Restriction map for
pFN21AA0852
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# NO CUT ENZYMES ( 87 enzymes) #
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Acc65I AclI AflII AhdI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI BalI BbeI
BcgI BciVI BfrI BglII BlpI
Bpu1102I BsiWI BspDI BspLU11I BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
CelII ClaI CpoI DraI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoT22I FseI HindIII HpaI KasI
KpnI KspI MfeI MluI MluNI
MscI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflMI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SapI SbfI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspI SwaI Van91I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AatII 495 GACGTC GACGT^C
AflIII 650 ACACGT A^C[GA][CT]GT
AgeI 1415 ACCGGT A^CCGGT
AspI 1980 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 771 CCTAGG C^CTAGG
BamHI 33 GGATCC G^GATCC
BbrPI 2206 CACGTG CAC^GTG
BlnI 771 CCTAGG C^CTAGG
BsiEI 1654 CGGTCG CG[GA][CT]^CG
BspHI 216 TCATGA T^CATGA
BsrDI 1138 GCAATG GCAATG(2/0)
BssSI 2527 CACGAG CACGAG(-5/-1)
BstXI 2810 CCAACATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 592 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 74 CGGCCA [CT]^GGCC[GA]
EcoRI 2625 GAATTC G^AATTC
EcoRV 478 GATATC GAT^ATC
HaeII 524 GGCGCT [GA]GCGC^[CT]
HincII 928 GTCAAC GT[CT]^[GA]AC
HindII 928 GTCAAC GT[CT]^[GA]AC
MslI 2250 CACAGCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 585 CCATGG C^CATGG
PflFI 1980 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1415 ACCGGT A^CCGGT
PmaCI 2206 CACGTG CAC^GTG
PmlI 2206 CACGTG CAC^GTG
PpuMI 2888 AGGACCT [GA]G^G[AT]CC[CT]
PstI 2794 CTGCAG CTGCA^G
RcaI 216 TCATGA T^CATGA
SexAI 1067 ACCTGGT A^CC[AT]GGT
SfiI 2442 GGCCCAGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 2176 CTCAAG C^T[CT][GA]AG
StuI 2735 AGGCCT AGG^CCT
Tth111I 1980 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 37 enzymes) #
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AccI 93 GTCTAC GT^[AC][GT]AC
1637 GTAGAC
AccIII 2382 TCCGGA T^CCGGA
2558 TCCGGA
AcyI 495 GACGTC G[GA]^CG[CT]C
2875 GACGCC
AocI 901 CCTAAGG CC^T[ACGT]AGG
1825 CCTGAGG
ApaI 1005 GGGCCC GGGCC^C
2433 GGGCCC
BanI 1124 GGCACC G^G[CT][GA]CC
1830 GGCACC
BbvCI 1642 CCTCAGC CCTCAGC(-5/-2)
2802 GCTGAGG
BglI 516 GCCAGAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2443 GCCCAGCAGGC
BsaBI 570 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
576 GATCTTCATC
BsaHI 495 GACGTC G[GA]^CG[CT]C
2875 GACGCC
BseAI 2382 TCCGGA T^CCGGA
2558 TCCGGA
BsmBI 493 GAGACG CGTCTC(1/5)
2779 GAGACG
BsmI 264 GAATGC GAATGC(1/-1)
2482 GAATGC
Bsp1407I 626 TGTACA T^GTACA
641 TGTACA
BspEI 2382 TCCGGA T^CCGGA
2558 TCCGGA
BsrBI 1566 CCGCTC CCGCTC(-3/-3)
520 GAGCGG
BsrFI 1121 ACCGGC [GA]^CCGG[CT]
1415 ACCGGT
BsrGI 626 TGTACA T^GTACA
641 TGTACA
Bsu36I 901 CCTAAGG CC^T[ACGT]AGG
1825 CCTGAGG
Cfr10I 1121 ACCGGC [GA]^CCGG[CT]
1415 ACCGGT
DrdI 1640 GACCTCAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2539 GACCTGCTTGTC
DsaI 585 CCATGG C^C[GA][CT]GG
2255 CCGTGG
Eco81I 901 CCTAAGG CC^T[ACGT]AGG
1825 CCTGAGG
EcoNI 2759 CCTCCGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2826 CCTGCAAAAGG
Hin1I 495 GACGTC G[GA]^CG[CT]C
2875 GACGCC
MamI 570 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
576 GATCTTCATC
MroI 2382 TCCGGA T^CCGGA
2558 TCCGGA
MseI 124 TTAA T^TAA
893 TTAA
SacI 2137 GAGCTC GAGCT^C
2183 GAGCTC
ScaI 335 AGTACT AGT^ACT
2660 AGTACT
SfcI 972 CTGTAG C^T[GA][CT]AG
2794 CTGCAG
SspBI 626 TGTACA T^GTACA
641 TGTACA
TaqI 2131 TCGA T^CGA
2537 TCGA
TfiI 384 GATTC G^A[AT]TC
1193 GAATC
Tru9I 124 TTAA T^TAA
893 TTAA
TthHB8I 2131 TCGA T^CGA
2537 TCGA
XcmI 250 CCACTGCCCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2323 CCAAGAGACCGCTGG
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# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII AviII BanII BbsI BbvI
BclI BcnI BfaI BmyI BpmI
BpuAI BsaAI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII EarI EcoO109I
EcoRII EcoT14I FauI FbaI Fnu4HI
FokI FspI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SmaI StyI TseI Tsp45I
Tsp509I TspRI XhoII XmaCI XmaI