(Circular) (Six-base) MAPSORT of: pFN21AA0905.seq Check: 728 from: 1 to: 8365 pFN21AA0905 8365 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2267 3806 2267 Size: 1539 6826 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6175 278 Size: 53 83 186 936 4639 2468 Fragments arranged by size: 4639 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5708 1937 Size: 3771 4594 Acc65I G'GTAC_C Cuts at: 2208 4606 5686 2208 Size: 2398 1080 4887 Fragments arranged by size: 4887 2398 1080 AclI AA'CG_TT Cuts at: 1563 5449 6492 6865 1563 Size: 3886 1043 373 3063 Fragments arranged by size: 3886 3063 1043 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3173 3323 3630 4139 4881 5109 5385 5581 Size: 1541 150 307 509 742 228 276 196 Cuts at: 5581 6428 7812 8080 1632 Size: 847 1384 268 1917 Fragments arranged by size: 1917 1541 1384 847 742 509 307 276 268 228 196 150 AfeI AGC'GCT Cuts at: 5390 5390 Size: 8365 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8168 AflIII A'CryG_T Cuts at: 1203 7264 8241 1203 Size: 6061 977 1327 Fragments arranged by size: 6061 1327 977 AgeI A'CCGG_T Cuts at: 2712 7196 2712 Size: 4484 3881 AhdI GACnn_n'nnGTC Cuts at: 5098 7094 5098 Size: 1996 6369 AleI CACnn'nnGTG Cuts at: 1166 2917 3896 1166 Size: 1751 979 5635 Fragments arranged by size: 5635 1751 979 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2121 4090 4716 1184 Size: 937 1969 626 4833 Fragments arranged by size: 4833 1969 937 626 AlwNI CAG_nnn'CTG Cuts at: 2822 4162 4457 6108 7680 2822 Size: 1340 295 1651 1572 3507 Fragments arranged by size: 3507 1651 1572 1340 295 ApaLI G'TGCA_C Cuts at: 6422 7578 6422 Size: 1156 7209 ApoI r'AATT_y Cuts at: 1493 3111 3407 3659 3995 4407 5674 5900 6052 Size: 1618 296 252 336 412 1267 226 152 Cuts at: 6052 8161 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1618 1267 412 336 296 252 226 152 AseI AT'TA_AT Cuts at: 160 1032 6919 160 Size: 872 5887 1606 Fragments arranged by size: 5887 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8365 AvaI C'yCGr_G Cuts at: 1870 1943 5690 1870 Size: 73 3747 4545 Fragments arranged by size: 4545 3747 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4590 4623 4590 Size: 33 8332 BamHI G'GATC_C Cuts at: 5695 6062 5695 Size: 367 7998 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2208 4606 5261 5686 7141 Size: 325 217 627 421 2398 655 425 1455 Cuts at: 7141 618 Size: 1842 Fragments arranged by size: 2398 1842 1455 655 627 425 421 325 217 BanII G_rGCy'C Cuts at: 1391 2051 3602 4885 5275 5684 1391 Size: 660 1551 1283 390 409 4072 Fragments arranged by size: 4072 1551 1283 660 409 390 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 5145 928 Size: 971 3246 4148 Fragments arranged by size: 4148 3246 971 BbvCI CC'TCA_GC Cuts at: 3320 4689 3320 Size: 1369 6996 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6556 6590 1903 Size: 34 4619 34 3678 Fragments arranged by size: 4619 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3479 4662 6257 7467 3479 Size: 1183 1595 1210 4377 Fragments arranged by size: 4377 1595 1210 1183 BclI T'GATC_A Cuts at: 1548 1548 Size: 8365 BfrBI ATG'CAT Cuts at: 2647 4540 5569 2647 Size: 1893 1029 5443 Fragments arranged by size: 5443 1893 1029 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2039 3551 5270 Size: 107 122 71 631 605 367 1512 1719 Cuts at: 5270 6976 136 Size: 1706 1525 Fragments