Restriction map for
pFN21AA0890
(Circular) (Six-base) MAPSORT of: pFN21AA0890.seq Check: 9606 from: 1 to: 8287
pFN21AA0890 8287 bp
With 182 enzymes: SgfI *
January 16, 2009 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6097 278
Size: 53 83 186 936 4561 2468
Fragments arranged by size:
4561 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2229 2837 5183 5630 1937
Size: 292 608 2346 447 4594
Fragments arranged by size:
4594 2346 608 447 292
Acc65I G'GTAC_C
Cuts at: 5608 5608
Size: 8287
AclI AA'CG_TT
Cuts at: 1563 6414 6787 1563
Size: 4851 373 3063
Fragments arranged by size:
4851 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2960 4301 4720 5438 6350 7734 8002 1632
Size: 1328 1341 419 718 912 1384 268 1917
Fragments arranged by size:
1917 1384 1341 1328 912 718 419 268
AfeI AGC'GCT
Cuts at: 3425 3797 3425
Size: 372 7915
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8090
AflIII A'CryG_T
Cuts at: 1203 7186 8163 1203
Size: 5983 977 1327
Fragments arranged by size:
5983 1327 977
AgeI A'CCGG_T
Cuts at: 7118 7118
Size: 8287
AhdI GACnn_n'nnGTC
Cuts at: 2626 7016 2626
Size: 4390 3897
AleI CACnn'nnGTG
Cuts at: 1166 2089 5331 1166
Size: 923 3242 4122
Fragments arranged by size:
4122 3242 923
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4008 5186 5535 1184
Size: 2824 1178 349 3936
Fragments arranged by size:
3936 2824 1178 349
AlwNI CAG_nnn'CTG
Cuts at: 2903 3253 3910 4624 6030 7602 2903
Size: 350 657 714 1406 1572 3588
Fragments arranged by size:
3588 1572 1406 714 657 350
ApaI G_GGCC'C
Cuts at: 2041 3530 3598 5437 2041
Size: 1489 68 1839 4891
Fragments arranged by size:
4891 1839 1489 68
ApaLI G'TGCA_C
Cuts at: 2234 4115 5285 5324 6344 7500 2234
Size: 1881 1170 39 1020 1156 3021
Fragments arranged by size:
3021 1881 1170 1156 1020 39
ApoI r'AATT_y
Cuts at: 1493 5596 5822 5974 8083 1493
Size: 4103 226 152 2109 1697
Fragments arranged by size:
4103 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6841 160
Size: 872 5809 1606
Fragments arranged by size:
5809 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8287
AvaI C'yCGr_G
Cuts at: 1870 1943 2124 3056 3585 4725 4875 5612 1870
Size: 73 181 932 529 1140 150 737 4545
Fragments arranged by size:
4545 1140 932 737 529 181 150 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3799 3832 5386 5419 3799
Size: 33 1554 33 6667
Fragments arranged by size:
6667 1554 33 33
BamHI G'GATC_C
Cuts at: 4014 5617 5984 4014
Size: 1603 367 6317
Fragments arranged by size:
6317 1603 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2819 2886 3052 3446 3835
Size: 325 217 627 1032 67 166 394 389
Cuts at: 3835 3918 3924 5343 5608 7063 618
Size: 83 6 1419 265 1455 1842
Fragments arranged by size:
1842 1455 1419 1032 627 394 389 325
265 217 166 83 67 6
BanII G_rGCy'C
Cuts at: 1391 2041 2197 2719 3063 3530 3598 5437 5606
Size: 650 156 522 344 467 68 1839 169
Cuts at: 5606 1391
Size: 4072
Fragments arranged by size:
4072 1839 650 522 467 344 169 156
68
BbeI G_GCGC'C
Cuts at: 2890 5347 2890
Size: 2457 5830
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3398 4289 4499 5254 928
Size: 971 1499 891 210 755 3961
Fragments arranged by size:
3961 1499 971 891 755 210
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2305 2339 5245 5279 5459 5493 6478
Size: 34 368 34 2906 34 180 34 985
Cuts at: 6478 6512 1903
Size: 34 3678
Fragments arranged by size:
3678 2906 985 368 180 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3629 6179 7389 3629
Size: 2550 1210 4527
Fragments arranged by size:
4527 2550 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8287
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2714 3286 3657
Size: 107 122 71 631 605 1042 572 371
Cuts at: 3657 4036 4353 4368 6898 136
Size: 379 317 15 2530 1525
Fragments arranged by size:
2530 1525 1042 631 605 572 379 371
317 122 107 71 15
BglII A'GATC_T
Cuts at: 8282 8282
Size: 8287
BlpI GC'TnA_GC
Cuts at: 2331 5704 2331
Size: 3373 4914
Bme1580I G_kGCm'C
Cuts at: 1790 2041 2238 2822 3057 3216 3451 3530 3598
Size: 251 197 584 235 159 235 79 68
Cuts at: 3598 4119 5289 5328 5437 6348 7504 1790
Size: 521 1170 39 109 911 1156 2573
Fragments arranged by size:
2573 1170 1156 911 584 521 251 235
235 197 159 109 79 68 39
BmgBI CAC'GTC
Cuts at: 1318 3670 3700 5323 1318
Size: 2352 30 1623 4282
Fragments arranged by size:
4282 2352 1623 30
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2719 3533 3848
Size: 416 53 83 399 24 1302 814 315
Cuts at: 3848 5008 6018 6976 442
Size: 1160 1010 958 1753
Fragments arranged by size:
1753 1302 1160 1010 958 814 416 399
315 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2432 5297 1056
Size: 1376 2865 4046
Fragments arranged by size:
4046 2865 1376
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3817 5413 3817
Size: 1596 6691
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3440 3674 4052 4149 4322 4424 4553 4910
Size: 2201 234 378 97 173 102 129 357
Cuts at: 4910 5408 6947 1239
Size: 498 1539 2579
Fragments arranged by size:
2579 2201 1539 498 378 357 234 173
129 102 97
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2653 3713 4472 6412 7277 7575 2653
Size: 1060 759 1940 865 298 3365
Fragments arranged by size:
3365 1940 1060 865 759 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3017 3074 6950 882
Size: 617 1518 57 3876 2219
Fragments arranged by size:
3876 2219 1518 617 57
BsaAI yAC'GTr
Cuts at: 493 1204 2233 6078 493
Size: 711 1029 3845 2702
Fragments arranged by size:
3845 2702 1029 711
BsaBI GATnn'nnATC
Cuts at: 8281 8281
Size: 8287
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2467 2887 5344 6094
Size: 53 83 186 936 934 420 2457 750
Cuts at: 6094 6476 7111 275
Size: 382 635 1451
Fragments arranged by size:
2457 1451 936 934 750 635 420 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 2470 6720 7118 7392 7539 7950 2470
Size: 4250 398 274 147 411 2807
Fragments arranged by size:
4250 2807 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4295 4325 4496 4526 4856
Size: 30 165 30 2892 30 171 30 330
Cuts at: 4856 4886 1178
Size: 30 4579
Fragments arranged by size:
4579 2892 330 171 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2588 2597 3245 4859 5048 5051 5054 5266
Size: 1402 9 648 1614 189 3 3 212
Cuts at: 5266 5516 1186
Size: 250 3957
Fragments arranged by size:
3957 1614 1402 648 250 212 189 9
3 3
BseYI C'CCAG_C
Cuts at: 2040 2637 3016 3027 3300 3408 3647 4839 4913
Size: 597 379 11 273 108 239 1192 74
Cuts at: 4913 5489 7490 7986 2040
Size: 576 2001 496 2341
Fragments arranged by size:
2341 2001 1192 597 576 496 379 273
239 108 74 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2255 3592 3896 3968 4076 4241 4676
Size: 573 519 1337 304 72 108 165 435
Cuts at: 4676 4981 5159 5463 1163
Size: 305 178 304 3987
Fragments arranged by size:
3987 1337 573 519 435 305 304 304
178 165 108 72
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5737 6498 7526 1540
Size: 275 186 3736 761 1028 2301
Fragments arranged by size:
3736 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2238 3771 3867 4119 5289 5328 5508 6348 6433
Size: 1533 96 252 1170 39 180 840 85
Cuts at: 6433 7504 2238
Size: 1071 3021
Fragments arranged by size:
3021 1533 1170 1071 840 252 180 96
85 39
BsmI GAATG_Cn'
Cuts at: 3950 5798 5891 3950
Size: 1848 93 6346
Fragments arranged by size:
6346 1848 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3245 3245
Size: 8287
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2041 2197 2238 2719 2822 3057 3063
Size: 399 251 156 41 481 103 235 6
Cuts at: 3063 3216 3451 3530 3598 3771 3867 4119 5289
Size: 153 235 79 68 173 96 252 1170
Cuts at: 5289 5328 5437 5508 5606 6348 6433 7504 1391
Size: 39 109 71 98 742 85 1071 2174
Fragments arranged by size:
2174 1170 1071 742 481 399 252 251
235 235 173 156 153 109 103 98
96 85 79 71 68 41 39 6
BspEI T'CCGG_A
Cuts at: 2470 7950 2470
Size: 5480 2807
BspHI T'CATG_A
Cuts at: 3771 6176 7906 3771
Size: 2405 1730 4152
Fragments arranged by size:
4152 2405 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3918 4908 5385 5642 844
Size: 3074 990 477 257 3489
Fragments arranged by size:
3489 3074 990 477 257
BsrBI CCG'CTC
Cuts at: 1394 1823 2335 2553 3694 5455 6174 1394
Size: 429 512 218 1141 1761 719 3507
Fragments arranged by size:
3507 1761 1141 719 512 429 218
BsrDI GCAATG_nn'
Cuts at: 65 1248 3463 4200 6782 6956 65
Size: 1183 2215 737 2582 174 1396
Fragments arranged by size:
2582 2215 1396 1183 737 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3280 3915 3921 4398 4835 5066 6931
Size: 110 1470 635 6 477 437 231 1865
Cuts at: 6931 7118 1700
Size: 187 2869
Fragments arranged by size:
2869 1865 1470 635 477 437 231 187
110 6
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8287
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8287
BssSI C'ACGA_G
Cuts at: 4088 6347 7359 4088
Size: 2259 1012 5016
Fragments arranged by size:
5016 2259 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8287
BstBI TT'CG_AA
Cuts at: 7091 7091
Size: 8287
BstEII G'GTnAC_C
Cuts at: 1184 5107 1184
Size: 3923 4364
BstXI CCAn_nnnn'nTGG
Cuts at: 2293 4522 4669 2293
Size: 2229 147 5911
Fragments arranged by size:
5911 2229 147
BstYI r'GATC_y
Cuts at: 1972 4014 4434 5617 5984 6369 6386 7838 7924
Size: 2042 420 1183 367 385 17 1452 86
Cuts at: 7924 7936 8282 1972
Size: 12 346 1977
Fragments arranged by size:
2042 1977 1452 1183 420 385 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 4930 513
Size: 552 3865 3870
Fragments arranged by size:
3870 3865 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7548
Fragments arranged by size:
7548 683 56
BtsI GCAGTG_nn'
Cuts at: 2267 4551 4810 5803 6597 6617 2267
Size: 2284 259 993 794 20 3937
Fragments arranged by size:
3937 2284 993 794 259 20
ClaI AT'CG_AT
Cuts at: 1554 2325 3990 1554
Size: 771 1665 5851
Fragments arranged by size:
5851 1665 771
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4183 4218 529
Size: 35 3619 35 4598
Fragments arranged by size:
4598 3619 35 35
DraI TTT'AAA
Cuts at: 5591 5591
Size: 8287
DraIII CAC_nnn'GTG
Cuts at: 3358 3358
Size: 8287
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2225 2833 4291 7294 809
Size: 507 909 608 1458 3003 1802
Fragments arranged by size:
3003 1802 1458 909 608 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2751 3885 4191
Size: 54 1425 50 275 18 921 1134 306
Cuts at: 4191 4653 5064 5734 6623 8252 8
Size: 462 411 670 889 1629 43
Fragments arranged by size:
1629 1425 1134 921 889 670 462 411
306 275 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5734 1537
Size: 275 3922 4090
Fragments arranged by size:
4090 3922 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2185 2565 2583 2936 3608 6217 1353
Size: 832 380 18 353 672 2609 3423
Fragments arranged by size:
3423 2609 832 672 380 353 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4999 6874 7248 7394 387
Size: 1064 138 3410 1875 374 146 1280
Fragments arranged by size:
3410 1875 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2960 3440 3674 4052 4149 4301 4322
Size: 393 1328 480 234 378 97 152 21
Cuts at: 4322 4424 4553 4720 4910 5408 5438 6350 6947
Size: 102 129 167 190 498 30 912 597
Cuts at: 6947 7734 8002 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1328 912 787 597 498 480 393
378 268 234 190 167 152 129 102
97 30 21
EcoNI CCTnn'n_nnAGG
Cuts at: 2106 3195 3728 4070 4514 5087 2106
Size: 1089 533 342 444 573 5306
Fragments arranged by size:
5306 1089 573 533 444 342
EcoO109I rG'GnC_Cy
Cuts at: 2137 2387 2534 2542 3176 3295 3527 3594 3595
Size: 250 147 8 634 119 232 67 1
Cuts at: 3595 3733 4075 4729 5434 5546 2137
Size: 138 342 654 705 112 4878
Fragments arranged by size:
4878 705 654 634 342 250 232 147
138 119 112 67 8 1
EcoRI G'AATT_C
Cuts at: 5596 5974 5596
Size: 378 7909
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4204 4204
Size: 8287
FseI GG_CCGG'CC
Cuts at: 5070 5070
Size: 8287
FspI TGC'GCA
Cuts at: 3949 4178 4442 5465 5570 6793 3949
Size: 229 264 1023 105 1223 5443
Fragments arranged by size:
5443 1223 1023 264 229 105
FspAI rTGC'GCAy
Cuts at: 3949 3949
Size: 8287
HaeII r_GCGC'y
Cuts at: 1678 2890 3034 3427 3799 4066 4342 4624 5347
Size: 1212 144 393 372 267 276 282 723
Cuts at: 5347 7434 1678
Size: 2087 2531
Fragments arranged by size:
2531 2087 1212 723 393 372 282 276
267 144
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2103 2107 2135 2139 2612
Size: 32 135 32 531 4 28 4 473
Cuts at: 2612 2644 3267 3299 4496 4528 4856 4888 6928
Size: 32 623 32 1197 32 328 32 2040
Cuts at: 6928 6960 7002 7034 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2040 1197 623 531 473 328 135
42 32 32 32 32 32 32 32
32 28 4 4
HincII GTy'rAC
Cuts at: 669 1657 1938 2098 5184 5631 5877 669
Size: 988 281 160 3086 447 246 3079
Fragments arranged by size:
3086 3079 988 447 281 246 160
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8287
HpaI GTT'AAC
Cuts at: 5877 5877
Size: 8287
KasI G'GCGC_C
Cuts at: 2886 5343 2886
Size: 2457 5830
KpnI G_GTAC'C
Cuts at: 5612 5612
Size: 8287
MfeI C'AATT_G
Cuts at: 5886 5886
Size: 8287
MluI A'CGCG_T
Cuts at: 8163 8163
Size: 8287
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3201 3546 4146 7401 7585 1305
Size: 1896 345 600 3255 184 2007
Fragments arranged by size:
3255 2007 1896 600 345 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2753 3887 4193 4655 10
Size: 54 1425 343 921 1134 306 462 3642
Fragments arranged by size:
3642 1425 1134 921 462 343 306 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2089 2241 3050 3833 3990 4130 5090
Size: 648 923 152 809 783 157 140 960
Cuts at: 5090 5331 6245 6604 6763 518
Size: 241 914 359 159 2042
Fragments arranged by size:
2042 960 923 914 809 783 648 359
241 159 157 152 140
MspA1I CmG'CkG
Cuts at: 1606 2348 2362 2460 2506 3412 3453 3508 3742
Size: 742 14 98 46 906 41 55 234
Cuts at: 3742 4359 4594 4800 5515 5579 5703 6380 7528
Size: 617 235 206 715 64 124 677 1148
Cuts at: 7528 7773 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 906 742 715 677 617 245
235 234 206 124 98 64 55 46
41 14
NaeI GCC'GGC
Cuts at: 4400 4837 5068 4400
Size: 437 231 7619
Fragments arranged by size:
7619 437 231
NarI GG'CG_CC
Cuts at: 2887 5344 2887
Size: 2457 5830
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7735
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8287
NgoMIV G'CCGG_C
Cuts at: 4398 4835 5066 4398
Size: 437 231 7619
Fragments arranged by size:
7619 437 231
NheI G'CTAG_C
Cuts at: 1052 2428 5293 1052
Size: 1376 2865 4046
Fragments arranged by size:
4046 2865 1376
NotI GC'GGCC_GC
Cuts at: 5734 5734
Size: 8287
NspI r_CATG'y
Cuts at: 1132 1229 2213 4087 4241 4486 5645 7190 1132
Size: 97 984 1874 154 245 1159 1545 2229
Fragments arranged by size:
2229 1874 1545 1159 984 245 154 97
PasI CC'CwG_GG
Cuts at: 1348 4078 4612 5437 1348
Size: 2730 534 825 4198
Fragments arranged by size:
4198 2730 825 534
PciI A'CATG_T
Cuts at: 7186 7186
Size: 8287
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2815 3523 1108
Size: 1707 708 5872
Fragments arranged by size:
5872 1707 708
PfoI T'CCnGG_A
Cuts at: 4068 4569 5268 4068
Size: 501 699 7087
Fragments arranged by size:
7087 699 501
PmeI GTTT'AAAC
Cuts at: 5591 5591
Size: 8287
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6356 6388 7869 7901 6356
Size: 32 1481 32 6742
Fragments arranged by size:
6742 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2137 2387 2534 4729 5546 2137
Size: 250 147 2195 817 4878
Fragments arranged by size:
4878 2195 817 250 147
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8287
PsiI TTA'TAA
Cuts at: 5857 5857
Size: 8287
PspOMI G'GGCC_C
Cuts at: 2037 3526 3594 5433 2037
Size: 1489 68 1839 4891
Fragments arranged by size:
4891 1839 1489 68
PstI C_TGCA'G
Cuts at: 830 2347 2353 3141 3639 3741 5639 830
Size: 1517 6 788 498 102 1898 3478
Fragments arranged by size:
3478 1898 1517 788 498 102 6
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8287
PvuII CAG'CTG
Cuts at: 2348 2362 2460 2506 3412 5515 5579 2348
Size: 14 98 46 906 2103 64 5056
Fragments arranged by size:
5056 2103 906 98 64 46 14
SalI G'TCGA_C
Cuts at: 1936 5182 5629 1936
Size: 3246 447 4594
Fragments arranged by size:
4594 3246 447
SanDI GG'GwC_CC
Cuts at: 4729 4729
Size: 8287
SapI GCTCTTCn'nnn_
Cuts at: 2185 2583 2936 3608 2185
Size: 398 353 672 6864
Fragments arranged by size:
6864 672 398 353
SbfI CC_TGCA'GG
Cuts at: 5639 5639
Size: 8287
ScaI AGT'ACT
Cuts at: 6535 8247 6535
Size: 1712 6575
SexAI A'CCwGG_T
Cuts at: 4006 4458 4006
Size: 452 7835
SfcI C'TryA_G
Cuts at: 826 1045 2343 2349 3137 3635 3737 4748 5635
Size: 219 1298 6 788 498 102 1011 887
Cuts at: 5635 6770 7451 7642 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1298 1135 1011 887 788 681 498
219 191 102 6
SfiI GGCCn_nnn'nGGCC
Cuts at: 4353 4353
Size: 8287
SfoI GGC'GCC
Cuts at: 2888 5345 2888
Size: 2457 5830
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8287
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8287
SmaI CCC'GGG
Cuts at: 1872 4727 4877 5614 1872
Size: 2855 150 737 4545
Fragments arranged by size:
4545 2855 737 150
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2124 2668 3728 4451 6391 7292
Size: 197 926 181 544 1060 723 1940 901
Cuts at: 7292 7554 820
Size: 262 1553
Fragments arranged by size:
1940 1553 1060 926 901 723 544 262
197 181
SnaBI TAC'GTA
Cuts at: 493 6078 493
Size: 5585 2702
SpeI A'CTAG_T
Cuts at: 152 2224 152
Size: 2072 6215
SphI G_CATG'C
Cuts at: 1132 4087 5645 1132
Size: 2955 1558 3774
Fragments arranged by size:
3774 2955 1558
SspI AAT'ATT
Cuts at: 5 52 6211 5
Size: 47 6159 2081
Fragments arranged by size:
6159 2081 47
StuI AGG'CCT
Cuts at: 1517 4970 1517
Size: 3453 4834
StyI C'CwwG_G
Cuts at: 513 1065 2754 2922 3159 4287 4515 4656 5726
Size: 552 1689 168 237 1128 228 141 1070
Cuts at: 5726 513
Size: 3074
Fragments arranged by size:
3074 1689 1128 1070 552 237 228 168
141
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2101 2263 3008 3449 3485 4023 4710
Size: 1255 521 162 745 441 36 538 687
Cuts at: 4710 4927 5327 6314 6331 6484 6669 7114 325
Size: 217 400 987 17 153 185 445 1498
Fragments arranged by size:
1498 1255 987 745 687 538 521 445
441 400 217 185 162 153 36 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5025 6533 8245
Size: 274 80 33 51 1443 3048 1508 1712
Cuts at: 8245 96
Size: 138
Fragments arranged by size:
3048 1712 1508 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4295 4327 8247 8279 4295
Size: 32 3920 32 4303
Fragments arranged by size:
4303 3920 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8287
XbaI T'CTAG_A
Cuts at: 5623 5623
Size: 8287
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2746 2995 2011
Size: 735 249 7303
Fragments arranged by size:
7303 735 249
XhoI C'TCGA_G
Cuts at: 1943 2124 1943
Size: 181 8106
XmaI C'CCGG_G
Cuts at: 1870 4725 4875 5612 1870
Size: 2855 150 737 4545
Fragments arranged by size:
4545 2855 737 150
XmnI GAAnn'nnTTC
Cuts at: 6416 6416
Size: 8287
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6095 276
Size: 53 83 186 936 4561 2468
Fragments arranged by size:
4561 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FalI FseI FspI FspAI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BbvCI BfrBI Bpu10I BsiWI
BstZ17I Bsu36I EcoICRI EcoRV NruI NsiI PacI PmlI
PspXI PsrI RsrII SacI SacII SrfI SwaI