(Circular) (Six-base) MAPSORT of: pFN21AA1008.seq Check: 4538 from: 1 to: 7489 pFN21AA1008 7489 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3046 3046 Size: 7489 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5299 278 Size: 53 83 186 936 3763 2468 Fragments arranged by size: 3763 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3348 4832 1937 Size: 1411 1484 4594 Fragments arranged by size: 4594 1484 1411 Acc65I G'GTAC_C Cuts at: 4810 4810 Size: 7489 AclI AA'CG_TT Cuts at: 1563 5616 5989 1563 Size: 4053 373 3063 Fragments arranged by size: 4053 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2580 3708 3794 3840 5552 6936 7204 1632 Size: 948 1128 86 46 1712 1384 268 1917 Fragments arranged by size: 1917 1712 1384 1128 948 268 86 46 AflII C'TTAA_G Cuts at: 820 1017 2614 2903 820 Size: 197 1597 289 5406 Fragments arranged by size: 5406 1597 289 197 AflIII A'CryG_T Cuts at: 1203 6388 7365 1203 Size: 5185 977 1327 Fragments arranged by size: 5185 1327 977 AgeI A'CCGG_T Cuts at: 6320 6320 Size: 7489 AhdI GACnn_n'nnGTC Cuts at: 6218 6218 Size: 7489 AleI CACnn'nnGTG Cuts at: 1166 2216 1166 Size: 1050 6439 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7489 AlwNI CAG_nnn'CTG Cuts at: 2181 5232 6804 2181 Size: 3051 1572 2866 Fragments arranged by size: 3051 2866 1572 ApaLI G'TGCA_C Cuts at: 5546 6702 5546 Size: 1156 6333 ApoI r'AATT_y Cuts at: 1493 3060 3136 3169 3409 3768 3892 3908 3945 Size: 1567 76 33 240 359 124 16 37 Cuts at: 3945 3956 4014 4042 4366 4798 5024 5176 7285 Size: 11 58 28 324 432 226 152 2109 Cuts at: 7285 1493 Size: 1697 Fragments arranged by size: 2109 1697 1567 432 359 324 240 226 152 124 76 58 37 33 28 16 11 AseI AT'TA_AT Cuts at: 160 1032 3660 4502 6043 160 Size: 872 2628 842 1541 1606 Fragments arranged by size: 2628 1606 1541 872 842 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7489 AvaI C'yCGr_G Cuts at: 1870 1943 2037 2105 4814 1870 Size: 73 94 68 2709 4545 Fragments arranged by size: 4545 2709 94 73 68 BamHI G'GATC_C Cuts at: 4819 5186 4819 Size: 367 7122 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4810 6265 618 Size: 325 217 627 3023 1455 1842 Fragments arranged by size: 3023 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 4808 1391 Size: 3417 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3028 3324 928 Size: 971 1129 296 5093 Fragments arranged by size: 5093 1129 971 296 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5680 5714 1903 Size: 34 50 34 3659 34 3678 Fragments arranged by size: 3678 3659 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5381 6591 5381 Size: 1210 6279 BclI T'GATC_A Cuts at: 1548 1548 Size: 7489 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6100 136 Size: 107 122 71 631 605 4428 1525 Fragments arranged by size: 4428 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3862 7484 3862 Size: 3622 3867 BlpI GC'TnA_GC Cuts at: 3545 4213 4906 3545 Size: 668 693 6128 Fragments arranged by size: 6128 693 668 Bme1580I G_kGCm'C Cuts at: 1790 5550 6706 1790 Size: 3760 1156 2573 Fragments arranged by size: 3760 2573 1156 BmgBI CAC'GTC Cuts at: 1318 3577 1318 Size: 2259 5230 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4417 5220 6178 Size: 416 53 83 399 24 3000 803 958 Cuts at: 6178 442 Size: 1753 Fragments arranged by size: 3000 1753 958 803 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2676 1056 Size: 1620 5869 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3072 4606 3072 Size: 1534 5955 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2168 