(Circular) (Six-base) MAPSORT of: pFN21AA1114.seq Check: 5 from: 1 to: 8998 pFN21AA1114 8998 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2263 2263 Size: 8998 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6808 278 Size: 53 83 186 936 5272 2468 Fragments arranged by size: 5272 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 6341 1937 Size: 4404 4594 Acc65I G'GTAC_C Cuts at: 5735 6319 5735 Size: 584 8414 AclI AA'CG_TT Cuts at: 1563 7125 7498 1563 Size: 5562 373 3063 Fragments arranged by size: 5562 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2379 2522 3996 4156 4186 4240 4294 4348 Size: 747 143 1474 160 30 54 54 54 Cuts at: 4348 4624 5224 5254 5674 5794 6058 6088 6145 Size: 276 600 30 420 120 264 30 57 Cuts at: 6145 7061 8445 8713 1632 Size: 916 1384 268 1917 Fragments arranged by size: 1917 1474 1384 916 747 600 420 276 268 264 160 143 120 57 54 54 54 30 30 30 AfeI AGC'GCT Cuts at: 5743 5743 Size: 8998 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8801 AflIII A'CryG_T Cuts at: 1203 7897 8874 1203 Size: 6694 977 1327 Fragments arranged by size: 6694 1327 977 AgeI A'CCGG_T Cuts at: 5588 7829 5588 Size: 2241 6757 AhdI GACnn_n'nnGTC Cuts at: 7727 7727 Size: 8998 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8998 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8998 AlwNI CAG_nnn'CTG Cuts at: 2402 3169 4695 4755 4875 5025 5145 5445 5475 Size: 767 1526 60 120 150 120 300 30 Cuts at: 5475 5535 6185 6741 8313 2402 Size: 60 650 556 1572 3087 Fragments arranged by size: 3087 1572 1526 767 650 556 300 150 120 120 60 60 30 ApaI G_GGCC'C Cuts at: 2054 2933 3097 4035 2054 Size: 879 164 938 7017 Fragments arranged by size: 7017 938 879 164 ApaLI G'TGCA_C Cuts at: 4233 4395 4617 5067 5187 5277 7055 8211 4233 Size: 162 222 450 120 90 1778 1156 5020 Fragments arranged by size: 5020 1778 1156 450 222 162 120 90 ApoI r'AATT_y Cuts at: 1493 2935 6307 6533 6685 8794 1493 Size: 1442 3372 226 152 2109 1697 Fragments arranged by size: 3372 2109 1697 1442 226 152 AseI AT'TA_AT Cuts at: 160 1032 7552 160 Size: 872 6520 1606 Fragments arranged by size: 6520 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8998 AvaI C'yCGr_G Cuts at: 1870 1943 2065 3054 3219 6323 1870 Size: 73 122 989 165 3104 4545 Fragments arranged by size: 4545 3104 989 165 122 73 AvrII C'CTAG_G Cuts at: 5671 5671 Size: 8998 BamHI G'GATC_C Cuts at: 3288 6328 6695 3288 Size: 3040 367 5591 Fragments arranged by size: 5591 3040 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2035 5045 5435 5675 5735 Size: 325 217 627 248 3010 390 240 60 Cuts at: 5735 5765 5795 5825 5885 5915 6275 6319 7774 Size: 30 30 30 60 30 360 44 1455 Cuts at: 7774 618 Size: 1842 Fragments arranged by size: 3010 1842 1455 627 390 360 325 248 240 217 60 60 44 30 30 30 30 BanII G_rGCy'C Cuts at: 1391 2054 2933 3034 3097 3207 4000 4035 4138 Size: 663 879 101 63 110 793 35 103 Cuts at: 4138 5461 6317 1391 Size: 1323 856 4072 Fragments arranged by size: 4072 1323 879 856 793 663 110 103 101 63 35 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2795 4630 928 Size: 971 896 1835 5296 Fragments arranged by size: 5296 1835 971 896 BbvCI CC'TCA_GC Cuts at: 3416 3969 3987 5942 3416 Size: 553 18 1955 6472 Fragments arranged by size: 6472 1955 553 18 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 7189 7223 1903 Size: 34 5252 34 3678 Fragments arranged by size: 5252 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2747 6890 8100 2747 Size: 4143 1210 3645 Fragments arranged by size: 4143 3645 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 8998 BfrBI ATG'CAT Cuts at: 2140 2140 Size: 8998 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3050 3659 7609 Size: 107 122 71 631 605 1378 609 3950 Cuts at: 7609 136 Size: 1525 Fragments arranged by size: 3950 1525 1378 631 609 605 122 107 71 BglII A'GATC_T Cuts at: 2949 8993 2949 Size: 6044 2954 BlpI GC'TnA_GC Cuts at: 2406 4652 6415 2406 Size: 2246 1763 4989 Fragments arranged by size: 4989 2246 1763 Bme1580I G_kGCm'C Cuts at: 1790 2054 2933 3097 3136 4035 4237 4399 4621 Size: 264 879 164 39 899 202 162 222 Cuts at: 4621 5071 5191 5281 5341 5798 5828 5888 7059 Size: 450 120 90 60 457 30 60 1171 Cuts at: 7059 8215 1790 Size: 1156 2573 Fragments arranged by size: 2573 1171 1156 899 879 457 450 264 222 202 164 162 120 90 60 60 39 30 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8998 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3584 3916 5801 Size: 416 53 83 399 24 2167 332 1885 Cuts at: 5801 5831 5891 6081 6729 7687 442 Size: 30 60 190 648 958 1753 Fragments arranged by size: 2167 1885 1753 958 648 416 399 332 190 83 60 53 30 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8998 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4577 4697 4577 Size: 120 8878 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2076 2250 3221 3503 3794 4123 4228 4342 Size: 837 174 971 282 291 329 105 114 Cuts at: 4342 4702 7658 1239 Size: 360 2956 2579 Fragments arranged by size: 2956 2579 971 837 360 329 291 282 174 114 105 Bpu10I CC'TnA_GC Cuts at: 2799 3416 3969 3987 4095 5702 5942 2799 Size: 617 553 18 108 1607 240 5855 Fragments arranged by size: 5855 1607 617 553 240 108 18 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3158 3851 7123 7988 8286 3158 Size: 693 3272 865 298 3870 Fragments arranged by size: 3870 3272 865 693 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2102 2674 3611 7661 882 Size: 617 603 572 937 4050 2219 Fragments arranged by size: 4050 2219 937 617 603 572 BsaAI yAC'GTr Cuts at: 493 1204 6789 493 Size: 711 5585 2702 Fragments arranged by size: 5585 2702 711 BsaBI GATnn'nnATC Cuts at: 3180 3395 8992 3180 Size: 215 5597 3186 Fragments arranged by size: 5597 3186 215 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6805 7187 7822 275 Size: 53 83 186 936 5272 382 635 1451 Fragments arranged by size: 5272 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5588 7431 7829 8103 8250 8661 5588 Size: 1843 398 274 147 411 5925 Fragments arranged by size: 5925 1843 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3476 3506 1178 Size: 30 165 30 2073 30 6670 Fragments arranged by size: 6670 2073 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3599 4194 1186 Size: 2413 595 5990 Fragments arranged by size: 5990 2413 595 BseYI C'CCAG_C Cuts at: 2272 3601 4028 4238 4292 4712 4892 5162 5372 Size: 1329 427 210 54 420 180 270 210 Cuts at: 5372 5402 5462 5576 6056 8201 8697 2272 Size: 30 60 114 480 2145 496 2573 Fragments arranged by size: 2573 2145 1329 496 480 427 420 270 210 210 180 114 60 54 30 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2235 3294 3905 3935 4759 1163 Size: 573 499 1059 611 30 824 5402 Fragments arranged by size: 5402 1059 824 611 573 499 30 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6448 7209 8237 1540 Size: 275 186 4447 761 1028 2301 Fragments arranged by size: 4447 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4237 4352 4399 4621 4634 4921 4953 4988 5071 Size: 115 47 222 13 287 32 35 83 Cuts at: 5071 5191 5281 5431 5461 6179 6239 7059 7144 Size: 120 90 150 30 718 60 820 85 Cuts at: 7144 8215 4237 Size: 1071 5020 Fragments arranged by size: 5020 1071 820 718 287 222 150 120 115 90 85 83 60 47 35 32 30 13 BsmI GAATG_Cn' Cuts at: 3712 6509 6602 3712 Size: 2797 93 6108 Fragments arranged by size: 6108 2797 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2054 2933 3034 3097 3136 3207 4000 Size: 399 264 879 101 63 39 71 793 Cuts at: 4000 4035 4138 4237 4352 4399 4621 4634 4921 Size: 35 103 99 115 47 222 13 287 Cuts at: 4921 4953 4988 5071 5191 5281 5341 5431 5461 Size: 32 35 83 120 90 60 90 30 Cuts at: 5461 5798 5828 5888 6179 6239 6317 7059 7144 Size: 337 30 60 291 60 78 742 85 Cuts at: 7144 8215 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 879 793 742 399 337 291 287 264 222 120 115 103 101 99 90 90 85 83 78 71 63 60 60 60 47 39 35 35 32 30 30 13 BspEI T'CCGG_A Cuts at: 8661 8661 Size: 8998 BspHI T'CATG_A Cuts at: 6887 8617 6887 Size: 1730 7268 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2263 2337 2506 6353 844 Size: 1419 74 169 3847 3489 Fragments arranged by size: 3847 3489 1419 169 74 BsrBI CCG'CTC Cuts at: 1394 1823 6885 1394 Size: 429 5062 3507 Fragments arranged by size: 5062 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5611 7493 7667 65 Size: 1183 4363 1882 174 1396 Fragments arranged by size: 4363 1882 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2196 5588 7642 7829 1700 Size: 110 386 3392 2054 187 2869 Fragments arranged by size: 3392 2869 2054 386 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8998 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8998 BssSI C'ACGA_G Cuts at: 3842 7058 8070 3842 Size: 3216 1012 4770 Fragments arranged by size: 4770 3216 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2258 794 Size: 1464 7534 BstBI TT'CG_AA Cuts at: 7802 7802 Size: 8998 BstEII G'GTnAC_C Cuts at: 1184 2374 1184 Size: 1190 7808 BstXI CCAn_nnnn'nTGG Cuts at: 2486 2486 Size: 8998 BstYI r'GATC_y Cuts at: 1972 2949 3288 3390 6328 6695 7080 7097 8549 Size: 977 339 102 2938 367 385 17 1452 Cuts at: 8549 8635 8647 8993 1972 Size: 86 12 346 1977 Fragments arranged by size: 2938 1977 1452 977 385 367 346 339 102 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2175 3293 4569 513 Size: 552 939 171 1118 1276 4942 Fragments arranged by size: 4942 1276 1118 939 552 171 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4101 4221 5967 487 Size: 683 56 2875 120 1746 3518 Fragments arranged by size: 3518 2875 1746 683 120 56 BtsI GCAGTG_nn' Cuts at: 2400 3936 4429 4621 4951 5071 5491 6025 6145 Size: 1536 493 192 330 120 420 534 120 Cuts at: 6145 6262 6514 7308 7328 2400 Size: 117 252 794 20 4070 Fragments arranged by size: 4070 1536 794 534 493 420 330 252 192 120 120 117 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8998 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4834 4869 529 Size: 35 4270 35 4658 Fragments arranged by size: 4658 4270 35 35 DraI TTT'AAA Cuts at: 2325 6302 2325 Size: 3977 5021 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 8005 809 Size: 507 6689 1802 Fragments arranged by size: 6689 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2172 2476 3327 Size: 54 1425 50 275 18 342 304 851 Cuts at: 3327 6445 7334 8963 8 Size: 3118 889 1629 43 Fragments arranged by size: 3118 1629 1425 889 851 342 304 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6445 1537 Size: 275 4633 4090 Fragments arranged by size: 4633 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2112 2426 4071 4834 6928 1353 Size: 759 314 1645 763 2094 3423 Fragments arranged by size: 3423 2094 1645 763 759 314 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3301 5832 7585 7959 8105 387 Size: 1064 138 1712 2531 1753 374 146 1280 Fragments arranged by size: 2531 1753 1712 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 5459 5459 Size: 8998 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2076 2250 2379 2522 3221 3503 3794 Size: 393 444 174 129 143 699 282 291 Cuts at: 3794 3996 4123 4156 4186 4228 4240 4294 4342 Size: 202 127 33 30 42 12 54 48 Cuts at: 4342 4348 4624 4702 5224 5254 5674 5794 6058 Size: 6 276 78 522 30 420 120 264 Cuts at: 6058 6088 6145 7061 7658 8445 8713 1239 Size: 30 57 916 597 787 268 1524 Fragments arranged by size: 1524 916 787 699 597 522 444 420 393 291 282 276 268 264 202 174 143 129 127 120 78 57 54 48 42 33 30 30 30 12 6 EcoO109I rG'GnC_Cy Cuts at: 2050 2051 2224 2518 2929 2959 3093 3166 3216 Size: 1 173 294 411 30 134 73 50 Cuts at: 3216 3787 4032 2050 Size: 571 245 7016 Fragments arranged by size: 7016 571 411 294 245 173 134 73 50 30 1 EcoRI G'AATT_C Cuts at: 6307 6685 6307 Size: 378 8620 EcoRV GAT'ATC Cuts at: 4057 4057 Size: 8998 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2527 2527 Size: 8998 FspI TGC'GCA Cuts at: 3683 7504 3683 Size: 3821 5177 HaeII r_GCGC'y Cuts at: 1678 5745 8145 1678 Size: 4067 2400 2531 Fragments arranged by size: 4067 2531 2400 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 7639 7671 7713 7745 1373 Size: 32 135 32 6067 32 42 32 2626 Fragments arranged by size: 6067 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2194 4168 6342 6588 669 Size: 988 281 256 1974 2174 246 3079 Fragments arranged by size: 3079 2174 1974 988 281 256 246 HindIII A'AGCT_T Cuts at: 748 2616 748 Size: 1868 7130 HpaI GTT'AAC Cuts at: 6588 6588 Size: 8998 KpnI G_GTAC'C Cuts at: 5739 6323 5739 Size: 584 8414 MfeI C'AATT_G Cuts at: 2559 2793 6597 2559 Size: 234 3804 4960 Fragments arranged by size: 4960 3804 234 MluI A'CGCG_T Cuts at: 8874 8874 Size: 8998 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6051 8112 8296 1305 Size: 4746 2061 184 2007 Fragments arranged by size: 4746 2061 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2174 2478 3329 10 Size: 54 1425 343 342 304 851 5679 Fragments arranged by size: 5679 1425 851 343 342 304 54 MslI CAynn'nnrTG Cuts at: 518 1166 2484 3252 3635 6956 7315 7474 518 Size: 648 1318 768 383 3321 359 159 2042 Fragments arranged by size: 3321 2042 1318 768 648 383 359 159 MspA1I CmG'CkG Cuts at: 1606 2276 2500 3956 5351 6108 6165 6414 7091 Size: 670 224 1456 1395 757 57 249 677 Cuts at: 7091 8239 8484 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1456 1395 1148 757 677 670 249 245 224 57 NcoI C'CATG_G Cuts at: 513 1065 2004 2175 3293 513 Size: 552 939 171 1118 6218 Fragments arranged by size: 6218 1118 939 552 171 NdeI CA'TA_TG Cuts at: 387 387 Size: 8998 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8998 NotI GC'GGCC_GC Cuts at: 6445 6445 Size: 8998 NsiI A_TGCA'T Cuts at: 2142 2142 Size: 8998 NspI r_CATG'y Cuts at: 1132 1229 3415 3879 4453 6356 7901 1132 Size: 97 2186 464 574 1903 1545 2229 Fragments arranged by size: 2229 2186 1903 1545 574 464 97 PasI CC'CwG_GG Cuts at: 1348 3701 1348 Size: 2353 6645 PciI A'CATG_T Cuts at: 7897 7897 Size: 8998 PflMI CCAn_nnn'nTGG Cuts at: 1108 2995 3169 3906 6122 6209 1108 Size: 1887 174 737 2216 87 3897 Fragments arranged by size: 3897 2216 1887 737 174 87 PmeI GTTT'AAAC Cuts at: 6302 6302 Size: 8998 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7067 7099 8580 8612 7067 Size: 32 1481 32 7453 Fragments arranged by size: 7453 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2518 3216 3787 2518 Size: 698 571 7729 Fragments arranged by size: 7729 698 571 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8998 PsiI TTA'TAA Cuts at: 6568 6568 Size: 8998 PspOMI G'GGCC_C Cuts at: 2050 2929 3093 4031 2050 Size: 879 164 938 7017 Fragments arranged by size: 7017 938 879 164 PstI C_TGCA'G Cuts at: 830 3568 3955 6140 6350 830 Size: 2738 387 2185 210 3478 Fragments arranged by size: 3478 2738 2185 387 210 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8998 PvuII CAG'CTG Cuts at: 2276 2500 3956 2276 Size: 224 1456 7318 Fragments arranged by size: 7318 1456 224 SacI G_AGCT'C Cuts at: 5461 5461 Size: 8998 SalI G'TCGA_C Cuts at: 1936 6340 1936 Size: 4404 4594 SanDI GG'GwC_CC Cuts at: 3787 3787 Size: 8998 SapI GCTCTTCn'nnn_ Cuts at: 4834 4834 Size: 8998 SbfI CC_TGCA'GG Cuts at: 6350 6350 Size: 8998 ScaI AGT'ACT Cuts at: 4963 7246 8958 4963 Size: 2283 1712 5003 Fragments arranged by size: 5003 2283 1712 SfcI C'TryA_G Cuts at: 826 1045 3564 3951 6136 6346 7481 8162 8353 Size: 219 2519 387 2185 210 1135 681 191 Cuts at: 8353 826 Size: 1471 Fragments arranged by size: 2519 2185 1471 1135 681 387 219 210 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8998 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8998 SmaI CCC'GGG Cuts at: 1872 2067 3221 6325 1872 Size: 195 1154 3104 4545 Fragments arranged by size: 4545 3104 1154 195 SmlI C'TyrA_G Cuts at: 820 1017 1943 3137 3866 7102 8003 8265 820 Size: 197 926 1194 729 3236 901 262 1553 Fragments arranged by size: 3236 1553 1194 926 901 729 262 197 SnaBI TAC'GTA Cuts at: 493 6789 493 Size: 6296 2702 SpeI A'CTAG_T Cuts at: 152 4412 5858 152 Size: 4260 1446 3292 Fragments arranged by size: 4260 3292 1446 SphI G_CATG'C Cuts at: 1132 3879 4453 6356 1132 Size: 2747 574 1903 3774 Fragments arranged by size: 3774 2747 1903 574 SspI AAT'ATT Cuts at: 5 52 2548 3446 6922 5 Size: 47 2496 898 3476 2081 Fragments arranged by size: 3476 2496 2081 898 47 StuI AGG'CCT Cuts at: 1517 2873 3307 4100 5971 1517 Size: 1356 434 793 1871 4544 Fragments arranged by size: 4544 1871 1356 793 434 StyI C'CwwG_G Cuts at: 513 1065 2004 2160 2175 2445 2455 2622 2707 Size: 552 939 156 15 270 10 167 85 Cuts at: 2707 2904 3088 3293 3585 3900 5671 6437 513 Size: 197 184 205 292 315 1771 766 3074 Fragments arranged by size: 3074 1771 939 766 552 315 292 270 205 197 184 167 156 85 15 10 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2705 2720 3610 4252 4306 4636 6187 Size: 1255 1125 15 890 642 54 330 1551 Cuts at: 6187 6247 7025 7042 7195 7380 7825 325 Size: 60 778 17 153 185 445 1498 Fragments arranged by size: 1551 1498 1255 1125 890 778 642 445 330 185 153 60 54 17 15 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3777 4588 4961 Size: 274 80 33 51 1443 1800 811 373 Cuts at: 4961 7244 8956 96 Size: 2283 1712 138 Fragments arranged by size: 2283 1800 1712 1443 811 373 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2019 2051 8958 8990 2019 Size: 32 6907 32 2027 Fragments arranged by size: 6907 2027 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2126 1316 Size: 810 8188 XbaI T'CTAG_A Cuts at: 6334 6334 Size: 8998 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2167 2471 4007 2167 Size: 304 1536 7158 Fragments arranged by size: 7158 1536 304 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8998 XmaI C'CCGG_G Cuts at: 1870 2065 3219 6323 1870 Size: 195 1154 3104 4545 Fragments arranged by size: 4545 3104 1154 195 XmnI GAAnn'nnTTC Cuts at: 7127 7127 Size: 8998 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6806 276 Size: 53 83 186 936 5272 2468 Fragments arranged by size: 5272 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI BaeI BbeI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspXI PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI