Restriction map for
pFN21AA0970
(Circular) (Six-base) MAPSORT of: pFN21AA0970.seq Check: 4602 from: 1 to: 8131
pFN21AA0970 8131 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5941 278
Size: 53 83 186 936 4405 2468
Fragments arranged by size:
4405 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3853 5474 1937
Size: 1916 1621 4594
Fragments arranged by size:
4594 1916 1621
Acc65I G'GTAC_C
Cuts at: 5452 5452
Size: 8131
AclI AA'CG_TT
Cuts at: 1563 2389 6258 6631 1563
Size: 826 3869 373 3063
Fragments arranged by size:
3869 3063 826 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2895 3348 3531 4005 4018 4784 5144 6194
Size: 1263 453 183 474 13 766 360 1050
Cuts at: 6194 7578 7846 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 1263 1050 766 474 453 360
268 183 13
AflII C'TTAA_G
Cuts at: 820 1017 4736 820
Size: 197 3719 4215
Fragments arranged by size:
4215 3719 197
AflIII A'CryG_T
Cuts at: 1203 4139 7030 8007 1203
Size: 2936 2891 977 1327
Fragments arranged by size:
2936 2891 1327 977
AgeI A'CCGG_T
Cuts at: 6962 6962
Size: 8131
AhdI GACnn_n'nnGTC
Cuts at: 6860 6860
Size: 8131
AleI CACnn'nnGTG
Cuts at: 1166 5022 5255 1166
Size: 3856 233 4042
Fragments arranged by size:
4042 3856 233
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5273 5373 1184
Size: 4089 100 3942
Fragments arranged by size:
4089 3942 100
AlwNI CAG_nnn'CTG
Cuts at: 2918 4103 4330 4394 5874 7446 2918
Size: 1185 227 64 1480 1572 3603
Fragments arranged by size:
3603 1572 1480 1185 227 64
ApaLI G'TGCA_C
Cuts at: 4158 6188 7344 4158
Size: 2030 1156 4945
Fragments arranged by size:
4945 2030 1156
ApoI r'AATT_y
Cuts at: 1493 2989 3083 3155 3330 3446 3503 4617 4895
Size: 1496 94 72 175 116 57 1114 278
Cuts at: 4895 5108 5440 5666 5818 7927 1493
Size: 213 332 226 152 2109 1697
Fragments arranged by size:
2109 1697 1496 1114 332 278 226 213
175 152 116 94 72 57
AseI AT'TA_AT
Cuts at: 160 1032 2481 2715 6685 160
Size: 872 1449 234 3970 1606
Fragments arranged by size:
3970 1606 1449 872 234
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8131
AvaI C'yCGr_G
Cuts at: 1870 1943 5456 1870
Size: 73 3513 4545
Fragments arranged by size:
4545 3513 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2562 2595 2562
Size: 33 8098
BamHI G'GATC_C
Cuts at: 3592 5461 5828 3592
Size: 1869 367 5895
Fragments arranged by size:
5895 1869 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2134 3013 5452 6907 618
Size: 325 217 627 347 879 2439 1455 1842
Fragments arranged by size:
2439 1842 1455 879 627 347 325 217
BanII G_rGCy'C
Cuts at: 1391 2751 3107 5450 1391
Size: 1360 356 2343 4072
Fragments arranged by size:
4072 2343 1360 356
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2103 2295 4403 5072 928
Size: 971 204 192 2108 669 3987
Fragments arranged by size:
3987 2108 971 669 204 192
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3735 3769 5336 5370 6322 6356 1903
Size: 34 1798 34 1567 34 952 34 3678
Fragments arranged by size:
3678 1798 1567 952 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4840 6023 7233 4840
Size: 1183 1210 5738
Fragments arranged by size:
5738 1210 1183
BclI T'GATC_A
Cuts at: 1548 4495 5014 1548
Size: 2947 519 4665
Fragments arranged by size:
4665 2947 519
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6742 136
Size: 107 122 71 631 605 5070 1525
Fragments arranged by size:
5070 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3400 4145 8126 3400
Size: 745 3981 3405
Fragments arranged by size:
3981 3405 745
BlpI GC'TnA_GC
Cuts at: 5548 5548
Size: 8131
Bme1580I G_kGCm'C
Cuts at: 1790 4162 5338 6192 7348 1790
Size: 2372 1176 854 1156 2573
Fragments arranged by size:
2573 2372 1176 1156 854
BmgBI CAC'GTC
Cuts at: 1318 2237 1318
Size: 919 7212
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5862 6820 442
Size: 416 53 83 399 24 4445 958 1753
Fragments arranged by size:
4445 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8131
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2280 2286 3792 4238 6791 1239
Size: 1041 6 1506 446 2553 2579
Fragments arranged by size:
2579 2553 1506 1041 446 6
Bpu10I CC'TnA_GC
Cuts at: 2019 2019
Size: 8131
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2758 2831 2956 4116 6256 7121 7419 2758
Size: 73 125 1160 2140 865 298 3470
Fragments arranged by size:
3470 2140 1160 865 298 125 73
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3787 4274 6794 882
Size: 617 2288 487 2520 2219
Fragments arranged by size:
2520 2288 2219 617 487
BsaAI yAC'GTr
Cuts at: 493 1204 4043 5922 493
Size: 711 2839 1879 2702
Fragments arranged by size:
2839 2702 1879 711
BsaBI GATnn'nnATC
Cuts at: 8125 8125
Size: 8131
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5938 6320 6955 275
Size: 53 83 186 936 4405 382 635 1451
Fragments arranged by size:
4405 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5145 6564 6962 7236 7383 7794 5145
Size: 1419 398 274 147 411 5482
Fragments arranged by size:
5482 1419 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7906
Fragments arranged by size:
7906 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2178 4242 1186
Size: 992 2064 5075
Fragments arranged by size:
5075 2064 992
BseYI C'CCAG_C
Cuts at: 4479 4922 4975 7334 7830 4479
Size: 443 53 2359 496 4780
Fragments arranged by size:
4780 2359 496 443 53
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3821 4065 4365 1163
Size: 573 2085 244 300 4929
Fragments arranged by size:
4929 2085 573 300 244
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5581 6342 7370 1540
Size: 275 186 3580 761 1028 2301
Fragments arranged by size:
3580 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2751 3235 4162 5262 6192 6277 7348 2751
Size: 484 927 1100 930 85 1071 3534
Fragments arranged by size:
3534 1100 1071 930 927 484 85
BsmI GAATG_Cn'
Cuts at: 5642 5735 5642
Size: 93 8038
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2751 3107 3235 4162 5262 5338 5450
Size: 399 961 356 128 927 1100 76 112
Cuts at: 5450 6192 6277 7348 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1100 1071 961 927 742 399 356
128 112 85 76
BspEI T'CCGG_A
Cuts at: 7794 7794
Size: 8131
BspHI T'CATG_A
Cuts at: 6020 7750 6020
Size: 1730 6401
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5486 844
Size: 4642 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 6018 1394
Size: 429 4195 3507
Fragments arranged by size:
4195 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3011 3150 6626 6800 65
Size: 1183 1763 139 3476 174 1396
Fragments arranged by size:
3476 1763 1396 1183 174 139
BsrFI r'CCGG_y
Cuts at: 1700 1810 6775 6962 1700
Size: 110 4965 187 2869
Fragments arranged by size:
4965 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3995 96
Size: 3899 4232
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8131
BssSI C'ACGA_G
Cuts at: 6191 7203 6191
Size: 1012 7119
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4157 794
Size: 3363 4768
BstBI TT'CG_AA
Cuts at: 2110 6935 2110
Size: 4825 3306
BstEII G'GTnAC_C
Cuts at: 1184 2694 1184
Size: 1510 6621
BstYI r'GATC_y
Cuts at: 1972 3400 3592 4145 5461 5828 6213 6230 7682
Size: 1428 192 553 1316 367 385 17 1452
Cuts at: 7682 7768 7780 8126 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1428 1316 553 385 367 346
192 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2130 3223 3307 2130
Size: 1093 84 6954
Fragments arranged by size:
6954 1093 84
BtgI C'CryG_G
Cuts at: 513 1065 2856 513
Size: 552 1791 5788
Fragments arranged by size:
5788 1791 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4440 487
Size: 683 56 3214 4178
Fragments arranged by size:
4178 3214 683 56
BtsI GCAGTG_nn'
Cuts at: 5357 5647 6441 6461 5357
Size: 290 794 20 7027
Fragments arranged by size:
7027 794 290 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8131
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8096
DraI TTT'AAA
Cuts at: 2838 3121 3580 5435 2838
Size: 283 459 1855 5534
Fragments arranged by size:
5534 1855 459 283
DraIII CAC_nnn'GTG
Cuts at: 3020 5342 3020
Size: 2322 5809
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7138 809
Size: 507 5822 1802
Fragments arranged by size:
5822 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3168 5578 6467
Size: 54 1425 50 275 18 1338 2410 889
Cuts at: 6467 8096 8
Size: 1629 43
Fragments arranged by size:
2410 1629 1425 1338 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5578 1537
Size: 275 3766 4090
Fragments arranged by size:
4090 3766 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2107 5143 5267 6061 1353
Size: 754 3036 124 794 3423
Fragments arranged by size:
3423 3036 794 754 124
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3627 6718 7092 7238 387
Size: 1064 138 2038 3091 374 146 1280
Fragments arranged by size:
3091 2038 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2749 2749
Size: 8131
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2280 2286 2895 3348 3531 3792 4005
Size: 393 648 6 609 453 183 261 213
Cuts at: 4005 4018 4238 4784 5144 6194 6791 7578 7846
Size: 13 220 546 360 1050 597 787 268
Cuts at: 7846 1239
Size: 1524
Fragments arranged by size:
1524 1050 787 648 609 597 546 453
393 360 268 261 220 213 183 13
6
EcoO109I rG'GnC_Cy
Cuts at: 3887 4349 4931 5129 5230 5240 5325 3887
Size: 462 582 198 101 10 85 6693
Fragments arranged by size:
6693 582 462 198 101 85 10
EcoRI G'AATT_C
Cuts at: 4617 5108 5440 5818 4617
Size: 491 332 378 6930
Fragments arranged by size:
6930 491 378 332
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2648 2648
Size: 8131
FspI TGC'GCA
Cuts at: 6637 6637
Size: 8131
HaeII r_GCGC'y
Cuts at: 1678 7278 1678
Size: 5600 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2212 2244 2318 2350 2354
Size: 32 135 32 640 32 74 32 4
Cuts at: 2354 2386 2962 2994 3606 3638 3893 3925 4426
Size: 32 576 32 612 32 255 32 501
Cuts at: 4426 4458 4529 4561 4871 4903 6772 6804 6846
Size: 32 71 32 310 32 1869 32 42
Cuts at: 6846 6878 1373
Size: 32 2626
Fragments arranged by size:
2626 1869 640 612 576 501 310 255
135 74 71 42 32 32 32 32
32 32 32 32 32 32 32 32
32 4
HincII GTy'rAC
Cuts at: 669 1657 1938 4776 4884 5475 5721 669
Size: 988 281 2838 108 591 246 3079
Fragments arranged by size:
3079 2838 988 591 281 246 108
HindIII A'AGCT_T
Cuts at: 748 4908 748
Size: 4160 3971
HpaI GTT'AAC
Cuts at: 5721 5721
Size: 8131
KpnI G_GTAC'C
Cuts at: 5456 5456
Size: 8131
MfeI C'AATT_G
Cuts at: 5730 5730
Size: 8131
MluI A'CGCG_T
Cuts at: 8007 8007
Size: 8131
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2376 2661 2685 7245 7429 1305
Size: 1071 285 24 4560 184 2007
Fragments arranged by size:
4560 2007 1071 285 184 24
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6309
Fragments arranged by size:
6309 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3708 5022 5038 5255 5369 6089 6448
Size: 648 2542 1314 16 217 114 720 359
Cuts at: 6448 6607 518
Size: 159 2042
Fragments arranged by size:
2542 2042 1314 720 648 359 217 159
114 16
MspA1I CmG'CkG
Cuts at: 1606 3812 4979 5164 5547 6224 7372 7617 1606
Size: 2206 1167 185 383 677 1148 245 2120
Fragments arranged by size:
2206 2120 1167 1148 677 383 245 185
NcoI C'CATG_G
Cuts at: 513 1065 2856 513
Size: 552 1791 5788
Fragments arranged by size:
5788 1791 552
NdeI CA'TA_TG
Cuts at: 387 2241 2383 4659 387
Size: 1854 142 2276 3859
Fragments arranged by size:
3859 2276 1854 142
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8131
NotI GC'GGCC_GC
Cuts at: 5578 5578
Size: 8131
NspI r_CATG'y
Cuts at: 1132 1229 4143 4467 5489 7034 1132
Size: 97 2914 324 1022 1545 2229
Fragments arranged by size:
2914 2229 1545 1022 324 97
PasI CC'CwG_GG
Cuts at: 1348 4103 1348
Size: 2755 5376
PciI A'CATG_T
Cuts at: 4139 7030 4139
Size: 2891 5240
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 8131
PfoI T'CCnGG_A
Cuts at: 2071 4015 2071
Size: 1944 6187
PmeI GTTT'AAAC
Cuts at: 5435 5435
Size: 8131
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6200 6232 7713 7745 6200
Size: 32 1481 32 6586
Fragments arranged by size:
6586 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3887 4931 5129 5230 5240 5325 3887
Size: 1044 198 101 10 85 6693
Fragments arranged by size:
6693 1044 198 101 85 10
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8131
PsiI TTA'TAA
Cuts at: 2501 5701 2501
Size: 3200 4931
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2937 2969 2937
Size: 32 8099
PstI C_TGCA'G
Cuts at: 830 5483 830
Size: 4653 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8131
PvuII CAG'CTG
Cuts at: 3812 4979 5164 3812
Size: 1167 185 6779
Fragments arranged by size:
6779 1167 185
SacI G_AGCT'C
Cuts at: 2751 2751
Size: 8131
SalI G'TCGA_C
Cuts at: 1936 5473 1936
Size: 3537 4594
SanDI GG'GwC_CC
Cuts at: 3887 5129 3887
Size: 1242 6889
SapI GCTCTTCn'nnn_
Cuts at: 5267 5267
Size: 8131
SbfI CC_TGCA'GG
Cuts at: 5483 5483
Size: 8131
ScaI AGT'ACT
Cuts at: 2685 2779 3436 5417 6379 8091 2685
Size: 94 657 1981 962 1712 2725
Fragments arranged by size:
2725 1981 1712 962 657 94
SexAI A'CCwGG_T
Cuts at: 2194 2690 2194
Size: 496 7635
SfcI C'TryA_G
Cuts at: 826 1045 3945 4095 4519 5245 5479 6614 7295
Size: 219 2900 150 424 726 234 1135 681
Cuts at: 7295 7486 826
Size: 191 1471
Fragments arranged by size:
2900 1471 1135 726 681 424 234 219
191 150
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8131
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8131
SmaI CCC'GGG
Cuts at: 1872 5458 1872
Size: 3586 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2773 2846 2935 4131 4736 6235
Size: 197 926 830 73 89 1196 605 1499
Cuts at: 6235 7136 7398 820
Size: 901 262 1553
Fragments arranged by size:
1553 1499 1196 926 901 830 605 262
197 89 73
SnaBI TAC'GTA
Cuts at: 493 5922 493
Size: 5429 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8131
SphI G_CATG'C
Cuts at: 1132 5489 1132
Size: 4357 3774
SspI AAT'ATT
Cuts at: 5 52 6055 5
Size: 47 6003 2081
Fragments arranged by size:
6003 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8131
StyI C'CwwG_G
Cuts at: 513 1065 2416 2856 2966 3264 3976 4636 5570
Size: 552 1351 440 110 298 712 660 934
Cuts at: 5570 513
Size: 3074
Fragments arranged by size:
3074 1351 934 712 660 552 440 298
110
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3928 4331 6158 6175 6328 6513 6958
Size: 1255 2348 403 1827 17 153 185 445
Cuts at: 6958 325
Size: 1498
Fragments arranged by size:
2348 1827 1498 1255 445 403 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2244 2683 2777
Size: 274 80 33 51 1443 267 439 94
Cuts at: 2777 3434 3995 4864 5415 6377 8089 96
Size: 657 561 869 551 962 1712 138
Fragments arranged by size:
1712 1443 962 869 657 561 551 439
274 267 138 94 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 8091 8123 8091
Size: 32 8099
Tth111I GACn'n_nGTC
Cuts at: 1316 4771 4848 1316
Size: 3455 77 4599
Fragments arranged by size:
4599 3455 77
XbaI T'CTAG_A
Cuts at: 5467 5467
Size: 8131
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8131
XmaI C'CCGG_G
Cuts at: 1870 5456 1870
Size: 3586 4545
XmnI GAAnn'nnTTC
Cuts at: 2628 2908 5101 6260 2628
Size: 280 2193 1159 4499
Fragments arranged by size:
4499 2193 1159 280
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5939 276
Size: 53 83 186 936 4405 2468
Fragments arranged by size:
4405 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PsrI PstI PvuI PvuII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspOMI PspXI RsrII SacII SfiI SfoI
SrfI SwaI XcmI