2260 2266 3304 6149 1239 Size: 929 92 6 1038 2845 2579 Fragments arranged by size: 2845 2579 1038 929 92 6 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1994 5614 6479 6777 1994 Size: 3620 865 298 2706 Fragments arranged by size: 3620 2706 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2968 6152 882 Size: 617 1469 3184 2219 Fragments arranged by size: 3184 2219 1469 617 BsaAI yAC'GTr Cuts at: 493 1204 5280 493 Size: 711 4076 2702 Fragments arranged by size: 4076 2702 711 BsaBI GATnn'nnATC Cuts at: 2057 4200 7483 2057 Size: 2143 3283 2063 Fragments arranged by size: 3283 2143 2063 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5296 5678 6313 275 Size: 53 83 186 936 3763 382 635 1451 Fragments arranged by size: 3763 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5922 6320 6594 6741 7152 5922 Size: 398 274 147 411 6259 Fragments arranged by size: 6259 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7264 Fragments arranged by size: 7264 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 7489 BseYI C'CCAG_C Cuts at: 6692 7188 6692 Size: 496 6993 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2210 1163 Size: 573 474 6442 Fragments arranged by size: 6442 573 474 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4939 5700 6728 1540 Size: 275 186 2938 761 1028 2301 Fragments arranged by size: 2938 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2073 5550 5635 6706 2073 Size: 3477 85 1071 2856 Fragments arranged by size: 3477 2856 1071 85 BsmI GAATG_Cn' Cuts at: 2904 4492 4722 5000 5093 2904 Size: 1588 230 278 93 5300 Fragments arranged by size: 5300 1588 278 230 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2076 4224 2076 Size: 2148 5341 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2073 4808 5550 5635 6706 1391 Size: 399 283 2735 742 85 1071 2174 Fragments arranged by size: 2735 2174 1071 742 399 283 85 BspEI T'CCGG_A Cuts at: 7152 7152 Size: 7489 BspHI T'CATG_A Cuts at: 3949 5378 7108 3949 Size: 1429 1730 4330 Fragments arranged by size: 4330 1730 1429 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2085 3046 4844 844 Size: 1241 961 1798 3489 Fragments arranged by size: 3489 1798 1241 961 BsrBI CCG'CTC Cuts at: 1394 1823 5376 1394 Size: 429 3553 3507 Fragments arranged by size: 3553 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5984 6158 65 Size: 1183 4736 174 1396 Fragments arranged by size: 4736 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2140 6133 6320 1700 Size: 110 330 3993 187 2869 Fragments arranged by size: 3993 2869 330 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7489 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7489 BssSI C'ACGA_G Cuts at: 2132 5549 6561 2132 Size: 3417 1012 3060 Fragments arranged by size: 3417 3060 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2077 2181 2200 794 Size: 1283 104 19 6083 Fragments arranged by size: 6083 1283 104 19 BstBI TT'CG_AA Cuts at: 3979 6293 3979 Size: 2314 5175 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7489 BstXI CCAn_nnnn'nTGG Cuts at: 4075 4075 Size: 7489 BstYI r'GATC_y Cuts at: 1972 3862 4680 4819 5186 5571 5588 7040 7126 Size: 1890 818 139 367 385 17 1452 86 Cuts at: 7126 7138 7484 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1890 1452 818 385 367 346 139 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3323 3323 Size: 7489 BtgI C'CryG_G Cuts at: 513 1065 3745 3897 513 Size: 552 2680 152 4105 Fragments arranged by size: 4105 2680 552 152 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6750 Fragments arranged by size: 6750 683 56 BtsI GCAGTG_nn' Cuts at: 5005 5799 5819 5005 Size: 794 20 6675 Fragments arranged by size: 6675 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7489 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7454 DraI TTT'AAA Cuts at: 2763 4793 2763 Size: 2030 5459 DraIII CAC_nnn'GTG Cuts at: 3163 3163 Size: 7489 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6496 809 Size: 507 5180 1802 Fragments arranged by size: 5180 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4936 5825 7454 Size: 54 1425 50 275 18 3106 889 1629 Cuts at: 7454 8 Size: 43 Fragments arranged by size: 3106 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4936 1537 Size: 275 3124 4090 Fragments arranged by size: 4090 3124 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2880 3484 5419 1353 Size: 1527 604 1935 3423 Fragments arranged by size: 3423 1935 1527 604 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6076 6450 6596 387 Size: 1064 138 4487 374 146 1280 Fragments arranged by size: 4487 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2168 2260 2266 2580 3304 3708 3794 Size: 393 536 92 6 314 724 404 86 Cuts at: 3794 3840 5552 6149 6936 7204 1239 Size: 46 1712 597 787 268 1524 Fragments arranged by size: 1712 1524 787 724 597 536 404 393 314 268 92 86 46 6 EcoO109I rG'GnC_Cy Cuts at: 2164 2256 2164 Size: 92 7397 EcoRI G'AATT_C Cuts at: 3060 4798 5176 3060 Size: 1738 378 5373 Fragments arranged by size: 5373 1738 378 EcoRV GAT'ATC Cuts at: 3146 3146 Size: 7489 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2041 2041 Size: 7489 FseI GG_CCGG'CC Cuts at: 2144 2144 Size: 7489 FspI TGC'GCA Cuts at: 2113 5995 2113 Size: 3882 3607 HaeII r_GCGC'y Cuts at: 1678 6636 1678 Size: 4958 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2073 2105 3464 3496 6130 Size: 32 135 32 501 32 1359 32 2634 Cuts at: 6130 6162 6204 6236 1373 Size: 32 42 32 2626 Fragments arranged by size: 2634 2626 1359 501 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4326 4833 5079 669 Size: 988 281 2388 507 246 3079 Fragments arranged by size: 3079 2388 988 507 281 246 HindIII A'AGCT_T Cuts at: 748 4340 4778 748 Size: 3592 438 3459 Fragments arranged by size: 3592 3459 438 HpaI GTT'AAC Cuts at: 5079 5079 Size: 7489 KpnI G_GTAC'C Cuts at: 4814 4814 Size: 7489 MfeI C'AATT_G Cuts at: 5088 5088 Size: 7489 MluI A'CGCG_T Cuts at: 7365 7365 Size: 7489 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3163 6603 6787 1305 Size: 1858 3440 184 2007 Fragments arranged by size: 3440 2007 1858 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5667 Fragments arranged by size: 5667 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2216 3358 3595 4206 5447 5806 5965 Size: 648 1050 1142 237 611 1241 359 159 Cuts at: 5965 518 Size: 2042 Fragments arranged by size: 2042 1241 1142 1050 648 611 359 237 159 MspA1I CmG'CkG Cuts at: 1606 2419 4905 5582 6730 6975 1606 Size: 813 2486 677 1148 245 2120 Fragments arranged by size: 2486 2120 1148 813 677 245 NaeI GCC'GGC Cuts at: 2142 2142 Size: 7489 NcoI C'CATG_G Cuts at: 513 1065 3897 513 Size: 552 2832 4105 Fragments arranged by size: 4105 2832 552 NdeI CA'TA_TG Cuts at: 387 3682 387 Size: 3295 4194 NgoMIV G'CCGG_C Cuts at: 2140 2140 Size: 7489 NheI G'CTAG_C Cuts at: 1052 2672 1052 Size: 1620 5869 NotI GC'GGCC_GC Cuts at: 4936 4936 Size: 7489 NspI r_CATG'y Cuts at: 1132 1229 2992 3971 4847 6392 1132 Size: 97 1763 979 876 1545 2229 Fragments arranged by size: 2229 1763 1545 979 876 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7489 PciI A'CATG_T Cuts at: 6388 6388 Size: 7489 PflMI CCAn_nnn'nTGG Cuts at: 1108 2984 4514 1108 Size: 1876 1530 4083 Fragments arranged by size: 4083 1876 1530 PmeI GTTT'AAAC Cuts at: 4793 4793 Size: 7489 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5558 5590 7071 7103 5558 Size: 32 1481 32 5944 Fragments arranged by size: 5944 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2164 2256 2164 Size: 92 7397 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7489 PsiI TTA'TAA Cuts at: 5059 5059 Size: 7489 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4692 4724 4692 Size: 32 7457 PstI C_TGCA'G Cuts at: 830 3870 4841 830 Size: 3040 971 3478 Fragments arranged by size: 3478 3040 971 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7489 PvuII CAG'CTG Cuts at: 2419 2419 Size: 7489 SalI G'TCGA_C Cuts at: 1936 4831 1936 Size: 2895 4594 SbfI CC_TGCA'GG Cuts at: 4841 4841 Size: 7489 ScaI AGT'ACT Cuts at: 5737 7449 5737 Size: 1712 5777 SfcI C'TryA_G Cuts at: 826 1045 2624 2697 2937 3858 3866 4837 5972 Size: 219 1579 73 240 921 8 971 1135 Cuts at: 5972 6653 6844 826 Size: 681 191 1471 Fragments arranged by size: 1579 1471 1135 971 921 681 240 219 191 73 8 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7489 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7489 SmaI CCC'GGG Cuts at: 1872 2039 2107 4816 1872 Size: 167 68 2709 4545 Fragments arranged by size: 4545 2709 167 68 SmlI C'TyrA_G Cuts at: 820 1017 1943 2009 2614 2903 5593 6494 6756 Size: 197 926 66 605 289 2690 901 262 Cuts at: 6756 820 Size: 1553 Fragments arranged by size: 2690 1553 926 901 605 289 262 197 66 SnaBI TAC'GTA Cuts at: 493 5280 493 Size: 4787 2702 SpeI A'CTAG_T Cuts at: 152 3737 152 Size: 3585 3904 SphI G_CATG'C Cuts at: 1132 2992 4847 1132 Size: 1860 1855 3774 Fragments arranged by size: 3774 1860 1855 SspI AAT'ATT Cuts at: 5 52 2595 3928 4140 5413 5 Size: 47 2543 1333 212 1273 2081 Fragments arranged by size: 2543 2081 1333 1273 212 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7489 StyI C'CwwG_G Cuts at: 513 1065 3468 3873 3897 4928 513 Size: 552 2403 405 24 1031 3074 Fragments arranged by size: 3074 2403 1031 552 405 24 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4261 5516 5533 5686 5871 6316 325 Size: 1255 2681 1255 17 153 185 445 1498 Fragments arranged by size: 2681 1498 1255 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3362 4170 4310 Size: 274 80 33 51 1443 1385 808 140 Cuts at: 4310 5735 7447 96 Size: 1425 1712 138 Fragments arranged by size: 1712 1443 1425 1385 808 274 140 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3147 3179 3486 3518 7449 7481 3147 Size: 32 307 32 3931 32 3155 Fragments arranged by size: 3931 3155 307 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7489 XbaI T'CTAG_A Cuts at: 4825 4825 Size: 7489 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3279 3279 Size: 7489 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7489 XmaI C'CCGG_G Cuts at: 1870 2037 2105 4814 1870 Size: 167 68 2709 4545 Fragments arranged by size: 4545 2709 167 68 XmnI GAAnn'nnTTC Cuts at: 3706 5618 3706 Size: 1912 5577 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5297 276 Size: 53 83 186 936 3763 2468 Fragments arranged by size: 3763 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstZ17I EcoICRI EcoNI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspOMI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI