(Circular) (Six-base) MAPSORT of: pFN21AA0892.seq Check: 8469 from: 1 to: 6544 pFN21AA0892 6544 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2217 2728 2217 Size: 511 6033 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4354 278 Size: 53 83 186 936 2818 2468 Fragments arranged by size: 2818 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2880 3887 1937 Size: 943 1007 4594 Fragments arranged by size: 4594 1007 943 Acc65I G'GTAC_C Cuts at: 2079 3865 2079 Size: 1786 4758 AclI AA'CG_TT Cuts at: 1563 4671 5044 1563 Size: 3108 373 3063 Fragments arranged by size: 3108 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2783 3482 4607 5991 6259 1632 Size: 1151 699 1125 1384 268 1917 Fragments arranged by size: 1917 1384 1151 1125 699 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6347 AflIII A'CryG_T Cuts at: 1203 2865 3606 5443 6420 1203 Size: 1662 741 1837 977 1327 Fragments arranged by size: 1837 1662 1327 977 741 AgeI A'CCGG_T Cuts at: 5375 5375 Size: 6544 AhdI GACnn_n'nnGTC Cuts at: 2488 3780 5273 2488 Size: 1292 1493 3759 Fragments arranged by size: 3759 1493 1292 AleI CACnn'nnGTG Cuts at: 1166 2868 1166 Size: 1702 4842 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2597 1184 Size: 1413 5131 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3191 3223 3191 Size: 32 6512 AlwNI CAG_nnn'CTG Cuts at: 3244 3718 4287 5859 3244 Size: 474 569 1572 3929 Fragments arranged by size: 3929 1572 569 474 ApaLI G'TGCA_C Cuts at: 2147 2615 4601 5757 2147 Size: 468 1986 1156 2934 Fragments arranged by size: 2934 1986 1156 468 ApoI r'AATT_y Cuts at: 1493 2321 2365 3853 4079 4231 6340 1493 Size: 828 44 1488 226 152 2109 1697 Fragments arranged by size: 2109 1697 1488 828 226 152 44 AseI AT'TA_AT Cuts at: 160 1032 5098 160 Size: 872 4066 1606 Fragments arranged by size: 4066 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6544 AvaI C'yCGr_G Cuts at: 1870 1943 2529 3869 1870 Size: 73 586 1340 4545 Fragments arranged by size: 4545 1340 586 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3605 3638 3605 Size: 33 6511 BamHI G'GATC_C Cuts at: 3874 4241 3874 Size: 367 6177 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2079 3571 3865 5320 618 Size: 325 217 627 292 1492 294 1455 1842 Fragments arranged by size: 1842 1492 1455 627 325 294 292 217 BanII G_rGCy'C Cuts at: 1391 2222 3405 3863 1391 Size: 831 1183 458 4072 Fragments arranged by size: 4072 1183 831 458 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2686 928 Size: 971 787 4786 Fragments arranged by size: 4786 971 787 BbvCI CC'TCA_GC Cuts at: 2570 3477 2570 Size: 907 5637 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4735 4769 1903 Size: 34 2798 34 3678 Fragments arranged by size: 3678 2798 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4436 5646 4436 Size: 1210 5334 BclI T'GATC_A Cuts at: 1548 1548 Size: 6544 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2036 2066 Size: 107 122 71 631 605 337 27 30 Cuts at: 2066 5155 136 Size: 3089 1525 Fragments arranged by size: 3089 1525 631 605 337 122 107 71 30 27 BglII A'GATC_T Cuts at: 2409 3081 6539 2409 Size: 672 3458 2414 Fragments arranged by size: 3458 2414 672 BlpI GC'TnA_GC Cuts at: 2103 3961 2103 Size: 1858 4686 Bme1580I G_kGCm'C Cuts at: 1790 2151 2619 4605 5761 1790 Size: 361 468 1986 1156 2573 Fragments arranged by size: 2573 1986 1156 468 361 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6544 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3199 4275 5233 Size: 416 53 83 399 24 1782 1076 958 Cuts at: 5233 442 Size: 1753 Fragments arranged by size: 1782 1753 1076 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6544 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2291 2693 2942 2984 3044 3347 3707 5204 Size: 1052 402 249 42 60 303 360 1497 Cuts at: 5204 1239 Size: 2579 Fragments arranged by size: 2579 1497 1052 402 360 303 249 60 42 Bpu10I CC'TnA_GC Cuts at: 2013 2570 3477 2013 Size: 557 907 5080 Fragments arranged by size: 5080 907 557 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2495 2957 4669 5534 5832 2495 Size: 462 1712 865 298 3207 Fragments arranged by size: 3207 1712 865 462 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3636 5207 882 Size: 617 2137 1571 2219 Fragments arranged by size: 2219 2137 1571 617 BsaAI yAC'GTr Cuts at: 493 1204 3771 4335 493 Size: 711 2567 564 2702 Fragments arranged by size: 2702 2567 711 564 BsaBI GATnn'nnATC Cuts at: 2408 6538 2408 Size: 4130 2414 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4351 4733 5368 275 Size: 53 83 186 936 2818 382 635 1451 Fragments arranged by size: 2818 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4977 5375 5649 5796 6207 4977 Size: 398 274 147 411 5314 Fragments arranged by size: 5314 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2264 2294 2852 2882 1178 Size: 30 165 30 861 30 558 30 4840 Fragments arranged by size: 4840 861 558 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2558 1186 Size: 1372 5172 BseYI C'CCAG_C Cuts at: 2215 2822 3157 3538 5747 6243 2215 Size: 607 335 381 2209 496 2516 Fragments arranged by size: 2516 2209 607 496 381 335 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2450 2786 3131 3815 1163 Size: 573 714 336 345 684 3892 Fragments arranged by size: 3892 714 684 573 345 336 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2070 3994 4755 5783 1540 Size: 275 186 69 1924 761 1028 2301 Fragments arranged by size: 2301 1924 1028 761 275 186 69 BsiHKAI G_wGCw'C Cuts at: 2109 2151 2619 2709 2865 3030 4605 4690 5761 Size: 42 468 90 156 165 1575 85 1071 Cuts at: 5761 2109 Size: 2892 Fragments arranged by size: 2892 1575 1071 468 165 156 90 85 42 BsmI GAATG_Cn' Cuts at: 4055 4148 4055 Size: 93 6451 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2109 2151 2222 2619 2709 2865 3030 Size: 399 319 42 71 397 90 156 165 Cuts at: 3030 3405 3863 4605 4690 5761 1391 Size: 375 458 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 458 399 397 375 319 165 156 90 85 71 42 BspEI T'CCGG_A Cuts at: 6207 6207 Size: 6544 BspHI T'CATG_A Cuts at: 4433 6163 4433 Size: 1730 4814 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2217 2728 3899 844 Size: 1373 511 1171 3489 Fragments arranged by size: 3489 1373 1171 511 BsrBI CCG'CTC Cuts at: 1394 1823 4431 1394 Size: 429 2608 3507 Fragments arranged by size: 3507 2608 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3152 5039 5213 65 Size: 1183 1904 1887 174 1396 Fragments arranged by size: 1904 1887 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2912 3489 5188 5375 1700 Size: 110 1102 577 1699 187 2869 Fragments arranged by size: 2869 1699 1102 577 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6544 BssHII G'CGCG_C Cuts at: 1925 2258 1925 Size: 333 6211 BssSI C'ACGA_G Cuts at: 2074 3509 4604 5616 2074 Size: 1435 1095 1012 3002 Fragments arranged by size: 3002 1435 1095 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2155 2614 2623 2905 3154 3718 794 Size: 1361 459 9 282 249 564 3620 Fragments arranged by size: 3620 1361 564 459 282 249 9 BstBI TT'CG_AA Cuts at: 2310 5348 2310 Size: 3038 3506 BstEII G'GTnAC_C Cuts at: 1184 2713 1184 Size: 1529 5015 BstXI CCAn_nnnn'nTGG Cuts at: 3841 3841 Size: 6544 BstYI r'GATC_y Cuts at: 1972 2409 2540 2814 3081 3597 3874 4241 4626 Size: 437 131 274 267 516 277 367 385 Cuts at: 4626 4643 6095 6181 6193 6539 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 516 437 385 367 346 277 274 267 131 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3066 3213 3669 513 Size: 552 939 1062 147 456 3388 Fragments arranged by size: 3388 1062 939 552 456 147 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5805 Fragments arranged by size: 5805 683 56 BtsI GCAGTG_nn' Cuts at: 2149 2434 2783 2798 3367 3632 4060 4854 4874 Size: 285 349 15 569 265 428 794 20 Cuts at: 4874 2149 Size: 3819 Fragments arranged by size: 3819 794 569 428 349 285 265 20 15 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6544 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6509 DraI TTT'AAA Cuts at: 3848 3848 Size: 6544 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5551 809 Size: 507 4235 1802 Fragments arranged by size: 4235 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2067 3831 3991 Size: 54 1425 50 275 18 237 1764 160 Cuts at: 3991 4880 6509 8 Size: 889 1629 43 Fragments arranged by size: 1764 1629 1425 889 275 237 160 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2067 3991 1537 Size: 275 255 1924 4090 Fragments arranged by size: 4090 1924 275 255 EarI CTCTTCn'nnn_ Cuts at: 1353 2453 2738 2846 3128 3410 4474 1353 Size: 1100 285 108 282 282 1064 3423 Fragments arranged by size: 3423 1100 1064 285 282 282 108 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2126 5131 5505 5651 387 Size: 1064 138 537 3005 374 146 1280 Fragments arranged by size: 3005 1280 1064 537 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2291 2693 2783 2942 2984 3044 3347 Size: 393 659 402 90 159 42 60 303 Cuts at: 3347 3482 3707 4607 5204 5991 6259 1239 Size: 135 225 900 597 787 268 1524 Fragments arranged by size: 1524 900 787 659 597 402 393 303 268 225 159 135 90 60 42 EcoNI CCTnn'n_nnAGG Cuts at: 2273 2573 2924 2273 Size: 300 351 5893 Fragments arranged by size: 5893 351 300 EcoO109I rG'GnC_Cy Cuts at: 2482 2950 3481 2482 Size: 468 531 5545 Fragments arranged by size: 5545 531 468 EcoRI G'AATT_C Cuts at: 2365 3853 4231 2365 Size: 1488 378 4678 Fragments arranged by size: 4678 1488 378 EcoRV GAT'ATC Cuts at: 2289 2289 Size: 6544 FspI TGC'GCA Cuts at: 2055 5050 2055 Size: 2995 3549 HaeII r_GCGC'y Cuts at: 1678 2090 2561 3074 5691 1678 Size: 412 471 513 2617 2531 Fragments arranged by size: 2617 2531 513 471 412 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2474 2506 3766 3798 5185 Size: 32 135 32 902 32 1260 32 1387 Cuts at: 5185 5217 5259 5291 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1387 1260 902 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3181 3888 4134 669 Size: 988 281 1243 707 246 3079 Fragments arranged by size: 3079 1243 988 707 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6544 HpaI GTT'AAC Cuts at: 4134 4134 Size: 6544 KpnI G_GTAC'C Cuts at: 2083 3869 2083 Size: 1786 4758 MfeI C'AATT_G Cuts at: 4143 4143 Size: 6544 MluI A'CGCG_T Cuts at: 6420 6420 Size: 6544 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3153 5658 5842 1305 Size: 1848 2505 184 2007 Fragments arranged by size: 2505 2007 1848 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3833 10 Size: 54 1425 343 2001 2721 Fragments arranged by size: 2721 2001 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2808 2868 2870 3035 3569 4502 4861 Size: 648 1642 60 2 165 534 933 359 Cuts at: 4861 5020 518 Size: 159 2042 Fragments arranged by size: 2042 1642 933 648 534 359 165 159 60 2 MspA1I CmG'CkG Cuts at: 1606 2027 2215 2389 2623 2770 2834 2962 3100 Size: 421 188 174 234 147 64 128 138 Cuts at: 3100 3145 3619 3718 3960 4637 5785 6030 1606 Size: 45 474 99 242 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 474 421 245 242 234 188 174 147 138 128 99 64 45 NaeI GCC'GGC Cuts at: 2914 2914 Size: 6544 NcoI C'CATG_G Cuts at: 513 1065 2004 3669 513 Size: 552 939 1665 3388 Fragments arranged by size: 3388 1665 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6544 NgoMIV G'CCGG_C Cuts at: 2912 2912 Size: 6544 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6544 NotI GC'GGCC_GC Cuts at: 3991 3991 Size: 6544 NspI r_CATG'y Cuts at: 1132 1229 2869 3610 3902 5447 1132 Size: 97 1640 741 292 1545 2229 Fragments arranged by size: 2229 1640 1545 741 292 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6544 PciI A'CATG_T Cuts at: 2865 3606 5443 2865 Size: 741 1837 3966 Fragments arranged by size: 3966 1837 741 PflMI CCAn_nnn'nTGG Cuts at: 1108 2725 1108 Size: 1617 4927 PfoI T'CCnGG_A Cuts at: 3600 3723 3600 Size: 123 6421 PmeI GTTT'AAAC Cuts at: 3848 3848 Size: 6544 PmlI CAC'GTG Cuts at: 3771 3771 Size: 6544 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3747 3779 4613 4645 6126 6158 3747 Size: 32 834 32 1481 32 4133 Fragments arranged by size: 4133 1481 834 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2482 3481 2482 Size: 999 5545 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6544 PsiI TTA'TAA Cuts at: 4114 4114 Size: 6544 PstI C_TGCA'G Cuts at: 830 2160 2214 2610 2775 2990 3078 3746 3896 Size: 1330 54 396 165 215 88 668 150 Cuts at: 3896 830 Size: 3478 Fragments arranged by size: 3478 1330 668 396 215 165 150 88 54 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6544 PvuII CAG'CTG Cuts at: 2215 2770 2962 3100 3145 3619 3718 2215 Size: 555 192 138 45 474 99 5041 Fragments arranged by size: 5041 555 474 192 138 99 45 SalI G'TCGA_C Cuts at: 1936 3886 1936 Size: 1950 4594 SapI GCTCTTCn'nnn_ Cuts at: 2453 2738 3128 3410 2453 Size: 285 390 282 5587 Fragments arranged by size: 5587 390 285 282 SbfI CC_TGCA'GG Cuts at: 2160 3896 2160 Size: 1736 4808 ScaI AGT'ACT Cuts at: 4792 6504 4792 Size: 1712 4832 SexAI A'CCwGG_T Cuts at: 2484 2883 2484 Size: 399 6145 SfcI C'TryA_G Cuts at: 826 1045 2156 2210 2606 2771 2986 3074 3316 Size: 219 1111 54 396 165 215 88 242 Cuts at: 3316 3742 3892 5027 5708 5899 826 Size: 426 150 1135 681 191 1471 Fragments arranged by size: 1471 1135 1111 681 426 396 242 219 215 191 165 150 88 54 SfiI GGCCn_nnn'nGGCC Cuts at: 2066 2066 Size: 6544 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6544 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6544 SmaI CCC'GGG Cuts at: 1872 2531 3871 1872 Size: 659 1340 4545 Fragments arranged by size: 4545 1340 659 SmlI C'TyrA_G Cuts at: 820 1017 1943 2474 2972 4648 5549 5811 820 Size: 197 926 531 498 1676 901 262 1553 Fragments arranged by size: 1676 1553 926 901 531 498 262 197 SnaBI TAC'GTA Cuts at: 493 4335 493 Size: 3842 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6544 SphI G_CATG'C Cuts at: 1132 3902 1132 Size: 2770 3774 SspI AAT'ATT Cuts at: 5 52 4468 5 Size: 47 4416 2081 Fragments arranged by size: 4416 2081 47 StuI AGG'CCT Cuts at: 1517 3740 1517 Size: 2223 4321 StyI C'CwwG_G Cuts at: 513 1065 2004 2856 3669 3983 513 Size: 552 939 852 813 314 3074 Fragments arranged by size: 3074 939 852 813 552 314 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2498 2732 4571 4588 4741 4926 5371 Size: 1255 918 234 1839 17 153 185 445 Cuts at: 5371 325 Size: 1498 Fragments arranged by size: 1839 1498 1255 918 445 234 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2352 2937 3165 Size: 274 80 33 51 1443 375 585 228 Cuts at: 3165 4790 6502 96 Size: 1625 1712 138 Fragments arranged by size: 1712 1625 1443 585 375 274 228 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2264 2283 2296 2315 2852 2884 6504 6536 2264 Size: 19 13 19 537 32 3620 32 2272 Fragments arranged by size: 3620 2272 537 32 32 19 19 13 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6544 XbaI T'CTAG_A Cuts at: 3880 3880 Size: 6544 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3809 3826 3809 Size: 17 6527 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6544 XmaI C'CCGG_G Cuts at: 1870 2529 3869 1870 Size: 659 1340 4545 Fragments arranged by size: 4545 1340 659 XmnI GAAnn'nnTTC Cuts at: 4673 4673 Size: 6544 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4352 276 Size: 53 83 186 936 2818 2468 Fragments arranged by size: 2818 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI FalI FseI FspAI KasI NarI NruI NsiI PacI PspOMI PspXI PsrI RsrII SacI SacII SanDI SfoI SrfI SwaI