Restriction map for
pFN21AA0837
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# NO CUT ENZYMES ( 104 enzymes) #
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AatII Acc65I AccI AccII AclI
AflII AgeI AhdI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BamHI BbrPI BbsI
BcgI BfrI BlnI BpuAI BsaBI
BseRI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspHI BsrDI BsrGI
BssHII Bst1107I BstAPI BstEII BstPI
BstUI BstZ17I ClaI CpoI DraIII
DrdI EagI Eam1105I EclXI Eco52I
EcoO65I EcoRI EcoT22I FseI FspI
HgaI HincII HindII HindIII HpaI
KpnI KspI MamI MluI MvnI
NheI NotI NruI NsiI PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SacII SalI SbfI SfcI SfiI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SspI SwaI Tth111I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AcsI 466 GAATTT [GA]^AATT[CT]
AcyI 1518 GGCGCC G[GA]^CG[CT]C
Alw44I 1461 GTGCAC G^TGCAC
AlwNI 430 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1232 AGCGCT AGC^GCT
ApaLI 1461 GTGCAC G^TGCAC
ApoI 466 GAATTT [GA]^AATT[CT]
AvaI 1783 CCCGAG C^[CT]CG[GA]G
BbeI 1518 GGCGCC GGCGC^C
BbvCI 310 CCTCAGC CCTCAGC(-5/-2)
BciVI 98 GGATAC GTATCC(6/5)
BglI 454 GCCGACAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 372 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 372 GCTTAGC GC^T[ACGT]AGC
BsaAI 1510 CACGTA [CT]AC^GT[GA]
BsaHI 1518 GGCGCC G[GA]^CG[CT]C
BsaI 2106 GAGACC GGTCTC(1/5)
BsgI 1947 CTGCAC GTGCAG(16/14)
BsoBI 1783 CCCGAG C^[CT]CG[GA]G
BspMI 2028 GCAGGT ACCTGC(4/8)
BsrBI 601 CCGCTC CCGCTC(-3/-3)
BstBI 763 TTCGAA TT^CGAA
BstXI 587 CCATGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 372 GCTTAGC GC^T[ACGT]AGC
DraI 1753 TTTAAA TTT^AAA
Eco47III 1232 AGCGCT AGC^GCT
EcoNI 112 CCTGAGCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1114 GATATC GAT^ATC
Hin1I 1518 GGCGCC G[GA]^CG[CT]C
KasI 1518 GGCGCC G^GCGCC
MfeI 495 CAATTG C^AATTG
MunI 495 CAATTG C^AATTG
NaeI 1710 GCCGGC GCC^GGC
NarI 1518 GGCGCC GG^CGCC
NdeI 1934 CATATG CA^TATG
NgoMIV 1710 GCCGGC G^CCGGC
NspV 763 TTCGAA TT^CGAA
PvuII 1466 CAGCTG CAG^CTG
SapI 2143 GAAGAGC GCTCTTC(1/4)
ScaI 2123 AGTACT AGT^ACT
SexAI 353 ACCAGGT A^CC[AT]GGT
SfoI 1518 GGCGCC GGC^GCC
SfuI 763 TTCGAA TT^CGAA
SmlI 736 CTCAAG C^T[CT][GA]AG
SphI 505 GCATGC GCATG^C
TaqI 764 TCGA T^CGA
TthHB8I 764 TCGA T^CGA
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# TWO CUT ENZYMES ( 25 enzymes) #
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AflIII 1046 ACATGT A^C[GA][CT]GT
2057 ACATGT
AocI 93 CCTGAGG CC^T[ACGT]AGG
1502 CCTCAGG
AspHI 1018 GTGCTC G[AT]GC[AT]^C
1461 GTGCAC
BalI 837 TGGCCA TGG^CCA
1449 TGGCCA
BclI 513 TGATCA T^GATCA
743 TGATCA
BcnI 341 CCCGG CC^[GC]GG
1413 CCGGG
BglII 1202 AGATCT A^GATCT
1941 AGATCT
BsiHKAI 1018 GTGCTC G[AT]GC[AT]^C
1461 GTGCAC
BspLU11I 1046 ACATGT A^CATGT
2057 ACATGT
BsrFI 212 ACCGGC [GA]^CCGG[CT]
1710 GCCGGC
BssSI 16 CTCGTG CACGAG(-5/-1)
163 CTCGTG
Bsu36I 93 CCTGAGG CC^T[ACGT]AGG
1502 CCTCAGG
Cfr10I 212 ACCGGC [GA]^CCGG[CT]
1710 GCCGGC
Eco81I 93 CCTGAGG CC^T[ACGT]AGG
1502 CCTCAGG
FbaI 513 TGATCA T^GATCA
743 TGATCA
HaeII 1232 AGCGCT [GA]GCGC^[CT]
1518 GGCGCC
MaeII 950 ACGT A^CGT
1511 ACGT
MluNI 837 TGGCCA TGG^CCA
1449 TGGCCA
MscI 837 TGGCCA TGG^CCA
1449 TGGCCA
MspA1I 650 CAGCGG C[AC]G^C[GT]G
1466 CAGCTG
NciI 341 CCCGG CC^[GC]GG
1413 CCGGG
PflMI 444 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1815 CCAACCTGTGG
PleI 726 GACTC GAGTC(4/5)
2012 GACTC
Tsp45I 122 GTGAC ^GT[GC]AC
982 GTGAC
Van91I 444 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1815 CCAACCTGTGG
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# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AccIII AciI AfaI AluI AlwI
ApaI AvaII BanI BanII BbvI
BfaI BmyI BpmI BsaJI BsaWI
BseAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspEI BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MroI MseI MslI
MspI MvaI MwoI NcoI NdeII
NlaIII NlaIV NspI RsaI Sau3AI
Sau96I ScrFI SfaNI StuI StyI
TfiI Tru9I TseI Tsp509I TspRI
XcmI XhoII