arranged by size: 1719 1706 1525 1512 631 605 367 122 107 71 BglII A'GATC_T Cuts at: 3499 8360 3499 Size: 4861 3504 BlpI GC'TnA_GC Cuts at: 2094 3129 3258 5782 2094 Size: 1035 129 2524 4677 Fragments arranged by size: 4677 2524 1035 129 Bme1580I G_kGCm'C Cuts at: 1790 5266 6426 7582 1790 Size: 3476 1160 1156 2573 Fragments arranged by size: 3476 2573 1160 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8365 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2369 2565 4773 Size: 416 53 83 399 24 952 196 2208 Cuts at: 4773 5092 6096 7054 442 Size: 319 1004 958 1753 Fragments arranged by size: 2208 1753 1004 958 952 416 399 319 196 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8365 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4067 4067 Size: 8365 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2283 2349 3696 4569 4798 4872 4983 5115 Size: 1044 66 1347 873 229 74 111 132 Cuts at: 5115 7025 1239 Size: 1910 2579 Fragments arranged by size: 2579 1910 1347 1044 873 229 132 111 74 66 Bpu10I CC'TnA_GC Cuts at: 3320 4281 4689 3320 Size: 961 408 6996 Fragments arranged by size: 6996 961 408 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2153 4047 4113 4191 6490 7355 7653 2153 Size: 1894 66 78 2299 865 298 2865 Fragments arranged by size: 2865 2299 1894 865 298 78 66 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5597 7028 882 Size: 617 4098 1431 2219 Fragments arranged by size: 4098 2219 1431 617 BsaAI yAC'GTr Cuts at: 493 1204 6156 493 Size: 711 4952 2702 Fragments arranged by size: 4952 2702 711 BsaBI GATnn'nnATC Cuts at: 8359 8359 Size: 8365 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6172 6554 7189 275 Size: 53 83 186 936 4639 382 635 1451 Fragments arranged by size: 4639 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2712 6798 7196 7470 7617 8028 2712 Size: 4086 398 274 147 411 3049 Fragments arranged by size: 4086 3049 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3534 3564 4368 4398 4413 Size: 30 165 30 2131 30 804 30 15 Cuts at: 4413 4443 5447 5477 1178 Size: 30 1004 30 4066 Fragments arranged by size: 4066 2131 1004 804 165 30 30 30 30 30 30 15 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2185 3608 4695 4827 1186 Size: 999 1423 1087 132 4724 Fragments arranged by size: 4724 1423 1087 999 132 BseYI C'CCAG_C Cuts at: 4644 5656 7568 8064 4644 Size: 1012 1912 496 4945 Fragments arranged by size: 4945 1912 1012 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3371 4107 5321 1163 Size: 573 1635 736 1214 4207 Fragments arranged by size: 4207 1635 1214 736 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5815 6576 7604 1540 Size: 275 186 3814 761 1028 2301 Fragments arranged by size: 3814 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3633 4372 4885 5352 6426 6511 7582 3633 Size: 739 513 467 1074 85 1071 4416 Fragments arranged by size: 4416 1074 1071 739 513 467 85 BsiWI C'GTAC_G Cuts at: 4413 4413 Size: 8365 BsmI GAATG_Cn' Cuts at: 2128 2649 3243 5876 5969 2128 Size: 521 594 2633 93 4524 Fragments arranged by size: 4524 2633 594 521 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2051 3602 3633 4372 4885 5266 5275 Size: 399 261 1551 31 739 513 381 9 Cuts at: 5275 5352 5684 6426 6511 7582 1391 Size: 77 332 742 85 1071 2174 Fragments arranged by size: 2174 1551 1071 742 739 513 399 381 332 261 85 77 31 9 BspEI T'CCGG_A Cuts at: 8028 8028 Size: 8365 BspHI T'CATG_A Cuts at: 5076 6254 7984 5076 Size: 1178 1730 5457 Fragments arranged by size: 5457 1730 1178 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2267 3806 5720 844 Size: 1423 1539 1914 3489 Fragments arranged by size: 3489 1914 1539 1423 BsrBI CCG'CTC Cuts at: 1394 1823 6252 1394 Size: 429 4429 3507 Fragments arranged by size: 4429 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2523 4683 5520 6860 7034 65 Size: 1183 1275 2160 837 1340 174 1396 Fragments arranged by size: 2160 1396 1340 1275 1183 837 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2712 7009 7196 1700 Size: 110 902 4297 187 2869 Fragments arranged by size: 4297 2869 902 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8365 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8365 BssSI C'ACGA_G Cuts at: 3459 6425 7437 3459 Size: 2966 1012 4387 Fragments arranged by size: 4387 2966 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3349 5308 794 Size: 2555 1959 3851 Fragments arranged by size: 3851 2555 1959 BstBI TT'CG_AA Cuts at: 3175 7169 3175 Size: 3994 4371 BstEII G'GTnAC_C Cuts at: 1184 2715 5090 1184 Size: 1531 2375 4459 Fragments arranged by size: 4459 2375 1531 BstXI CCAn_nnnn'nTGG Cuts at: 3805 4273 5656 3805 Size: 468 1383 6514 Fragments arranged by size: 6514 1383 468 BstYI r'GATC_y Cuts at: 1972 2455 2593 2737 2818 3499 4159 5377 5695 Size: 483 138 144 81 681 660 1218 318 Cuts at: 5695 6062 6447 6464 7916 8002 8014 8360 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1218 681 660 483 385 367 346 318 144 138 86 81 17 12 BtgI C'CryG_G Cuts at: 513 1065 4212 4504 5194 513 Size: 552 3147 292 690 3684 Fragments arranged by size: 3684 3147 690 552 292 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7626 Fragments arranged by size: 7626 683 56 BtsI GCAGTG_nn' Cuts at: 3891 3903 4907 4931 5881 6675 6695 3891 Size: 12 1004 24 950 794 20 5561 Fragments arranged by size: 5561 1004 950 794 24 20 12 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8365 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8330 DraI TTT'AAA Cuts at: 5669 5669 Size: 8365 DraIII CAC_nnn'GTG Cuts at: 2765 2916 3283 3584 2765 Size: 151 367 301 7546 Fragments arranged by size: 7546 367 301 151 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7372 809 Size: 507 6056 1802 Fragments arranged by size: 6056 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2575 3789 3807 Size: 54 1425 50 275 18 745 1214 18 Cuts at: 3807 4459 5182 5812 6701 8330 8 Size: 652 723 630 889 1629 43 Fragments arranged by size: 1629 1425 1214 889 745 723 652 630 275 54 50 43 18 18 EagI C'GGCC_G Cuts at: 1537 1812 5812 1537 Size: 275 4000 4090 Fragments arranged by size: 4090 4000 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2203 3606 4807 6295 1353 Size: 850 1403 1201 1488 3423 Fragments arranged by size: 3423 1488 1403 1201 850 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4887 6952 7326 7472 387 Size: 1064 138 3298 2065 374 146 1280 Fragments arranged by size: 3298 2065 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4883 4883 Size: 8365 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2283 2349 3173 3323 3630 3696 4139 Size: 393 651 66 824 150 307 66 443 Cuts at: 4139 4569 4798 4872 4881 4983 5109 5115 5385 Size: 430 229 74 9 102 126 6 270 Cuts at: 5385 5581 6428 7025 7812 8080 1239 Size: 196 847 597 787 268 1524 Fragments arranged by size: 1524 847 824 787 651 597 443 430 393 307 270 268 229 196 150 126 102 74 66 66 9 6 EcoNI CCTnn'n_nnAGG Cuts at: 4386 4386 Size: 8365 EcoO109I rG'GnC_Cy Cuts at: 2228 4973 2228 Size: 2745 5620 EcoRI G'AATT_C Cuts at: 5674 6052 5674 Size: 378 7987 EcoRV GAT'ATC Cuts at: 2515 2515 Size: 8365 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3460 4161 5169 3460 Size: 701 1008 6656 Fragments arranged by size: 6656 1008 701 FspI TGC'GCA Cuts at: 6871 6871 Size: 8365 HaeII r_GCGC'y Cuts at: 1678 5392 7512 1678 Size: 3714 2120 2531 Fragments arranged by size: 3714 2531 2120 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2162 2194 2722 2754 3813 Size: 32 135 32 590 32 528 32 1059 Cuts at: 3813 3845 4111 4143 5084 5116 7006 7038 7080 Size: 32 266 32 941 32 1890 32 42 Cuts at: 7080 7112 1373 Size: 32 2626 Fragments arranged by size: 2626 1890 1059 941 590 528 266 135 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3043 3763 5709 5955 669 Size: 988 281 1105 720 1946 246 3079 Fragments arranged by size: 3079 1946 1105 988 720 281 246 HindIII A'AGCT_T Cuts at: 748 3047 748 Size: 2299 6066 HpaI GTT'AAC Cuts at: 5955 5955 Size: 8365 KpnI G_GTAC'C Cuts at: 2212 4610 5690 2212 Size: 2398 1080 4887 Fragments arranged by size: 4887 2398 1080 MfeI C'AATT_G Cuts at: 2099 2799 5964 2099 Size: 700 3165 4500 Fragments arranged by size: 4500 3165 700 MluI A'CGCG_T Cuts at: 8241 8241 Size: 8365 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4097 4967 7479 7663 1305 Size: 2792 870 2512 184 2007 Fragments arranged by size: 2792 2512 2007 870 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3809 4461 5184 10 Size: 54 1425 343 1977 652 723 3191 Fragments arranged by size: 3191 1977 1425 723 652 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2673 2917 3896 4545 6323 6682 6841 Size: 648 1507 244 979 649 1778 359 159 Cuts at: 6841 518 Size: 2042 Fragments arranged by size: 2042 1778 1507 979 649 648 359 244 159 MspA1I CmG'CkG Cuts at: 1606 2520 2966 4777 5781 6458 7606 7851 1606 Size: 914 446 1811 1004 677 1148 245 2120 Fragments arranged by size: 2120 1811 1148 1004 914 677 446 245 NcoI C'CATG_G Cuts at: 513 1065 4212 4504 5194 513 Size: 552 3147 292 690 3684 Fragments arranged by size: 3684 3147 690 552 292 NdeI CA'TA_TG Cuts at: 387 2450 4593 387 Size: 2063 2143 4159 Fragments arranged by size: 4159 2143 2063 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8365 NotI GC'GGCC_GC Cuts at: 5812 5812 Size: 8365 NsiI A_TGCA'T Cuts at: 2649 4542 5571 2649 Size: 1893 1029 5443 Fragments arranged by size: 5443 1893 1029 NspI r_CATG'y Cuts at: 1132 1229 4071 4116 5230 5300 5723 7268 1132 Size: 97 2842 45 1114 70 423 1545 2229 Fragments arranged by size: 2842 2229 1545 1114 423 97 70 45 PasI CC'CwG_GG Cuts at: 1348 5267 1348 Size: 3919 4446 PciI A'CATG_T Cuts at: 7264 7264 Size: 8365 PflMI CCAn_nnn'nTGG Cuts at: 1108 2589 4457 1108 Size: 1481 1868 5016 Fragments arranged by size: 5016 1868 1481 PfoI T'CCnGG_A Cuts at: 2769 4915 2769 Size: 2146 6219 PmeI GTTT'AAAC Cuts at: 5669 5669 Size: 8365 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2077 2109 4960 4992 6434 6466 7947 7979 2077 Size: 32 2851 32 1442 32 1481 32 2463 Fragments arranged by size: 2851 2463 1481 1442 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 4973 4973 Size: 8365 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8365 PsiI TTA'TAA Cuts at: 2235 5935 2235 Size: 3700 4665 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4912 4944 4912 Size: 32 8333 PstI C_TGCA'G Cuts at: 830 3898 3970 4059 4300 5115 5717 830 Size: 3068 72 89 241 815 602 3478 Fragments arranged by size: 3478 3068 815 602 241 89 72 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8365 PvuII CAG'CTG Cuts at: 2520 2966 4777 2520 Size: 446 1811 6108 Fragments arranged by size: 6108 1811 446 SacI G_AGCT'C Cuts at: 4885 4885 Size: 8365 SalI G'TCGA_C Cuts at: 1936 5707 1936 Size: 3771 4594 SapI GCTCTTCn'nnn_ Cuts at: 3606 3606 Size: 8365 SbfI CC_TGCA'GG Cuts at: 5717 5717 Size: 8365 ScaI AGT'ACT Cuts at: 4225 6613 8325 4225 Size: 2388 1712 4265 Fragments arranged by size: 4265 2388 1712 SfcI C'TryA_G Cuts at: 826 1045 3894 3966 4055 4227 4296 4383 5111 Size: 219 2849 72 89 172 69 87 728 Cuts at: 5111 5713 6848 7529 7720 826 Size: 602 1135 681 191 1471 Fragments arranged by size: 2849 1471 1135 728 681 602 219 191 172 89 87 72 69 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8365 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8365 SmaI CCC'GGG Cuts at: 1872 5692 1872 Size: 3820 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2132 4062 4128 4206 6469 7370 Size: 197 926 189 1930 66 78 2263 901 Cuts at: 7370 7632 820 Size: 262 1553 Fragments arranged by size: 2263 1930 1553 926 901 262 197 189 78 66 SnaBI TAC'GTA Cuts at: 493 6156 493 Size: 5663 2702 SpeI A'CTAG_T Cuts at: 152 4220 152 Size: 4068 4297 SphI G_CATG'C Cuts at: 1132 5230 5723 1132 Size: 4098 493 3774 Fragments arranged by size: 4098 3774 493 SspI AAT'ATT Cuts at: 5 52 2296 4496 5254 6289 5 Size: 47 2244 2200 758 1035 2081 Fragments arranged by size: 2244 2200 2081 1035 758 47 StuI AGG'CCT Cuts at: 1517 3181 4444 4799 1517 Size: 1664 1263 355 5083 Fragments arranged by size: 5083 1664 1263 355 StyI C'CwwG_G Cuts at: 513 1065 2166 2385 4212 4302 4434 4504 5194 Size: 552 1101 219 1827 90 132 70 690 Cuts at: 5194 5804 513 Size: 610 3074 Fragments arranged by size: 3074 1827 1101 690 610 552 219 132 90 70 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4616 5078 5648 5657 6392 6409 6562 Size: 1255 3036 462 570 9 735 17 153 Cuts at: 6562 6747 7192 325 Size: 185 445 1498 Fragments arranged by size: 3036 1498 1255 735 570 462 445 185 153 17 9 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4223 6611 8323 Size: 274 80 33 51 1443 2246 2388 1712 Cuts at: 8323 96 Size: 138 Fragments arranged by size: 2388 2246 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2751 2783 3534 3566 8325 8357 2751 Size: 32 751 32 4759 32 2759 Fragments arranged by size: 4759 2759 751 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8365 XbaI T'CTAG_A Cuts at: 5701 5701 Size: 8365 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2791 5192 5449 2791 Size: 2401 257 5707 Fragments arranged by size: 5707 2401 257 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8365 XmaI C'CCGG_G Cuts at: 1870 5690 1870 Size: 3820 4545 XmnI GAAnn'nnTTC Cuts at: 2399 3241 6494 2399 Size: 842 3253 4270 Fragments arranged by size: 4270 3253 842 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6173 276 Size: 53 83 186 936 4639 2468 Fragments arranged by size: 4639 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BbeI BsmBI BstZ17I Bsu36I FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI