Restriction map for
pFN21AA0892
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# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII AccIII AclI AcyI AflII
AgeI AocI Aor51HI ApaI AscI
AseI AsnI Asp700I AspI AvrII
BamHI BbeI BcgI BciVI BclI
BfaI BfrI BlnI BsaHI BsaWI
BseAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BsrBI BsrGI
Bst1107I BstXI BstZ17I Bsu36I ClaI
CpoI DraI DraIII DrdI Eco47III
Eco81I EcoT22I FbaI FseI Hin1I
HindIII HpaI KasI KspI MaeI
MfeI MluI MroI MunI NarI
NdeI NheI NotI NruI NsiI
PacI PaeR7I PflFI PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI ScaI
SfoI SgrAI SnaBI SpeI SphI
SspBI SspI SwaI TfiI Tth111I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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Acc65I 74 GGTACC G^GTACC
AccI 874 GTCTAC GT^[AC][GT]AC
Asp718I 74 GGTACC G^GTACC
AvaI 524 CCCGGG C^[CT]CG[GA]G
AviII 48 TGCGCA TGC^GCA
BalI 1826 TGGCCA TGG^CCA
BbrPI 1764 CACGTG CAC^GTG
BbsI 688 GTCTTC GAAGAC(2/6)
BlpI 97 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 97 GCTGAGC GC^T[ACGT]AGC
BpuAI 688 GTCTTC GAAGAC(2/6)
BsaAI 1764 CACGTG [CT]AC^GT[GA]
BsaBI 399 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1637 GAGACC GGTCTC(1/5)
BseRI 562 CTCCTC GAGGAG(10/8)
BsiEI 62 CGGCCG CG[GA][CT]^CG
BsoBI 524 CCCGGG C^[CT]CG[GA]G
BsrDI 1148 CATTGC GCAATG(2/0)
BssHII 253 GCGCGC G^CGCGC
BstBI 304 TTCGAA TT^CGAA
BstEII 708 GGTCACC G^GT[ACGT]ACC
BstPI 708 GGTCACC G^GT[ACGT]ACC
CelII 97 GCTGAGC GC^T[ACGT]AGC
EagI 62 CGGCCG C^GGCCG
EclXI 62 CGGCCG C^GGCCG
Eco52I 62 CGGCCG C^GGCCG
EcoO65I 708 GGTCACC G^GT[ACGT]ACC
EcoRI 360 GAATTC G^AATTC
EcoRV 282 GATATC GAT^ATC
FspI 48 TGCGCA TGC^GCA
HgaI 1418 GACGC GACGC(5/10)
HincII 1174 GTCAAC GT[CT]^[GA]AC
HindII 1174 GTCAAC GT[CT]^[GA]AC
KpnI 74 GGTACC GGTAC^C
MaeII 1765 ACGT A^CGT
MamI 399 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1826 TGGCCA TGG^CCA
MscI 1826 TGGCCA TGG^CCA
MseI 314 TTAA T^TAA
NaeI 907 GCCGGC GCC^GGC
NcoI 1664 CCATGG C^CATGG
NgoMIV 907 GCCGGC G^CCGGC
NspV 304 TTCGAA TT^CGAA
PflMI 714 CCACTATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1764 CACGTG CAC^GTG
PmlI 1764 CACGTG CAC^GTG
SbfI 150 CCTGCAGG CCTGCA^GG
SfiI 54 GGCCGAGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 304 TTCGAA TT^CGAA
SmaI 524 CCCGGG CCC^GGG
Sse8387I 150 CCTGCAGG CCTGCA^GG
StuI 1733 AGGCCT AGG^CCT
Tru9I 314 TTAA T^TAA
Van91I 714 CCACTATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 524 CCCGGG C^CCGGG
XmaI 524 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 36 enzymes) #
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AccII 254 CGCG CG^CG
1191 CGCG
AcsI 316 AAATTT [GA]^AATT[CT]
360 GAATTC
AflIII 860 ACATGT A^C[GA][CT]GT
1601 ACATGT
AhdI 478 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1770 GACAGACGGTC
Alw44I 142 GTGCAC G^TGCAC
610 GTGCAC
AlwNI 1234 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1708 CAGCAGCTG
ApaLI 142 GTGCAC G^TGCAC
610 GTGCAC
ApoI 316 AAATTT [GA]^AATT[CT]
360 GAATTC
AspEI 478 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1770 GACAGACGGTC
BanI 74 GGTACC G^G[CT][GA]CC
1566 GGTGCC
BanII 213 GGGCTC G[GA]GC[CT]^C
1396 GGGCTC
BbvCI 564 CCTCAGC CCTCAGC(-5/-2)
1471 GCTGAGG
BglI 25 GCCCAGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
55 GCCGAGGCGGC
BglII 404 AGATCT A^GATCT
1076 AGATCT
BsmAI 1637 GAGAC GTCTC(1/5)
1168 GTCTC
BsmFI 1416 GGGAC GGGAC(10/14)
1107 GTCCC
BspLU11I 860 ACATGT A^CATGT
1601 ACATGT
BspMI 203 ACCTGC ACCTGC(4/8)
732 GCAGGT
BsrFI 907 GCCGGC [GA]^CCGG[CT]
1484 ACCGGC
BssSI 69 CTCGTG CACGAG(-5/-1)
1504 CTCGTG
BstUI 254 CGCG CG^CG
1191 CGCG
Cfr10I 907 GCCGGC [GA]^CCGG[CT]
1484 ACCGGC
EaeI 62 CGGCCG [CT]^GGCC[GA]
1826 TGGCCA
Eam1105I 478 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1770 GACAGACGGTC
EcoT14I 851 CCAAGG C^C[AT][AT]GG
1664 CCATGG
HinfI 67 GACTC G^A[ACGT]TC
676 GAGTC
MvnI 254 CGCG CG^CG
1191 CGCG
NspI 860 ACATGT [GA]CATG^[CT]
1601 ACATGT
PleI 67 GACTC GAGTC(4/5)
676 GAGTC
PpuMI 476 AGGACCT [GA]G^G[AT]CC[CT]
1475 AGGACCT
SexAI 479 ACCTGGT A^CC[AT]GGT
878 ACCTGGT
SmlI 469 CTTGAG C^T[CT][GA]AG
967 CTCAAG
StyI 851 CCAAGG C^C[AT][AT]GG
1664 CCATGG
TaqI 188 TCGA T^CGA
305 TCGA
TthHB8I 188 TCGA T^CGA
305 TCGA
XcmI 1797 CCAAGCTCAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1814 CCAACACCAGCCTGG
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# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaII BbvI BcnI BmyI BpmI
BsaJI BsgI BsiHKAI BsiYI BslI
Bsp1286I BsrI BssKI BstAPI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EarI
EcoNI EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HpaII HphI ItaI Ksp632I
MaeIII MboI MboII MflI MnlI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PstI
PvuII RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI TseI Tsp45I
Tsp509I TspRI XhoII