Restriction map for
pFN21AA0837
(Linear) (Six-base) MAPSORT of: KIAA0837.seq Check: 5865 from: 1 to: 2166
KIAA0837.orf
With 182 enzymes: SgfI *
February 28, 2007 11:21 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1191 1910 1984 2166
Size: 1191 719 74 182
Fragments arranged by size:
1191 719 182 74
AfeI AGC'GCT
Cuts at: 0 1234 2166
Size: 1234 932
AflIII A'CryG_T
Cuts at: 0 1046 2057 2166
Size: 1046 1011 109
Fragments arranged by size:
1046 1011 109
AleI CACnn'nnGTG
Cuts at: 0 163 2166
Size: 163 2003
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1894 1962 2166
Size: 1894 68 204
Fragments arranged by size:
1894 204 68
AlwNI CAG_nnn'CTG
Cuts at: 0 435 2166
Size: 435 1731
ApaI G_GGCC'C
Cuts at: 0 395 622 1911 2166
Size: 395 227 1289 255
Fragments arranged by size:
1289 395 255 227
ApaLI G'TGCA_C
Cuts at: 0 1461 2166
Size: 1461 705
ApoI r'AATT_y
Cuts at: 0 466 2166
Size: 466 1700
AvaI C'yCGr_G
Cuts at: 0 1783 2166
Size: 1783 383
BanI G'GyrC_C
Cuts at: 0 175 1427 1518 2166
Size: 175 1252 91 648
Fragments arranged by size:
1252 648 175 91
BanII G_rGCy'C
Cuts at: 0 31 395 622 1911 2166
Size: 31 364 227 1289 255
Fragments arranged by size:
1289 364 255 227 31
BbeI G_GCGC'C
Cuts at: 0 1522 2166
Size: 1522 644
BbvCI CC'TCA_GC
Cuts at: 0 311 2166
Size: 311 1855
BciVI GTATCCnnnnn_n'
Cuts at: 0 92 2166
Size: 92 2074
BclI T'GATC_A
Cuts at: 0 513 743 2166
Size: 513 230 1423
Fragments arranged by size:
1423 513 230
BglI GCCn_nnn'nGGC
Cuts at: 0 460 2166
Size: 460 1706
BglII A'GATC_T
Cuts at: 0 1202 1941 2166
Size: 1202 739 225
Fragments arranged by size:
1202 739 225
BlpI GC'TnA_GC
Cuts at: 0 373 2166
Size: 373 1793
Bme1580I G_kGCm'C
Cuts at: 0 180 285 395 622 866 1465 1511 1911
Size: 180 105 110 227 244 599 46 400
Cuts at: 1911 2166
Size: 255
Fragments arranged by size:
599 400 255 244 227 180 110 105
46
BmrI ACTGGGnnnn_n'
Cuts at: 0 1581 2166
Size: 1581 585
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 472 713 1264 1394 1709 2166
Size: 88 384 241 551 130 315 457
Fragments arranged by size:
551 457 384 315 241 130 88
Bpu10I CC'TnA_GC
Cuts at: 0 113 311 416 1763 2111 2166
Size: 113 198 105 1347 348 55
Fragments arranged by size:
1347 348 198 113 105 55
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 721 2166
Size: 721 1445
BsaI GGTCTCn'nnnn_
Cuts at: 0 2100 2166
Size: 2100 66
BsaAI yAC'GTr
Cuts at: 0 1512 2166
Size: 1512 654
BsaHI Gr'CG_yC
Cuts at: 0 1519 2166
Size: 1519 647
BsaWI w'CCGG_w
Cuts at: 0 478 1187 1277 1808 2166
Size: 478 709 90 531 358
Fragments arranged by size:
709 531 478 358 90
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1079 1109 1760 1790 2143 2166
Size: 1079 30 651 30 353 23
Fragments arranged by size:
1079 651 353 30 30 23
BseYI C'CCAG_C
Cuts at: 0 1383 2166
Size: 1383 783
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1932 2166
Size: 1932 234
BsiHKAI G_wGCw'C
Cuts at: 0 1022 1465 2166
Size: 1022 443 701
Fragments arranged by size:
1022 701 443
Bsp1286I G_dGCh'C
Cuts at: 0 31 180 285 395 622 866 1022 1465
Size: 31 149 105 110 227 244 156 443
Cuts at: 1465 1511 1911 2166
Size: 46 400 255
Fragments arranged by size:
443 400 255 244 227 156 149 110
105 46 31
BspEI T'CCGG_A
Cuts at: 0 478 1277 1808 2166
Size: 478 799 531 358
Fragments arranged by size:
799 531 478 358
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2019 2166
Size: 2019 147
BsrBI CCG'CTC
Cuts at: 0 603 2166
Size: 603 1563
BsrFI r'CCGG_y
Cuts at: 0 212 1710 2166
Size: 212 1498 456
Fragments arranged by size:
1498 456 212
BssSI C'ACGA_G
Cuts at: 0 16 163 2166
Size: 16 147 2003
Fragments arranged by size:
2003 147 16
BstBI TT'CG_AA
Cuts at: 0 764 2166
Size: 764 1402
BstXI CCAn_nnnn'nTGG
Cuts at: 0 594 2166
Size: 594 1572
BstYI r'GATC_y
Cuts at: 0 89 1202 1638 1941 1959 2166
Size: 89 1113 436 303 18 207
Fragments arranged by size:
1113 436 303 207 89 18
Bsu36I CC'TnA_GG
Cuts at: 0 94 1503 2166
Size: 94 1409 663
Fragments arranged by size:
1409 663 94
BtgI C'CryG_G
Cuts at: 0 366 587 824 942 1083 1839 2166
Size: 366 221 237 118 141 756 327
Fragments arranged by size:
756 366 327 237 221 141 118
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 946 1685 2166
Size: 946 739 481
Fragments arranged by size:
946 739 481
DraI TTT'AAA
Cuts at: 0 1755 2166
Size: 1755 411
EaeI y'GGCC_r
Cuts at: 0 152 214 452 544 837 1449 2166
Size: 152 62 238 92 293 612 717
Fragments arranged by size:
717 612 293 238 152 92 62
EarI CTCTTCn'nnn_
Cuts at: 0 1324 1909 2138 2166
Size: 1324 585 229 28
Fragments arranged by size:
1324 585 229 28
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 352 1108 2166
Size: 352 756 1058
Fragments arranged by size:
1058 756 352
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 472 713 1191 1264 1394 1709 1910
Size: 88 384 241 478 73 130 315 201
Cuts at: 1910 1984 2166
Size: 74 182
Fragments arranged by size:
478 384 315 241 201 182 130 88
74 73
EcoNI CCTnn'n_nnAGG
Cuts at: 0 116 2166
Size: 116 2050
EcoO109I rG'GnC_Cy
Cuts at: 0 392 619 1659 2166
Size: 392 227 1040 507
Fragments arranged by size:
1040 507 392 227
EcoRV GAT'ATC
Cuts at: 0 1116 2166
Size: 1116 1050
HaeII r_GCGC'y
Cuts at: 0 1236 1522 2166
Size: 1236 286 644
Fragments arranged by size:
1236 644 286
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 868 900 1486 1518 2047 2079 2166
Size: 868 32 586 32 529 32 87
Fragments arranged by size:
868 586 529 87 32 32 32
KasI G'GCGC_C
Cuts at: 0 1518 2166
Size: 1518 648
MfeI C'AATT_G
Cuts at: 0 495 2166
Size: 495 1671
MscI TGG'CCA
Cuts at: 0 839 1451 2166
Size: 839 612 715
Fragments arranged by size:
839 715 612
MslI CAynn'nnrTG
Cuts at: 0 163 329 592 751 1876 2166
Size: 163 166 263 159 1125 290
Fragments arranged by size:
1125 290 263 166 163 159
MspA1I CmG'CkG
Cuts at: 0 652 1468 2166
Size: 652 816 698
Fragments arranged by size:
816 698 652
NaeI GCC'GGC
Cuts at: 0 1712 2166
Size: 1712 454
NarI GG'CG_CC
Cuts at: 0 1519 2166
Size: 1519 647
NcoI C'CATG_G
Cuts at: 0 587 942 1839 2166
Size: 587 355 897 327
Fragments arranged by size:
897 587 355 327
NdeI CA'TA_TG
Cuts at: 0 1935 2166
Size: 1935 231
NgoMIV G'CCGG_C
Cuts at: 0 1710 2166
Size: 1710 456
NspI r_CATG'y
Cuts at: 0 509 712 1050 2061 2166
Size: 509 203 338 1011 105
Fragments arranged by size:
1011 509 338 203 105
PasI CC'CwG_GG
Cuts at: 0 615 622 2166
Size: 615 7 1544
Fragments arranged by size:
1544 615 7
PciI A'CATG_T
Cuts at: 0 1046 2057 2166
Size: 1046 1011 109
Fragments arranged by size:
1046 1011 109
PflMI CCAn_nnn'nTGG
Cuts at: 0 450 1821 2166
Size: 450 1371 345
Fragments arranged by size:
1371 450 345
PfoI T'CCnGG_A
Cuts at: 0 1241 2166
Size: 1241 925
PspOMI G'GGCC_C
Cuts at: 0 391 618 1907 2166
Size: 391 227 1289 259
Fragments arranged by size:
1289 391 259 227
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 343 375 1177 1209 2166
Size: 343 32 802 32 957
Fragments arranged by size:
957 802 343 32 32
PvuII CAG'CTG
Cuts at: 0 1468 2166
Size: 1468 698
SapI GCTCTTCn'nnn_
Cuts at: 0 2138 2166
Size: 2138 28
ScaI AGT'ACT
Cuts at: 0 2125 2166
Size: 2125 41
SexAI A'CCwGG_T
Cuts at: 0 353 2166
Size: 353 1813
SfoI GGC'GCC
Cuts at: 0 1520 2166
Size: 1520 646
SmlI C'TyrA_G
Cuts at: 0 736 2166
Size: 736 1430
SphI G_CATG'C
Cuts at: 0 509 2166
Size: 509 1657
StuI AGG'CCT
Cuts at: 0 223 734 1858 2166
Size: 223 511 1124 308
Fragments arranged by size:
1124 511 308 223
StyI C'CwwG_G
Cuts at: 0 587 942 1839 2166
Size: 587 355 897 327
Fragments arranged by size:
897 587 355 327
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 163 955 1340 2166
Size: 163 792 385 826
Fragments arranged by size:
826 792 385 163
TatI w'GTAC_w
Cuts at: 0 2123 2166
Size: 2123 43
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 74 106 1601 1633 2166
Size: 74 32 1495 32 533
Fragments arranged by size:
1495 533 74 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 822 1164 1232 2166
Size: 822 342 68 934
Fragments arranged by size:
934 822 342 68
Enzymes that do cut:
AcuI AfeI AflIII AleI AlfI AlwNI ApaI ApaLI
ApoI AvaI BanI BanII BbeI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BpmI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaWI BsaXI BseYI BsgI BsiHKAI
Bsp1286I BspEI BspMI BsrBI BsrFI BssSI BstBI BstXI
BstYI Bsu36I BtgI BtgZI DraI EaeI EarI EciI
Eco57MI EcoNI EcoO109I EcoRV HaeII Hin4I KasI MfeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NspI PasI PciI PflMI PfoI PspOMI PsrI PvuII
SapI ScaI SexAI SfoI SmlI SphI StuI StyI
TaqII TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AhdI
AloI AscI AseI AsiSI AvrII BaeI BamHI BbsI
BcgI BfrBI BmgBI BmtI BplI BsaBI BseRI BsiEI
BsiWI BsmI BsmBI BspHI BsrDI BsrGI BssHII BstAPI
BstEII BstZ17I BtsI ClaI CspCI DraIII DrdI EagI
EcoICRI EcoRI FalI FseI FspI FspAI HincII HindIII
HpaI KpnI MluI MmeI NheI NotI NruI NsiI
PacI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI
PstI PvuI RsrII SacI SacII SalI SanDI SbfI
SfcI SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI
SspI SwaI Tth111I XbaI XhoI XmaI XmnI ZraI