Restriction map for
pFN21AA0852
(Linear) (Six-base) MAPSORT of: KIAA0852.seq Check: 3665 from: 1 to: 2910
KIAA0852.orf
With 182 enzymes: SgfI *
February 28, 2007 11:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1136 2910
Size: 1136 1774
AatII G_ACGT'C
Cuts at: 0 499 2910
Size: 499 2411
AccI GT'mk_AC
Cuts at: 0 94 1638 2910
Size: 94 1544 1272
Fragments arranged by size:
1544 1272 94
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 740 1531 1603 2674 2910
Size: 740 791 72 1071 236
Fragments arranged by size:
1071 791 740 236 72
AflIII A'CryG_T
Cuts at: 0 650 2910
Size: 650 2260
AgeI A'CCGG_T
Cuts at: 0 1415 2910
Size: 1415 1495
AleI CACnn'nnGTG
Cuts at: 0 2254 2910
Size: 2254 656
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2633 2910
Size: 2633 277
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 943 975 2910
Size: 943 32 1935
Fragments arranged by size:
1935 943 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1896 2041 2802 2910
Size: 1896 145 761 108
Fragments arranged by size:
1896 761 145 108
ApaI G_GGCC'C
Cuts at: 0 1009 2437 2910
Size: 1009 1428 473
Fragments arranged by size:
1428 1009 473
ApoI r'AATT_y
Cuts at: 0 304 912 1514 2625 2910
Size: 304 608 602 1111 285
Fragments arranged by size:
1111 608 602 304 285
AvaI C'yCGr_G
Cuts at: 0 275 1787 1931 2726 2910
Size: 275 1512 144 795 184
Fragments arranged by size:
1512 795 275 184 144
AvrII C'CTAG_G
Cuts at: 0 771 2910
Size: 771 2139
BamHI G'GATC_C
Cuts at: 0 33 2910
Size: 33 2877
BanI G'GyrC_C
Cuts at: 0 1124 1830 2910
Size: 1124 706 1080
Fragments arranged by size:
1124 1080 706
BanII G_rGCy'C
Cuts at: 0 136 1009 1930 2141 2187 2437 2471 2687
Size: 136 873 921 211 46 250 34 216
Cuts at: 2687 2910
Size: 223
Fragments arranged by size:
921 873 250 223 216 211 136 46
34
BbsI GAAGACnn'nnnn_
Cuts at: 0 185 1483 1984 2910
Size: 185 1298 501 926
Fragments arranged by size:
1298 926 501 185
BbvCI CC'TCA_GC
Cuts at: 0 1643 2803 2910
Size: 1643 1160 107
Fragments arranged by size:
1643 1160 107
BclI T'GATC_A
Cuts at: 0 410 983 1676 2910
Size: 410 573 693 1234
Fragments arranged by size:
1234 693 573 410
BglI GCCn_nnn'nGGC
Cuts at: 0 522 2449 2910
Size: 522 1927 461
Fragments arranged by size:
1927 522 461
Bme1580I G_kGCm'C
Cuts at: 0 511 593 1009 1064 2309 2437 2910
Size: 511 82 416 55 1245 128 473
Fragments arranged by size:
1245 511 473 416 128 82 55
BmgBI CAC'GTC
Cuts at: 0 191 653 2910
Size: 191 462 2257
Fragments arranged by size:
2257 462 191
BmrI ACTGGGnnnn_n'
Cuts at: 0 366 2910
Size: 366 2544
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 543 767 2910
Size: 543 224 2143
Fragments arranged by size:
2143 543 224
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 780 1096 1558 1801 1949 2910
Size: 780 316 462 243 148 961
Fragments arranged by size:
961 780 462 316 243 148
Bpu10I CC'TnA_GC
Cuts at: 0 1643 2515 2803 2910
Size: 1643 872 288 107
Fragments arranged by size:
1643 872 288 107
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2161 2910
Size: 2161 749
BsaI GGTCTCn'nnnn_
Cuts at: 0 773 2321 2524 2910
Size: 773 1548 203 386
Fragments arranged by size:
1548 773 386 203
BsaAI yAC'GTr
Cuts at: 0 705 2208 2304 2910
Size: 705 1503 96 606
Fragments arranged by size:
1503 705 606 96
BsaBI GATnn'nnATC
Cuts at: 0 574 580 2910
Size: 574 6 2330
Fragments arranged by size:
2330 574 6
BsaHI Gr'CG_yC
Cuts at: 0 496 2876 2910
Size: 496 2380 34
Fragments arranged by size:
2380 496 34
BsaWI w'CCGG_w
Cuts at: 0 1415 2382 2558 2910
Size: 1415 967 176 352
Fragments arranged by size:
1415 967 352 176
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 370 1807 2074 2088 2437 2440 2800 2910
Size: 370 1437 267 14 349 3 360 110
Fragments arranged by size:
1437 370 360 349 267 110 14 3
BseYI C'CCAG_C
Cuts at: 0 1690 2152 2333 2444 2910
Size: 1690 462 181 111 466
Fragments arranged by size:
1690 466 462 181 111
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 619 1909 2188 2857 2910
Size: 619 1290 279 669 53
Fragments arranged by size:
1290 669 619 279 53
BsiEI CG_ry'CG
Cuts at: 0 1657 2910
Size: 1657 1253
BsiHKAI G_wGCw'C
Cuts at: 0 554 704 891 1151 2141 2187 2521 2910
Size: 554 150 187 260 990 46 334 389
Fragments arranged by size:
990 554 389 334 260 187 150 46
BsmI GAATG_Cn'
Cuts at: 0 270 2488 2910
Size: 270 2218 422
Fragments arranged by size:
2218 422 270
BsmBI CGTCTCn'nnnn_
Cuts at: 0 487 2773 2910
Size: 487 2286 137
Fragments arranged by size:
2286 487 137
Bsp1286I G_dGCh'C
Cuts at: 0 136 511 554 593 704 891 1009 1064
Size: 136 375 43 39 111 187 118 55
Cuts at: 1064 1151 1930 2141 2187 2309 2437 2471 2521
Size: 87 779 211 46 122 128 34 50
Cuts at: 2521 2687 2910
Size: 166 223
Fragments arranged by size:
779 375 223 211 187 166 136 128
122 118 111 87 55 50 46 43
39 34
BspEI T'CCGG_A
Cuts at: 0 2382 2558 2910
Size: 2382 176 352
Fragments arranged by size:
2382 352 176
BspHI T'CATG_A
Cuts at: 0 216 2910
Size: 216 2694
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1136 1677 1884 2549 2910
Size: 1136 541 207 665 361
Fragments arranged by size:
1136 665 541 361 207
BsrBI CCG'CTC
Cuts at: 0 522 1568 2910
Size: 522 1046 1342
Fragments arranged by size:
1342 1046 522
BsrDI GCAATG_nn'
Cuts at: 0 1145 2910
Size: 1145 1765
BsrFI r'CCGG_y
Cuts at: 0 1121 1415 2910
Size: 1121 294 1495
Fragments arranged by size:
1495 1121 294
BsrGI T'GTAC_A
Cuts at: 0 626 641 2910
Size: 626 15 2269
Fragments arranged by size:
2269 626 15
BssSI C'ACGA_G
Cuts at: 0 2527 2910
Size: 2527 383
BstAPI GCAn_nnn'nTGC
Cuts at: 0 597 715 2277 2835 2910
Size: 597 118 1562 558 75
Fragments arranged by size:
1562 597 558 118 75
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2817 2910
Size: 2817 93
BstYI r'GATC_y
Cuts at: 0 33 575 945 2552 2910
Size: 33 542 370 1607 358
Fragments arranged by size:
1607 542 370 358 33
Bsu36I CC'TnA_GG
Cuts at: 0 902 1826 2910
Size: 902 924 1084
Fragments arranged by size:
1084 924 902
BtgI C'CryG_G
Cuts at: 0 585 2255 2910
Size: 585 1670 655
Fragments arranged by size:
1670 655 585
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1164 2910
Size: 1164 1746
BtsI GCAGTG_nn'
Cuts at: 0 250 2355 2507 2910
Size: 250 2105 152 403
Fragments arranged by size:
2105 403 250 152
DraIII CAC_nnn'GTG
Cuts at: 0 597 2910
Size: 597 2313
DrdI GACnn_nn'nnGTC
Cuts at: 0 1646 2545 2910
Size: 1646 899 365
Fragments arranged by size:
1646 899 365
EaeI y'GGCC_r
Cuts at: 0 74 2910
Size: 74 2836
EarI CTCTTCn'nnn_
Cuts at: 0 202 716 856 1481 2153 2910
Size: 202 514 140 625 672 757
Fragments arranged by size:
757 672 625 514 202 140
EcoICRI GAG'CTC
Cuts at: 0 2139 2185 2910
Size: 2139 46 725
Fragments arranged by size:
2139 725 46
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 740 780 1096 1531 1558 1603 1801 1949
Size: 740 40 316 435 27 45 198 148
Cuts at: 1949 2674 2910
Size: 725 236
Fragments arranged by size:
740 725 435 316 236 198 148 45
40 27
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2763 2830 2910
Size: 2763 67 80
Fragments arranged by size:
2763 80 67
EcoO109I rG'GnC_Cy
Cuts at: 0 1542 2434 2889 2910
Size: 1542 892 455 21
Fragments arranged by size:
1542 892 455 21
EcoRI G'AATT_C
Cuts at: 0 2625 2910
Size: 2625 285
EcoRV GAT'ATC
Cuts at: 0 480 2910
Size: 480 2430
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2679 2910
Size: 2679 231
FspI TGC'GCA
Cuts at: 0 142 841 2755 2910
Size: 142 699 1914 155
Fragments arranged by size:
1914 699 155 142
FspAI rTGC'GCAy
Cuts at: 0 142 2910
Size: 142 2768
HaeII r_GCGC'y
Cuts at: 0 528 2910
Size: 528 2382
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 448 480 2508 2540 2626 2658 2910
Size: 448 32 2028 32 86 32 252
Fragments arranged by size:
2028 448 252 86 32 32 32
HincII GTy'rAC
Cuts at: 0 930 2910
Size: 930 1980
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1999 2910
Size: 1999 911
MslI CAynn'nnrTG
Cuts at: 0 2254 2910
Size: 2254 656
MspA1I CmG'CkG
Cuts at: 0 398 547 1014 1347 2333 2613 2910
Size: 398 149 467 333 986 280 297
Fragments arranged by size:
986 467 398 333 297 280 149
NcoI C'CATG_G
Cuts at: 0 585 2910
Size: 585 2325
NspI r_CATG'y
Cuts at: 0 960 1034 2275 2910
Size: 960 74 1241 635
Fragments arranged by size:
1241 960 635 74
PasI CC'CwG_GG
Cuts at: 0 1452 2910
Size: 1452 1458
PfoI T'CCnGG_A
Cuts at: 0 1073 2910
Size: 1073 1837
PmlI CAC'GTG
Cuts at: 0 2208 2910
Size: 2208 702
PpuMI rG'GwC_Cy
Cuts at: 0 2889 2910
Size: 2889 21
PspOMI G'GGCC_C
Cuts at: 0 1005 2433 2910
Size: 1005 1428 477
Fragments arranged by size:
1428 1005 477
PstI C_TGCA'G
Cuts at: 0 2798 2910
Size: 2798 112
PvuII CAG'CTG
Cuts at: 0 1014 1347 2613 2910
Size: 1014 333 1266 297
Fragments arranged by size:
1266 1014 333 297
SacI G_AGCT'C
Cuts at: 0 2141 2187 2910
Size: 2141 46 723
Fragments arranged by size:
2141 723 46
ScaI AGT'ACT
Cuts at: 0 337 2662 2910
Size: 337 2325 248
Fragments arranged by size:
2325 337 248
SexAI A'CCwGG_T
Cuts at: 0 1067 2910
Size: 1067 1843
SfcI C'TryA_G
Cuts at: 0 972 2794 2910
Size: 972 1822 116
Fragments arranged by size:
1822 972 116
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2449 2910
Size: 2449 461
SmaI CCC'GGG
Cuts at: 0 277 1789 2728 2910
Size: 277 1512 939 182
Fragments arranged by size:
1512 939 277 182
SmlI C'TyrA_G
Cuts at: 0 2176 2910
Size: 2176 734
StuI AGG'CCT
Cuts at: 0 2737 2910
Size: 2737 173
StyI C'CwwG_G
Cuts at: 0 585 771 1112 1595 2732 2900 2910
Size: 585 186 341 483 1137 168 10
Fragments arranged by size:
1137 585 483 341 186 168 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1575 1643 2004 2325 2910
Size: 1575 68 361 321 585
Fragments arranged by size:
1575 585 361 321 68
TatI w'GTAC_w
Cuts at: 0 335 626 641 647 2660 2910
Size: 335 291 15 6 2013 250
Fragments arranged by size:
2013 335 291 250 15 6
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 392 424 2013 2045 2910
Size: 392 32 1589 32 865
Fragments arranged by size:
1589 865 392 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1983 2910
Size: 1983 927
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 257 2330 2910
Size: 257 2073 580
Fragments arranged by size:
2073 580 257
XmaI C'CCGG_G
Cuts at: 0 275 1787 2726 2910
Size: 275 1512 939 184
Fragments arranged by size:
1512 939 275 184
ZraI GAC'GTC
Cuts at: 0 497 2910
Size: 497 2413
Enzymes that do cut:
AarI AatII AccI AcuI AflIII AgeI AleI AlfI
AloI AlwNI ApaI ApoI AvaI AvrII BamHI BanI
BanII BbsI BbvCI BclI BglI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraIII DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI FspAI HaeII Hin4I HincII
MmeI MslI MspA1I NcoI NspI PasI PfoI PmlI
PpuMI PspOMI PstI PvuII SacI ScaI SexAI SfcI
SfiI SmaI SmlI StuI StyI TaqII TatI TstI
Tth111I XcmI XmaI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AhdI ApaLI AscI AseI
AsiSI BaeI BbeI BcgI BciVI BfrBI BglII BlpI
BmtI BsaXI BsiWI BssHII BstBI BstEII BstZ17I ClaI
CspCI DraI EagI EciI FseI HindIII HpaI KasI
KpnI MfeI MluI MscI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PciI PflMI PmeI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII
SalI SanDI SapI SbfI SfoI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI SwaI XbaI XhoI XmnI