(Circular) (Six-base) MAPSORT of: pFN21AA1119.seq Check: 3871 from: 1 to: 10252 pFN21AA1119 10252 bp With 182 enzymes: SgfI * January 31, 2011 17:48 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3181 4440 5386 6674 3181 Size: 1259 946 1288 6759 Fragments arranged by size: 6759 1288 1259 946 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6647 7116 8062 278 Size: 53 83 186 936 5111 469 946 2468 Fragments arranged by size: 5111 2468 946 936 469 186 83 53 AccI GT'mk_AC Cuts at: 1937 3072 7595 1937 Size: 1135 4523 4594 Fragments arranged by size: 4594 4523 1135 Acc65I G'GTAC_C Cuts at: 2697 5205 7573 2697 Size: 2508 2368 5376 Fragments arranged by size: 5376 2508 2368 AclI AA'CG_TT Cuts at: 1563 8379 8752 1563 Size: 6816 373 3063 Fragments arranged by size: 6816 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3764 4385 4733 4910 5468 5627 5663 6131 Size: 2132 621 348 177 558 159 36 468 Cuts at: 6131 6308 6707 8315 9699 9967 1632 Size: 177 399 1608 1384 268 1917 Fragments arranged by size: 2132 1917 1608 1384 621 558 468 399 348 268 177 177 159 36 AfeI AGC'GCT Cuts at: 6929 6929 Size: 10252 AflII C'TTAA_G Cuts at: 820 1017 6752 820 Size: 197 5735 4320 Fragments arranged by size: 5735 4320 197 AflIII A'CryG_T Cuts at: 1203 6525 7455 9151 10128 1203 Size: 5322 930 1696 977 1327 Fragments arranged by size: 5322 1696 1327 977 930 AgeI A'CCGG_T Cuts at: 4119 9083 4119 Size: 4964 5288 AhdI GACnn_n'nnGTC Cuts at: 2369 8981 2369 Size: 6612 3640 AleI CACnn'nnGTG Cuts at: 1166 7197 1166 Size: 6031 4221 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2195 3246 4994 5091 7151 1184 Size: 1011 1051 1748 97 2060 4285 Fragments arranged by size: 4285 2060 1748 1051 1011 97 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7212 7244 7212 Size: 32 10220 AlwNI CAG_nnn'CTG Cuts at: 4044 4651 6061 6484 6787 7246 7435 7995 9567 Size: 607 1410 423 303 459 189 560 1572 Cuts at: 9567 4044 Size: 4729 Fragments arranged by size: 4729 1572 1410 607 560 459 423 303 189 ApaI G_GGCC'C Cuts at: 4489 4990 4489 Size: 501 9751 ApaLI G'TGCA_C Cuts at: 2032 4904 6569 8309 9465 2032 Size: 2872 1665 1740 1156 2819 Fragments arranged by size: 2872 2819 1740 1665 1156 ApoI r'AATT_y Cuts at: 1493 2822 5138 6182 6757 7329 7358 7535 7561 Size: 1329 2316 1044 575 572 29 177 26 Cuts at: 7561 7787 7939 10048 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2316 2109 1697 1329 1044 575 572 226 177 152 29 26 AseI AT'TA_AT Cuts at: 160 1032 2334 5862 8806 160 Size: 872 1302 3528 2944 1606 Fragments arranged by size: 3528 2944 1606 1302 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 10252 AvaI C'yCGr_G Cuts at: 1870 1943 4542 5768 6101 7577 1870 Size: 73 2599 1226 333 1476 4545 Fragments arranged by size: 4545 2599 1476 1226 333 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2299 2332 2299 Size: 33 10219 BamHI G'GATC_C Cuts at: 2046 7582 7949 2046 Size: 5536 367 4349 Fragments arranged by size: 5536 4349 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2697 5205 7573 9028 618 Size: 325 217 627 910 2508 2368 1455 1842 Fragments arranged by size: 2508 2368 1842 1455 910 627 325 217 BanII G_rGCy'C Cuts at: 1391 2022 3580 4152 4489 4922 4990 5769 6366 Size: 631 1558 572 337 433 68 779 597 Cuts at: 6366 7571 1391 Size: 1205 4072 Fragments arranged by size: 4072 1558 1205 779 631 597 572 433 337 68 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2516 2746 2807 2930 5434 928 Size: 971 617 230 61 123 2504 5746 Fragments arranged by size: 5746 2504 971 617 230 123 61 BbvCI CC'TCA_GC Cuts at: 2910 5042 5663 7307 7428 2910 Size: 2132 621 1644 121 5734 Fragments arranged by size: 5734 2132 1644 621 121 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 8443 8477 1903 Size: 34 6506 34 3678 Fragments arranged by size: 6506 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 8144 9354 8144 Size: 1210 9042 BclI T'GATC_A Cuts at: 1548 3195 7071 1548 Size: 1647 3876 4729 Fragments arranged by size: 4729 3876 1647 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2664 4493 5921 Size: 107 122 71 631 605 992 1829 1428 Cuts at: 5921 8863 136 Size: 2942 1525 Fragments arranged by size: 2942 1829 1525 1428 992 631 605 122 107 71 BglII A'GATC_T Cuts at: 4239 5004 5587 6807 10247 4239 Size: 765 583 1220 3440 4244 Fragments arranged by size: 4244 3440 1220 765 583 BlpI GC'TnA_GC Cuts at: 2074 3030 4425 4738 6042 6096 7669 2074 Size: 956 1395 313 1304 54 1573 4657 Fragments arranged by size: 4657 1573 1395 1304 956 313 54 Bme1580I G_kGCm'C Cuts at: 1790 2036 4489 4908 4990 6494 6501 6573 8313 Size: 246 2453 419 82 1504 7 72 1740 Cuts at: 8313 9469 1790 Size: 1156 2573 Fragments arranged by size: 2573 2453 1740 1504 1156 419 246 82 72 7 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 10252 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3229 3583 7983 Size: 416 53 83 399 24 1812 354 4400 Cuts at: 7983 8941 442 Size: 958 1753 Fragments arranged by size: 4400 1812 1753 958 416 399 354 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 10252 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3700 4753 3700 Size: 1053 9199 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2150 2306 2520 3374 3443 3586 3719 4211 Size: 911 156 214 854 69 143 133 492 Cuts at: 4211 4292 4445 5084 5207 5274 5375 5435 5495 Size: 81 153 639 123 67 101 60 60 Cuts at: 5495 5528 5642 5686 6056 6083 6437 6919 8912 Size: 33 114 44 370 27 354 482 1993 Cuts at: 8912 1239 Size: 2579 Fragments arranged by size: 2579 1993 911 854 639 492 482 370 354 214 156 153 143 133 123 114 101 81 69 67 60 60 44 33 27 Bpu10I CC'TnA_GC Cuts at: 2234 2910 3077 5042 5663 7307 7428 2234 Size: 676 167 1965 621 1644 121 5058 Fragments arranged by size: 5058 1965 1644 676 621 167 121 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3272 4679 5732 6074 6548 7184 8377 9242 9540 Size: 1407 1053 342 474 636 1193 865 298 Cuts at: 9540 3272 Size: 3984 Fragments arranged by size: 3984 1407 1193 1053 865 636 474 342 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3155 3932 8915 882 Size: 617 1656 777 4983 2219 Fragments arranged by size: 4983 2219 1656 777 617 BsaAI yAC'GTr Cuts at: 493 1204 4615 8043 493 Size: 711 3411 3428 2702 Fragments arranged by size: 3428 3411 2702 711 BsaBI GATnn'nnATC Cuts at: 3737 4067 10246 3737 Size: 330 6179 3743 Fragments arranged by size: 6179 3743 330 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5016 6644 7113 8059 Size: 53 83 186 936 3483 1628 469 946 Cuts at: 8059 8441 9076 275 Size: 382 635 1451 Fragments arranged by size: 3483 1628 1451 946 936 635 469 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4119 4296 4686 6450 6921 8685 9083 9357 9504 Size: 177 390 1764 471 1764 398 274 147 Cuts at: 9504 9915 4119 Size: 411 4456 Fragments arranged by size: 4456 1764 1764 471 411 398 390 274 177 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3584 3614 1178 Size: 30 165 30 2181 30 7816 Fragments arranged by size: 7816 2181 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2892 2936 4967 5159 5204 5207 5777 5798 Size: 1706 44 2031 192 45 3 570 21 Cuts at: 5798 6086 6089 6131 6434 6922 7181 1186 Size: 288 3 42 303 488 259 4257 Fragments arranged by size: 4257 2031 1706 570 488 303 288 259 192 45 44 42 21 3 3 BseYI C'CCAG_C Cuts at: 3514 7239 7314 7516 9455 9951 3514 Size: 3725 75 202 1939 496 3815 Fragments arranged by size: 3815 3725 1939 496 202 75 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2868 3260 4049 4095 5522 1163 Size: 573 1132 392 789 46 1427 5893 Fragments arranged by size: 5893 1427 1132 789 573 392 46 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 7702 8463 9491 1540 Size: 275 186 5701 761 1028 2301 Fragments arranged by size: 5701 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2022 2036 3111 3126 3261 3888 4152 4431 4461 Size: 14 1075 15 135 627 264 279 30 Cuts at: 4461 4590 4908 5769 5793 6366 6573 7130 8313 Size: 129 318 861 24 573 207 557 1183 Cuts at: 8313 8398 9469 2022 Size: 85 1071 2805 Fragments arranged by size: 2805 1183 1075 1071 861 627 573 557 318 279 264 207 135 129 85 30 24 15 14 BsmI GAATG_Cn' Cuts at: 3952 5587 6885 6997 7763 7856 3952 Size: 1635 1298 112 766 93 6348 Fragments arranged by size: 6348 1635 1298 766 112 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4052 4763 4052 Size: 711 9541 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2022 2036 3111 3126 3261 3580 3888 Size: 399 232 14 1075 15 135 319 308 Cuts at: 3888 4152 4431 4461 4489 4590 4908 4922 4990 Size: 264 279 30 28 101 318 14 68 Cuts at: 4990 5769 5793 6366 6494 6501 6573 7130 7571 Size: 779 24 573 128 7 72 557 441 Cuts at: 7571 8313 8398 9469 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1075 1071 779 742 573 557 441 399 319 318 308 279 264 232 135 128 101 85 72 68 30 28 24 15 14 14 7 BspEI T'CCGG_A Cuts at: 4296 4686 6450 9915 4296 Size: 390 1764 3465 4633 Fragments arranged by size: 4633 3465 1764 390 BspHI T'CATG_A Cuts at: 2245 3781 4311 5172 6714 8141 9871 2245 Size: 1536 530 861 1542 1427 1730 2626 Fragments arranged by size: 2626 1730 1542 1536 1427 861 530 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3181 4170 4440 5386 6364 6674 7036 7104 Size: 2337 989 270 946 978 310 362 68 Cuts at: 7104 7607 844 Size: 503 3489 Fragments arranged by size: 3489 2337 989 978 946 503 362 310 270 68 BsrBI CCG'CTC Cuts at: 1394 1823 4448 4737 4885 7417 8139 1394 Size: 429 2625 289 148 2532 722 3507 Fragments arranged by size: 3507 2625 2532 722 429 289 148 BsrDI GCAATG_nn' Cuts at: 65 1248 2650 8747 8921 65 Size: 1183 1402 6097 174 1396 Fragments arranged by size: 6097 1402 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2810 3594 4119 5837 6914 8896 9083 Size: 110 1000 784 525 1718 1077 1982 187 Cuts at: 9083 1700 Size: 2869 Fragments arranged by size: 2869 1982 1718 1077 1000 784 525 187 110 BsrGI T'GTAC_A Cuts at: 96 2164 96 Size: 2068 8184 BssHII G'CGCG_C Cuts at: 1925 3203 1925 Size: 1278 8974 BssSI C'ACGA_G Cuts at: 5759 8312 9324 5759 Size: 2553 1012 6687 Fragments arranged by size: 6687 2553 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3087 6577 794 Size: 2293 3490 4469 Fragments arranged by size: 4469 3490 2293 BstBI TT'CG_AA Cuts at: 3833 4066 9056 3833 Size: 233 4990 5029 Fragments arranged by size: 5029 4990 233 BstEII G'GTnAC_C Cuts at: 1184 3148 1184 Size: 1964 8288 BstXI CCAn_nnnn'nTGG Cuts at: 2011 4095 6034 2011 Size: 2084 1939 6229 Fragments arranged by size: 6229 2084 1939 BstYI r'GATC_y Cuts at: 1972 2046 3450 4239 5004 5587 6807 7582 7949 Size: 74 1404 789 765 583 1220 775 367 Cuts at: 7949 8334 8351 9803 9889 9901 10247 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1404 1220 789 775 765 583 385 367 346 86 74 17 12 BstZ17I GTA'TAC Cuts at: 3073 3073 Size: 10252 Bsu36I CC'TnA_GG Cuts at: 4721 4940 5798 4721 Size: 219 858 9175 Fragments arranged by size: 9175 858 219 BtgI C'CryG_G Cuts at: 513 1065 4872 5427 5673 5707 7215 7497 513 Size: 552 3807 555 246 34 1508 282 3268 Fragments arranged by size: 3807 3268 1508 555 552 282 246 34 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3122 487 Size: 683 56 1896 7617 Fragments arranged by size: 7617 1896 683 56 BtsI GCAGTG_nn' Cuts at: 4032 6726 7086 7768 8562 8582 4032 Size: 2694 360 682 794 20 5702 Fragments arranged by size: 5702 2694 794 682 360 20 ClaI AT'CG_AT Cuts at: 1554 4806 1554 Size: 3252 7000 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 10217 DraI TTT'AAA Cuts at: 2827 5976 7335 7556 2827 Size: 3149 1359 221 5523 Fragments arranged by size: 5523 3149 1359 221 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5438 9259 809 Size: 507 4122 3821 1802 Fragments arranged by size: 4122 3821 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2389 2454 2562 Size: 54 1425 50 275 18 559 65 108 Cuts at: 2562 3901 4228 5676 7699 8588 10217 8 Size: 1339 327 1448 2023 889 1629 43 Fragments arranged by size: 2023 1629 1448 1425 1339 889 559 327 275 108 65 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 7699 1537 Size: 275 5887 4090 Fragments arranged by size: 5887 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2577 3720 4136 5453 5507 5603 7067 8182 Size: 1224 1143 416 1317 54 96 1464 1115 Cuts at: 8182 1353 Size: 3423 Fragments arranged by size: 3423 1464 1317 1224 1143 1115 416 96 54 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5317 5561 6971 8839 9213 9359 Size: 1064 138 3728 244 1410 1868 374 146 Cuts at: 9359 387 Size: 1280 Fragments arranged by size: 3728 1868 1410 1280 1064 374 244 146 138 EcoICRI GAG'CTC Cuts at: 2020 4150 5767 6364 2020 Size: 2130 1617 597 5908 Fragments arranged by size: 5908 2130 1617 597 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2150 2306 2520 3374 3443 3586 3719 Size: 393 518 156 214 854 69 143 133 Cuts at: 3719 3764 4211 4292 4385 4445 4733 4910 5084 Size: 45 447 81 93 60 288 177 174 Cuts at: 5084 5207 5274 5375 5435 5468 5495 5528 5627 Size: 123 67 101 60 33 27 33 99 Cuts at: 5627 5642 5663 5686 6056 6083 6131 6308 6437 Size: 15 21 23 370 27 48 177 129 Cuts at: 6437 6707 6919 8315 8912 9699 9967 1239 Size: 270 212 1396 597 787 268 1524 Fragments arranged by size: 1524 1396 854 787 597 518 447 393 370 288 270 268 214 212 177 177 174 156 143 133 129 123 101 99 93 81 69 67 60 60 48 45 33 33 27 27 23 21 15 EcoNI CCTnn'n_nnAGG Cuts at: 3188 4202 4501 5219 5393 5417 6113 3188 Size: 1014 299 718 174 24 696 7327 Fragments arranged by size: 7327 1014 718 696 299 174 24 EcoO109I rG'GnC_Cy Cuts at: 3271 3310 3550 3793 4207 4485 4576 4717 4986 Size: 39 240 243 414 278 91 141 269 Cuts at: 4986 5802 6106 6313 6439 3271 Size: 816 304 207 126 7084 Fragments arranged by size: 7084 816 414 304 278 269 243 240 207 141 126 91 39 EcoRI G'AATT_C Cuts at: 6182 7535 7561 7939 6182 Size: 1353 26 378 8495 Fragments arranged by size: 8495 1353 378 26 EcoRV GAT'ATC Cuts at: 2203 2203 Size: 10252 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4018 4261 4018 Size: 243 10009 FspI TGC'GCA Cuts at: 4970 5777 8758 4970 Size: 807 2981 6464 Fragments arranged by size: 6464 2981 807 HaeII r_GCGC'y Cuts at: 1678 2074 5820 6358 6931 9399 1678 Size: 396 3746 538 573 2468 2531 Fragments arranged by size: 3746 2531 2468 573 538 396 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2355 2387 3584 3616 4832 Size: 32 135 32 783 32 1197 32 1216 Cuts at: 4832 4864 5183 5215 5609 5641 6629 6661 7103 Size: 32 319 32 394 32 988 32 442 Cuts at: 7103 7135 8893 8925 8967 8999 1373 Size: 32 1758 32 42 32 2626 Fragments arranged by size: 2626 1758 1216 1197 988 783 442 394 319 135 42 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2085 5902 7596 7842 669 Size: 988 281 147 3817 1694 246 3079 Fragments arranged by size: 3817 3079 1694 988 281 246 147 HindIII A'AGCT_T Cuts at: 748 748 Size: 10252 HpaI GTT'AAC Cuts at: 2085 7842 2085 Size: 5757 4495 KpnI G_GTAC'C Cuts at: 2701 5209 7577 2701 Size: 2508 2368 5376 Fragments arranged by size: 5376 2508 2368 MfeI C'AATT_G Cuts at: 2157 7851 2157 Size: 5694 4558 MluI A'CGCG_T Cuts at: 10128 10128 Size: 10252 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2316 3513 3660 3900 4035 5217 6684 6852 Size: 1011 1197 147 240 135 1182 1467 168 Cuts at: 6852 9366 9550 1305 Size: 2514 184 2007 Fragments arranged by size: 2514 2007 1467 1197 1182 1011 240 184 168 147 135 MscI TGG'CCA Cuts at: 10 64 1489 1832 2391 2456 3903 10 Size: 54 1425 343 559 65 1447 6359 Fragments arranged by size: 6359 1447 1425 559 343 65 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 3228 4093 4310 4553 5432 6627 Size: 648 843 1219 865 217 243 879 1195 Cuts at: 6627 7133 7197 8210 8569 8728 518 Size: 506 64 1013 359 159 2042 Fragments arranged by size: 2042 1219 1195 1013 879 865 843 648 506 359 243 217 159 64 MspA1I CmG'CkG Cuts at: 1606 2078 2179 3972 4079 4648 4792 4798 5506 Size: 472 101 1793 107 569 144 6 708 Cuts at: 5506 5827 6046 6388 6699 7668 8345 9493 9738 Size: 321 219 342 311 969 677 1148 245 Cuts at: 9738 1606 Size: 2120 Fragments arranged by size: 2120 1793 1148 969 708 677 569 472 342 321 311 245 219 144 107 101 6 NaeI GCC'GGC Cuts at: 5839 5839 Size: 10252 NcoI C'CATG_G Cuts at: 513 1065 4872 5427 7215 7497 513 Size: 552 3807 555 1788 282 3268 Fragments arranged by size: 3807 3268 1788 555 552 282 NdeI CA'TA_TG Cuts at: 387 387 Size: 10252 NgoMIV G'CCGG_C Cuts at: 5837 5837 Size: 10252 NheI G'CTAG_C Cuts at: 1052 1052 Size: 10252 NotI GC'GGCC_GC Cuts at: 7699 7699 Size: 10252 NspI r_CATG'y Cuts at: 1132 1229 3413 3634 4480 5900 6415 6529 7138 Size: 97 2184 221 846 1420 515 114 609 Cuts at: 7138 7459 7610 9155 1132 Size: 321 151 1545 2229 Fragments arranged by size: 2229 2184 1545 1420 846 609 515 321 221 151 114 97 PasI CC'CwG_GG Cuts at: 1348 3815 1348 Size: 2467 7785 PciI A'CATG_T Cuts at: 6525 7455 9151 6525 Size: 930 1696 7626 Fragments arranged by size: 7626 1696 930 PflMI CCAn_nnn'nTGG Cuts at: 1108 2360 2398 5398 6061 6334 7024 1108 Size: 1252 38 3000 663 273 690 4336 Fragments arranged by size: 4336 3000 1252 690 663 273 38 PfoI T'CCnGG_A Cuts at: 2283 3312 4188 2283 Size: 1029 876 8347 Fragments arranged by size: 8347 1029 876 PmeI GTTT'AAAC Cuts at: 7556 7556 Size: 10252 PmlI CAC'GTG Cuts at: 4615 4615 Size: 10252 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 8321 8353 9834 9866 8321 Size: 32 1481 32 8707 Fragments arranged by size: 8707 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3310 3550 3793 4207 4576 5802 6313 3310 Size: 240 243 414 369 1226 511 7249 Fragments arranged by size: 7249 1226 511 414 369 243 240 PshAI GACnn'nnGTC Cuts at: 1613 3167 1613 Size: 1554 8698 PsiI TTA'TAA Cuts at: 7822 7822 Size: 10252 PspOMI G'GGCC_C Cuts at: 4485 4986 4485 Size: 501 9751 PspXI vC'TCGA_Gb Cuts at: 5768 6101 5768 Size: 333 9919 PstI C_TGCA'G Cuts at: 830 2257 3189 4181 4344 4389 4797 4950 4983 Size: 1427 932 992 163 45 408 153 33 Cuts at: 4983 5457 6051 7047 7604 830 Size: 474 594 996 557 3478 Fragments arranged by size: 3478 1427 996 992 932 594 557 474 408 163 153 45 33 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 10252 PvuII CAG'CTG Cuts at: 2078 2179 4079 4792 5506 6046 2078 Size: 101 1900 713 714 540 6284 Fragments arranged by size: 6284 1900 714 713 540 101 SacI G_AGCT'C Cuts at: 2022 4152 5769 6366 2022 Size: 2130 1617 597 5908 Fragments arranged by size: 5908 2130 1617 597 SalI G'TCGA_C Cuts at: 1936 7594 1936 Size: 5658 4594 SapI GCTCTTCn'nnn_ Cuts at: 3720 7067 3720 Size: 3347 6905 SbfI CC_TGCA'GG Cuts at: 7047 7604 7047 Size: 557 9695 ScaI AGT'ACT Cuts at: 8500 10212 8500 Size: 1712 8540 SexAI A'CCwGG_T Cuts at: 6456 6456 Size: 10252 SfcI C'TryA_G Cuts at: 826 1045 2023 2117 2253 2383 3049 3185 4177 Size: 219 978 94 136 130 666 136 992 Cuts at: 4177 4340 4385 4793 4946 4979 5453 5725 6047 Size: 163 45 408 153 33 474 272 322 Cuts at: 6047 7043 7600 8735 9416 9607 826 Size: 996 557 1135 681 191 1471 Fragments arranged by size: 1471 1135 996 992 978 681 666 557 474 408 322 272 219 191 163 153 136 136 130 94 45 33 SfiI GGCCn_nnn'nGGCC Cuts at: 4493 4493 Size: 10252 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 10252 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 10252 SmaI CCC'GGG Cuts at: 1872 4544 7579 1872 Size: 2672 3035 4545 Fragments arranged by size: 4545 3035 2672 SmlI C'TyrA_G Cuts at: 820 1017 1943 3287 4694 5747 5768 6089 6101 Size: 197 926 1344 1407 1053 21 321 12 Cuts at: 6101 6563 6752 7163 8356 9257 9519 820 Size: 462 189 411 1193 901 262 1553 Fragments arranged by size: 1553 1407 1344 1193 1053 926 901 462 411 321 262 197 189 21 12 SnaBI TAC'GTA Cuts at: 493 8043 493 Size: 7550 2702 SpeI A'CTAG_T Cuts at: 152 3763 152 Size: 3611 6641 SphI G_CATG'C Cuts at: 1132 4480 6415 7138 7610 1132 Size: 3348 1935 723 472 3774 Fragments arranged by size: 3774 3348 1935 723 472 SspI AAT'ATT Cuts at: 5 52 5228 8176 5 Size: 47 5176 2948 2081 Fragments arranged by size: 5176 2948 2081 47 StuI AGG'CCT Cuts at: 1517 4496 4535 5924 6012 1517 Size: 2979 39 1389 88 5757 Fragments arranged by size: 5757 2979 1389 88 39 StyI C'CwwG_G Cuts at: 513 1065 2088 3007 3455 4872 5427 6007 7215 Size: 552 1023 919 448 1417 555 580 1208 Cuts at: 7215 7497 7691 513 Size: 282 194 3074 Fragments arranged by size: 3074 1417 1208 1023 919 580 555 552 448 282 194 SwaI ATTT'AAAT Cuts at: 5976 5976 Size: 10252 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 7328 8279 8296 8449 8634 9079 325 Size: 1255 5748 951 17 153 185 445 1498 Fragments arranged by size: 5748 1498 1255 951 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2164 2670 6693 Size: 274 80 33 51 1443 187 506 4023 Cuts at: 6693 8498 10210 96 Size: 1805 1712 138 Fragments arranged by size: 4023 1805 1712 1443 506 274 187 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 10212 10244 10212 Size: 32 10220 Tth111I GACn'n_nGTC Cuts at: 1316 3178 1316 Size: 1862 8390 XbaI T'CTAG_A Cuts at: 7588 7588 Size: 10252 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2608 2896 6223 2608 Size: 288 3327 6637 Fragments arranged by size: 6637 3327 288 XhoI C'TCGA_G Cuts at: 1943 5768 6101 1943 Size: 3825 333 6094 Fragments arranged by size: 6094 3825 333 XmaI C'CCGG_G Cuts at: 1870 4542 7577 1870 Size: 2672 3035 4545 Fragments arranged by size: 4545 3035 2672 XmnI GAAnn'nnTTC Cuts at: 2142 6401 8381 2142 Size: 4259 1980 4013 Fragments arranged by size: 4259 4013 1980 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6645 7114 8060 276 Size: 53 83 186 936 5111 469 946 2468 Fragments arranged by size: 5111 2468 946 936 469 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BbeI BfrBI BsiWI DraIII FseI FspAI KasI NarI NruI NsiI PacI PsrI RsrII SacII SanDI SfoI SrfI