'GCGC_C
Cuts at: 0 340 1056
Size: 340 716
KpnI G_GTAC'C
Cuts at: 0 252 1056
Size: 252 804
MscI TGG'CCA
Cuts at: 0 361 1056
Size: 361 695
MslI CAynn'nnrTG
Cuts at: 0 203 334 1045 1056
Size: 203 131 711 11
Fragments arranged by size:
711 203 131 11
MspA1I CmG'CkG
Cuts at: 0 273 677 715 1056
Size: 273 404 38 341
Fragments arranged by size:
404 341 273 38
NarI GG'CG_CC
Cuts at: 0 341 1056
Size: 341 715
NcoI C'CATG_G
Cuts at: 0 263 1040 1056
Size: 263 777 16
Fragments arranged by size:
777 263 16
NspI r_CATG'y
Cuts at: 0 58 168 612 1056
Size: 58 110 444 444
Fragments arranged by size:
444 444 110 58
PciI A'CATG_T
Cuts at: 0 608 1056
Size: 608 448
PflMI CCAn_nnn'nTGG
Cuts at: 0 114 1041 1056
Size: 114 927 15
Fragments arranged by size:
927 114 15
PmlI CAC'GTG
Cuts at: 0 201 1056
Size: 201 855
PstI C_TGCA'G
Cuts at: 0 596 1056
Size: 596 460
PvuII CAG'CTG
Cuts at: 0 273 1056
Size: 273 783
SapI GCTCTTCn'nnn_
Cuts at: 0 604 1056
Size: 604 452
SexAI A'CCwGG_T
Cuts at: 0 386 1056
Size: 386 670
SfcI C'TryA_G
Cuts at: 0 592 1056
Size: 592 464
SfoI GGC'GCC
Cuts at: 0 342 1056
Size: 342 714
StuI AGG'CCT
Cuts at: 0 991 1056
Size: 991 65
StyI C'CwwG_G
Cuts at: 0 77 263 1040 1056
Size: 77 186 777 16
Fragments arranged by size:
777 186 77 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 896 1012 1056
Size: 896 116 44
Fragments arranged by size:
896 116 44
TatI w'GTAC_w
Cuts at: 0 611 1056
Size: 611 445
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 206 238 338 362 370 394 887 919
Size: 206 32 100 24 8 24 493 32
Cuts at: 919 1056
Size: 137
Fragments arranged by size:
493 206 137 100 32 32 24 24
8
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 186 270 1056
Size: 186 84 786
Fragments arranged by size:
786 186 84
Enzymes that do cut:
Acc65I AcuI AflIII AlfI AlwNI BanI BanII BbeI
BbvCI Bme1580I BmrI BplI BpmI Bpu10I BsaI BsaAI
BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspMI BsrDI BsrFI BsrGI BstXI Bsu36I BtgI BtsI
DraIII EaeI EarI Eco57MI EcoNI HaeII HincII KasI
KpnI MscI MslI MspA1I NarI NcoI NspI PciI
PflMI PmlI PstI PvuII SapI SexAI SfcI SfoI
StuI StyI TaqII TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AhdI
AleI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI BmgBI BmtI BpuEI BsaWI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BssHII
BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgZI ClaI
CspCI DraI DrdI EagI EciI EcoICRI EcoO109I EcoRI
EcoRV FalI FseI FspI FspAI Hin4I HindIII HpaI
MfeI MluI MmeI NaeI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SbfI ScaI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI
Tth111I XbaI XhoI XmaI XmnI ZraI