Restriction map for
pFN21AA1018
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# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII Acc65I AccII AclI AflII
AhdI AocI Aor51HI AscI AseI
AsnI Asp700I Asp718I AspEI AviII
AvrII BbeI BbvCI BcgI BciVI
BclI BfrI BglII BlnI BlpI
BpmI Bpu1102I BsaBI BsaI BsiWI
Bsp1407I BspDI BsrBI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstZ17I Bsu36I CelII ClaI
CpoI DrdI Eam1105I Eco47III Eco81I
EcoNI EcoO65I EcoRI EcoT22I FbaI
FseI FspI HpaI KasI KpnI
KspI MamI MfeI MluI MunI
MvnI NaeI NarI NcoI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PmeI PpuMI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SapI SbfI ScaI SfoI
SfuI SgrAI SpeI SphI Sse8387I
SspBI SspI SwaI XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AccI 828 GTCTAC GT^[AC][GT]AC
AgeI 1108 ACCGGT A^CCGGT
Alw44I 2610 GTGCAC G^TGCAC
ApaI 2808 GGGCCC GGGCC^C
ApaLI 2610 GTGCAC G^TGCAC
AspI 2855 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BsiEI 1737 CGGCCG CG[GA][CT]^CG
BsmBI 2768 CGTCTC CGTCTC(1/5)
BspHI 2670 TCATGA T^CATGA
BspLU11I 2042 ACATGT A^CATGT
BstXI 1580 CCAAAGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 2363 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 2423 CCACGG C^C[GA][CT]GG
EagI 1737 CGGCCG C^GGCCG
EclXI 1737 CGGCCG C^GGCCG
Eco52I 1737 CGGCCG C^GGCCG
EcoRV 1048 GATATC GAT^ATC
EcoT14I 1586 CCTTGG C^C[AT][AT]GG
HaeII 2159 AGCGCC [GA]GCGC^[CT]
HindIII 45 AAGCTT A^AGCTT
NdeI 685 CATATG CA^TATG
PflFI 2855 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2362 CCACAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1108 ACCGGT A^CCGGT
RcaI 2670 TCATGA T^CATGA
SacI 1885 GAGCTC GAGCT^C
SexAI 2879 ACCTGGT A^CC[AT]GGT
SfiI 2400 GGCCGGGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 734 CCCGGG CCC^GGG
SnaBI 487 TACGTA TAC^GTA
StuI 34 AGGCCT AGG^CCT
StyI 1586 CCTTGG C^C[AT][AT]GG
Tth111I 2855 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2362 CCACAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2484 CCAGGGGATTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 734 CCCGGG C^CCGGG
XmaI 734 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccIII 2205 TCCGGA T^CCGGA
2565 TCCGGA
AfaI 1156 GTAC GT^AC
1603 GTAC
AvaI 734 CCCGGG C^[CT]CG[GA]G
2680 CCCGAG
BamHI 216 GGATCC G^GATCC
2202 GGATCC
BanII 1885 GAGCTC G[GA]GC[CT]^C
2808 GGGCCC
BbrPI 2335 CACGTG CAC^GTG
2712 CACGTG
BseAI 2205 TCCGGA T^CCGGA
2565 TCCGGA
BsoBI 734 CCCGGG C^[CT]CG[GA]G
2680 CCCGAG
BspEI 2205 TCCGGA T^CCGGA
2565 TCCGGA
BspMI 114 ACCTGC ACCTGC(4/8)
225 GCAGGT
BsrDI 1088 GCAATG GCAATG(2/0)
1852 GCAATG
CfoI 2160 GCGC GCG^C
2253 GCGC
FauI 1637 CCCGC CCCGC(4/6)
2694 GCGGG
HhaI 2160 GCGC GCG^C
2253 GCGC
HinP1I 2160 GCGC G^CGC
2253 GCGC
HincII 1659 GTTGAC GT[CT]^[GA]AC
1729 GTCAAC
HindII 1659 GTTGAC GT[CT]^[GA]AC
1729 GTCAAC
MroI 2205 TCCGGA T^CCGGA
2565 TCCGGA
PleI 1666 GACTC GAGTC(4/5)
2263 GAGTC
PmaCI 2335 CACGTG CAC^GTG
2712 CACGTG
PmlI 2335 CACGTG CAC^GTG
2712 CACGTG
RsaI 1156 GTAC GT^AC
1603 GTAC
SmlI 1159 CTTGAG C^T[CT][GA]AG
2647 CTTGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AciI AcsI AcyI AflIII AluI
AlwI AlwNI ApoI AspHI AvaII
BalI BanI BbsI BbvI BcnI
BfaI BglI BmyI BpuAI BsaAI
BsaHI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstYI Cac8I
Cfr10I Cfr13I DdeI DpnII DraI
DraII EaeI EarI EcoO109I EcoRII
Fnu4HI FokI HaeIII HapII HgaI
Hin1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PstI PvuII Sau3AI Sau96I
ScrFI SfaNI SfcI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII