(Circular) (Six-base) MAPSORT of: pFN21AA0909.seq Check: 1185 from: 1 to: 8311 pFN21AA0909 8311 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3296 3430 3787 3973 3296 Size: 134 357 186 7634 Fragments arranged by size: 7634 357 186 134 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6121 278 Size: 53 83 186 936 4585 2468 Fragments arranged by size: 4585 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5654 1937 Size: 3717 4594 Acc65I G'GTAC_C Cuts at: 5632 5632 Size: 8311 AclI AA'CG_TT Cuts at: 1563 6438 6811 1563 Size: 4875 373 3063 Fragments arranged by size: 4875 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2072 2345 2546 2579 3490 4737 5031 5225 Size: 440 273 201 33 911 1247 294 194 Cuts at: 5225 5582 6374 7758 8026 1632 Size: 357 792 1384 268 1917 Fragments arranged by size: 1917 1384 1247 911 792 440 357 294 273 268 201 194 33 AfeI AGC'GCT Cuts at: 5243 5243 Size: 8311 AflII C'TTAA_G Cuts at: 820 1017 5282 820 Size: 197 4265 3849 Fragments arranged by size: 4265 3849 197 AflIII A'CryG_T Cuts at: 1203 7210 8187 1203 Size: 6007 977 1327 Fragments arranged by size: 6007 1327 977 AgeI A'CCGG_T Cuts at: 7142 7142 Size: 8311 AhdI GACnn_n'nnGTC Cuts at: 3892 7040 3892 Size: 3148 5163 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8311 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4173 1184 Size: 2989 5322 AlwNI CAG_nnn'CTG Cuts at: 3977 4351 4505 4619 4928 5231 5578 6054 7626 Size: 374 154 114 309 303 347 476 1572 Cuts at: 7626 3977 Size: 4662 Fragments arranged by size: 4662 1572 476 374 347 309 303 154 114 ApaI G_GGCC'C Cuts at: 3819 4722 3819 Size: 903 7408 ApaLI G'TGCA_C Cuts at: 2435 6368 7524 2435 Size: 3933 1156 3222 Fragments arranged by size: 3933 3222 1156 ApoI r'AATT_y Cuts at: 1493 5620 5846 5998 8107 1493 Size: 4127 226 152 2109 1697 Fragments arranged by size: 4127 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6865 160 Size: 872 5833 1606 Fragments arranged by size: 5833 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8311 AvaI C'yCGr_G Cuts at: 1870 1943 2304 2535 2772 2946 3206 3279 4401 Size: 73 361 231 237 174 260 73 1122 Cuts at: 4401 4892 5160 5636 1870 Size: 491 268 476 4545 Fragments arranged by size: 4545 1122 491 476 361 268 260 237 231 174 73 73 AvrII C'CTAG_G Cuts at: 3053 3053 Size: 8311 BamHI G'GATC_C Cuts at: 5641 6008 5641 Size: 367 7944 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3985 4547 5632 7087 618 Size: 325 217 627 2198 562 1085 1455 1842 Fragments arranged by size: 2198 1842 1455 1085 627 562 325 217 BanII G_rGCy'C Cuts at: 1391 2772 2853 2916 3267 3363 3504 3819 3853 Size: 1381 81 63 351 96 141 315 34 Cuts at: 3853 4467 4722 4938 5630 1391 Size: 614 255 216 692 4072 Fragments arranged by size: 4072 1381 692 614 351 315 255 216 141 96 81 63 34 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2078 2303 928 Size: 971 179 225 6936 Fragments arranged by size: 6936 971 225 179 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6502 6536 1903 Size: 34 4565 34 3678 Fragments arranged by size: 4565 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6203 7413 6203 Size: 1210 7101 BclI T'GATC_A Cuts at: 1548 2157 3709 1548 Size: 609 1552 6150 Fragments arranged by size: 6150 1552 609 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3028 6922 136 Size: 107 122 71 631 605 1356 3894 1525 Fragments arranged by size: 3894 1525 1356 631 605 122 107 71 BglII A'GATC_T Cuts at: 2055 8306 2055 Size: 6251 2060 BlpI GC'TnA_GC Cuts