(Circular) (Six-base) MAPSORT of: pFN21AA0901.seq Check: 5410 from: 1 to: 8350 pFN21AA0901 8350 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3117 4305 3117 Size: 1188 7162 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6160 278 Size: 53 83 186 936 4624 2468 Fragments arranged by size: 4624 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5457 5693 1937 Size: 3520 236 4594 Fragments arranged by size: 4594 3520 236 Acc65I G'GTAC_C Cuts at: 3511 5671 3511 Size: 2160 6190 AclI AA'CG_TT Cuts at: 1563 6477 6850 1563 Size: 4914 373 3063 Fragments arranged by size: 4914 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2265 2885 3201 3255 6413 7797 8065 1632 Size: 633 620 316 54 3158 1384 268 1917 Fragments arranged by size: 3158 1917 1384 633 620 316 268 54 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8153 AflIII A'CryG_T Cuts at: 1203 3978 5647 7249 8226 1203 Size: 2775 1669 1602 977 1327 Fragments arranged by size: 2775 1669 1602 1327 977 AgeI A'CCGG_T Cuts at: 7181 7181 Size: 8350 AhdI GACnn_n'nnGTC Cuts at: 7079 7079 Size: 8350 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8350 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8350 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2484 2516 2531 2563 2484 Size: 32 15 32 8271 Fragments arranged by size: 8271 32 32 15 AlwNI CAG_nnn'CTG Cuts at: 2573 3605 4494 6093 7665 2573 Size: 1032 889 1599 1572 3258 Fragments arranged by size: 3258 1599 1572 1032 889 ApaI G_GGCC'C Cuts at: 4128 4453 4128 Size: 325 8025 ApaLI G'TGCA_C Cuts at: 2765 3738 6407 7563 2765 Size: 973 2669 1156 3552 Fragments arranged by size: 3552 2669 1156 973 ApoI r'AATT_y Cuts at: 1493 2291 5503 5659 5885 6037 8146 1493 Size: 798 3212 156 226 152 2109 1697 Fragments arranged by size: 3212 2109 1697 798 226 156 152 AseI AT'TA_AT Cuts at: 160 1032 6904 160 Size: 872 5872 1606 Fragments arranged by size: 5872 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8350 AvaI C'yCGr_G Cuts at: 1870 1943 2999 3672 5077 5675 1870 Size: 73 1056 673 1405 598 4545 Fragments arranged by size: 4545 1405 1056 673 598 73 AvrII C'CTAG_G Cuts at: 4586 4586 Size: 8350 BamHI G'GATC_C Cuts at: 3930 4243 5680 6047 3930 Size: 313 1437 367 6233 Fragments arranged by size: 6233 1437 367 313 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3245 3511 4034 4061 4567 Size: 325 217 627 1458 266 523 27 506 Cuts at: 4567 5048 5671 7126 618 Size: 481 623 1455 1842 Fragments arranged by size: 1842 1458 1455 627 623 523 506 481 325 266 217 27 BanII G_rGCy'C Cuts at: 1391 3303 3402 4128 4453 5669 1391 Size: 1912 99 726 325 1216 4072 Fragments arranged by size: 4072 1912 1216 726 325 99 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2205 4530 928 Size: 971 306 2325 4748 Fragments arranged by size: 4748 2325 971 306 BbvCI CC'TCA_GC Cuts at: 3012 5525 3012 Size: 2513 5837 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4057 4091 5260 5294 6541 6575 1903 Size: 34 2120 34 1169 34 1247 34 3678 Fragments arranged by size: 3678 2120 1247 1169 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6242 7452 6242 Size: 1210 7140 BclI T'GATC_A Cuts at: 1548 4027 5302 1548 Size: 2479 1275 4596 Fragments arranged by size: 4596 2479 1275 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2561 3083 3602 Size: 107 122 71 631 605 889 522 519 Cuts at: 3602 4077 4763 6961 136 Size: 475 686 2198 1525 Fragments arranged by size: 2198 1525 889 686 631 605 522 519 475 122 107 71 BglII A'GATC_T Cuts at: 8345 8345 Size: 8350 BlpI GC'TnA_GC Cuts at: 3606 4596 5767 3606 Size: 990 1171 6189 Fragments arranged by size: 6189 1171 990 Bme1580I G_kGCm'C Cuts at: 1790 2769 3250 3265 3742 4094 4128 4453 5051 Size: 979 481 15 477 352 34 325 598 Cuts at: 5051 6411 7567 1790 Size: 1360 1156 2573 Fragments arranged by size: 2573 1360 1156 979 598 481 477 352 325 34 15 BmgBI CAC'GTC Cuts at: 1318 2980 5389 1318 Size: 1662 2409 4279 Fragments arranged by size: 4279 2409 1662 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2059 2467 2802 Size: 416 53 83 399 24 642 408 335 Cuts at: 2802 3717 3990 5136 6081 7039 442 Size: 915 273 1146 945 958 1753 Fragments arranged by size: 1753 1146 958 945 915 642 416 408 399 335 273 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8350 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2068 3170 3254 3566 4525 4665 5025 5268 Size: 829 1102 84 312 959 140 360 243 Cuts at: 5268 7010 1239 Size: 1742 2579 Fragments arranged by size: 2579 1742 1102 959 829 360 312 243 140 84 Bpu10I CC'TnA_GC Cuts at: 2331 3012 5525 2331 Size: 681 2513 5156 Fragments arranged by size: 5156 2513 681 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2258 3317 6475 7340 7638 2258 Size: 1059 3158 865 298 2970 Fragments arranged by size: 3158 2970 1059 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3210 3326 4219 4398 4463 7013 882 Size: 617 1711 116 893 179 65 2550 2219 Fragments arranged by size: 2550 2219 1711 893 617 179 116 65 BsaAI yAC'GTr Cuts at: 493 1204 2695 3637 6141 493 Size: 711 1491 942 2504 2702 Fragments arranged by size: 2702 2504 1491 942 711 BsaBI GATnn'nnATC Cuts at: 8344 8344 Size: 8350 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3186 6157 6539 7174 Size: 53 83 186 936 1653 2971 382 635 Cuts at: 7174 275 Size: 1451 Fragments arranged by size: 2971 1653 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2613 2736 4846 6783 7181 7455 7602 8013 2613 Size: 123 2110 1937 398 274 147 411 2950 Fragments arranged by size: 2950 2110 1937 411 398 274 147 123 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2531 2561 3689 3719 1178 Size: 30 165 30 1128 30 1128 30 5809 Fragments arranged by size: 5809 1128 1128 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3371 3386 3395 3563 4838 4880 4893 4964 Size: 2185 15 9 168 1275 42 13 71 Cuts at: 4964 4977 5048 5058 5237 1186 Size: 13 71 10 179 4299 Fragments arranged by size: 4299 2185 1275 179 168 71 71 42 15 13 13 10 9 BseYI C'CCAG_C Cuts at: 2655 3096 3516 3553 4249 4804 4930 7553 8049 Size: 441 420 37 696 555 126 2623 496 Cuts at: 8049 2655 Size: 2956 Fragments arranged by size: 2956 2623 696 555 496 441 420 126 37 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2960 3665 4001 4218 4369 5135 1163 Size: 573 1224 705 336 217 151 766 4378 Fragments arranged by size: 4378 1224 766 705 573 336 217 151 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4081 5800 6561 7589 1540 Size: 275 186 2080 1719 761 1028 2301 Fragments arranged by size: 2301 2080 1719 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2769 3286 3411 3742 3786 5084 6411 6496 7567 Size: 517 125 331 44 1298 1327 85 1071 Cuts at: 7567 2769 Size: 3552 Fragments arranged by size: 3552 1327 1298 1071 517 331 125 85 44 BsmI GAATG_Cn' Cuts at: 5861 5954 5861 Size: 93 8257 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2769 3250 3265 3286 3303 3402 3411 Size: 399 979 481 15 21 17 99 9 Cuts at: 3411 3742 3786 4094 4128 4453 5051 5084 5669 Size: 331 44 308 34 325 598 33 585 Cuts at: 5669 6411 6496 7567 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 979 742 598 585 481 399 331 325 308 99 85 44 34 33 21 17 15 9 BspEI T'CCGG_A Cuts at: 2613 2736 4846 8013 2613 Size: 123 2110 3167 2950 Fragments arranged by size: 3167 2950 2110 123 BspHI T'CATG_A Cuts at: 4512 6239 7969 4512 Size: 1727 1730 4893 Fragments arranged by size: 4893 1730 1727 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3117 4305 5380 5705 844 Size: 2273 1188 1075 325 3489 Fragments arranged by size: 3489 2273 1188 1075 325 BsrBI CCG'CTC Cuts at: 1394 1823 2130 2335 4600 6237 1394 Size: 429 307 205 2265 1637 3507 Fragments arranged by size: 3507 2265 1637 429 307 205 BsrDI GCAATG_nn' Cuts at: 65 1248 2196 2296 2961 4179 6845 7019 65 Size: 1183 948 100 665 1218 2666 174 1396 Fragments arranged by size: 2666 1396 1218 1183 948 665 174 100 BsrFI r'CCGG_y Cuts at: 1700 1810 2015 6994 7181 1700 Size: 110 205 4979 187 2869 Fragments arranged by size: 4979 2869 205 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8350 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8350 BssSI C'ACGA_G Cuts at: 4611 6410 7422 4611 Size: 1799 1012 5539 Fragments arranged by size: 5539 1799 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2983 3746 794 Size: 2189 763 5398 Fragments arranged by size: 5398 2189 763 BstBI TT'CG_AA Cuts at: 2096 7154 2096 Size: 5058 3292 BstEII G'GTnAC_C Cuts at: 1184 4555 1184 Size: 3371 4979 BstXI CCAn_nnnn'nTGG Cuts at: 3965 5337 5402 5498 3965 Size: 1372 65 96 6817 Fragments arranged by size: 6817 1372 96 65 BstYI r'GATC_y Cuts at: 1972 2227 2610 3930 4243 5285 5680 6047 6432 Size: 255 383 1320 313 1042 395 367 385 Cuts at: 6432 6449 7901 7987 7999 8345 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1320 1042 395 385 383 367 346 313 255 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2076 2566 2579 3507 4272 2076 Size: 490 13 928 765 6154 Fragments arranged by size: 6154 928 765 490 13 BtgI C'CryG_G Cuts at: 513 1065 2770 3336 3958 4047 4374 4430 4998 Size: 552 1705 566 622 89 327 56 568 Cuts at: 4998 5497 513 Size: 499 3366 Fragments arranged by size: 3366 1705 622 568 566 552 499 327 89 56 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4148 4469 5590 487 Size: 683 56 2922 321 1121 3247 Fragments arranged by size: 3247 2922 1121 683 321 56 BtsI GCAGTG_nn' Cuts at: 3478 5330 5866 6660 6680 3478 Size: 1852 536 794 20 5148 Fragments arranged by size: 5148 1852 794 536 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8350 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8315 DraI TTT'AAA Cuts at: 5654 5654 Size: 8350 DraIII CAC_nnn'GTG Cuts at: 3245 3245 Size: 8350 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2087 2810 7357 809 Size: 507 771 723 4547 1802 Fragments arranged by size: 4547 1802 771 723 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2017 2182 2625 Size: 54 1425 50 275 18 187 165 443 Cuts at: 2625 2896 3072 3425 4078 4318 5479 5797 6686 Size: 271 176 353 653 240 1161 318 889 Cuts at: 6686 8315 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1161 889 653 443 353 318 275 271 240 187 176 165 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4078 5797 1537 Size: 275 2266 1719 4090 Fragments arranged by size: 4090 2266 1719 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2415 4665 6280 1353 Size: 1062 2250 1615 3423 Fragments arranged by size: 3423 2250 1615 1062 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6937 7311 7457 387 Size: 1064 138 5348 374 146 1280 Fragments arranged by size: 5348 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2068 2265 2885 3170 3201 3254 3255 Size: 393 436 197 620 285 31 53 1 Cuts at: 3255 3566 4525 4665 5025 5268 6413 7010 7797 Size: 311 959 140 360 243 1145 597 787 Cuts at: 7797 8065 1239 Size: 268 1524 Fragments arranged by size: 1524 1145 959 787 620 597 436 393 360 311 285 268 243 197 140 53 31 1 EcoNI CCTnn'n_nnAGG Cuts at: 3773 4721 5376 3773 Size: 948 655 6747 Fragments arranged by size: 6747 948 655 EcoO109I rG'GnC_Cy Cuts at: 2327 2371 2743 2803 3055 3389 4125 4449 4450 Size: 44 372 60 252 334 736 324 1 Cuts at: 4450 4535 5166 5196 5212 5293 2327 Size: 85 631 30 16 81 5384 Fragments arranged by size: 5384 736 631 372 334 324 252 85 81 60 44 30 16 1 EcoRI G'AATT_C Cuts at: 2291 5659 6037 2291 Size: 3368 378 4604 Fragments arranged by size: 4604 3368 378 EcoRV GAT'ATC Cuts at: 5136 5136 Size: 8350 FspI TGC'GCA Cuts at: 6856 6856 Size: 8350 HaeII r_GCGC'y Cuts at: 1678 3571 3759 7497 1678 Size: 1893 188 3738 2531 Fragments arranged by size: 3738 2531 1893 188 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2901 2933 3130 3162 4718 Size: 32 135 32 1329 32 197 32 1556 Cuts at: 4718 4750 5050 5082 5143 5175 6991 7023 7065 Size: 32 300 32 61 32 1816 32 42 Cuts at: 7065 7097 1373 Size: 32 2626 Fragments arranged by size: 2626 1816 1556 1329 300 197 135 61 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4108 5694 5940 669 Size: 988 281 2170 1586 246 3079 Fragments arranged by size: 3079 2170 1586 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8350 HpaI GTT'AAC Cuts at: 5940 5940 Size: 8350 KpnI G_GTAC'C Cuts at: 3515 5675 3515 Size: 2160 6190 MfeI C'AATT_G Cuts at: 5949 5949 Size: 8350 MluI A'CGCG_T Cuts at: 8226 8226 Size: 8350 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2253 3720 5515 7464 7648 1305 Size: 948 1467 1795 1949 184 2007 Fragments arranged by size: 2007 1949 1795 1467 948 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2627 3427 5481 10 Size: 54 1425 343 795 800 2054 2879 Fragments arranged by size: 2879 2054 1425 800 795 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2921 3963 4023 6308 6667 6826 518 Size: 648 1755 1042 60 2285 359 159 2042 Fragments arranged by size: 2285 2042 1755 1042 648 359 159 60 MspA1I CmG'CkG Cuts at: 1606 3016 4220 4249 4706 5246 5518 5766 6443 Size: 1410 1204 29 457 540 272 248 677 Cuts at: 6443 7591 7836 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1410 1204 1148 677 540 457 272 248 245 29 NcoI C'CATG_G Cuts at: 513 1065 4047 4374 4998 5497 513 Size: 552 2982 327 624 499 3366 Fragments arranged by size: 3366 2982 624 552 499 327 NdeI CA'TA_TG Cuts at: 387 387 Size: 8350 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8350 NotI GC'GGCC_GC Cuts at: 5797 5797 Size: 8350 NspI r_CATG'y Cuts at: 1132 1229 2686 3923 3982 5488 5708 7253 1132 Size: 97 1457 1237 59 1506 220 1545 2229 Fragments arranged by size: 2229 1545 1506 1457 1237 220 97 59 PasI CC'CwG_GG Cuts at: 1348 3393 1348 Size: 2045 6305 PciI A'CATG_T Cuts at: 3978 7249 3978 Size: 3271 5079 PflMI CCAn_nnn'nTGG Cuts at: 1108 2191 5353 1108 Size: 1083 3162 4105 Fragments arranged by size: 4105 3162 1083 PfoI T'CCnGG_A Cuts at: 2319 2364 2641 2319 Size: 45 277 8028 Fragments arranged by size: 8028 277 45 PmeI GTTT'AAAC Cuts at: 5654 5654 Size: 8350 PmlI CAC'GTG Cuts at: 2695 2695 Size: 8350 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2531 2563 6419 6451 7932 7964 2531 Size: 32 3856 32 1481 32 2917 Fragments arranged by size: 3856 2917 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2743 2803 3055 3389 4535 5166 2743 Size: 60 252 334 1146 631 5927 Fragments arranged by size: 5927 1146 631 334 252 60 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8350 PsiI TTA'TAA Cuts at: 5920 5920 Size: 8350 PspOMI G'GGCC_C Cuts at: 4124 4449 4124 Size: 325 8025 PspXI vC'TCGA_Gb Cuts at: 2999 5077 2999 Size: 2078 6272 PstI C_TGCA'G Cuts at: 830 5377 5702 830 Size: 4547 325 3478 Fragments arranged by size: 4547 3478 325 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8350 PvuII CAG'CTG Cuts at: 3016 4220 4706 5246 3016 Size: 1204 486 540 6120 Fragments arranged by size: 6120 1204 540 486 SalI G'TCGA_C Cuts at: 1936 5692 1936 Size: 3756 4594 SanDI GG'GwC_CC Cuts at: 3055 3055 Size: 8350 SapI GCTCTTCn'nnn_ Cuts at: 2415 2415 Size: 8350 SbfI CC_TGCA'GG Cuts at: 5702 5702 Size: 8350 ScaI AGT'ACT Cuts at: 6598 8310 6598 Size: 1712 6638 SexAI A'CCwGG_T Cuts at: 2934 3241 2934 Size: 307 8043 SfcI C'TryA_G Cuts at: 826 1045 3434 4956 5373 5698 6833 7514 7705 Size: 219 2389 1522 417 325 1135 681 191 Cuts at: 7705 826 Size: 1471 Fragments arranged by size: 2389 1522 1471 1135 681 417 325 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8350 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8350 SmaI CCC'GGG Cuts at: 1872 3674 5677 1872 Size: 1802 2003 4545 Fragments arranged by size: 4545 2003 1802 SmlI C'TyrA_G Cuts at: 820 1017 1943 2237 2999 3296 5077 6454 7355 Size: 197 926 294 762 297 1781 1377 901 Cuts at: 7355 7617 820 Size: 262 1553 Fragments arranged by size: 1781 1553 1377 926 901 762 297 294 262 197 SnaBI TAC'GTA Cuts at: 493 6141 493 Size: 5648 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8350 SphI G_CATG'C Cuts at: 1132 3923 5708 1132 Size: 2791 1785 3774 Fragments arranged by size: 3774 2791 1785 SspI AAT'ATT Cuts at: 5 52 2107 6274 5 Size: 47 2055 4167 2081 Fragments arranged by size: 4167 2081 2055 47 StuI AGG'CCT Cuts at: 1517 2638 2870 5382 5567 1517 Size: 1121 232 2512 185 4300 Fragments arranged by size: 4300 2512 1121 232 185 StyI C'CwwG_G Cuts at: 513 1065 2899 2905 2927 3060 4047 4374 4399 Size: 552 1834 6 22 133 987 327 25 Cuts at: 4399 4586 4998 5169 5497 5789 513 Size: 187 412 171 328 292 3074 Fragments arranged by size: 3074 1834 987 552 412 328 327 292 187 171 133 25 22 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4124 4307 5422 5658 6377 6394 6547 Size: 1255 2544 183 1115 236 719 17 153 Cuts at: 6547 6732 7177 325 Size: 185 445 1498 Fragments arranged by size: 2544 1498 1255 1115 719 445 236 185 183 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2475 5144 6596 Size: 274 80 33 51 1443 498 2669 1452 Cuts at: 6596 8308 96 Size: 1712 138 Fragments arranged by size: 2669 1712 1452 1443 498 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 8310 8342 8310 Size: 32 8318 Tth111I GACn'n_nGTC Cuts at: 1316 5026 1316 Size: 3710 4640 XbaI T'CTAG_A Cuts at: 5686 5686 Size: 8350 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3067 3420 3911 3962 4549 5504 5621 3067 Size: 353 491 51 587 955 117 5796 Fragments arranged by size: 5796 955 587 491 353 117 51 XhoI C'TCGA_G Cuts at: 1943 2999 5077 1943 Size: 1056 2078 5216 Fragments arranged by size: 5216 2078 1056 XmaI C'CCGG_G Cuts at: 1870 3672 5675 1870 Size: 1802 2003 4545 Fragments arranged by size: 4545 2003 1802 XmnI GAAnn'nnTTC Cuts at: 6479 6479 Size: 8350 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6158 276 Size: 53 83 186 936 4624 2468 Fragments arranged by size: 4624 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I EcoICRI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PsrI RsrII SacI SacII SfiI SfoI SrfI SwaI