Restriction map for
pFN21AA1114
(Linear) (Six-base) MAPSORT of: KIAA1114.seq Check: 8668 from: 1 to: 4293
KIAA1114.orf
With 182 enzymes: SgfI *
February 28, 2007 11:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 258 4293
Size: 258 4035
Acc65I G'GTAC_C
Cuts at: 0 3730 4293
Size: 3730 563
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 374 517 1991 2151 2181 2235 2289 2343
Size: 374 143 1474 160 30 54 54 54
Cuts at: 2343 2619 3219 3249 3669 3789 4053 4083 4140
Size: 276 600 30 420 120 264 30 57
Cuts at: 4140 4293
Size: 153
Fragments arranged by size:
1474 600 420 374 276 264 160 153
143 120 57 54 54 54 30 30
30
AfeI AGC'GCT
Cuts at: 0 3738 4293
Size: 3738 555
AgeI A'CCGG_T
Cuts at: 0 3583 4293
Size: 3583 710
AlwNI CAG_nnn'CTG
Cuts at: 0 397 1164 2690 2750 2870 3020 3140 3440
Size: 397 767 1526 60 120 150 120 300
Cuts at: 3440 3470 3530 4180 4293
Size: 30 60 650 113
Fragments arranged by size:
1526 767 650 397 300 150 120 120
113 60 60 30
ApaI G_GGCC'C
Cuts at: 0 49 928 1092 2030 4293
Size: 49 879 164 938 2263
Fragments arranged by size:
2263 938 879 164 49
ApaLI G'TGCA_C
Cuts at: 0 2228 2390 2612 3062 3182 3272 4293
Size: 2228 162 222 450 120 90 1021
Fragments arranged by size:
2228 1021 450 222 162 120 90
ApoI r'AATT_y
Cuts at: 0 930 4293
Size: 930 3363
AvaI C'yCGr_G
Cuts at: 0 60 1049 1214 4293
Size: 60 989 165 3079
Fragments arranged by size:
3079 989 165 60
AvrII C'CTAG_G
Cuts at: 0 3666 4293
Size: 3666 627
BamHI G'GATC_C
Cuts at: 0 1283 4293
Size: 1283 3010
BanI G'GyrC_C
Cuts at: 0 30 3040 3430 3670 3730 3760 3790 3820
Size: 30 3010 390 240 60 30 30 30
Cuts at: 3820 3880 3910 4270 4293
Size: 60 30 360 23
Fragments arranged by size:
3010 390 360 240 60 60 30 30
30 30 30 23
BanII G_rGCy'C
Cuts at: 0 49 928 1029 1092 1202 1995 2030 2133
Size: 49 879 101 63 110 793 35 103
Cuts at: 2133 3456 4293
Size: 1323 837
Fragments arranged by size:
1323 879 837 793 110 103 101 63
49 35
BbsI GAAGACnn'nnnn_
Cuts at: 0 790 2625 4293
Size: 790 1835 1668
Fragments arranged by size:
1835 1668 790
BbvCI CC'TCA_GC
Cuts at: 0 1411 1964 1982 3937 4293
Size: 1411 553 18 1955 356
Fragments arranged by size:
1955 1411 553 356 18
BciVI GTATCCnnnnn_n'
Cuts at: 0 742 4293
Size: 742 3551
BfrBI ATG'CAT
Cuts at: 0 135 4293
Size: 135 4158
BglI GCCn_nnn'nGGC
Cuts at: 0 1045 1654 4293
Size: 1045 609 2639
Fragments arranged by size:
2639 1045 609
BglII A'GATC_T
Cuts at: 0 944 4293
Size: 944 3349
BlpI GC'TnA_GC
Cuts at: 0 401 2647 4293
Size: 401 2246 1646
Fragments arranged by size:
2246 1646 401
Bme1580I G_kGCm'C
Cuts at: 0 49 928 1092 1131 2030 2232 2394 2616
Size: 49 879 164 39 899 202 162 222
Cuts at: 2616 3066 3186 3276 3336 3793 3823 3883 4293
Size: 450 120 90 60 457 30 60 410
Fragments arranged by size:
899 879 457 450 410 222 202 164
162 120 90 60 60 49 39 30
BmrI ACTGGGnnnn_n'
Cuts at: 0 1579 1911 3796 3826 3886 4076 4293
Size: 1579 332 1885 30 60 190 217
Fragments arranged by size:
1885 1579 332 217 190 60 30
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2572 2692 4293
Size: 2572 120 1601
Fragments arranged by size:
2572 1601 120
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 71 245 1216 1498 1789 2118 2223 2337
Size: 71 174 971 282 291 329 105 114
Cuts at: 2337 2697 4293
Size: 360 1596
Fragments arranged by size:
1596 971 360 329 291 282 174 114
105 71
Bpu10I CC'TnA_GC
Cuts at: 0 794 1411 1964 1982 2090 3697 3937 4293
Size: 794 617 553 18 108 1607 240 356
Fragments arranged by size:
1607 794 617 553 356 240 108 18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1153 1846 4293
Size: 1153 693 2447
Fragments arranged by size:
2447 1153 693
BsaI GGTCTCn'nnnn_
Cuts at: 0 97 669 1606 4293
Size: 97 572 937 2687
Fragments arranged by size:
2687 937 572 97
BsaBI GATnn'nnATC
Cuts at: 0 1175 1390 4293
Size: 1175 215 2903
Fragments arranged by size:
2903 1175 215
BsaWI w'CCGG_w
Cuts at: 0 3583 4293
Size: 3583 710
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1471 1501 4293
Size: 1471 30 2792
Fragments arranged by size:
2792 1471 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1594 2189 4293
Size: 1594 595 2104
Fragments arranged by size:
2104 1594 595
BseYI C'CCAG_C
Cuts at: 0 267 1596 2023 2233 2287 2707 2887 3157
Size: 267 1329 427 210 54 420 180 270
Cuts at: 3157 3367 3397 3457 3571 4051 4293
Size: 210 30 60 114 480 242
Fragments arranged by size:
1329 480 427 420 270 267 242 210
210 180 114 60 54 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 230 1289 1900 1930 2754 4293
Size: 230 1059 611 30 824 1539
Fragments arranged by size:
1539 1059 824 611 230 30
BsiHKAI G_wGCw'C
Cuts at: 0 2232 2347 2394 2616 2629 2916 2948 2983
Size: 2232 115 47 222 13 287 32 35
Cuts at: 2983 3066 3186 3276 3426 3456 4174 4234 4293
Size: 83 120 90 150 30 718 60 59
Fragments arranged by size:
2232 718 287 222 150 120 115 90
83 60 59 47 35 32 30 13
BsmI GAATG_Cn'
Cuts at: 0 1707 4293
Size: 1707 2586
Bsp1286I G_dGCh'C
Cuts at: 0 49 928 1029 1092 1131 1202 1995 2030
Size: 49 879 101 63 39 71 793 35
Cuts at: 2030 2133 2232 2347 2394 2616 2629 2916 2948
Size: 103 99 115 47 222 13 287 32
Cuts at: 2948 2983 3066 3186 3276 3336 3426 3456 3793
Size: 35 83 120 90 60 90 30 337
Cuts at: 3793 3823 3883 4174 4234 4293
Size: 30 60 291 60 59
Fragments arranged by size:
879 793 337 291 287 222 120 115
103 101 99 90 90 83 71 63
60 60 60 59 49 47 39 35
35 32 30 30 13
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 258 332 501 4293
Size: 258 74 169 3792
Fragments arranged by size:
3792 258 169 74
BsrDI GCAATG_nn'
Cuts at: 0 3606 4293
Size: 3606 687
BsrFI r'CCGG_y
Cuts at: 0 191 3583 4293
Size: 191 3392 710
Fragments arranged by size:
3392 710 191
BssSI C'ACGA_G
Cuts at: 0 1837 4293
Size: 1837 2456
BstAPI GCAn_nnn'nTGC
Cuts at: 0 253 4293
Size: 253 4040
BstEII G'GTnAC_C
Cuts at: 0 369 4293
Size: 369 3924
BstXI CCAn_nnnn'nTGG
Cuts at: 0 481 4293
Size: 481 3812
BstYI r'GATC_y
Cuts at: 0 944 1283 1385 4293
Size: 944 339 102 2908
Fragments arranged by size:
2908 944 339 102
BtgI C'CryG_G
Cuts at: 0 170 1288 2564 4293
Size: 170 1118 1276 1729
Fragments arranged by size:
1729 1276 1118 170
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2096 2216 3962 4293
Size: 2096 120 1746 331
Fragments arranged by size:
2096 1746 331 120
BtsI GCAGTG_nn'
Cuts at: 0 395 1931 2424 2616 2946 3066 3486 4020
Size: 395 1536 493 192 330 120 420 534
Cuts at: 4020 4140 4257 4293
Size: 120 117 36
Fragments arranged by size:
1536 534 493 420 395 330 192 120
120 117 36
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2829 2864 4293
Size: 2829 35 1429
Fragments arranged by size:
2829 1429 35
DraI TTT'AAA
Cuts at: 0 320 4293
Size: 320 3973
EaeI y'GGCC_r
Cuts at: 0 167 471 1322 4293
Size: 167 304 851 2971
Fragments arranged by size:
2971 851 304 167
EarI CTCTTCn'nnn_
Cuts at: 0 107 421 2066 2829 4293
Size: 107 314 1645 763 1464
Fragments arranged by size:
1645 1464 763 314 107
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1296 3827 4293
Size: 1296 2531 466
Fragments arranged by size:
2531 1296 466
EcoICRI GAG'CTC
Cuts at: 0 3454 4293
Size: 3454 839
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 71 245 374 517 1216 1498 1789 1991
Size: 71 174 129 143 699 282 291 202
Cuts at: 1991 2118 2151 2181 2223 2235 2289 2337 2343
Size: 127 33 30 42 12 54 48 6
Cuts at: 2343 2619 2697 3219 3249 3669 3789 4053 4083
Size: 276 78 522 30 420 120 264 30
Cuts at: 4083 4140 4293
Size: 57 153
Fragments arranged by size:
699 522 420 291 282 276 264 202
174 153 143 129 127 120 78 71
57 54 48 42 33 30 30 30
12 6
EcoO109I rG'GnC_Cy
Cuts at: 0 45 46 219 513 924 954 1088 1161
Size: 45 1 173 294 411 30 134 73
Cuts at: 1161 1211 1782 2027 4293
Size: 50 571 245 2266
Fragments arranged by size:
2266 571 411 294 245 173 134 73
50 45 30 1
EcoRV GAT'ATC
Cuts at: 0 2052 4293
Size: 2052 2241
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 522 4293
Size: 522 3771
FspI TGC'GCA
Cuts at: 0 1678 4293
Size: 1678 2615
HaeII r_GCGC'y
Cuts at: 0 3740 4293
Size: 3740 553
HincII GTy'rAC
Cuts at: 0 189 2163 4293
Size: 189 1974 2130
Fragments arranged by size:
2130 1974 189
HindIII A'AGCT_T
Cuts at: 0 611 4293
Size: 611 3682
KpnI G_GTAC'C
Cuts at: 0 3734 4293
Size: 3734 559
MfeI C'AATT_G
Cuts at: 0 554 788 4293
Size: 554 234 3505
Fragments arranged by size:
3505 554 234
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 4046 4293
Size: 4046 247
MscI TGG'CCA
Cuts at: 0 169 473 1324 4293
Size: 169 304 851 2969
Fragments arranged by size:
2969 851 304 169
MslI CAynn'nnrTG
Cuts at: 0 479 1247 1630 4293
Size: 479 768 383 2663
Fragments arranged by size:
2663 768 479 383
MspA1I CmG'CkG
Cuts at: 0 271 495 1951 3346 4103 4160 4293
Size: 271 224 1456 1395 757 57 133
Fragments arranged by size:
1456 1395 757 271 224 133 57
NcoI C'CATG_G
Cuts at: 0 170 1288 4293
Size: 170 1118 3005
Fragments arranged by size:
3005 1118 170
NsiI A_TGCA'T
Cuts at: 0 137 4293
Size: 137 4156
NspI r_CATG'y
Cuts at: 0 1410 1874 2448 4293
Size: 1410 464 574 1845
Fragments arranged by size:
1845 1410 574 464
PasI CC'CwG_GG
Cuts at: 0 1696 4293
Size: 1696 2597
PflMI CCAn_nnn'nTGG
Cuts at: 0 990 1164 1901 4117 4204 4293
Size: 990 174 737 2216 87 89
Fragments arranged by size:
2216 990 737 174 89 87
PpuMI rG'GwC_Cy
Cuts at: 0 513 1211 1782 4293
Size: 513 698 571 2511
Fragments arranged by size:
2511 698 571 513
PspOMI G'GGCC_C
Cuts at: 0 45 924 1088 2026 4293
Size: 45 879 164 938 2267
Fragments arranged by size:
2267 938 879 164 45
PstI C_TGCA'G
Cuts at: 0 1563 1950 4135 4293
Size: 1563 387 2185 158
Fragments arranged by size:
2185 1563 387 158
PvuII CAG'CTG
Cuts at: 0 271 495 1951 4293
Size: 271 224 1456 2342
Fragments arranged by size:
2342 1456 271 224
SacI G_AGCT'C
Cuts at: 0 3456 4293
Size: 3456 837
SanDI GG'GwC_CC
Cuts at: 0 1782 4293
Size: 1782 2511
SapI GCTCTTCn'nnn_
Cuts at: 0 2829 4293
Size: 2829 1464
ScaI AGT'ACT
Cuts at: 0 2958 4293
Size: 2958 1335
SfcI C'TryA_G
Cuts at: 0 1559 1946 4131 4293
Size: 1559 387 2185 162
Fragments arranged by size:
2185 1559 387 162
SmaI CCC'GGG
Cuts at: 0 62 1216 4293
Size: 62 1154 3077
Fragments arranged by size:
3077 1154 62
SmlI C'TyrA_G
Cuts at: 0 1132 1861 4293
Size: 1132 729 2432
Fragments arranged by size:
2432 1132 729
SpeI A'CTAG_T
Cuts at: 0 2407 3853 4293
Size: 2407 1446 440
Fragments arranged by size:
2407 1446 440
SphI G_CATG'C
Cuts at: 0 1874 2448 4293
Size: 1874 574 1845
Fragments arranged by size:
1874 1845 574
SspI AAT'ATT
Cuts at: 0 543 1441 4293
Size: 543 898 2852
Fragments arranged by size:
2852 898 543
StuI AGG'CCT
Cuts at: 0 868 1302 2095 3966 4293
Size: 868 434 793 1871 327
Fragments arranged by size:
1871 868 793 434 327
StyI C'CwwG_G
Cuts at: 0 155 170 440 450 617 702 899 1083
Size: 155 15 270 10 167 85 197 184
Cuts at: 1083 1288 1580 1895 3666 4293
Size: 205 292 315 1771 627
Fragments arranged by size:
1771 627 315 292 270 205 197 184
167 155 85 15 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 700 715 1605 2247 2301 2631 4182 4242
Size: 700 15 890 642 54 330 1551 60
Cuts at: 4242 4293
Size: 51
Fragments arranged by size:
1551 890 700 642 330 60 54 51
15
TatI w'GTAC_w
Cuts at: 0 1772 2583 2956 4293
Size: 1772 811 373 1337
Fragments arranged by size:
1772 1337 811 373
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 14 46 4293
Size: 14 32 4247
Fragments arranged by size:
4247 32 14
Tth111I GACn'n_nGTC
Cuts at: 0 121 4293
Size: 121 4172
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 162 466 2002 4293
Size: 162 304 1536 2291
Fragments arranged by size:
2291 1536 304 162
XmaI C'CCGG_G
Cuts at: 0 60 1214 4293
Size: 60 1154 3079
Fragments arranged by size:
3079 1154 60
Enzymes that do cut:
AarI Acc65I AcuI AfeI AgeI AlwNI ApaI ApaLI
ApoI AvaI AvrII BamHI BanI BanII BbsI BbvCI
BciVI BfrBI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaBI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrDI BsrFI
BssSI BstAPI BstEII BstXI BstYI BtgI BtgZI BtsI
CspCI DraI EaeI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRV FalI FspI HaeII HincII HindIII KpnI MfeI
MmeI MscI MslI MspA1I NcoI NsiI NspI PasI
PflMI PpuMI PspOMI PstI PvuII SacI SanDI SapI
ScaI SfcI SmaI SmlI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AatII AccI AclI AflII AflIII AhdI AleI AlfI
AloI AscI AseI AsiSI BaeI BbeI BcgI BclI
BmgBI BmtI BsaAI BsaHI BsiEI BsiWI BsmBI BspEI
BspHI BsrBI BsrGI BssHII BstBI BstZ17I Bsu36I ClaI
DraIII DrdI EagI EcoNI EcoRI FseI FspAI Hin4I
HpaI KasI MluI NaeI NarI NdeI NgoMIV NheI
NotI NruI PacI PciI PfoI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI
SbfI SexAI SfiI SfoI SgfI SgrAI SnaBI SrfI
SwaI XbaI XhoI XmnI ZraI