(Circular) (Six-base) MAPSORT of: pFN21AA0852.seq Check: 1826 from: 1 to: 7615 pFN21AA0852 7615 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3141 3141 Size: 7615 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2504 5425 278 Size: 53 83 186 936 968 2921 2468 Fragments arranged by size: 2921 2468 968 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2099 3643 4958 1937 Size: 162 1544 1315 4594 Fragments arranged by size: 4594 1544 1315 162 Acc65I G'GTAC_C Cuts at: 4936 4936 Size: 7615 AclI AA'CG_TT Cuts at: 1563 5742 6115 1563 Size: 4179 373 3063 Fragments arranged by size: 4179 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2745 3536 3608 4679 5678 7062 7330 1632 Size: 1113 791 72 1071 999 1384 268 1917 Fragments arranged by size: 1917 1384 1113 1071 999 791 268 72 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7418 AflIII A'CryG_T Cuts at: 1203 2655 6514 7491 1203 Size: 1452 3859 977 1327 Fragments arranged by size: 3859 1452 1327 977 AgeI A'CCGG_T Cuts at: 3420 6446 3420 Size: 3026 4589 AhdI GACnn_n'nnGTC Cuts at: 6344 6344 Size: 7615 AleI CACnn'nnGTG Cuts at: 1166 4259 1166 Size: 3093 4522 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4638 1184 Size: 3454 4161 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2948 2980 2948 Size: 32 7583 AlwNI CAG_nnn'CTG Cuts at: 3901 4046 4807 5358 6930 3901 Size: 145 761 551 1572 4586 Fragments arranged by size: 4586 1572 761 551 145 ApaI G_GGCC'C Cuts at: 3014 4442 3014 Size: 1428 6187 ApaLI G'TGCA_C Cuts at: 5672 6828 5672 Size: 1156 6459 ApoI r'AATT_y Cuts at: 1493 2309 2917 3519 4630 4924 5150 5302 7411 Size: 816 608 602 1111 294 226 152 2109 Cuts at: 7411 1493 Size: 1697 Fragments arranged by size: 2109 1697 1111 816 608 602 294 226 152 AseI AT'TA_AT Cuts at: 160 1032 6169 160 Size: 872 5137 1606 Fragments arranged by size: 5137 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7615 AvaI C'yCGr_G Cuts at: 1870 1943 2280 3792 3936 4731 4940 1870 Size: 73 337 1512 144 795 209 4545 Fragments arranged by size: 4545 1512 795 337 209 144 73 AvrII C'CTAG_G Cuts at: 2776 2776 Size: 7615 BamHI G'GATC_C Cuts at: 2038 4945 5312 2038 Size: 2907 367 4341 Fragments arranged by size: 4341 2907 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3129 3835 4936 6391 618 Size: 325 217 627 1342 706 1101 1455 1842 Fragments arranged by size: 1842 1455 1342 1101 706 627 325 217 BanII G_rGCy'C Cuts at: 1391 2141 3014 3935 4146 4192 4442 4476 4692 Size: 750 873 921 211 46 250 34 216 Cuts at: 4692 4934 1391 Size: 242 4072 Fragments arranged by size: 4072 921 873 750 250 242 216 211 46 34 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2190 3488 3989 928 Size: 971 291 1298 501 4554 Fragments arranged by size: 4554 1298 971 501 291 BbvCI CC'TCA_GC Cuts at: 3648 4808 3648 Size: 1160 6455 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5806 5840 1903 Size: 34 50 34 3785 34 3678 Fragments arranged by size: 3785 3678 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5507 6717 5507 Size: 1210 6405 BclI T'GATC_A Cuts at: 1548 2415 2988 3681 1548 Size: 867 573 693 5482 Fragments arranged by size: 5482 867 693 573 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2527 4454 6226 Size: 107 122 71 631 605 855 1927 1772 Cuts at: 6226 136 Size: 1525 Fragments arranged by size: 1927 1772 1525 855 631 605 122 107 71 BglII A'GATC_T Cuts at: 7610 7610 Size: 7615 BlpI GC'TnA_GC Cuts at: 5032 5032 Size: 7615 Bme1580I G_kGCm'C Cuts at: 1790 2516 2598 3014 3069 4314 4442 5676 6832 Size: 726 82 416 55 1245 128 1234 1156 Cuts at: 6832 1790 Size: 2573 Fragments arranged by size: 2573 1245 1234 1156 726 416 128 82 55 BmgBI CAC'GTC Cuts at: 1318 2196 2658 1318 Size: 878 462 6275 Fragments arranged by size: 6275 878 462 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2371 5346 6304 Size: 416 53 83 399 24 954 2975 958 Cuts at: 6304 442 Size: 1753 Fragments arranged by size: 2975 1753 958 954 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7615 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2548 2772 2548 Size: 224 7391 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2785 3101 3563 3806 3954 6275 1239 Size: 1546 316 462 243 148 2321 2579 Fragments arranged by size: 2579 2321 1546 462 316 243 148 Bpu10I CC'TnA_GC Cuts at: 3648 4520 4808 3648 Size: 872 288 6455 Fragments arranged by size: 6455 872 288 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4166 5740 6605 6903 4166 Size: 1574 865 298 4878 Fragments arranged by size: 4878 1574 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2778 4326 4529 6278 882 Size: 617 1279 1548 203 1749 2219 Fragments arranged by size: 2219 1749 1548 1279 617 203 BsaAI yAC'GTr Cuts at: 493 1204 2710 4213 4309 5406 493 Size: 711 1506 1503 96 1097 2702 Fragments arranged by size: 2702 1506 1503 1097 711 96 BsaBI GATnn'nnATC Cuts at: 2579 2585 7609 2579 Size: 6 5024 2585 Fragments arranged by size: 5024 2585 6 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2501 4881 5422 5804 Size: 53 83 186 936 968 2380 541 382 Cuts at: 5804 6439 275 Size: 635 1451 Fragments arranged by size: 2380 1451 968 936 635 541 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3420 4387 4563 6048 6446 6720 6867 7278 3420 Size: 967 176 1485 398 274 147 411 3757 Fragments arranged by size: 3757 1485 967 411 398 274 176 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7390 Fragments arranged by size: 7390 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2375 3812 4079 4093 4442 4445 4805 1186 Size: 1189 1437 267 14 349 3 360 3996 Fragments arranged by size: 3996 1437 1189 360 349 267 14 3 BseYI C'CCAG_C Cuts at: 3695 4157 4338 4449 6818 7314 3695 Size: 462 181 111 2369 496 3996 Fragments arranged by size: 3996 2369 496 462 181 111 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2624 3914 4193 4862 1163 Size: 573 888 1290 279 669 3916 Fragments arranged by size: 3916 1290 888 669 573 279 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3662 5065 5826 6854 1540 Size: 275 186 1661 1403 761 1028 2301 Fragments arranged by size: 2301 1661 1403 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2559 2709 2896 3156 4146 4192 4526 5676 5761 Size: 150 187 260 990 46 334 1150 85 Cuts at: 5761 6832 2559 Size: 1071 3342 Fragments arranged by size: 3342 1150 1071 990 334 260 187 150 85 46 BsmI GAATG_Cn' Cuts at: 2275 4493 5126 5219 2275 Size: 2218 633 93 4671 Fragments arranged by size: 4671 2218 633 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2492 4778 2492 Size: 2286 5329 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2141 2516 2559 2598 2709 2896 3014 Size: 399 351 375 43 39 111 187 118 Cuts at: 3014 3069 3156 3935 4146 4192 4314 4442 4476 Size: 55 87 779 211 46 122 128 34 Cuts at: 4476 4526 4692 4934 5676 5761 6832 1391 Size: 50 166 242 742 85 1071 2174 Fragments arranged by size: 2174 1071 779 742 399 375 351 242 211 187 166 128 122 118 111 87 85 55 50 46 43 39 34 BspEI T'CCGG_A Cuts at: 4387 4563 7278 4387 Size: 176 2715 4724 Fragments arranged by size: 4724 2715 176 BspHI T'CATG_A Cuts at: 2221 5504 7234 2221 Size: 3283 1730 2602 Fragments arranged by size: 3283 2602 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3141 3682 3889 4554 4970 844 Size: 2297 541 207 665 416 3489 Fragments arranged by size: 3489 2297 665 541 416 207 BsrBI CCG'CTC Cuts at: 1394 1823 2527 3573 5502 1394 Size: 429 704 1046 1929 3507 Fragments arranged by size: 3507 1929 1046 704 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3150 6110 6284 65 Size: 1183 1902 2960 174 1396 Fragments arranged by size: 2960 1902 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3126 3420 6259 6446 1700 Size: 110 1316 294 2839 187 2869 Fragments arranged by size: 2869 2839 1316 294 187 110 BsrGI T'GTAC_A Cuts at: 96 2631 2646 96 Size: 2535 15 5065 Fragments arranged by size: 5065 2535 15 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7615 BssSI C'ACGA_G Cuts at: 4532 5675 6687 4532 Size: 1143 1012 5460 Fragments arranged by size: 5460 1143 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2602 2720 4282 4840 794 Size: 1808 118 1562 558 3569 Fragments arranged by size: 3569 1808 1562 558 118 BstBI TT'CG_AA Cuts at: 6419 6419 Size: 7615 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7615 BstXI CCAn_nnnn'nTGG Cuts at: 4822 4822 Size: 7615 BstYI r'GATC_y Cuts at: 1972 2038 2580 2950 4557 4945 5312 5697 5714 Size: 66 542 370 1607 388 367 385 17 Cuts at: 5714 7166 7252 7264 7610 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1607 1452 542 388 385 370 367 346 86 66 17 12 Bsu36I CC'TnA_GG Cuts at: 2907 3831 2907 Size: 924 6691 BtgI C'CryG_G Cuts at: 513 1065 2590 4260 513 Size: 552 1525 1670 3868 Fragments arranged by size: 3868 1670 1525 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3169 487 Size: 683 56 1943 4933 Fragments arranged by size: 4933 1943 683 56 BtsI GCAGTG_nn' Cuts at: 2255 4360 4512 5131 5925 5945 2255 Size: 2105 152 619 794 20 3925 Fragments arranged by size: 3925 2105 794 619 152 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7615 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7580 DraI TTT'AAA Cuts at: 4919 4919 Size: 7615 DraIII CAC_nnn'GTG Cuts at: 2602 2602 Size: 7615 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3651 4550 6622 809 Size: 507 2335 899 2072 1802 Fragments arranged by size: 2335 2072 1802 899 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2079 5062 5951 Size: 54 1425 50 275 18 249 2983 889 Cuts at: 5951 7580 8 Size: 1629 43 Fragments arranged by size: 2983 1629 1425 889 275 249 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5062 1537 Size: 275 3250 4090 Fragments arranged by size: 4090 3250 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2207 2721 2861 3486 4158 5545 1353 Size: 854 514 140 625 672 1387 3423 Fragments arranged by size: 3423 1387 854 672 625 514 140 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6202 6576 6722 387 Size: 1064 138 4613 374 146 1280 Fragments arranged by size: 4613 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4144 4190 4144 Size: 46 7569 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2745 2785 3101 3536 3563 3608 3806 Size: 393 1113 40 316 435 27 45 198 Cuts at: 3806 3954 4679 5678 6275 7062 7330 1239 Size: 148 725 999 597 787 268 1524 Fragments arranged by size: 1524 1113 999 787 725 597 435 393 316 268 198 148 45 40 27 EcoNI CCTnn'n_nnAGG Cuts at: 4768 4835 4768 Size: 67 7548 EcoO109I rG'GnC_Cy Cuts at: 3547 4439 4894 3547 Size: 892 455 6268 Fragments arranged by size: 6268 892 455 EcoRI G'AATT_C Cuts at: 4630 4924 5302 4630 Size: 294 378 6943 Fragments arranged by size: 6943 378 294 EcoRV GAT'ATC Cuts at: 2485 2485 Size: 7615 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4684 4684 Size: 7615 FspI TGC'GCA Cuts at: 2147 2846 4760 6121 2147 Size: 699 1914 1361 3641 Fragments arranged by size: 3641 1914 1361 699 FspAI rTGC'GCAy Cuts at: 2147 2147 Size: 7615 HaeII r_GCGC'y Cuts at: 1678 2533 6762 1678 Size: 855 4229 2531 Fragments arranged by size: 4229 2531 855 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2453 2485 4513 4545 4631 Size: 32 135 32 881 32 2028 32 86 Cuts at: 4631 4663 6256 6288 6330 6362 1373 Size: 32 1593 32 42 32 2626 Fragments arranged by size: 2626 2028 1593 881 135 86 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2935 4959 5205 669 Size: 988 281 997 2024 246 3079 Fragments arranged by size: 3079 2024 997 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7615 HpaI GTT'AAC Cuts at: 5205 5205 Size: 7615 KpnI G_GTAC'C Cuts at: 4940 4940 Size: 7615 MfeI C'AATT_G Cuts at: 5214 5214 Size: 7615 MluI A'CGCG_T Cuts at: 7491 7491 Size: 7615 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4004 6729 6913 1305 Size: 2699 2725 184 2007 Fragments arranged by size: 2725 2699 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5793 Fragments arranged by size: 5793 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4259 5573 5932 6091 518 Size: 648 3093 1314 359 159 2042 Fragments arranged by size: 3093 2042 1314 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2403 2552 3019 3352 4338 4618 5031 5708 Size: 797 149 467 333 986 280 413 677 Cuts at: 5708 6856 7101 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 986 797 677 467 413 333 280 245 149 NcoI C'CATG_G Cuts at: 513 1065 2590 513 Size: 552 1525 5538 Fragments arranged by size: 5538 1525 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7615 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7615 NotI GC'GGCC_GC Cuts at: 5062 5062 Size: 7615 NspI r_CATG'y Cuts at: 1132 1229 2965 3039 4280 4973 6518 1132 Size: 97 1736 74 1241 693 1545 2229 Fragments arranged by size: 2229 1736 1545 1241 693 97 74 PasI CC'CwG_GG Cuts at: 1348 3457 1348 Size: 2109 5506 PciI A'CATG_T Cuts at: 6514 6514 Size: 7615 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7615 PfoI T'CCnGG_A Cuts at: 3078 3078 Size: 7615 PmeI GTTT'AAAC Cuts at: 4919 4919 Size: 7615 PmlI CAC'GTG Cuts at: 4213 4213 Size: 7615 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5684 5716 7197 7229 5684 Size: 32 1481 32 6070 Fragments arranged by size: 6070 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4894 4894 Size: 7615 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7615 PsiI TTA'TAA Cuts at: 5185 5185 Size: 7615 PspOMI G'GGCC_C Cuts at: 3010 4438 3010 Size: 1428 6187 PstI C_TGCA'G Cuts at: 830 4803 4967 830 Size: 3973 164 3478 Fragments arranged by size: 3973 3478 164 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7615 PvuII CAG'CTG Cuts at: 3019 3352 4618 3019 Size: 333 1266 6016 Fragments arranged by size: 6016 1266 333 SacI G_AGCT'C Cuts at: 4146 4192 4146 Size: 46 7569 SalI G'TCGA_C Cuts at: 1936 4957 1936 Size: 3021 4594 SbfI CC_TGCA'GG Cuts at: 4967 4967 Size: 7615 ScaI AGT'ACT Cuts at: 2342 4667 5863 7575 2342 Size: 2325 1196 1712 2382 Fragments arranged by size: 2382 2325 1712 1196 SexAI A'CCwGG_T Cuts at: 3072 3072 Size: 7615 SfcI C'TryA_G Cuts at: 826 1045 2977 4799 4963 6098 6779 6970 826 Size: 219 1932 1822 164 1135 681 191 1471 Fragments arranged by size: 1932 1822 1471 1135 681 219 191 164 SfiI GGCCn_nnn'nGGCC Cuts at: 4454 4454 Size: 7615 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7615 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7615 SmaI CCC'GGG Cuts at: 1872 2282 3794 4733 4942 1872 Size: 410 1512 939 209 4545 Fragments arranged by size: 4545 1512 939 410 209 SmlI C'TyrA_G Cuts at: 820 1017 1943 4181 5719 6620 6882 820 Size: 197 926 2238 1538 901 262 1553 Fragments arranged by size: 2238 1553 1538 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5406 493 Size: 4913 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7615 SphI G_CATG'C Cuts at: 1132 4973 1132 Size: 3841 3774 SspI AAT'ATT Cuts at: 5 52 5539 5 Size: 47 5487 2081 Fragments arranged by size: 5487 2081 47 StuI AGG'CCT Cuts at: 1517 4742 1517 Size: 3225 4390 StyI C'CwwG_G Cuts at: 513 1065 2590 2776 3117 3600 4737 4905 5054 Size: 552 1525 186 341 483 1137 168 149 Cuts at: 5054 513 Size: 3074 Fragments arranged by size: 3074 1525 1137 552 483 341 186 168 149 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3580 3648 4009 4330 5642 5659 5812 Size: 1255 2000 68 361 321 1312 17 153 Cuts at: 5812 5997 6442 325 Size: 185 445 1498 Fragments arranged by size: 2000 1498 1312 1255 445 361 321 185 153 68 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2340 2631 2646 Size: 274 80 33 51 1443 363 291 15 Cuts at: 2646 2652 4665 5861 7573 96 Size: 6 2013 1196 1712 138 Fragments arranged by size: 2013 1712 1443 1196 363 291 274 138 80 51 33 15 6 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2397 2429 4018 4050 7575 7607 2397 Size: 32 1589 32 3525 32 2405 Fragments arranged by size: 3525 2405 1589 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3988 1316 Size: 2672 4943 XbaI T'CTAG_A Cuts at: 4951 4951 Size: 7615 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2262 4335 2262 Size: 2073 5542 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7615 XmaI C'CCGG_G Cuts at: 1870 2280 3792 4731 4940 1870 Size: 410 1512 939 209 4545 Fragments arranged by size: 4545 1512 939 410 209 XmnI GAAnn'nnTTC Cuts at: 5744 5744 Size: 7615 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2502 5423 276 Size: 53 83 186 936 968 2921 2468 Fragments arranged by size: 2921 2468 968 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI BaeI BbeI BfrBI BsiWI BstZ17I FseI KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacII SanDI SapI SfoI SrfI SwaI