(Circular) (Six-base) MAPSORT of: pFN21AA0835.seq Check: 2288 from: 1 to: 8068 pFN21AA0835 8068 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2601 2601 Size: 8068 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2363 5878 278 Size: 53 83 186 936 827 3515 2468 Fragments arranged by size: 3515 2468 936 827 186 83 53 AccI GT'mk_AC Cuts at: 1937 3993 5411 1937 Size: 2056 1418 4594 Fragments arranged by size: 4594 2056 1418 Acc65I G'GTAC_C Cuts at: 5389 5389 Size: 8068 AclI AA'CG_TT Cuts at: 1563 6195 6568 1563 Size: 4632 373 3063 Fragments arranged by size: 4632 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2261 2418 2550 2582 3185 3476 4299 4382 Size: 629 157 132 32 603 291 823 83 Cuts at: 4382 4841 5141 6131 7515 7783 1632 Size: 459 300 990 1384 268 1917 Fragments arranged by size: 1917 1384 990 823 629 603 459 300 291 268 157 132 83 32 AfeI AGC'GCT Cuts at: 2696 2696 Size: 8068 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7871 AflIII A'CryG_T Cuts at: 1203 4992 5292 6967 7944 1203 Size: 3789 300 1675 977 1327 Fragments arranged by size: 3789 1675 1327 977 300 AgeI A'CCGG_T Cuts at: 6899 6899 Size: 8068 AhdI GACnn_n'nnGTC Cuts at: 6797 6797 Size: 8068 AleI CACnn'nnGTG Cuts at: 1166 2297 1166 Size: 1131 6937 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3815 4768 1184 Size: 2631 953 4484 Fragments arranged by size: 4484 2631 953 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4436 4468 4436 Size: 32 8036 AlwNI CAG_nnn'CTG Cuts at: 2579 2707 2722 2930 3095 4068 5811 7383 2579 Size: 128 15 208 165 973 1743 1572 3264 Fragments arranged by size: 3264 1743 1572 973 208 165 128 15 ApaI G_GGCC'C Cuts at: 2996 2996 Size: 8068 ApaLI G'TGCA_C Cuts at: 2588 6125 7281 2588 Size: 3537 1156 3375 Fragments arranged by size: 3537 3375 1156 ApoI r'AATT_y Cuts at: 1493 4309 5260 5377 5603 5755 7864 1493 Size: 2816 951 117 226 152 2109 1697 Fragments arranged by size: 2816 2109 1697 951 226 152 117 AseI AT'TA_AT Cuts at: 160 1032 6622 160 Size: 872 5590 1606 Fragments arranged by size: 5590 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8068 AvaI C'yCGr_G Cuts at: 1870 1943 2387 3126 3659 4667 4849 5393 1870 Size: 73 444 739 533 1008 182 544 4545 Fragments arranged by size: 4545 1008 739 544 533 444 182 73 BamHI G'GATC_C Cuts at: 2429 3417 3627 5398 5765 2429 Size: 988 210 1771 367 4732 Fragments arranged by size: 4732 1771 988 367 210 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2145 4639 5389 6844 618 Size: 325 217 627 358 2494 750 1455 1842 Fragments arranged by size: 2494 1842 1455 750 627 358 325 217 BanII G_rGCy'C Cuts at: 1391 2035 2411 2988 2996 4707 5387 1391 Size: 644 376 577 8 1711 680 4072 Fragments arranged by size: 4072 1711 680 644 577 376 8 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2028 2955 928 Size: 971 129 927 6041 Fragments arranged by size: 6041 971 927 129 BbvCI CC'TCA_GC Cuts at: 2087 2199 3944 2087 Size: 112 1745 6211 Fragments arranged by size: 6211 1745 112 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5347 5381 6259 6293 1903 Size: 34 3410 34 878 34 3678 Fragments arranged by size: 3678 3410 878 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4338 5960 7170 4338 Size: 1622 1210 5236 Fragments arranged by size: 5236 1622 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 8068 BfrBI ATG'CAT Cuts at: 3442 3442 Size: 8068 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2975 3167 3228 Size: 107 122 71 631 605 1303 192 61 Cuts at: 3228 3396 3722 6679 136 Size: 168 326 2957 1525 Fragments arranged by size: 2957 1525 1303 631 605 326 192 168 122 107 71 61 BglII A'GATC_T Cuts at: 3429 5097 8063 3429 Size: 1668 2966 3434 Fragments arranged by size: 3434 2966 1668 BlpI GC'TnA_GC Cuts at: 2205 5485 2205 Size: 3280 4788 Bme1580I G_kGCm'C Cuts at: 1790 2124 2592 2996 3464 4538 4685 6129 7285 Size: 334 468 404 468 1074 147 1444 1156 Cuts at: 7285 1790 Size: 2573 Fragments arranged by size: 2573 1444 1156 1074 468 468 404 334 147 BmgBI CAC'GTC Cuts at: 1318 2125 4993 1318 Size: 807 2868 4393 Fragments arranged by size: 4393 2868 807 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2710 3000 4150 Size: 416 53 83 399 24 1293 290 1150 Cuts at: 4150 4679 5799 6757 442 Size: 529 1120 958 1753 Fragments arranged by size: 1753 1293 1150 1120 958 529 416 399 290 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8068 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3061 3760 5074 3061 Size: 699 1314 6055 Fragments arranged by size: 6055 1314 699 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2125 2210 2360 2744 3167 3755 5198 5351 Size: 886 85 150 384 423 588 1443 153 Cuts at: 5351 6728 1239 Size: 1377 2579 Fragments arranged by size: 2579 1443 1377 886 588 423 384 153 150 85 Bpu10I CC'TnA_GC Cuts at: 2087 2116 2199 2982 3780 3944 5192 2087 Size: 29 83 783 798 164 1248 4963 Fragments arranged by size: 4963 1248 798 783 164 83 29 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4036 4514 6193 7058 7356 4036 Size: 478 1679 865 298 4748 Fragments arranged by size: 4748 1679 865 478 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2063 2528 5034 6731 882 Size: 617 564 465 2506 1697 2219 Fragments arranged by size: 2506 2219 1697 617 564 465 BsaAI yAC'GTr Cuts at: 493 1204 3493 5859 493 Size: 711 2289 2366 2702 Fragments arranged by size: 2702 2366 2289 711 BsaBI GATnn'nnATC Cuts at: 8062 8062 Size: 8068 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2307 2360 3870 4101 Size: 53 83 186 936 774 53 1510 231 Cuts at: 4101 5875 6257 6892 275 Size: 1774 382 635 1451 Fragments arranged by size: 1774 1510 1451 936 774 635 382 231 186 83 53 53 BsaWI w'CCGG_w Cuts at: 4590 4896 6501 6899 7173 7320 7731 4590 Size: 306 1605 398 274 147 411 4927 Fragments arranged by size: 4927 1605 411 398 306 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3059 3089 3948 3978 4310 Size: 30 165 30 1656 30 859 30 332 Cuts at: 4310 4340 4436 4466 4620 4650 5282 5312 1178 Size: 30 96 30 154 30 632 30 3934 Fragments arranged by size: 3934 1656 859 632 332 165 154 96 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2449 2642 2684 2756 2795 2804 2807 2810 Size: 1263 193 42 72 39 9 3 3 Cuts at: 2810 2813 2816 2825 2828 2831 2834 2861 2864 Size: 3 3 9 3 3 3 27 3 Cuts at: 2864 2879 2882 2885 2900 2903 2924 2927 2930 Size: 15 3 3 15 3 21 3 3 Cuts at: 2930 2933 4244 4256 4331 5024 5092 5147 5150 Size: 3 1311 12 75 693 68 55 3 Cuts at: 5150 5168 1186 Size: 18 4086 Fragments arranged by size: 4086 1311 1263 693 193 75 72 68 55 42 39 27 21 18 15 15 12 9 9 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 3226 3924 3968 4055 4107 4133 7271 7767 3226 Size: 698 44 87 52 26 3138 496 3527 Fragments arranged by size: 3527 3138 698 496 87 52 44 26 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3460 3864 5102 1163 Size: 573 1724 404 1238 4129 Fragments arranged by size: 4129 1724 1238 573 404 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3625 5518 6279 7307 1540 Size: 275 186 1624 1893 761 1028 2301 Fragments arranged by size: 2301 1893 1624 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2035 2211 2592 2988 5282 6129 6214 7285 2035 Size: 176 381 396 2294 847 85 1071 2818 Fragments arranged by size: 2818 2294 1071 847 396 381 176 85 BsmI GAATG_Cn' Cuts at: 5579 5672 5579 Size: 93 7975 BsmBI CGTCTCn'nnnn_ Cuts at: 2351 2351 Size: 8068 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2035 2124 2211 2411 2592 2988 2996 Size: 399 245 89 87 200 181 396 8 Cuts at: 2996 3464 4538 4685 4707 5282 5387 6129 6214 Size: 468 1074 147 22 575 105 742 85 Cuts at: 6214 7285 1391 Size: 1071 2174 Fragments arranged by size: 2174 1074 1071 742 575 468 399 396 245 200 181 147 105 89 87 85 22 8 BspEI T'CCGG_A Cuts at: 7731 7731 Size: 8068 BspHI T'CATG_A Cuts at: 5957 7687 5957 Size: 1730 6338 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2601 4847 5423 844 Size: 1757 2246 576 3489 Fragments arranged by size: 3489 2246 1757 576 BsrBI CCG'CTC Cuts at: 1394 1823 3045 4321 5955 1394 Size: 429 1222 1276 1634 3507 Fragments arranged by size: 3507 1634 1276 1222 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3537 6563 6737 65 Size: 1183 2289 3026 174 1396 Fragments arranged by size: 3026 2289 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3684 4191 6712 6899 1700 Size: 110 1874 507 2521 187 2869 Fragments arranged by size: 2869 2521 1874 507 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8068 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8068 BssSI C'ACGA_G Cuts at: 4611 6128 7140 4611 Size: 1517 1012 5539 Fragments arranged by size: 5539 1517 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3189 4068 4928 794 Size: 2395 879 860 3934 Fragments arranged by size: 3934 2395 879 860 BstBI TT'CG_AA Cuts at: 6872 6872 Size: 8068 BstEII G'GTnAC_C Cuts at: 1184 3751 1184 Size: 2567 5501 BstXI CCAn_nnnn'nTGG Cuts at: 3733 4877 3733 Size: 1144 6924 BstYI r'GATC_y Cuts at: 1972 2429 3295 3417 3429 3595 3627 5034 5097 Size: 457 866 122 12 166 32 1407 63 Cuts at: 5097 5398 5765 6150 6167 7619 7705 7717 8063 Size: 301 367 385 17 1452 86 12 346 Cuts at: 8063 1972 Size: 1977 Fragments arranged by size: 1977 1452 1407 866 457 385 367 346 301 166 122 86 63 32 17 12 12 Bsu36I CC'TnA_GG Cuts at: 3632 4239 3632 Size: 607 7461 BtgI C'CryG_G Cuts at: 513 1065 5076 5106 513 Size: 552 4011 30 3475 Fragments arranged by size: 4011 3475 552 30 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2366 2479 3142 487 Size: 683 56 1140 113 663 5413 Fragments arranged by size: 5413 1140 683 663 113 56 BtsI GCAGTG_nn' Cuts at: 2290 2451 3894 5584 6378 6398 2290 Size: 161 1443 1690 794 20 3960 Fragments arranged by size: 3960 1690 1443 794 161 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8068 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8033 DraI TTT'AAA Cuts at: 5372 5372 Size: 8068 DraIII CAC_nnn'GTG Cuts at: 2215 2215 Size: 8068 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7075 809 Size: 507 5759 1802 Fragments arranged by size: 5759 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2172 2565 3355 Size: 54 1425 50 275 18 342 393 790 Cuts at: 3355 3723 4429 4561 4761 4867 5515 6404 8033 Size: 368 706 132 200 106 648 889 1629 Cuts at: 8033 8 Size: 43 Fragments arranged by size: 1629 1425 889 790 706 648 393 368 342 275 200 132 106 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5515 1537 Size: 275 3703 4090 Fragments arranged by size: 4090 3703 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2174 2526 2541 2651 2772 2802 2826 2832 Size: 821 352 15 110 121 30 24 6 Cuts at: 2832 2838 2850 2856 2871 2877 2889 2895 2901 Size: 6 12 6 15 6 12 6 6 Cuts at: 2901 3272 4299 5998 1353 Size: 371 1027 1699 3423 Fragments arranged by size: 3423 1699 1027 821 371 352 121 110 30 24 15 15 12 12 6 6 6 6 6 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5216 6655 7029 7175 387 Size: 1064 138 3627 1439 374 146 1280 Fragments arranged by size: 3627 1439 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2033 2986 2033 Size: 953 7115 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2125 2210 2261 2360 2418 2550 2582 Size: 393 493 85 51 99 58 132 32 Cuts at: 2582 2744 3167 3185 3476 3755 4299 4382 4841 Size: 162 423 18 291 279 544 83 459 Cuts at: 4841 5141 5198 5351 6131 6728 7515 7783 1239 Size: 300 57 153 780 597 787 268 1524 Fragments arranged by size: 1524 787 780 597 544 493 459 423 393 300 291 279 268 162 153 132 99 85 83 58 57 51 32 18 EcoNI CCTnn'n_nnAGG Cuts at: 2597 3149 4226 4735 5195 2597 Size: 552 1077 509 460 5470 Fragments arranged by size: 5470 1077 552 509 460 EcoO109I rG'GnC_Cy Cuts at: 2050 2060 2576 2641 2704 2977 2992 3664 3961 Size: 10 516 65 63 273 15 672 297 Cuts at: 3961 4663 4785 4963 5200 5308 5323 2050 Size: 702 122 178 237 108 15 4795 Fragments arranged by size: 4795 702 672 516 297 273 237 178 122 108 65 63 15 15 10 EcoRI G'AATT_C Cuts at: 5377 5755 5377 Size: 378 7690 EcoRV GAT'ATC Cuts at: 3985 3985 Size: 8068 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2662 3772 2662 Size: 1110 6958 FspI TGC'GCA Cuts at: 3182 6574 3182 Size: 3392 4676 FspAI rTGC'GCAy Cuts at: 3182 3182 Size: 8068 HaeII r_GCGC'y Cuts at: 1678 2199 2451 2698 4114 4131 7215 1678 Size: 521 252 247 1416 17 3084 2531 Fragments arranged by size: 3084 2531 1416 521 252 247 17 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3059 3091 3313 3345 3580 Size: 32 135 32 1487 32 222 32 235 Cuts at: 3580 3612 5282 5314 6709 6741 6783 6815 1373 Size: 32 1670 32 1395 32 42 32 2626 Fragments arranged by size: 2626 1670 1487 1395 235 222 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3087 5412 5658 669 Size: 988 281 1149 2325 246 3079 Fragments arranged by size: 3079 2325 1149 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8068 HpaI GTT'AAC Cuts at: 5658 5658 Size: 8068 KpnI G_GTAC'C Cuts at: 5393 5393 Size: 8068 MfeI C'AATT_G Cuts at: 5667 5667 Size: 8068 MluI A'CGCG_T Cuts at: 7944 7944 Size: 8068 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2457 3084 3356 4281 4977 5079 5325 7182 Size: 1152 627 272 925 696 102 246 1857 Cuts at: 7182 7366 1305 Size: 184 2007 Fragments arranged by size: 2007 1857 1152 925 696 627 272 246 184 102 MscI TGG'CCA Cuts at: 10 64 1489 1832 2174 3357 3725 4563 4869 Size: 54 1425 343 342 1183 368 838 306 Cuts at: 4869 10 Size: 3209 Fragments arranged by size: 3209 1425 1183 838 368 343 342 306 54 MslI CAynn'nnrTG Cuts at: 518 1166 2297 4875 5291 6026 6385 6544 518 Size: 648 1131 2578 416 735 359 159 2042 Fragments arranged by size: 2578 2042 1131 735 648 416 359 159 MspA1I CmG'CkG Cuts at: 1606 2078 2091 2396 3226 4068 4428 4719 5023 Size: 472 13 305 830 842 360 291 304 Cuts at: 5023 5086 5484 6161 7309 7554 1606 Size: 63 398 677 1148 245 2120 Fragments arranged by size: 2120 1148 842 830 677 472 398 360 305 304 291 245 63 13 NcoI C'CATG_G Cuts at: 513 1065 5076 5106 513 Size: 552 4011 30 3475 Fragments arranged by size: 4011 3475 552 30 NdeI CA'TA_TG Cuts at: 387 3673 387 Size: 3286 4782 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8068 NotI GC'GGCC_GC Cuts at: 5515 5515 Size: 8068 NsiI A_TGCA'T Cuts at: 3444 3444 Size: 8068 NspI r_CATG'y Cuts at: 1132 1229 3802 4024 5296 5426 6971 1132 Size: 97 2573 222 1272 130 1545 2229 Fragments arranged by size: 2573 2229 1545 1272 222 130 97 PasI CC'CwG_GG Cuts at: 1348 3711 1348 Size: 2363 5705 PciI A'CATG_T Cuts at: 5292 6967 5292 Size: 1675 6393 PflMI CCAn_nnn'nTGG Cuts at: 1108 2579 2722 1108 Size: 1471 143 6454 Fragments arranged by size: 6454 1471 143 PfoI T'CCnGG_A Cuts at: 3077 3144 4808 5328 3077 Size: 67 1664 520 5817 Fragments arranged by size: 5817 1664 520 67 PmeI GTTT'AAAC Cuts at: 5372 5372 Size: 8068 PmlI CAC'GTG Cuts at: 3493 3493 Size: 8068 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4436 4468 6137 6169 7650 7682 4436 Size: 32 1669 32 1481 32 4822 Fragments arranged by size: 4822 1669 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2060 2576 2704 3664 3961 4663 4785 4963 5308 Size: 516 128 960 297 702 122 178 345 Cuts at: 5308 5323 2060 Size: 15 4805 Fragments arranged by size: 4805 960 702 516 345 297 178 128 122 15 PshAI GACnn'nnGTC Cuts at: 1613 2709 1613 Size: 1096 6972 PsiI TTA'TAA Cuts at: 5638 5638 Size: 8068 PspOMI G'GGCC_C Cuts at: 2992 2992 Size: 8068 PspXI vC'TCGA_Gb Cuts at: 3659 3659 Size: 8068 PstI C_TGCA'G Cuts at: 830 2083 2611 4073 5091 5420 830 Size: 1253 528 1462 1018 329 3478 Fragments arranged by size: 3478 1462 1253 1018 528 329 PvuI CG_AT'CG Cuts at: 2001 3625 2001 Size: 1624 6444 PvuII CAG'CTG Cuts at: 2078 2091 2396 3226 4068 5023 5086 2078 Size: 13 305 830 842 955 63 5060 Fragments arranged by size: 5060 955 842 830 305 63 13 SacI G_AGCT'C Cuts at: 2035 2988 2035 Size: 953 7115 SalI G'TCGA_C Cuts at: 1936 5410 1936 Size: 3474 4594 SanDI GG'GwC_CC Cuts at: 4785 4785 Size: 8068 SbfI CC_TGCA'GG Cuts at: 5420 5420 Size: 8068 ScaI AGT'ACT Cuts at: 2138 3023 6316 8028 2138 Size: 885 3293 1712 2178 Fragments arranged by size: 3293 2178 1712 885 SexAI A'CCwGG_T Cuts at: 2211 2211 Size: 8068 SfcI C'TryA_G Cuts at: 826 1045 2079 2473 2607 3286 4069 5087 5416 Size: 219 1034 394 134 679 783 1018 329 Cuts at: 5416 6551 7232 7423 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1034 1018 783 681 679 394 329 219 191 134 SfiI GGCCn_nnn'nGGCC Cuts at: 3722 3722 Size: 8068 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8068 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8068 SmaI CCC'GGG Cuts at: 1872 3128 4851 5395 1872 Size: 1256 1723 544 4545 Fragments arranged by size: 4545 1723 1256 544 SmlI C'TyrA_G Cuts at: 820 1017 1943 3659 4015 4529 6172 7073 7335 Size: 197 926 1716 356 514 1643 901 262 Cuts at: 7335 820 Size: 1553 Fragments arranged by size: 1716 1643 1553 926 901 514 356 262 197 SnaBI TAC'GTA Cuts at: 493 5859 493 Size: 5366 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8068 SphI G_CATG'C Cuts at: 1132 3802 4024 5426 1132 Size: 2670 222 1402 3774 Fragments arranged by size: 3774 2670 1402 222 SspI AAT'ATT Cuts at: 5 52 5992 5 Size: 47 5940 2081 Fragments arranged by size: 5940 2081 47 StuI AGG'CCT Cuts at: 1517 4169 1517 Size: 2652 5416 StyI C'CwwG_G Cuts at: 513 1065 2046 2463 3726 3747 4374 4647 5076 Size: 552 981 417 1263 21 627 273 429 Cuts at: 5076 5106 5507 513 Size: 30 401 3074 Fragments arranged by size: 3074 1263 981 627 552 429 417 401 273 30 21 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3019 3086 3519 3724 4657 4697 4849 Size: 1255 1439 67 433 205 933 40 152 Cuts at: 4849 4907 6095 6112 6265 6450 6895 325 Size: 58 1188 17 153 185 445 1498 Fragments arranged by size: 1498 1439 1255 1188 933 445 433 205 185 153 152 67 58 40 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2136 3021 5295 Size: 274 80 33 51 1443 159 885 2274 Cuts at: 5295 6314 8026 96 Size: 1019 1712 138 Fragments arranged by size: 2274 1712 1443 1019 885 274 159 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2201 2233 3948 3980 4620 4652 8028 8060 2201 Size: 32 1715 32 640 32 3376 32 2209 Fragments arranged by size: 3376 2209 1715 640 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8068 XbaI T'CTAG_A Cuts at: 5404 5404 Size: 8068 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3718 3976 5062 3718 Size: 258 1086 6724 Fragments arranged by size: 6724 1086 258 XhoI C'TCGA_G Cuts at: 1943 3659 1943 Size: 1716 6352 XmaI C'CCGG_G Cuts at: 1870 3126 4849 5393 1870 Size: 1256 1723 544 4545 Fragments arranged by size: 4545 1723 1256 544 XmnI GAAnn'nnTTC Cuts at: 6197 6197 Size: 8068 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2361 5876 276 Size: 53 83 186 936 827 3515 2468 Fragments arranged by size: 3515 2468 936 827 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BbeI BsiWI BstZ17I FseI KasI NaeI NarI NgoMIV NruI PacI PsrI RsrII SacII SapI SfoI SrfI SwaI