Restriction map for
pFN21AA1018
(Linear) (Six-base) MAPSORT of: KIAA1018.seq Check: 6902 from: 1 to: 3051
KIAA1018.orf
With 182 enzymes: SgfI *
February 28, 2007 11:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 123 3051
Size: 123 2928
AccI GT'mk_AC
Cuts at: 0 829 3051
Size: 829 2222
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 572 818 911 1418 2496 2559 2758 3017
Size: 572 246 93 507 1078 63 199 259
Cuts at: 3017 3051
Size: 34
Fragments arranged by size:
1078 572 507 259 246 199 93 63
34
AflIII A'CryG_T
Cuts at: 0 641 2042 2334 3051
Size: 641 1401 292 717
Fragments arranged by size:
1401 717 641 292
AgeI A'CCGG_T
Cuts at: 0 1108 3051
Size: 1108 1943
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2858 3051
Size: 2858 193
AlwNI CAG_nnn'CTG
Cuts at: 0 1237 1378 1485 2988 3051
Size: 1237 141 107 1503 63
Fragments arranged by size:
1503 1237 141 107 63
ApaI G_GGCC'C
Cuts at: 0 2812 3051
Size: 2812 239
ApaLI G'TGCA_C
Cuts at: 0 2610 3051
Size: 2610 441
ApoI r'AATT_y
Cuts at: 0 240 321 523 611 1216 2090 3051
Size: 240 81 202 88 605 874 961
Fragments arranged by size:
961 874 605 240 202 88 81
AvaI C'yCGr_G
Cuts at: 0 734 2680 3051
Size: 734 1946 371
Fragments arranged by size:
1946 734 371
BamHI G'GATC_C
Cuts at: 0 216 2202 3051
Size: 216 1986 849
Fragments arranged by size:
1986 849 216
BanI G'GyrC_C
Cuts at: 0 150 173 2834 3051
Size: 150 23 2661 217
Fragments arranged by size:
2661 217 150 23
BanII G_rGCy'C
Cuts at: 0 1889 2812 3051
Size: 1889 923 239
Fragments arranged by size:
1889 923 239
BbsI GAAGACnn'nnnn_
Cuts at: 0 845 1688 2565 3051
Size: 845 843 877 486
Fragments arranged by size:
877 845 843 486
BglI GCCn_nnn'nGGC
Cuts at: 0 2407 2590 2650 2704 2807 3051
Size: 2407 183 60 54 103 244
Fragments arranged by size:
2407 244 183 103 60 54
Bme1580I G_kGCm'C
Cuts at: 0 2362 2614 2812 3051
Size: 2362 252 198 239
Fragments arranged by size:
2362 252 239 198
BmgBI CAC'GTC
Cuts at: 0 2768 3051
Size: 2768 283
BmrI ACTGGGnnnn_n'
Cuts at: 0 1878 3051
Size: 1878 1173
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2887 3051
Size: 2887 164
Bpu10I CC'TnA_GC
Cuts at: 0 1880 2783 3046 3051
Size: 1880 903 263 5
Fragments arranged by size:
1880 903 263 5
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1180 2668 3051
Size: 1180 1488 383
Fragments arranged by size:
1488 1180 383
BsaAI yAC'GTr
Cuts at: 0 489 2337 2714 3051
Size: 489 1848 377 337
Fragments arranged by size:
1848 489 377 337
BsaHI Gr'CG_yC
Cuts at: 0 1493 1685 2637 3051
Size: 1493 192 952 414
Fragments arranged by size:
1493 952 414 192
BsaWI w'CCGG_w
Cuts at: 0 1108 2205 2565 3051
Size: 1108 1097 360 486
Fragments arranged by size:
1108 1097 486 360
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1063 1093 3051
Size: 1063 30 1958
Fragments arranged by size:
1958 1063 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1007 2458 2521 2698 3051
Size: 1007 1451 63 177 353
Fragments arranged by size:
1451 1007 353 177 63
BseYI C'CCAG_C
Cuts at: 0 2640 2753 2988 3051
Size: 2640 113 235 63
Fragments arranged by size:
2640 235 113 63
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1512 1580 2850 3051
Size: 1512 68 1270 201
Fragments arranged by size:
1512 1270 201 68
BsiEI CG_ry'CG
Cuts at: 0 1740 3051
Size: 1740 1311
BsiHKAI G_wGCw'C
Cuts at: 0 1889 2437 2614 2819 3051
Size: 1889 548 177 205 232
Fragments arranged by size:
1889 548 232 205 177
BsmI GAATG_Cn'
Cuts at: 0 674 1061 1359 2593 3051
Size: 674 387 298 1234 458
Fragments arranged by size:
1234 674 458 387 298
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2774 3051
Size: 2774 277
Bsp1286I G_dGCh'C
Cuts at: 0 1889 2362 2437 2614 2812 2819 3051
Size: 1889 473 75 177 198 7 232
Fragments arranged by size:
1889 473 232 198 177 75 7
BspEI T'CCGG_A
Cuts at: 0 2205 2565 3051
Size: 2205 360 486
Fragments arranged by size:
2205 486 360
BspHI T'CATG_A
Cuts at: 0 2670 3051
Size: 2670 381
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 123 216 3051
Size: 123 93 2835
Fragments arranged by size:
2835 123 93
BsrDI GCAATG_nn'
Cuts at: 0 1095 1859 3051
Size: 1095 764 1192
Fragments arranged by size:
1192 1095 764
BsrFI r'CCGG_y
Cuts at: 0 170 529 1108 3051
Size: 170 359 579 1943
Fragments arranged by size:
1943 579 359 170
BstAPI GCAn_nnn'nTGC
Cuts at: 0 115 985 2357 3051
Size: 115 870 1372 694
Fragments arranged by size:
1372 870 694 115
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1587 3051
Size: 1587 1464
BstYI r'GATC_y
Cuts at: 0 216 2202 2787 3051
Size: 216 1986 585 264
Fragments arranged by size:
1986 585 264 216
BtgI C'CryG_G
Cuts at: 0 2423 3051
Size: 2423 628
BtsI GCAGTG_nn'
Cuts at: 0 1568 3051
Size: 1568 1483
DraI TTT'AAA
Cuts at: 0 387 648 1571 3051
Size: 387 261 923 1480
Fragments arranged by size:
1480 923 387 261
DraIII CAC_nnn'GTG
Cuts at: 0 2368 3051
Size: 2368 683
EaeI y'GGCC_r
Cuts at: 0 333 1508 1737 1856 3051
Size: 333 1175 229 119 1195
Fragments arranged by size:
1195 1175 333 229 119
EagI C'GGCC_G
Cuts at: 0 1737 3051
Size: 1737 1314
EarI CTCTTCn'nnn_
Cuts at: 0 591 662 1304 1969 2299 2777 3051
Size: 591 71 642 665 330 478 274
Fragments arranged by size:
665 642 591 478 330 274 71
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2215 3051
Size: 2215 836
EcoICRI GAG'CTC
Cuts at: 0 1887 3051
Size: 1887 1164
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 572 818 911 1418 2496 2559 2758 3017
Size: 572 246 93 507 1078 63 199 259
Cuts at: 3017 3051
Size: 34
Fragments arranged by size:
1078 572 507 259 246 199 93 63
34
EcoO109I rG'GnC_Cy
Cuts at: 0 1618 2808 2809 2868 2932 3051
Size: 1618 1190 1 59 64 119
Fragments arranged by size:
1618 1190 119 64 59 1
EcoRV GAT'ATC
Cuts at: 0 1050 3051
Size: 1050 2001
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 862 2310 3051
Size: 862 1448 741
Fragments arranged by size:
1448 862 741
HaeII r_GCGC'y
Cuts at: 0 2163 3051
Size: 2163 888
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 949 981 1063 1095 1995 2027 2779 2811
Size: 949 32 82 32 900 32 752 32
Cuts at: 2811 3051
Size: 240
Fragments arranged by size:
949 900 752 240 82 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1661 1731 3051
Size: 1661 70 1320
Fragments arranged by size:
1661 1320 70
HindIII A'AGCT_T
Cuts at: 0 45 3051
Size: 45 3006
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 3006 3051
Size: 3006 45
MscI TGG'CCA
Cuts at: 0 335 1510 1858 3051
Size: 335 1175 348 1193
Fragments arranged by size:
1193 1175 348 335
MslI CAynn'nnrTG
Cuts at: 0 179 1828 2045 2551 3051
Size: 179 1649 217 506 500
Fragments arranged by size:
1649 506 500 217 179
MspA1I CmG'CkG
Cuts at: 0 945 1485 2413 2474 2664 2753 3051
Size: 945 540 928 61 190 89 298
Fragments arranged by size:
945 928 540 298 190 89 61
NdeI CA'TA_TG
Cuts at: 0 686 3051
Size: 686 2365
NspI r_CATG'y
Cuts at: 0 896 1833 2046 3051
Size: 896 937 213 1005
Fragments arranged by size:
1005 937 896 213
PasI CC'CwG_GG
Cuts at: 0 1622 3051
Size: 1622 1429
PciI A'CATG_T
Cuts at: 0 2042 3051
Size: 2042 1009
PflMI CCAn_nnn'nTGG
Cuts at: 0 2368 3051
Size: 2368 683
PmlI CAC'GTG
Cuts at: 0 2337 2714 3051
Size: 2337 377 337
Fragments arranged by size:
2337 377 337
PspOMI G'GGCC_C
Cuts at: 0 2808 3051
Size: 2808 243
PstI C_TGCA'G
Cuts at: 0 981 1025 2033 2663 2984 3051
Size: 981 44 1008 630 321 67
Fragments arranged by size:
1008 981 630 321 67 44
PvuII CAG'CTG
Cuts at: 0 945 1485 2664 2753 3051
Size: 945 540 1179 89 298
Fragments arranged by size:
1179 945 540 298 89
SacI G_AGCT'C
Cuts at: 0 1889 3051
Size: 1889 1162
SexAI A'CCwGG_T
Cuts at: 0 2879 3051
Size: 2879 172
SfcI C'TryA_G
Cuts at: 0 977 1021 1366 2029 2659 2980 3051
Size: 977 44 345 663 630 321 71
Fragments arranged by size:
977 663 630 345 321 71 44
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2407 3051
Size: 2407 644
SmaI CCC'GGG
Cuts at: 0 736 3051
Size: 736 2315
SmlI C'TyrA_G
Cuts at: 0 1159 2647 3051
Size: 1159 1488 404
Fragments arranged by size:
1488 1159 404
SnaBI TAC'GTA
Cuts at: 0 489 3051
Size: 489 2562
StuI AGG'CCT
Cuts at: 0 36 3051
Size: 36 3015
StyI C'CwwG_G
Cuts at: 0 1586 3051
Size: 1586 1465
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1678 1976 2691 3051
Size: 1678 298 715 360
Fragments arranged by size:
1678 715 360 298
Tth111I GACn'n_nGTC
Cuts at: 0 2858 3051
Size: 2858 193
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2491 3051
Size: 2491 560
XmaI C'CCGG_G
Cuts at: 0 734 3051
Size: 734 2317
Enzymes that do cut:
AarI AccI AcuI AflIII AgeI AlfI AlwNI ApaI
ApaLI ApoI AvaI BamHI BanI BanII BbsI BglI
Bme1580I BmgBI BmrI BplI Bpu10I BpuEI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrDI BsrFI BstAPI
BstXI BstYI BtgI BtsI DraI DraIII EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRV FalI HaeII
Hin4I HincII HindIII MmeI MscI MslI MspA1I NdeI
NspI PasI PciI PflMI PmlI PspOMI PstI PvuII
SacI SexAI SfcI SfiI SmaI SmlI SnaBI StuI
StyI TaqII Tth111I XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AhdI AleI AloI
AscI AseI AsiSI AvrII BaeI BbeI BbvCI BcgI
BciVI BclI BfrBI BglII BlpI BmtI BpmI BsaI
BsaBI BsiWI BsrBI BsrGI BssHII BssSI BstBI BstEII
BstZ17I Bsu36I BtgZI ClaI CspCI DrdI EcoNI EcoRI
FseI FspI FspAI HpaI KasI KpnI MfeI MluI
NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI
PacI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI
PsrI PvuI RsrII SacII SalI SanDI SapI SbfI
ScaI SfoI SgfI SgrAI SpeI SphI SrfI SspI
SwaI TatI TstI XbaI XhoI XmnI ZraI