(Circular) (Six-base) MAPSORT of: pFN21AA1018.seq Check: 7526 from: 1 to: 7756 pFN21AA1018 7756 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2128 2128 Size: 7756 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5566 278 Size: 53 83 186 936 4030 2468 Fragments arranged by size: 4030 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2834 5099 1937 Size: 897 2265 4594 Fragments arranged by size: 4594 2265 897 Acc65I G'GTAC_C Cuts at: 5077 5077 Size: 7756 AclI AA'CG_TT Cuts at: 1563 5883 6256 1563 Size: 4320 373 3063 Fragments arranged by size: 4320 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2577 2823 2916 3423 4501 4564 4763 5022 Size: 945 246 93 507 1078 63 199 259 Cuts at: 5022 5819 7203 7471 1632 Size: 797 1384 268 1917 Fragments arranged by size: 1917 1384 1078 945 797 507 268 259 246 199 93 63 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7559 AflIII A'CryG_T Cuts at: 1203 2646 4047 4339 6655 7632 1203 Size: 1443 1401 292 2316 977 1327 Fragments arranged by size: 2316 1443 1401 1327 977 292 AgeI A'CCGG_T Cuts at: 3113 6587 3113 Size: 3474 4282 AhdI GACnn_n'nnGTC Cuts at: 6485 6485 Size: 7756 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7756 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4863 1184 Size: 3679 4077 AlwNI CAG_nnn'CTG Cuts at: 3242 3383 3490 4993 5499 7071 3242 Size: 141 107 1503 506 1572 3927 Fragments arranged by size: 3927 1572 1503 506 141 107 ApaI G_GGCC'C Cuts at: 4817 4817 Size: 7756 ApaLI G'TGCA_C Cuts at: 4615 5813 6969 4615 Size: 1198 1156 5402 Fragments arranged by size: 5402 1198 1156 ApoI r'AATT_y Cuts at: 1493 2245 2326 2528 2616 3221 4095 5065 5291 Size: 752 81 202 88 605 874 970 226 Cuts at: 5291 5443 7552 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 970 874 752 605 226 202 152 88 81 AseI AT'TA_AT Cuts at: 160 1032 6310 160 Size: 872 5278 1606 Fragments arranged by size: 5278 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7756 AvaI C'yCGr_G Cuts at: 1870 1943 2739 4685 5081 1870 Size: 73 796 1946 396 4545 Fragments arranged by size: 4545 1946 796 396 73 BamHI G'GATC_C Cuts at: 2221 4207 5086 5453 2221 Size: 1986 879 367 4524 Fragments arranged by size: 4524 1986 879 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2155 2178 4839 5077 6532 Size: 325 217 627 368 23 2661 238 1455 Cuts at: 6532 618 Size: 1842 Fragments arranged by size: 2661 1842 1455 627 368 325 238 217 23 BanII G_rGCy'C Cuts at: 1391 3894 4817 5075 1391 Size: 2503 923 258 4072 Fragments arranged by size: 4072 2503 923 258 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2850 3693 4570 928 Size: 971 951 843 877 4114 Fragments arranged by size: 4114 971 951 877 843 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5947 5981 1903 Size: 34 4010 34 3678 Fragments arranged by size: 4010 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5648 6858 5648 Size: 1210 6546 BclI T'GATC_A Cuts at: 1548 1548 Size: 7756 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4412 4595 4655 Size: 107 122 71 631 605 2740 183 60 Cuts at: 4655 4709 4812 6367 136 Size: 54 103 1555 1525 Fragments arranged by size: 2740 1555 1525 631 605 183 122 107 103 71 60 54 BglII A'GATC_T Cuts at: 7751 7751 Size: 7756 BlpI GC'TnA_GC Cuts at: 5173 5173 Size: 7756 Bme1580I G_kGCm'C Cuts at: 1790 4367 4619 4817 5817 6973 1790 Size: 2577 252 198 1000 1156 2573 Fragments arranged by size: 2577 2573 1156 1000 252 198 BmgBI CAC'GTC Cuts at: 1318 4773 1318 Size: 3455 4301 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3883 5487 6445 Size: 416 53 83 399 24 2466 1604 958 Cuts at: 6445 442 Size: 1753 Fragments arranged by size: 2466 1753 1604 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7756 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4892 4892 Size: 7756 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 6416 1239 Size: 5177 2579 Bpu10I CC'TnA_GC Cuts at: 3885 4788 5051 3885 Size: 903 263 6590 Fragments arranged by size: 6590 903 263 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3185 4673 5881 6746 7044 3185 Size: 1488 1208 865 298 3897 Fragments arranged by size: 3897 1488 1208 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6419 882 Size: 617 4920 2219 Fragments arranged by size: 4920 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2494 4342 4719 5547 493 Size: 711 1290 1848 377 828 2702 Fragments arranged by size: 2702 1848 1290 828 711 377 BsaBI GATnn'nnATC Cuts at: 7750 7750 Size: 7756 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3498 3690 4642 5563 Size: 53 83 186 936 1965 192 952 921 Cuts at: 5563 5945 6580 275 Size: 382 635 1451 Fragments arranged by size: 1965 1451 952 936 921 635 382 192 186 83 53 BsaWI w'CCGG_w Cuts at: 3113 4210 4570 6189 6587 6861 7008 7419 3113 Size: 1097 360 1619 398 274 147 411 3450 Fragments arranged by size: 3450 1619 1097 411 398 360 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3068 3098 1178 Size: 30 165 30 1665 30 5836 Fragments arranged by size: 5836 1665 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3012 4463 4526 4703 1186 Size: 1826 1451 63 177 4239 Fragments arranged by size: 4239 1826 1451 177 63 BseYI C'CCAG_C Cuts at: 4645 4758 4993 6959 7455 4645 Size: 113 235 1966 496 4946 Fragments arranged by size: 4946 1966 496 235 113 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3517 3585 4855 1163 Size: 573 1781 68 1270 4064 Fragments arranged by size: 4064 1781 1270 573 68 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3745 5206 5967 6995 1540 Size: 275 186 1744 1461 761 1028 2301 Fragments arranged by size: 2301 1744 1461 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3894 4442 4619 4824 5817 5902 6973 3894 Size: 548 177 205 993 85 1071 4677 Fragments arranged by size: 4677 1071 993 548 205 177 85 BsmI GAATG_Cn' Cuts at: 2679 3066 3364 4598 5267 5360 2679 Size: 387 298 1234 669 93 5075 Fragments arranged by size: 5075 1234 669 387 298 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4779 4779 Size: 7756 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3894 4367 4442 4619 4817 4824 5075 Size: 399 2104 473 75 177 198 7 251 Cuts at: 5075 5817 5902 6973 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 2104 1071 742 473 399 251 198 177 85 75 7 BspEI T'CCGG_A Cuts at: 4210 4570 7419 4210 Size: 360 2849 4547 Fragments arranged by size: 4547 2849 360 BspHI T'CATG_A Cuts at: 4675 5645 7375 4675 Size: 970 1730 5056 Fragments arranged by size: 5056 1730 970 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2128 2221 5111 844 Size: 1284 93 2890 3489 Fragments arranged by size: 3489 2890 1284 93 BsrBI CCG'CTC Cuts at: 1394 1823 5643 1394 Size: 429 3820 3507 Fragments arranged by size: 3820 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3100 3864 6251 6425 65 Size: 1183 1852 764 2387 174 1396 Fragments arranged by size: 2387 1852 1396 1183 764 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2175 2534 3113 6400 6587 1700 Size: 110 365 359 579 3287 187 2869 Fragments arranged by size: 3287 2869 579 365 359 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7756 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7756 BssSI C'ACGA_G Cuts at: 5816 6828 5816 Size: 1012 6744 BstAPI GCAn_nnn'nTGC Cuts at: 794 2120 2990 4362 794 Size: 1326 870 1372 4188 Fragments arranged by size: 4188 1372 1326 870 BstBI TT'CG_AA Cuts at: 6560 6560 Size: 7756 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7756 BstXI CCAn_nnnn'nTGG Cuts at: 3592 3592 Size: 7756 BstYI r'GATC_y Cuts at: 1972 2221 4207 4792 5086 5453 5838 5855 7307 Size: 249 1986 585 294 367 385 17 1452 Cuts at: 7307 7393 7405 7751 1972 Size: 86 12 346 1977 Fragments arranged by size: 1986 1977 1452 585 385 367 346 294 249 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 4428 513 Size: 552 3363 3841 Fragments arranged by size: 3841 3363 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7017 Fragments arranged by size: 7017 683 56 BtsI GCAGTG_nn' Cuts at: 3573 5272 6066 6086 3573 Size: 1699 794 20 5243 Fragments arranged by size: 5243 1699 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7756 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7721 DraI TTT'AAA Cuts at: 2392 2653 3576 5060 2392 Size: 261 923 1484 5088 Fragments arranged by size: 5088 1484 923 261 DraIII CAC_nnn'GTG Cuts at: 4373 4373 Size: 7756 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6763 809 Size: 507 5447 1802 Fragments arranged by size: 5447 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2338 3513 3742 Size: 54 1425 50 275 18 508 1175 229 Cuts at: 3742 3861 5203 6092 7721 8 Size: 119 1342 889 1629 43 Fragments arranged by size: 1629 1425 1342 1175 889 508 275 229 119 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3742 5203 1537 Size: 275 1930 1461 4090 Fragments arranged by size: 4090 1930 1461 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2596 2667 3309 3974 4304 4782 5686 1353 Size: 1243 71 642 665 330 478 904 3423 Fragments arranged by size: 3423 1243 904 665 642 478 330 71 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4220 6343 6717 6863 387 Size: 1064 138 2631 2123 374 146 1280 Fragments arranged by size: 2631 2123 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3892 3892 Size: 7756 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2577 2823 2916 3423 4501 4564 4763 Size: 393 945 246 93 507 1078 63 199 Cuts at: 4763 5022 5819 6416 7203 7471 1239 Size: 259 797 597 787 268 1524 Fragments arranged by size: 1524 1078 945 797 787 597 507 393 268 259 246 199 93 63 EcoO109I rG'GnC_Cy Cuts at: 3623 4813 4814 4873 4937 3623 Size: 1190 1 59 64 6442 Fragments arranged by size: 6442 1190 64 59 1 EcoRI G'AATT_C Cuts at: 5065 5443 5065 Size: 378 7378 EcoRV GAT'ATC Cuts at: 3055 3055 Size: 7756 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2867 4315 2867 Size: 1448 6308 FspI TGC'GCA Cuts at: 6262 6262 Size: 7756 HaeII r_GCGC'y Cuts at: 1678 4168 6903 1678 Size: 2490 2735 2531 Fragments arranged by size: 2735 2531 2490 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2954 2986 3068 3100 4000 Size: 32 135 32 1382 32 82 32 900 Cuts at: 4000 4032 4784 4816 6397 6429 6471 6503 1373 Size: 32 752 32 1581 32 42 32 2626 Fragments arranged by size: 2626 1581 1382 900 752 135 82 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3666 3736 5100 5346 669 Size: 988 281 1728 70 1364 246 3079 Fragments arranged by size: 3079 1728 1364 988 281 246 70 HindIII A'AGCT_T Cuts at: 748 2050 748 Size: 1302 6454 HpaI GTT'AAC Cuts at: 5346 5346 Size: 7756 KpnI G_GTAC'C Cuts at: 5081 5081 Size: 7756 MfeI C'AATT_G Cuts at: 5355 5355 Size: 7756 MluI A'CGCG_T Cuts at: 7632 7632 Size: 7756 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5011 6870 7054 1305 Size: 3706 1859 184 2007 Fragments arranged by size: 3706 2007 1859 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2340 3515 3863 10 Size: 54 1425 343 508 1175 348 3903 Fragments arranged by size: 3903 1425 1175 508 348 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2184 3833 4050 4556 5714 6073 6232 Size: 648 1018 1649 217 506 1158 359 159 Cuts at: 6232 518 Size: 2042 Fragments arranged by size: 2042 1649 1158 1018 648 506 359 217 159 MspA1I CmG'CkG Cuts at: 1606 2950 3490 4418 4479 4669 4758 5172 5849 Size: 1344 540 928 61 190 89 414 677 Cuts at: 5849 6997 7242 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1344 1148 928 677 540 414 245 190 89 61 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 7204 NdeI CA'TA_TG Cuts at: 387 2691 387 Size: 2304 5452 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7756 NotI GC'GGCC_GC Cuts at: 5203 5203 Size: 7756 NspI r_CATG'y Cuts at: 1132 1229 2901 3838 4051 5114 6659 1132 Size: 97 1672 937 213 1063 1545 2229 Fragments arranged by size: 2229 1672 1545 1063 937 213 97 PasI CC'CwG_GG Cuts at: 1348 3627 1348 Size: 2279 5477 PciI A'CATG_T Cuts at: 4047 6655 4047 Size: 2608 5148 PflMI CCAn_nnn'nTGG Cuts at: 1108 4373 1108 Size: 3265 4491 PmeI GTTT'AAAC Cuts at: 5060 5060 Size: 7756 PmlI CAC'GTG Cuts at: 4342 4719 4342 Size: 377 7379 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5825 5857 7338 7370 5825 Size: 32 1481 32 6211 Fragments arranged by size: 6211 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7756 PsiI TTA'TAA Cuts at: 5326 5326 Size: 7756 PspOMI G'GGCC_C Cuts at: 4813 4813 Size: 7756 PstI C_TGCA'G Cuts at: 830 2986 3030 4038 4668 4989 5108 830 Size: 2156 44 1008 630 321 119 3478 Fragments arranged by size: 3478 2156 1008 630 321 119 44 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7756 PvuII CAG'CTG Cuts at: 2950 3490 4669 4758 2950 Size: 540 1179 89 5948 Fragments arranged by size: 5948 1179 540 89 SacI G_AGCT'C Cuts at: 3894 3894 Size: 7756 SalI G'TCGA_C Cuts at: 1936 5098 1936 Size: 3162 4594 SbfI CC_TGCA'GG Cuts at: 5108 5108 Size: 7756 ScaI AGT'ACT Cuts at: 6004 7716 6004 Size: 1712 6044 SexAI A'CCwGG_T Cuts at: 4884 4884 Size: 7756 SfcI C'TryA_G Cuts at: 826 1045 2982 3026 3371 4034 4664 4985 5104 Size: 219 1937 44 345 663 630 321 119 Cuts at: 5104 6239 6920 7111 826 Size: 1135 681 191 1471 Fragments arranged by size: 1937 1471 1135 681 663 630 345 321 219 191 119 44 SfiI GGCCn_nnn'nGGCC Cuts at: 4412 4412 Size: 7756 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7756 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7756 SmaI CCC'GGG Cuts at: 1872 2741 5083 1872 Size: 869 2342 4545 Fragments arranged by size: 4545 2342 869 SmlI C'TyrA_G Cuts at: 820 1017 1943 3164 4652 5860 6761 7023 820 Size: 197 926 1221 1488 1208 901 262 1553 Fragments arranged by size: 1553 1488 1221 1208 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2494 5547 493 Size: 2001 3053 2702 Fragments arranged by size: 3053 2702 2001 SpeI A'CTAG_T Cuts at: 152 152 Size: 7756 SphI G_CATG'C Cuts at: 1132 5114 1132 Size: 3982 3774 SspI AAT'ATT Cuts at: 5 52 5680 5 Size: 47 5628 2081 Fragments arranged by size: 5628 2081 47 StuI AGG'CCT Cuts at: 1517 2041 1517 Size: 524 7232 StyI C'CwwG_G Cuts at: 513 1065 3591 5195 513 Size: 552 2526 1604 3074 Fragments arranged by size: 3074 2526 1604 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3683 3981 4696 5783 5800 5953 6138 Size: 1255 2103 298 715 1087 17 153 185 Cuts at: 6138 6583 325 Size: 445 1498 Fragments arranged by size: 2103 1498 1255 1087 715 445 298 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 6002 7714 96 Size: 274 80 33 51 1443 4025 1712 138 Fragments arranged by size: 4025 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7716 7748 7716 Size: 32 7724 Tth111I GACn'n_nGTC Cuts at: 1316 4863 1316 Size: 3547 4209 XbaI T'CTAG_A Cuts at: 5092 5092 Size: 7756 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4496 4496 Size: 7756 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7756 XmaI C'CCGG_G Cuts at: 1870 2739 5081 1870 Size: 869 2342 4545 Fragments arranged by size: 4545 2342 869 XmnI GAAnn'nnTTC Cuts at: 5885 5885 Size: 7756 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5564 276 Size: 53 83 186 936 4030 2468 Fragments arranged by size: 4030 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BstZ17I Bsu36I EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspXI PsrI RsrII SacII SanDI SapI SfoI SrfI SwaI