(Circular) (Six-base) MAPSORT of: pFN21AA1122.seq Check: 5152 from: 1 to: 7789 pFN21AA1122 7789 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2597 2597 Size: 7789 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5599 278 Size: 53 83 186 936 4063 2468 Fragments arranged by size: 4063 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5132 1937 Size: 3195 4594 Acc65I G'GTAC_C Cuts at: 4698 5110 4698 Size: 412 7377 AclI AA'CG_TT Cuts at: 1563 5916 6289 1563 Size: 4353 373 3063 Fragments arranged by size: 4353 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2145 2160 2406 2474 2628 3923 4812 5852 Size: 513 15 246 68 154 1295 889 1040 Cuts at: 5852 7236 7504 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 1295 1040 889 513 268 246 154 68 15 AflII C'TTAA_G Cuts at: 820 1017 2537 820 Size: 197 1520 6072 Fragments arranged by size: 6072 1520 197 AflIII A'CryG_T Cuts at: 1203 4035 4501 6688 7665 1203 Size: 2832 466 2187 977 1327 Fragments arranged by size: 2832 2187 1327 977 466 AgeI A'CCGG_T Cuts at: 6620 6620 Size: 7789 AhdI GACnn_n'nnGTC Cuts at: 6518 6518 Size: 7789 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7789 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7789 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3269 3301 3269 Size: 32 7757 AlwNI CAG_nnn'CTG Cuts at: 3095 5532 7104 3095 Size: 2437 1572 3780 Fragments arranged by size: 3780 2437 1572 ApaI G_GGCC'C Cuts at: 2619 2619 Size: 7789 ApaLI G'TGCA_C Cuts at: 5846 7002 5846 Size: 1156 6633 ApoI r'AATT_y Cuts at: 1493 2666 2789 2996 3168 4018 4574 5098 5324 Size: 1173 123 207 172 850 556 524 226 Cuts at: 5324 5476 7585 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1173 850 556 524 226 207 172 152 123 AseI AT'TA_AT Cuts at: 160 1032 3945 4530 6343 160 Size: 872 2913 585 1813 1606 Fragments arranged by size: 2913 1813 1606 872 585 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7789 AvaI C'yCGr_G Cuts at: 1870 1943 5114 1870 Size: 73 3171 4545 Fragments arranged by size: 4545 3171 73 AvrII C'CTAG_G Cuts at: 3580 3580 Size: 7789 BamHI G'GATC_C Cuts at: 5119 5486 5119 Size: 367 7422 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3705 4698 5110 6565 618 Size: 325 217 627 1918 993 412 1455 1842 Fragments arranged by size: 1918 1842 1455 993 627 412 325 217 BanII G_rGCy'C Cuts at: 1391 2619 3917 5108 1391 Size: 1228 1298 1191 4072 Fragments arranged by size: 4072 1298 1228 1191 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2280 2457 2475 2892 3078 3399 928 Size: 971 381 177 18 417 186 321 5318 Fragments arranged by size: 5318 971 417 381 321 186 177 18 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5980 6014 1903 Size: 34 4043 34 3678 Fragments arranged by size: 4043 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5681 6891 5681 Size: 1210 6579 BclI T'GATC_A Cuts at: 1548 2677 1548 Size: 1129 6660 BfrBI ATG'CAT Cuts at: 4593 4593 Size: 7789 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6400 136 Size: 107 122 71 631 605 4728 1525 Fragments arranged by size: 4728 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7784 7784 Size: 7789 BlpI GC'TnA_GC Cuts at: 5206 5206 Size: 7789 Bme1580I G_kGCm'C Cuts at: 1790 2619 3710 5850 7006 1790 Size: 829 1091 2140 1156 2573 Fragments arranged by size: 2573 2140 1156 1091 829 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7789 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5520 6478 442 Size: 416 53 83 399 24 4103 958 1753 Fragments arranged by size: 4103 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7789 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2843 2843 Size: 7789 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2202 6449 1239 Size: 963 4247 2579 Fragments arranged by size: 4247 2579 963 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2813 3479 3726 4172 4385 5914 6779 7077 2813 Size: 666 247 446 213 1529 865 298 3525 Fragments arranged by size: 3525 1529 865 666 446 298 247 213 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6452 882 Size: 617 4953 2219 Fragments arranged by size: 4953 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2423 5580 493 Size: 711 1219 3157 2702 Fragments arranged by size: 3157 2702 1219 711 BsaBI GATnn'nnATC Cuts at: 7783 7783 Size: 7789 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5596 5978 6613 275 Size: 53 83 186 936 4063 382 635 1451 Fragments arranged by size: 4063 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6222 6620 6894 7041 7452 6222 Size: 398 274 147 411 6559 Fragments arranged by size: 6559 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3269 3299 1178 Size: 30 165 30 1866 30 5668 Fragments arranged by size: 5668 1866 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2144 1186 Size: 958 6831 BseYI C'CCAG_C Cuts at: 2088 6992 7488 2088 Size: 4904 496 2389 Fragments arranged by size: 4904 2389 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 7216 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5239 6000 7028 1540 Size: 275 186 3238 761 1028 2301 Fragments arranged by size: 3238 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5850 5935 7006 5850 Size: 85 1071 6633 Fragments arranged by size: 6633 1071 85 BsmI GAATG_Cn' Cuts at: 2140 5300 5393 2140 Size: 3160 93 4536 Fragments arranged by size: 4536 3160 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2619 3710 3917 5108 5850 5935 7006 Size: 399 829 1091 207 1191 742 85 1071 Cuts at: 7006 1391 Size: 2174 Fragments arranged by size: 2174 1191 1091 1071 829 742 399 207 85 BspEI T'CCGG_A Cuts at: 7452 7452 Size: 7789 BspHI T'CATG_A Cuts at: 4332 5678 7408 4332 Size: 1346 1730 4713 Fragments arranged by size: 4713 1730 1346 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2597 5144 844 Size: 1753 2547 3489 Fragments arranged by size: 3489 2547 1753 BsrBI CCG'CTC Cuts at: 1394 1823 5676 1394 Size: 429 3853 3507 Fragments arranged by size: 3853 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3600 4236 4333 6284 6458 65 Size: 1183 2352 636 97 1951 174 1396 Fragments arranged by size: 2352 1951 1396 1183 636 174 97 BsrFI r'CCGG_y Cuts at: 1700 1810 6433 6620 1700 Size: 110 4623 187 2869 Fragments arranged by size: 4623 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2847 3982 96 Size: 2751 1135 3903 Fragments arranged by size: 3903 2751 1135 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7789 BssSI C'ACGA_G Cuts at: 5849 6861 5849 Size: 1012 6777 BstAPI GCAn_nnn'nTGC Cuts at: 794 2579 794 Size: 1785 6004 BstBI TT'CG_AA Cuts at: 4494 6593 4494 Size: 2099 5690 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7789 BstXI CCAn_nnnn'nTGG Cuts at: 4657 4657 Size: 7789 BstYI r'GATC_y Cuts at: 1972 5119 5486 5871 5888 7340 7426 7438 7784 Size: 3147 367 385 17 1452 86 12 346 Cuts at: 7784 1972 Size: 1977 Fragments arranged by size: 3147 1977 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2415 513 Size: 552 1350 5887 Fragments arranged by size: 5887 1350 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4358 487 Size: 683 56 3132 3918 Fragments arranged by size: 3918 3132 683 56 BtsI GCAGTG_nn' Cuts at: 5305 6099 6119 5305 Size: 794 20 6975 Fragments arranged by size: 6975 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7789 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7754 DraI TTT'AAA Cuts at: 5093 5093 Size: 7789 DraIII CAC_nnn'GTG Cuts at: 2915 4442 2915 Size: 1527 6262 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6796 809 Size: 507 5480 1802 Fragments arranged by size: 5480 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4353 5236 6125 Size: 54 1425 50 275 18 2523 883 889 Cuts at: 6125 7754 8 Size: 1629 43 Fragments arranged by size: 2523 1629 1425 889 883 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5236 1537 Size: 275 3424 4090 Fragments arranged by size: 4090 3424 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2121 2388 4162 4173 5719 1353 Size: 768 267 1774 11 1546 3423 Fragments arranged by size: 3423 1774 1546 768 267 11 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6376 6750 6896 387 Size: 1064 138 4787 374 146 1280 Fragments arranged by size: 4787 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2145 2160 2202 2406 2474 2628 3923 Size: 393 513 15 42 204 68 154 1295 Cuts at: 3923 4812 5852 6449 7236 7504 1239 Size: 889 1040 597 787 268 1524 Fragments arranged by size: 1524 1295 1040 889 787 597 513 393 268 204 154 68 42 15 EcoNI CCTnn'n_nnAGG Cuts at: 3623 3623 Size: 7789 EcoO109I rG'GnC_Cy Cuts at: 3721 3721 Size: 7789 EcoRI G'AATT_C Cuts at: 5098 5476 5098 Size: 378 7411 EcoRV GAT'ATC Cuts at: 4663 4774 4663 Size: 111 7678 FspI TGC'GCA Cuts at: 6295 6295 Size: 7789 HaeII r_GCGC'y Cuts at: 1678 2066 6936 1678 Size: 388 4870 2531 Fragments arranged by size: 4870 2531 388 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2381 2413 2666 2698 6430 Size: 32 135 32 809 32 253 32 3732 Cuts at: 6430 6462 6504 6536 1373 Size: 32 42 32 2626 Fragments arranged by size: 3732 2626 809 253 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5133 5379 669 Size: 988 281 3195 246 3079 Fragments arranged by size: 3195 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 2856 748 Size: 2108 5681 HpaI GTT'AAC Cuts at: 5379 5379 Size: 7789 KpnI G_GTAC'C Cuts at: 4702 5114 4702 Size: 412 7377 MfeI C'AATT_G Cuts at: 2574 3195 5388 2574 Size: 621 2193 4975 Fragments arranged by size: 4975 2193 621 MluI A'CGCG_T Cuts at: 7665 7665 Size: 7789 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4215 6903 7087 1305 Size: 2910 2688 184 2007 Fragments arranged by size: 2910 2688 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4355 10 Size: 54 1425 343 2523 3444 Fragments arranged by size: 3444 2523 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3293 3543 4319 5747 6106 6265 518 Size: 648 2127 250 776 1428 359 159 2042 Fragments arranged by size: 2127 2042 1428 776 648 359 250 159 MspA1I CmG'CkG Cuts at: 1606 4391 5205 5882 7030 7275 1606 Size: 2785 814 677 1148 245 2120 Fragments arranged by size: 2785 2120 1148 814 677 245 NcoI C'CATG_G Cuts at: 513 1065 2415 513 Size: 552 1350 5887 Fragments arranged by size: 5887 1350 552 NdeI CA'TA_TG Cuts at: 387 2653 3897 387 Size: 2266 1244 4279 Fragments arranged by size: 4279 2266 1244 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7789 NotI GC'GGCC_GC Cuts at: 5236 5236 Size: 7789 NsiI A_TGCA'T Cuts at: 4595 4595 Size: 7789 NspI r_CATG'y Cuts at: 1132 1229 4039 4136 4505 5147 6692 1132 Size: 97 2810 97 369 642 1545 2229 Fragments arranged by size: 2810 2229 1545 642 369 97 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7789 PciI A'CATG_T Cuts at: 4035 4501 6688 4035 Size: 466 2187 5136 Fragments arranged by size: 5136 2187 466 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7789 PmeI GTTT'AAAC Cuts at: 5093 5093 Size: 7789 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3269 3301 5858 5890 7371 7403 3269 Size: 32 2557 32 1481 32 3655 Fragments arranged by size: 3655 2557 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3721 3721 Size: 7789 PshAI GACnn'nnGTC Cuts at: 1613 3846 1613 Size: 2233 5556 PsiI TTA'TAA Cuts at: 3371 4148 4872 4934 5359 3371 Size: 777 724 62 425 5801 Fragments arranged by size: 5801 777 724 425 62 PspOMI G'GGCC_C Cuts at: 2615 2615 Size: 7789 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4488 4520 4488 Size: 32 7757 PstI C_TGCA'G Cuts at: 830 2734 5141 830 Size: 1904 2407 3478 Fragments arranged by size: 3478 2407 1904 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7789 PvuII CAG'CTG Cuts at: 4391 4391 Size: 7789 SalI G'TCGA_C Cuts at: 1936 5131 1936 Size: 3195 4594 SbfI CC_TGCA'GG Cuts at: 5141 5141 Size: 7789 ScaI AGT'ACT Cuts at: 2826 4926 6037 7749 2826 Size: 2100 1111 1712 2866 Fragments arranged by size: 2866 2100 1712 1111 SfcI C'TryA_G Cuts at: 826 1045 2730 3124 3672 5016 5137 6272 6953 Size: 219 1685 394 548 1344 121 1135 681 Cuts at: 6953 7144 826 Size: 191 1471 Fragments arranged by size: 1685 1471 1344 1135 681 548 394 219 191 121 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7789 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7789 SmaI CCC'GGG Cuts at: 1872 5116 1872 Size: 3244 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2537 2828 3494 3741 4187 4400 Size: 197 926 594 291 666 247 446 213 Cuts at: 4400 5893 6794 7056 820 Size: 1493 901 262 1553 Fragments arranged by size: 1553 1493 926 901 666 594 446 291 262 247 213 197 SnaBI TAC'GTA Cuts at: 493 5580 493 Size: 5087 2702 SpeI A'CTAG_T Cuts at: 152 4927 152 Size: 4775 3014 SphI G_CATG'C Cuts at: 1132 5147 1132 Size: 4015 3774 SspI AAT'ATT Cuts at: 5 52 4382 5713 5 Size: 47 4330 1331 2081 Fragments arranged by size: 4330 2081 1331 47 StuI AGG'CCT Cuts at: 1517 2406 1517 Size: 889 6900 StyI C'CwwG_G Cuts at: 513 1065 2401 2415 3580 4948 5228 513 Size: 552 1336 14 1165 1368 280 3074 Fragments arranged by size: 3074 1368 1336 1165 552 280 14 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5816 5833 5986 6171 6616 325 Size: 1255 4236 17 153 185 445 1498 Fragments arranged by size: 4236 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2770 2824 2847 Size: 274 80 33 51 1443 793 54 23 Cuts at: 2847 3982 4504 4924 6035 7747 96 Size: 1135 522 420 1111 1712 138 Fragments arranged by size: 1712 1443 1135 1111 793 522 420 274 138 80 54 51 33 23 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7749 7781 7749 Size: 32 7757 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7789 XbaI T'CTAG_A Cuts at: 5125 5125 Size: 7789 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3607 3607 Size: 7789 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7789 XmaI C'CCGG_G Cuts at: 1870 5114 1870 Size: 3244 4545 XmnI GAAnn'nnTTC Cuts at: 2012 2345 2508 4072 4182 4737 5918 2012 Size: 333 163 1564 110 555 1181 3883 Fragments arranged by size: 3883 1564 1181 555 333 163 110 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5597 276 Size: 53 83 186 936 4063 2468 Fragments arranged by size: 4063 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI BaeI BbeI BbvCI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI