(Circular) (Six-base) MAPSORT of: pFN21AA1115.seq Check: 93 from: 1 to: 7348 pFN21AA1115 7348 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2172 2172 Size: 7348 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3341 5158 278 Size: 53 83 186 936 1805 1817 2468 Fragments arranged by size: 2468 1817 1805 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4691 1937 Size: 2754 4594 Acc65I G'GTAC_C Cuts at: 4669 4669 Size: 7348 AclI AA'CG_TT Cuts at: 1563 5475 5848 1563 Size: 3912 373 3063 Fragments arranged by size: 3912 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2359 2863 3497 4400 5411 6795 7063 1632 Size: 727 504 634 903 1011 1384 268 1917 Fragments arranged by size: 1917 1384 1011 903 727 634 504 268 AflII C'TTAA_G Cuts at: 820 1017 2758 820 Size: 197 1741 5410 Fragments arranged by size: 5410 1741 197 AflIII A'CryG_T Cuts at: 1203 2766 6247 7224 1203 Size: 1563 3481 977 1327 Fragments arranged by size: 3481 1563 1327 977 AgeI A'CCGG_T Cuts at: 6179 6179 Size: 7348 AhdI GACnn_n'nnGTC Cuts at: 6077 6077 Size: 7348 AleI CACnn'nnGTG Cuts at: 1166 2519 1166 Size: 1353 5995 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7348 AlwNI CAG_nnn'CTG Cuts at: 2587 2647 2708 2818 2995 3475 4273 5091 6663 Size: 60 61 110 177 480 798 818 1572 Cuts at: 6663 2587 Size: 3272 Fragments arranged by size: 3272 1572 818 798 480 177 110 61 60 ApaI G_GGCC'C Cuts at: 2923 3450 3531 3967 4239 4507 2923 Size: 527 81 436 272 268 5764 Fragments arranged by size: 5764 527 436 272 268 81 ApaLI G'TGCA_C Cuts at: 5405 6561 5405 Size: 1156 6192 ApoI r'AATT_y Cuts at: 1493 4657 4883 5035 7144 1493 Size: 3164 226 152 2109 1697 Fragments arranged by size: 3164 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5902 160 Size: 872 4870 1606 Fragments arranged by size: 4870 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7348 AvaI C'yCGr_G Cuts at: 1870 1943 2078 4185 4673 1870 Size: 73 135 2107 488 4545 Fragments arranged by size: 4545 2107 488 135 73 BamHI G'GATC_C Cuts at: 2808 3330 4434 4678 5045 2808 Size: 522 1104 244 367 5111 Fragments arranged by size: 5111 1104 522 367 244 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2294 2477 2948 3419 3932 Size: 325 217 627 507 183 471 471 513 Cuts at: 3932 4669 6124 618 Size: 737 1455 1842 Fragments arranged by size: 1842 1455 737 627 513 507 471 471 325 217 183 BanII G_rGCy'C Cuts at: 1391 2838 2907 2923 3135 3450 3531 3908 3967 Size: 1447 69 16 212 315 81 377 59 Cuts at: 3967 4239 4377 4507 4544 4551 4667 1391 Size: 272 138 130 37 7 116 4072 Fragments arranged by size: 4072 1447 377 315 272 212 138 130 116 81 69 59 37 16 7 BbeI G_GCGC'C Cuts at: 2952 3936 2952 Size: 984 6364 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2103 3178 4056 928 Size: 971 204 1075 878 4220 Fragments arranged by size: 4220 1075 971 878 204 BbvCI CC'TCA_GC Cuts at: 2532 2537 4119 4333 4378 2532 Size: 5 1582 214 45 5502 Fragments arranged by size: 5502 1582 214 45 5 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2752 2786 5539 5573 1903 Size: 34 815 34 2753 34 3678 Fragments arranged by size: 3678 2753 815 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5240 6450 5240 Size: 1210 6138 BclI T'GATC_A Cuts at: 1548 1548 Size: 7348 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2842 2918 3962 Size: 107 122 71 631 605 1170 76 1044 Cuts at: 3962 4502 4555 5959 136 Size: 540 53 1404 1525 Fragments arranged by size: 1525 1404 1170 1044 631 605 540 122 107 76 71 53 BglII A'GATC_T Cuts at: 7343 7343 Size: 7348 BlpI GC'TnA_GC Cuts at: 2975 3236 4765 2975 Size: 261 1529 5558 Fragments arranged by size: 5558 1529 261 Bme1580I G_kGCm'C Cuts at: 1790 2274 2923 3100 3153 3424 3450 3531 3967 Size: 484 649 177 53 271 26 81 436 Cuts at: 3967 4239 4251 4507 4579 5409 6565 1790 Size: 272 12 256 72 830 1156 2573 Fragments arranged by size: 2573 1156 830 649 484 436 272 271 256 177 81 72 53 26 12 BmgBI CAC'GTC Cuts at: 1318 2101 3148 1318 Size: 783 1047 5518 Fragments arranged by size: 5518 1047 783 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2232 2635 4151 Size: 416 53 83 399 24 815 403 1516 Cuts at: 4151 4224 5079 6037 442 Size: 73 855 958 1753 Fragments arranged by size: 1753 1516 958 855 815 416 403 399 83 73 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7348 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2075 2750 2852 2921 3020 3035 3577 4110 Size: 836 675 102 69 99 15 542 533 Cuts at: 4110 4206 6008 1239 Size: 96 1802 2579 Fragments arranged by size: 2579 1802 836 675 542 533 102 99 96 69 15 Bpu10I CC'TnA_GC Cuts at: 2069 2532 2537 2666 3101 3907 4119 4307 4333 Size: 463 5 129 435 806 212 188 26 Cuts at: 4333 4378 2069 Size: 45 5039 Fragments arranged by size: 5039 806 463 435 212 188 129 45 26 5 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3602 3851 5473 6338 6636 3602 Size: 249 1622 865 298 4314 Fragments arranged by size: 4314 1622 865 298 249 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3533 4317 4467 6011 882 Size: 617 2034 784 150 1544 2219 Fragments arranged by size: 2219 2034 1544 784 617 150 BsaAI yAC'GTr Cuts at: 493 1204 3076 5139 493 Size: 711 1872 2063 2702 Fragments arranged by size: 2702 2063 1872 711 BsaBI GATnn'nnATC Cuts at: 2619 7342 2619 Size: 4723 2625 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2949 3272 3338 3933 Size: 53 83 186 936 1416 323 66 595 Cuts at: 3933 4296 5155 5537 6172 275 Size: 363 859 382 635 1451 Fragments arranged by size: 1451 1416 936 859 635 595 382 363 323 186 83 66 53 BsaWI w'CCGG_w Cuts at: 3990 5781 6179 6453 6600 7011 3990 Size: 1791 398 274 147 411 4327 Fragments arranged by size: 4327 1791 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3572 3602 4204 4234 4579 Size: 30 165 30 2169 30 602 30 345 Cuts at: 4579 4609 1178 Size: 30 3917 Fragments arranged by size: 3917 2169 602 345 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2228 2582 3362 3580 3653 3740 3758 3896 Size: 1042 354 780 218 73 87 18 138 Cuts at: 3896 4043 4052 4055 4058 4070 4444 4584 1186 Size: 147 9 3 3 12 374 140 3950 Fragments arranged by size: 3950 1042 780 374 354 218 147 140 138 87 73 18 12 9 3 3 BseYI C'CCAG_C Cuts at: 3488 4106 4133 4448 4492 6551 7047 3488 Size: 618 27 315 44 2059 496 3789 Fragments arranged by size: 3789 2059 618 496 315 44 27 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2009 3056 3449 4092 4193 1163 Size: 573 273 1047 393 643 101 4318 Fragments arranged by size: 4318 1047 643 573 393 273 101 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3358 3613 4798 5559 6587 1540 Size: 275 186 1357 255 1185 761 1028 2301 Fragments arranged by size: 2301 1357 1185 1028 761 275 255 186 BsiHKAI G_wGCw'C Cuts at: 2342 2907 3135 3230 5409 5494 6565 2342 Size: 565 228 95 2179 85 1071 3125 Fragments arranged by size: 3125 2179 1071 565 228 95 85 BsmI GAATG_Cn' Cuts at: 4859 4952 4859 Size: 93 7255 BsmBI CGTCTCn'nnnn_ Cuts at: 2735 3263 2735 Size: 528 6820 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2274 2342 2838 2907 2923 3100 3135 Size: 399 484 68 496 69 16 177 35 Cuts at: 3135 3153 3230 3424 3450 3531 3908 3967 4239 Size: 18 77 194 26 81 377 59 272 Cuts at: 4239 4251 4377 4507 4544 4551 4579 4667 5409 Size: 12 126 130 37 7 28 88 742 Cuts at: 5409 5494 6565 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 496 484 399 377 272 194 177 130 126 88 85 81 77 69 68 59 37 35 28 26 18 16 12 7 BspEI T'CCGG_A Cuts at: 3990 7011 3990 Size: 3021 4327 BspHI T'CATG_A Cuts at: 5237 6967 5237 Size: 1730 5618 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2031 2172 2466 4703 844 Size: 1187 141 294 2237 3489 Fragments arranged by size: 3489 2237 1187 294 141 BsrBI CCG'CTC Cuts at: 1394 1823 2059 2218 2527 3328 5235 1394 Size: 429 236 159 309 801 1907 3507 Fragments arranged by size: 3507 1907 801 429 309 236 159 BsrDI GCAATG_nn' Cuts at: 65 1248 2178 5843 6017 65 Size: 1183 930 3665 174 1396 Fragments arranged by size: 3665 1396 1183 930 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2316 2342 3416 3612 5992 6179 1700 Size: 110 506 26 1074 196 2380 187 2869 Fragments arranged by size: 2869 2380 1074 506 196 187 110 26 BsrGI T'GTAC_A Cuts at: 96 3361 96 Size: 3265 4083 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7348 BssSI C'ACGA_G Cuts at: 2420 3128 5408 6420 2420 Size: 708 2280 1012 3348 Fragments arranged by size: 3348 2280 1012 708 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7348 BstBI TT'CG_AA Cuts at: 6152 6152 Size: 7348 BstEII G'GTnAC_C Cuts at: 1184 2185 3398 1184 Size: 1001 1213 5134 Fragments arranged by size: 5134 1213 1001 BstXI CCAn_nnnn'nTGG Cuts at: 3983 3983 Size: 7348 BstYI r'GATC_y Cuts at: 1972 2598 2808 3330 4434 4678 5045 5430 5447 Size: 626 210 522 1104 244 367 385 17 Cuts at: 5447 6899 6985 6997 7343 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1104 626 522 385 367 346 244 210 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3636 4341 3636 Size: 705 6643 BtgI C'CryG_G Cuts at: 513 1065 2969 4078 4429 513 Size: 552 1904 1109 351 3432 Fragments arranged by size: 3432 1904 1109 552 351 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6609 Fragments arranged by size: 6609 683 56 BtsI GCAGTG_nn' Cuts at: 2357 2890 3317 3512 4864 5658 5678 2357 Size: 533 427 195 1352 794 20 4027 Fragments arranged by size: 4027 1352 794 533 427 195 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7348 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7313 DraI TTT'AAA Cuts at: 4652 4652 Size: 7348 DraIII CAC_nnn'GTG Cuts at: 3986 3986 Size: 7348 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6355 809 Size: 507 5039 1802 Fragments arranged by size: 5039 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2364 2484 2721 Size: 54 1425 50 275 18 534 120 237 Cuts at: 2721 3090 3414 3954 4275 4795 5684 7313 8 Size: 369 324 540 321 520 889 1629 43 Fragments arranged by size: 1629 1425 889 540 534 520 369 324 321 275 237 120 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4795 1537 Size: 275 2983 4090 Fragments arranged by size: 4090 2983 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3176 3873 3888 4020 4197 4532 5278 1353 Size: 1823 697 15 132 177 335 746 3423 Fragments arranged by size: 3423 1823 746 697 335 177 132 15 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2651 3039 3551 5935 6309 6455 Size: 1064 138 1062 388 512 2384 374 146 Cuts at: 6455 387 Size: 1280 Fragments arranged by size: 2384 1280 1064 1062 512 388 374 146 138 EcoICRI GAG'CTC Cuts at: 2905 3133 2905 Size: 228 7120 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2075 2359 2750 2852 2863 2921 3020 Size: 393 443 284 391 102 11 58 99 Cuts at: 3020 3035 3497 3577 4110 4206 4400 5411 6008 Size: 15 462 80 533 96 194 1011 597 Cuts at: 6008 6795 7063 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1011 787 597 533 462 443 393 391 284 268 194 102 99 96 80 58 15 11 EcoNI CCTnn'n_nnAGG Cuts at: 3384 3384 Size: 7348 EcoO109I rG'GnC_Cy Cuts at: 2065 2919 2920 3377 3446 3527 3528 3963 4130 Size: 854 1 457 69 81 1 435 167 Cuts at: 4130 4235 4236 4255 4318 4411 4503 4582 4625 Size: 105 1 19 63 93 92 79 43 Cuts at: 4625 2065 Size: 4788 Fragments arranged by size: 4788 854 457 435 167 105 93 92 81 79 69 63 43 19 1 1 1 EcoRI G'AATT_C Cuts at: 4657 5035 4657 Size: 378 6970 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2464 2464 Size: 7348 FspI TGC'GCA Cuts at: 5854 5854 Size: 7348 HaeII r_GCGC'y Cuts at: 1678 2952 3108 3936 6495 1678 Size: 1274 156 828 2559 2531 Fragments arranged by size: 2559 2531 1274 828 156 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5989 6021 6063 6095 1373 Size: 32 135 32 4417 32 42 32 2626 Fragments arranged by size: 4417 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2125 4692 4938 669 Size: 988 281 187 2567 246 3079 Fragments arranged by size: 3079 2567 988 281 246 187 HindIII A'AGCT_T Cuts at: 748 748 Size: 7348 HpaI GTT'AAC Cuts at: 4938 4938 Size: 7348 KasI G'GCGC_C Cuts at: 2948 3932 2948 Size: 984 6364 KpnI G_GTAC'C Cuts at: 4673 4673 Size: 7348 MfeI C'AATT_G Cuts at: 2554 4947 2554 Size: 2393 4955 MluI A'CGCG_T Cuts at: 7224 7224 Size: 7348 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2655 6462 6646 1305 Size: 1350 3807 184 2007 Fragments arranged by size: 3807 2007 1350 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2366 2723 3092 3956 4277 Size: 54 1425 343 534 357 369 864 321 Cuts at: 4277 10 Size: 3081 Fragments arranged by size: 3081 1425 864 534 369 357 343 321 54 MslI CAynn'nnrTG Cuts at: 518 1166 2519 5306 5665 5824 518 Size: 648 1353 2787 359 159 2042 Fragments arranged by size: 2787 2042 1353 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2536 2584 2899 2971 2979 3463 3472 3952 Size: 930 48 315 72 8 484 9 480 Cuts at: 3952 4137 4764 5441 6589 6834 1606 Size: 185 627 677 1148 245 2120 Fragments arranged by size: 2120 1148 930 677 627 484 480 315 245 185 72 48 9 8 NarI GG'CG_CC Cuts at: 2949 3933 2949 Size: 984 6364 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6796 NdeI CA'TA_TG Cuts at: 387 387 Size: 7348 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7348 NotI GC'GGCC_GC Cuts at: 4795 4795 Size: 7348 NspI r_CATG'y Cuts at: 1132 1229 2770 3043 3206 4706 6251 1132 Size: 97 1541 273 163 1500 1545 2229 Fragments arranged by size: 2229 1545 1541 1500 273 163 97 PasI CC'CwG_GG Cuts at: 1348 2290 3901 4252 1348 Size: 942 1611 351 4444 Fragments arranged by size: 4444 1611 942 351 PciI A'CATG_T Cuts at: 2766 6247 2766 Size: 3481 3867 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 2719 4396 1108 Size: 902 709 1677 4060 Fragments arranged by size: 4060 1677 902 709 PfoI T'CCnGG_A Cuts at: 2691 2691 Size: 7348 PmeI GTTT'AAAC Cuts at: 4652 4652 Size: 7348 PmlI CAC'GTG Cuts at: 3076 3076 Size: 7348 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5417 5449 6930 6962 5417 Size: 32 1481 32 5803 Fragments arranged by size: 5803 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2065 4318 4411 2065 Size: 2253 93 5002 Fragments arranged by size: 5002 2253 93 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7348 PsiI TTA'TAA Cuts at: 4918 4918 Size: 7348 PspOMI G'GGCC_C Cuts at: 2919 3446 3527 3963 4235 4503 2919 Size: 527 81 436 272 268 5764 Fragments arranged by size: 5764 527 436 272 268 81 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2306 2338 2306 Size: 32 7316 PstI C_TGCA'G Cuts at: 830 2109 2154 2337 2469 3309 3654 4305 4700 Size: 1279 45 183 132 840 345 651 395 Cuts at: 4700 830 Size: 3478 Fragments arranged by size: 3478 1279 840 651 395 345 183 132 45 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7348 PvuII CAG'CTG Cuts at: 2536 2899 2979 3463 3472 3952 4137 2536 Size: 363 80 484 9 480 185 5747 Fragments arranged by size: 5747 484 480 363 185 80 9 SacI G_AGCT'C Cuts at: 2907 3135 2907 Size: 228 7120 SacII CC_GC'GG Cuts at: 2972 2972 Size: 7348 SalI G'TCGA_C Cuts at: 1936 4690 1936 Size: 2754 4594 SapI GCTCTTCn'nnn_ Cuts at: 4197 4532 4197 Size: 335 7013 SbfI CC_TGCA'GG Cuts at: 4700 4700 Size: 7348 ScaI AGT'ACT Cuts at: 5596 7308 5596 Size: 1712 5636 SexAI A'CCwGG_T Cuts at: 3564 3982 3564 Size: 418 6930 SfcI C'TryA_G Cuts at: 826 1045 2105 2150 2333 2465 3023 3305 3650 Size: 219 1060 45 183 132 558 282 345 Cuts at: 3650 4164 4301 4696 5831 6512 6703 826 Size: 514 137 395 1135 681 191 1471 Fragments arranged by size: 1471 1135 1060 681 558 514 395 345 282 219 191 183 137 132 45 SfiI GGCCn_nnn'nGGCC Cuts at: 2918 3962 2918 Size: 1044 6304 SfoI GGC'GCC Cuts at: 2950 3934 2950 Size: 984 6364 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7348 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7348 SmaI CCC'GGG Cuts at: 1872 4675 1872 Size: 2803 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2758 3617 3830 5452 6353 6615 Size: 197 926 815 859 213 1622 901 262 Cuts at: 6615 820 Size: 1553 Fragments arranged by size: 1622 1553 926 901 859 815 262 213 197 SnaBI TAC'GTA Cuts at: 493 5139 493 Size: 4646 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7348 SphI G_CATG'C Cuts at: 1132 3043 3206 4706 1132 Size: 1911 163 1500 3774 Fragments arranged by size: 3774 1911 1500 163 SspI AAT'ATT Cuts at: 5 52 5272 5 Size: 47 5220 2081 Fragments arranged by size: 5220 2081 47 StuI AGG'CCT Cuts at: 1517 3659 4394 1517 Size: 2142 735 4471 Fragments arranged by size: 4471 2142 735 StyI C'CwwG_G Cuts at: 513 1065 2914 4395 4551 4787 513 Size: 552 1849 1481 156 236 3074 Fragments arranged by size: 3074 1849 1481 552 236 156 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2204 2282 3590 5375 5392 5545 5730 Size: 1255 624 78 1308 1785 17 153 185 Cuts at: 5730 6175 325 Size: 445 1498 Fragments arranged by size: 1785 1498 1308 1255 624 445 185 153 78 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3361 5594 7306 Size: 274 80 33 51 1443 1384 2233 1712 Cuts at: 7306 96 Size: 138 Fragments arranged by size: 2233 1712 1443 1384 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3572 3604 3972 4004 4204 4236 4579 4611 7308 Size: 32 368 32 200 32 343 32 2697 Cuts at: 7308 7340 3572 Size: 32 3580 Fragments arranged by size: 3580 2697 368 343 200 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7348 XbaI T'CTAG_A Cuts at: 4684 4684 Size: 7348 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3160 3238 4134 4270 3160 Size: 78 896 136 6238 Fragments arranged by size: 6238 896 136 78 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7348 XmaI C'CCGG_G Cuts at: 1870 4673 1870 Size: 2803 4545 XmnI GAAnn'nnTTC Cuts at: 2873 5477 2873 Size: 2604 4744 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3339 5156 276 Size: 53 83 186 936 1805 1817 2468 Fragments arranged by size: 2468 1817 1805 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PspXI RsrII SanDI SrfI SwaI