at: 5728 5728 Size: 8311 Bme1580I G_kGCm'C Cuts at: 1790 2197 2439 3819 4300 4582 4722 6372 7528 Size: 407 242 1380 481 282 140 1650 1156 Cuts at: 7528 1790 Size: 2573 Fragments arranged by size: 2573 1650 1380 1156 481 407 282 242 140 BmgBI CAC'GTC Cuts at: 1318 4563 1318 Size: 3245 5066 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3111 6042 7000 Size: 416 53 83 399 24 1694 2931 958 Cuts at: 7000 442 Size: 1753 Fragments arranged by size: 2931 1753 1694 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8311 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3712 5089 3712 Size: 1377 6934 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2094 2099 2396 2480 3017 3030 3281 3518 Size: 855 5 297 84 537 13 251 237 Cuts at: 3518 3959 3996 4477 4755 4920 4991 6971 1239 Size: 441 37 481 278 165 71 1980 2579 Fragments arranged by size: 2579 1980 855 537 481 441 297 278 251 237 165 84 71 37 13 5 Bpu10I CC'TnA_GC Cuts at: 4193 4193 Size: 8311 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3347 4451 6436 7301 7599 3347 Size: 1104 1985 865 298 4059 Fragments arranged by size: 4059 1985 1104 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4181 4937 6974 882 Size: 617 2682 756 2037 2219 Fragments arranged by size: 2682 2219 2037 756 617 BsaAI yAC'GTr Cuts at: 493 1204 6102 493 Size: 711 4898 2702 Fragments arranged by size: 4898 2702 711 BsaBI GATnn'nnATC Cuts at: 8305 8305 Size: 8311 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3020 6118 6500 7135 Size: 53 83 186 936 1487 3098 382 635 Cuts at: 7135 275 Size: 1451 Fragments arranged by size: 3098 1487 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3915 4855 5250 5442 6744 7142 7416 7563 7974 Size: 940 395 192 1302 398 274 147 411 Cuts at: 7974 3915 Size: 4252 Fragments arranged by size: 4252 1302 940 411 398 395 274 192 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2531 2561 3465 3495 5396 Size: 30 165 30 1128 30 904 30 1901 Cuts at: 5396 5426 5462 5492 1178 Size: 30 36 30 3997 Fragments arranged by size: 3997 1901 1128 904 165 36 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2153 2555 2811 2884 2972 2979 3102 3371 Size: 967 402 256 73 88 7 123 269 Cuts at: 3371 3471 3999 4480 4513 4676 4717 1186 Size: 100 528 481 33 163 41 4780 Fragments arranged by size: 4780 967 528 481 402 269 256 163 123 100 88 73 41 33 7 BseYI C'CCAG_C Cuts at: 2452 3582 3977 4991 5006 5592 7514 8010 2452 Size: 1130 395 1014 15 586 1922 496 2753 Fragments arranged by size: 2753 1922 1130 1014 586 496 395 15 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2651 4133 4587 1163 Size: 573 915 1482 454 4887 Fragments arranged by size: 4887 1482 915 573 454 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2512 5761 6522 7550 1540 Size: 275 186 511 3249 761 1028 2301 Fragments arranged by size: 3249 2301 1028 761 511 275 186 BsiHKAI G_wGCw'C Cuts at: 2439 2853 3363 3660 3837 4326 5394 6372 6457 Size: 414 510 297 177 489 1068 978 85 Cuts at: 6457 7528 2439 Size: 1071 3222 Fragments arranged by size: 3222 1071 1068 978 510 489 414 297 177 85 BsmI GAATG_Cn' Cuts at: 4356 5822 5915 4356 Size: 1466 93 6752 Fragments arranged by size: 6752 1466 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4525 4525 Size: 8311 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2197 2439 2772 2853 2916 3267 3363 Size: 399 407 242 333 81 63 351 96 Cuts at: 3363 3504 3660 3819 3837 3853 4300 4326 4467 Size: 141 156 159 18 16 447 26 141 Cuts at: 4467 4582 4722 4938 5394 5630 6372 6457 7528 Size: 115 140 216 456 236 742 85 1071 Cuts at: 7528 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 456 447 407 399 351 333 242 236 216 159 156 141 141 140 115 96 85 81 63 26 18 16 BspEI T'CCGG_A Cuts at: 3915 7974 3915 Size: 4059 4252 BspHI T'CATG_A Cuts at: 5520 6200 7930 5520 Size: 680 1730 5901 Fragments arranged by size: 5901 1730 680 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3296 3430 3787 3973 5666 844 Size: 2452 134 357 186 1693 3489 Fragments arranged by size: 3489 2452 1693 357 186 134 BsrBI CCG'CTC Cuts at: 1394 1823 3221 4252 4861 4918 5384 5412 6198 Size: 429 1398 1031 609 57 466 28 786 Cuts at: 6198 1394 Size: 3507 Fragments arranged by size: 3507 1398 1031 786 609 466 429 57 28 BsrDI GCAATG_nn' Cuts at: 65 1248 6806 6980 65 Size: 1183 5558 174 1396 Fragments arranged by size: 5558 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5196 6955 7142 1700 Size: 110 3386 1759 187 2869 Fragments arranged by size: 3386 2869 1759 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8311 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8311 BssSI C'ACGA_G Cuts at: 5132 6371 7383 5132 Size: 1239 1012 6060 Fragments arranged by size: 6060 1239 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8311 BstBI TT'CG_AA Cuts at: 4044 7115 4044 Size: 3071 5240 BstEII G'GTnAC_C Cuts at: 1184 2267 1184 Size: 1083 7228 BstXI CCAn_nnnn'nTGG Cuts at: 3034 3070 3173 3496 4037 4969 3034 Size: 36 103 323 541 932 6376 Fragments arranged by size: 6376 932 541 323 103 36 BstYI r'GATC_y Cuts at: 1972 2055 5641 6008 6393 6410 7862 7948 7960 Size: 83 3586 367 385 17 1452 86 12 Cuts at: 7960 8306 1972 Size: 346 1977 Fragments arranged by size: 3586 1977 1452 385 367 346 86 83 17 12 BtgI C'CryG_G Cuts at: 513 1065 4117 513 Size: 552 3052 4707 Fragments arranged by size: 4707 3052 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3700 487 Size: 683 56 2474 5098 Fragments arranged by size: 5098 2474 683 56 BtsI GCAGTG_nn' Cuts at: 2698 2970 3081 3726 5827 6621 6641 2698 Size: 272 111 645 2101 794 20 4368 Fragments arranged by size: 4368 2101 794 645 272 111 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8311 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2011 2046 529 Size: 35 1447 35 6794 Fragments arranged by size: 6794 1447 35 35 DraI TTT'AAA Cuts at: 5615 5615 Size: 8311 DraIII CAC_nnn'GTG Cuts at: 2629 2629 Size: 8311 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7318 809 Size: 507 6002 1802 Fragments arranged by size: 6002 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4959 5316 5451 Size: 54 1425 50 275 18 3129 357 135 Cuts at: 5451 5604 5758 6647 8276 8 Size: 153 154 889 1629 43 Fragments arranged by size: 3129 1629 1425 889 357 275 154 153 135 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5758 1537 Size: 275 3946 4090 Fragments arranged by size: 4090 3946 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2374 2601 2890 3310 3448 6241 1353 Size: 1021 227 289 420 138 2793 3423 Fragments arranged by size: 3423 2793 1021 420 289 227 138 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4219 6898 7272 7418 387 Size: 1064 138 2630 2679 374 146 1280 Fragments arranged by size: 2679 2630 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2851 3361 2851 Size: 510 7801 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2072 2094 2099 2345 2396 2480 2546 Size: 393 440 22 5 246 51 84 66 Cuts at: 2546 2579 3017 3030 3281 3490 3518 3959 3996 Size: 33 438 13 251 209 28 441 37 Cuts at: 3996 4477 4737 4755 4920 4991 5031 5225 5582 Size: 481 260 18 165 71 40 194 357 Cuts at: 5582 6374 6971 7758 8026 1239 Size: 792 597 787 268 1524 Fragments arranged by size: 1524 792 787 597 481 441 440 438 393 357 268 260 251 246 209 194 165 84 71 66 51 40 37 33 28 22 18 13 5 EcoO109I rG'GnC_Cy Cuts at: 3284 3476 3668 3815 3816 3998 4034 4100 4718 Size: 192 192 147 1 182 36 66 618 Cuts at: 4718 4719 3284 Size: 1 6876 Fragments arranged by size: 6876 618 192 192 182 147 66 36 1 1 EcoRI G'AATT_C Cuts at: 5620 5998 5620 Size: 378 7933 FspI TGC'GCA Cuts at: 6817 6817 Size: 8311 HaeII r_GCGC'y Cuts at: 1678 2398 5245 5539 7458 1678 Size: 720 2847 294 1919 2531 Fragments arranged by size: 2847 2531 1919 720 294 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2132 2164 2171 2194 2203 Size: 32 135 32 560 32 7 23 9 Cuts at: 2203 2226 3528 3560 3878 3910 4253 4285 4808 Size: 23 1302 32 318 32 343 32 523 Cuts at: 4808 4840 4934 4966 6952 6984 7026 7058 1373 Size: 32 94 32 1986 32 42 32 2626 Fragments arranged by size: 2626 1986 1302 560 523 343 318 135 94 42 32 32 32 32 32 32 32 32 32 32 23 23 9 7 HincII GTy'rAC Cuts at: 669 1657 1938 4246 5655 5901 669 Size: 988 281 2308 1409 246 3079 Fragments arranged by size: 3079 2308 1409 988 281 246 HindIII A'AGCT_T Cuts at: 748 4222 748 Size: 3474 4837 HpaI GTT'AAC Cuts at: 5901 5901 Size: 8311 KpnI G_GTAC'C Cuts at: 5636 5636 Size: 8311 MfeI C'AATT_G Cuts at: 5910 5910 Size: 8311 MluI A'CGCG_T Cuts at: 8187 8187 Size: 8311 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3599 4316 4521 7425 7609 1305 Size: 2294 717 205 2904 184 2007 Fragments arranged by size: 2904 2294 2007 717 205 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4961 5606 10 Size: 54 1425 343 3129 645 2715 Fragments arranged by size: 3129 2715 1425 645 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3719 6269 6628 6787 518 Size: 648 2553 2550 359 159 2042 Fragments arranged by size: 2553 2550 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2101 2557 3004 3275 3586 3977 4425 5140 Size: 495 456 447 271 311 391 448 715 Cuts at: 5140 5231 5266 5727 6404 7552 7797 1606 Size: 91 35 461 677 1148 245 2120 Fragments arranged by size: 2120 1148 715 677 495 461 456 448 447 391 311 271 245 91 35 NaeI GCC'GGC Cuts at: 5198 5198 Size: 8311 NcoI C'CATG_G Cuts at: 513 1065 4117 513 Size: 552 3052 4707 Fragments arranged by size: 4707 3052 552 NdeI CA'TA_TG Cuts at: 387 3172 387 Size: 2785 5526 NgoMIV G'CCGG_C Cuts at: 5196 5196 Size: 8311 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8311 NotI GC'GGCC_GC Cuts at: 5758 5758 Size: 8311 NspI r_CATG'y Cuts at: 1132 1229 5669 7214 1132 Size: 97 4440 1545 2229 Fragments arranged by size: 4440 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 4165 4300 1348 Size: 2817 135 5359 Fragments arranged by size: 5359 2817 135 PciI A'CATG_T Cuts at: 7210 7210 Size: 8311 PflMI CCAn_nnn'nTGG Cuts at: 1108 2338 3644 4505 4953 5578 1108 Size: 1230 1306 861 448 625 3841 Fragments arranged by size: 3841 1306 1230 861 625 448 PmeI GTTT'AAAC Cuts at: 5615 5615 Size: 8311 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6380 6412 7893 7925 6380 Size: 32 1481 32 6766 Fragments arranged by size: 6766 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3284 3476 3668 3998 4100 3284 Size: 192 192 330 102 7495 Fragments arranged by size: 7495 330 192 192 102 PshAI GACnn'nnGTC Cuts at: 1613 4506 1613 Size: 2893 5418 PsiI TTA'TAA Cuts at: 5881 5881 Size: 8311 PspOMI G'GGCC_C Cuts at: 3815 4718 3815 Size: 903 7408 PstI C_TGCA'G Cuts at: 830 2483 2693 5663 830 Size: 1653 210 2970 3478 Fragments arranged by size: 3478 2970 1653 210 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8311 PvuII CAG'CTG Cuts at: 2557 3275 3586 3977 5231 5266 2557 Size: 718 311 391 1254 35 5602 Fragments arranged by size: 5602 1254 718 391 311 35 SacI G_AGCT'C Cuts at: 2853 3363 2853 Size: 510 7801 SalI G'TCGA_C Cuts at: 1936 5653 1936 Size: 3717 4594 SanDI GG'GwC_CC Cuts at: 3284 3284 Size: 8311 SbfI CC_TGCA'GG Cuts at: 5663 5663 Size: 8311 ScaI AGT'ACT Cuts at: 3877 6559 8271 3877 Size: 2682 1712 3917 Fragments arranged by size: 3917 2682 1712 SexAI A'CCwGG_T Cuts at: 2625 2625 Size: 8311 SfcI C'TryA_G Cuts at: 826 1045 2479 2616 2689 4439 5659 6794 7475 Size: 219 1434 137 73 1750 1220 1135 681 Cuts at: 7475 7666 826 Size: 191 1471 Fragments arranged by size: 1750 1471 1434 1220 1135 681 219 191 137 73 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8311 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8311 SmaI CCC'GGG Cuts at: 1872 2537 4403 5638 1872 Size: 665 1866 1235 4545 Fragments arranged by size: 4545 1866 1235 665 SmlI C'TyrA_G Cuts at: 820 1017 1943 2946 3362 4430 5282 6415 7316 Size: 197 926 1003 416 1068 852 1133 901 Cuts at: 7316 7578 820 Size: 262 1553 Fragments arranged by size: 1553 1133 1068 1003 926 901 852 416 262 197 SnaBI TAC'GTA Cuts at: 493 6102 493 Size: 5609 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8311 SphI G_CATG'C Cuts at: 1132 5669 1132 Size: 4537 3774 SspI AAT'ATT Cuts at: 5 52 6235 5 Size: 47 6183 2081 Fragments arranged by size: 6183 2081 47 StuI AGG'CCT Cuts at: 1517 2209 3058 3464 1517 Size: 692 849 406 6364 Fragments arranged by size: 6364 849 692 406 StyI C'CwwG_G Cuts at: 513 1065 2013 2856 3053 3429 3671 4117 4713 Size: 552 948 843 197 376 242 446 596 Cuts at: 4713 5750 513 Size: 1037 3074 Fragments arranged by size: 3074 1037 948 843 596 552 446 376 242 197 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2663 2667 3400 3692 6338 6355 6508 Size: 1255 1083 4 733 292 2646 17 153 Cuts at: 6508 6693 7138 325 Size: 185 445 1498 Fragments arranged by size: 2646 1498 1255 1083 733 445 292 185 153 17 4 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3875 5187 5256 Size: 274 80 33 51 1443 1898 1312 69 Cuts at: 5256 6557 8269 96 Size: 1301 1712 138 Fragments arranged by size: 1898 1712 1443 1312 1301 274 138 80 69 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5396 5428 8271 8303 5396 Size: 32 2843 32 5404 Fragments arranged by size: 5404 2843 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2426 4948 1316 Size: 1110 2522 4679 Fragments arranged by size: 4679 2522 1110 XbaI T'CTAG_A Cuts at: 5647 5647 Size: 8311 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2074 3031 4089 4658 4954 5092 2074 Size: 957 1058 569 296 138 5293 Fragments arranged by size: 5293 1058 957 569 296 138 XhoI C'TCGA_G Cuts at: 1943 2946 1943 Size: 1003 7308 XmaI C'CCGG_G Cuts at: 1870 2535 4401 5636 1870 Size: 665 1866 1235 4545 Fragments arranged by size: 4545 1866 1235 665 XmnI GAAnn'nnTTC Cuts at: 5586 6440 5586 Size: 854 7457 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6119 276 Size: 53 83 186 936 4585 2468 Fragments arranged by size: 4585 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI BaeI BbeI BbvCI BfrBI BsiWI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII SapI SfiI SfoI SrfI SwaI