Restriction map for
pFN21AA1114
##################################
# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII AccI AccIII AclI AcyI
AflII AflIII AhdI AocI AscI
AseI AsnI Asp700I AspEI BbeI
BbrPI BcgI BclI BfrI BsaAI
BsaHI BseAI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrGI BssHII Bst1107I BstBI
BstZ17I Bsu36I ClaI CpoI DraIII
DrdI EagI Eam1105I EclXI Eco52I
Eco81I EcoNI EcoRI FbaI FseI
Hin1I HpaI KasI KspI MluI
MroI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SnaBI Sse8387I
SspBI SwaI XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
Acc65I 3730 GGTACC G^GTACC
AcsI 930 AAATTC [GA]^AATT[CT]
AgeI 3583 ACCGGT A^CCGGT
Aor51HI 3736 AGCGCT AGC^GCT
ApoI 930 AAATTC [GA]^AATT[CT]
Asp718I 3730 GGTACC G^GTACC
AspI 118 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1676 TGCGCA TGC^GCA
AvrII 3666 CCTAGG C^CTAGG
BamHI 1283 GGATCC G^GATCC
BciVI 731 GTATCC GTATCC(6/5)
BglII 944 AGATCT A^GATCT
BlnI 3666 CCTAGG C^CTAGG
BsaWI 3583 ACCGGT [AT]^CCGG[AT]
BsmI 1707 GCATTC GAATGC(1/-1)
BsrDI 3599 GCAATG GCAATG(2/0)
BssSI 1837 CACGAG CACGAG(-5/-1)
BstAPI 247 GCACCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 369 GGTAACC G^GT[ACGT]ACC
BstPI 369 GGTAACC G^GT[ACGT]ACC
BstXI 474 CCATGAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 318 TTTAAA TTT^AAA
Eco47III 3736 AGCGCT AGC^GCT
EcoO65I 369 GGTAACC G^GT[ACGT]ACC
EcoRV 2050 GATATC GAT^ATC
EcoT22I 133 ATGCAT ATGCA^T
FauI 1005 GCGGG CCCGC(4/6)
FspI 1676 TGCGCA TGC^GCA
HaeII 3736 AGCGCT [GA]GCGC^[CT]
HindIII 611 AAGCTT A^AGCTT
KpnI 3730 GGTACC GGTAC^C
NsiI 133 ATGCAT ATGCA^T
PflFI 118 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 3583 ACCGGT A^CCGGT
SacI 3452 GAGCTC GAGCT^C
SapI 2822 GCTCTTC GCTCTTC(1/4)
ScaI 2956 AGTACT AGT^ACT
Tth111I 118 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccII 1313 CGCG CG^CG
1918 CGCG
BbsI 783 GAAGAC GAAGAC(2/6)
2632 GTCTTC
BglI 1039 GCCACTAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1648 GCCAGTGAGGC
BlpI 400 GCTCAGC GC^T[ACGT]AGC
2646 GCTTAGC
Bpu1102I 400 GCTCAGC GC^T[ACGT]AGC
2646 GCTTAGC
BpuAI 783 GAAGAC GAAGAC(2/6)
2632 GTCTTC
BsaBI 1171 GATGACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1386 GATCCCCATC
BseRI 1603 CTCCTC GAGGAG(10/8)
2174 GAGGAG
BsmFI 265 GTCCC GGGAC(10/14)
1783 GTCCC
BsrFI 191 ACCGGC [GA]^CCGG[CT]
3583 ACCGGT
BstUI 1313 CGCG CG^CG
1918 CGCG
CelII 400 GCTCAGC GC^T[ACGT]AGC
2646 GCTTAGC
Cfr10I 191 ACCGGC [GA]^CCGG[CT]
3583 ACCGGT
HgaI 844 GACGC GACGC(5/10)
3137 GCGTC
HincII 187 GTCAAC GT[CT]^[GA]AC
2161 GTCAAC
HindII 187 GTCAAC GT[CT]^[GA]AC
2161 GTCAAC
MaeII 1121 ACGT A^CGT
2159 ACGT
MamI 1171 GATGACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1386 GATCCCCATC
MfeI 554 CAATTG C^AATTG
788 CAATTG
MunI 554 CAATTG C^AATTG
788 CAATTG
MvnI 1313 CGCG CG^CG
1918 CGCG
NcoI 170 CCATGG C^CATGG
1288 CCATGG
SmaI 60 CCCGGG CCC^GGG
1214 CCCGGG
SmlI 1132 CTTGAG C^T[CT][GA]AG
1861 CTCAAG
SpeI 2407 ACTAGT A^CTAGT
3853 ACTAGT
SphI 1870 GCATGC GCATG^C
2444 GCATGC
SspI 541 AATATT AAT^ATT
1439 AATATT
TfiI 1552 GAATC G^A[AT]TC
1614 GATTC
XmaCI 60 CCCGGG C^CCGGG
1214 CCCGGG
XmaI 60 CCCGGG C^CCGGG
1214 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AciI AfaI AluI Alw44I AlwI
AlwNI ApaI ApaLI AspHI AvaI
AvaII BalI BanI BanII BbvCI
BbvI BcnI BfaI BmyI BpmI
BsaI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI BsoBI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StuI StyI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII